BMC INFECTIOUS DISEASES
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2025
·Vol. 25
·期 1
·1-9
·
IF 3
Ma Ying-Chao,Yang Xiu-Ling,Gu Jian-Jun
ObjectiveTo evaluate the effects of different SARS-CoV-2 inactivation methods on the blood concentration of colistin sulfate.Methods A colistin sulfate reference substance, a quality control plasma sample, and a clinically measured sample were transferred and heated in a 56 °C water batch for 30 min or irradiated under an ultraviolet (UV) lamp for 60 min to examine the stability of the reference solution and quality control plasma sample. Statistical analysis was conducted for the concentration of the clinically measured sample before and after inactivation with the intraclass correlation coefficient (ICC) method, the Passing–Bablok regression, and the Bland–Altman analysis.Results The reference substance of colistin sulfate was unstable under UV inactivation. Under moist heat inactivation, the reference substance and quality control plasma sample of colistin sulfate were stable. The ICC of the concentration results of 27 clinical blood samples with and without moist heat inactivation was 0.995 (P < 0.001). The Passing–Bablok regression equation was Y = 0.01347 + 0.9861X (R2 = 0.994, n = 27). Finally, 96.30% (26/27) of test data was within the 95% limit of agreement. The results obtained with the three statistical analysis methods all showed a high correlation and agreement between the plasma concentration results of samples not inactivated and inactivated with moist heat inactivation.Conclusion The clinical plasma concentration test samples for colistin sulfate can be processed with the moist heat virus inactivation method to reduce the risk of virus infection of medical staff.
研究领域:
医学
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药理学
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病毒学
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2018
·
IF 6.14
Jinxi Feng,Yao Fu,Xiaosheng Liu,Shuanghong Tian,Shenyu Lan,Ya Xiong
Piezoelectric effect was first used for disinfection. In this process, hydrothermally synthesized nano/micrometer tetragonal-BaTiO3 was selected as the piezo-catalyst which was stressed by ultrasound to generate piezoelectric potential. It was found that the inactivation number of the nano/micrometer tetragonal BaTiO3 exposure or sonication alone within 180 min was 0.30 log and 0.70 log for E. coli in deionized water, respectively, while addition of 2 g tetragonal-BaTiO3 with piezo-activity in the ultrasound system resulted in a 2.72 log inactivation without the input of any additional energy, only by original ultrasound. The latter was 2.72 folds as great as the sum of the two former inactivation numbers. However, addition of cubic-BaTiO3 without piezo-activity only led to a 1.02 log inactivation. And moreover, in the process of serious physical damage and chemical oxidation of cells, substantial leakages of intracellular materials were observed. These great differences not only clearly indicated that the combination of sonication and piezocatalysis possessed a great inactivation potential but also suggested that there was a significant synergistic inactivation effect between piezocatalytic oxidation and sono-mechanical destruction.
研究领域:
环境科学
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材料科学
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微生物学
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化学工程
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Qisen Xiang,Xiufang Liu,Junguang Li,Shengnan Liu,Hua Zhang,Yanhong Bai
This work aimed to evaluate the effects of dielectric barrier discharge (DBD) plasma on inactivation of spoilage yeast Zygosaccharomyces rouxii ( Z. rouxii ), in apple juice. Results showed that DBD plasma treatment at 90 W for 140 s resulted in about 5-log reduction of Z. rouxii in apple juice. The levels of extracellular nucleic acids and proteins as well as contents of H 2 O 2 and NO 2 − in yeast extract-peptone-dextrose (YPD) medium increased significantly after DBD plasma treatment at 90 W for 40–200 s. The increases in membrane permeability and generation of reactive species would likely contribute to DBD plasma-mediated inactivation of Z. rouxii . DBD plasma caused significant changes in pH, titratable acidity, and certain color parameters of apple juice, but had no effect on the contents of total soluble solids, reducing sugar, and total phenolics. This study provides key implications for the application of DBD plasma in fruit juice processing.
研究领域:
食品科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Infection and Drug Resistance
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2025
·
IF 2.9
Kaixuan Yuan,Benshun Tian,Annan Zhan,Zhuoxi Chen,Long Ye,Yong Ling,Suiling Liu,Xuejiao Bai,Yunhu Zhao
Purpose Inactivating filamentous fungi (molds) reduce toxin production and transmission. This study evaluated the efficacy of formic acid (FA) fumigation to identify optimal conditions and its effects on fungal morphology. We aim to develop an efficient inactivation protocol for molds and provide valuable insights into laboratory techniques for fungal inactivation.Methods Initially, 238 clinical isolates from 23 species of molds were evaluated for the inactivation efficacy of formic acid, formaldehyde, and peracetic acid. After identifying the optimal sterilizing agent, 188 isolates from 16 clinically significant species were tested. Four concentrations (30%, 50%, 70%, and 100%) and six-time intervals (4 to 14 hours) were tested to optimize fumigation conditions. Morphological changes were analyzed using Aspergillus fumigatus ATCC MYA-3626 as the standard strain.Results The inactivation rates of 238 molds fumigated with formic acid, formaldehyde, and peracetic acid for 24 hours were 99.16%, 89.08%, and 90.35% (P < 0.05), indicating that formic acid was more effective than formaldehyde and peracetic acid. Subsequently, 188 isolates from 16 species were fumigated with formic acid at concentrations of 30%, 50%, 70%, and 100% for 24 hours, resulting in inactivation rate of 73.9%, 98.4%, 100%, and 100% (P < 0.05). Inactivation rate increased significantly with longer exposure times: 51.1% at 4 hours, 80.9% at 6 hours, 89.4% at 8 hours, 94.7% at 10 hours, 100% at 12 hours, and 100% after 14 hours (P < 0.05). After fumigation, A. fumigatus ATCC MYA-3626 growth ceased. Electron microscopy revealed irregular folds and roughness on spore surfaces, as well as collapsed or dead spores.Conclusion Formic acid demonstrated superior inactivation efficacy compared to formaldehyde and peracetic acid. At a concentration of 70% and a fumigation duration of 12 hours, the inactivation of molds was most effective.
研究领域:
真菌学
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微生物学
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临床实验室技术
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真菌病理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Huimin Zhao,Aiping Yang,Nan Zhang,Shiyang Li,Tianjie Yuan,Ning Ding,Siwang Zhang,Sheng Bao,Chang Wang,Yinan Zhang,Xiachang Wang,Lihong Hu
The discovery of new, safe, and effective pesticides is one of the main means for modern crop protection and parasitic disease control. During the search for new insecticidal secondary metabolites from endophytes in Stemona sessilifolia (a traditional Chinese medicine with a long history as an insecticide), 10 new insecticidal endostemonines A–J (1–10) were identified from an endophytic Streptomyces sp. BS-1. Their structures were determined by comprehensive spectroscopic analysis. Endostemonines A–J represent the first reported naturally occurring pyrrole-2-carboxylic ester derivatives, which consisted of different fatty acid chains at the C-2 of pyrrole ring were produced by traditional Chinese medicine endophytic microbes. All new tested compounds exhibited strong lethal activity against Aphis gossypii (LC50 value range of 3.55–32.00 mg/L after 72 h). This research highlighted the discovery of pesticide natural products from insecticidal medicinal plant endophytes for the first time, paving a new pathway for the development of pest control.
研究领域:
植物学
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农药开发
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微生物学
✓ 已验证
DOI
PubMed
高影响因子
WATER RESEARCH
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2025
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IF 12.4
Yuqing Hu,Nuanqin Zhang,Xiang Zhang,Junning Zu,Sicong Ma,Linghao Yu,Chang Guo,Xiaomei Liao,Shaohui Wang,Hao Li,Hongwei Sun,Lizhi Zhang
The microbe-surface interaction plays essential roles in various natural and anthropogenic water processes, and it is of great benefit to tailor their interfacial contact. For instance, zero-valent iron (ZVI) holds great promise as an emerging disinfection agent, but its efficiency remains limited due to the insufficient ZVI-bacteria contact. Herein we modified the surface of ZVI with potassium dihydrogen phosphate (KH 2 PO 4 ) by ball milling (P-ZVI bm ) to facilitate the close adhesion and inactivation of Escherichia coli ( E. coli ). ExDLVO simulation indicated the significantly alleviated Lewis acid-base repulsion between P-ZVI bm and E. coli due to the enhanced hydrogen bonding. ATR-FTIR spectra and DFT calculation suggested that the surface phosphate moiety could form dual hydrogen bonds with the amide domain of bacterial proteins (e.g., membrane proteins, pili, flagella, etc.), significantly shortening the length of hydrogen bonds from 1.784 to 1.781 and 1.646 Å. The enhanced adhesion of E. coli on P-ZVI bm lead to more pronounced oxidative stress and inactivation of the cells. Impressively, P-ZVI bm exhibited superior bactericidal efficiency of 99.2% in batch experiments, far surpassing that of ZVI bm (33.9%, p < 0.001). As a prototype of application, the sand column packed with P-ZVI bm continuously removed E. coli to below 100 CFU/mL from simulated groundwater. This study provided an effective and facile strategy of phosphorylation to modulate the ZVI-bacterium adhesion for contact sterilization, and may shed light on the development of novel disinfection techniques, and the design of biofilm carriers for wastewater treatment, etc.
研究领域:
环境科学
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微生物学
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水处理工程
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产品关联
DOI
PubMed
高影响因子
Journal of Water Process Engineering
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2024
·
IF 6.3
Miao Yu,Xinyuan Lv,Ziqi Wang,Minghao Sui
Virus-induced risks to water quality persist, with the viability of viruses in complex aquatic environments impacted by various factors, including coexisting bacteria and exposure to light. This study investigated the disinfection efficacy of UV 254 irradiation and free chlorine (FC) on Bacteriophage phiX174 in the presence of coexisting bacteria, specifically Escherichia coli ( E. coli ) and Bacillus subtilis ( B. subtilis ). To elucidate the underlying mechanisms, this study examined the effects of bacterial compounds (i.e., E. coli lipopolysaccharide (LPS) and B. subtilis peptidoglycan (PGN)) on viral inactivation, thermal stability, and environmental persistence. The research results demonstrated that bacterial presence and their associated compounds significantly impaired viral inactivation, with greater concentrations of coexisting bacteria and their compounds exacerbating this effect. Additionally, the photoreactivation of phiX174 was explored following UV 254 irradiation, indicating that high-dose UV 254 , coupled with low nutrient availability and minimal host bacteria levels, effectively suppressed viral photoreactivation. This study is the first to investigate the multifactorial effects on viral photoreactivation. The results offer novel insights into the challenges associated with virus-related pressures in water treatment, with potential implications for optimizing disinfection processes.
研究领域:
环境科学
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微生物学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Xinxin Wang,Shan Sun,Ke Cheng,Tao Lin,Henggang Wang,Haoyi Sun,Hengwei Lin,Peng Wang
Pathogenic bacterial spores pose a considerable threat to human health due to their ability to survive extreme environmental conditions. Thus, sensitive detection and efficient inactivation of these spores are essential for preventing disease transmission. However, most existing studies have predominantly concentrated on detection alone. Herein, we developed a versatile nanoplatform that integrates sensitive bacterial spore sensing and in situ spore inactivation capabilities by assembling terbium ions (Tb 3+ ) with photosensitizing carbon dots (CDs), referred to as Tb-CDs nanoassemblies (NAs). By leveraging the unique optical properties of CDs and Tb 3+ ions, along with the antenna effect of lanthanides, Tb-CDs NAs demonstrated highly sensitive visual detection of the key bacterial spore biomarker, pyridinedicarboxylic acid (DPA), achieving a low detection limit of 0.68 μM and excellent selectivity. Additionally, Tb-CDs NAs exhibited practical sensing capabilities in various environments, including spore suspensions and real samples, while monitoring bacterial spore germination. Further studies confirmed that the DPA-triggered disassembly of Tb-CDs NAs led to the release of CDs, enabling higher affinity for spores and in situ inactivation through singlet oxygen generation. This work underscores the potential of CDs-based assemblies for simultaneous pathogen detection and inactivation, providing promising tools for preventing bacterial spread and contamination.
研究领域:
生物传感
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微生物学
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纳米医学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Xing Gao,Weiying Feng,Hanxiao Zhang,Nanyan Weng,Shouliang Huo
Harmful cyanobacterial blooms in eutrophic water bodies have frequently occurred worldwide and become a major environmental concern. Therefore, it is imperative to develop a stable and efficient algaecide to solve this issue. In this study, our purpose was to investigate the efficacy and mechanism of a newly developed Cu 2+ doped Fe 3 O 4 @Biochar magnetic composite (Cu-Mag-BC) in in-situ inactivation of Microcystis aeruginosa ( M. aeruginosa ) . We successfully synthesized the Cu-Mag-BC by coating Cu 2+ onto Fe 3 O 4 @Biochar. Cu-Mag-BC exhibited superparamagnetic behavior and was uniformly impregnated by Cu 2+ . Cu-Mag-BC (5 mg/L), rapidly inactivated chlorophyll-a (Chl-a) in M. aeruginosa with low Fe and Cu leaching, during which time the OD 264 value and malondialdehyde (MDA) content increased, while the activities of superoxide dismutase (SOD) and catalase (CAT) first increased and then decreased, due to oxidative stress induced by over-generated reactive oxygen species (ROS). Quantitative results showed that ·O 2 − and ·OH were the main ROS species produced from Cu-Mag-BC. Inactivation efficiency was maintained at approximately 80 % after three consecutive runs and total Chl-a removal efficiency reached 2.84 g/g, indicating good reusability and stability. A possible inactivation mechanism is proposed; amino groups and adipose chain were the primary oxidation sites. Thus, Cu-Mag-BC shows potential as a candidate for simultaneously inactivating harmful cyanobacteria and preventing secondary pollution.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD MICROBIOLOGY
·
2024
·
IF 4.5
Qiaoyun Wei,Yuan Yuan,Jianhao Zhang,Jin Wang
This study investigated the fungicidal efficiency and mechanism of action of dielectric barrier discharge cold atmosphere plasma (DBD-CAP) in inactivating Aspergillus niger ( A. niger ) spores. The disinfection efficacy and quality of dried jujube used as the processing application object were also studied. The results indicated that the Weibull + Tail model performed better for spore inactivation curves at different voltages among various treatment times, and the spore cells were reduced by 4.05 log (cfu/mL) in spores suspension at 70 kV after 15 min of treatment. This disinfection impact was further supported by scanning electron microscope (SEM) and transmission electron microscopy (TEM) images, which showed that the integrity of the cell membrane was damaged, and the intracellular content leaked out after DBD-CAP treatment. Elevated levels of reactive oxygen species (ROS) during the treatment increased the relative conductivity of cells, and leakage of nucleic acids and proteins further supported the disinfection impact. Additionally, the growth and toxicity of surviving A. niger spores after treatment were also greatly reduced. When DBD-CAP was applied to disinfecting dried jujube, the spore number exhibited a 2.67 log cfu/g reduction after treatment without significant damage observed onto the quality ( P > 0.05 ).
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Nano
·
2020
·Vol. 7
·期 12
·3877-3887
·
IF 7.683
Wanjun Wang,Wenquan Gu,Guiying Li,Haojing Xie,Po Keung Wong,Taicheng An
Sustainable and “green” technologies for hydrogen peroxide (H2O2) production have aroused increasing interest, yet challenges prevail in production of H2O2 for environmental applications. In this study, tungsten selenide (WSe2) with a few-layered microstructure was used as a new co-catalyst to synthesize H2O2 under visible light irradiation. The ultrathin WSe2 nanosheets were anchored onto a g-C3N4 surface by in situ growth solvothermal methods. The as-prepared WSe2/g-C3N4 showed remarkably enhanced H2O2 production efficiency without organic electron donors, which was 11.8 times higher than that of pristine g-C3N4. H2O2 was generated via O2 reduction mediated by photogenerated e−, and dissolved O2 could be generated by water oxidation and in situ used to produce H2O2 under anaerobic conditions. The introduction of WSe2 not only promoted visible light absorption, but also facilitated the charge transfer efficiency by forming a Z-scheme rather than a type II heterojunction. The photogenerated e− was enriched onto WSe2, leading to the high H2O2 production activity. Moreover, the in situ generated H2O2 could be directly used for pathogenic bacteria inactivation through Fenton reactions without external H2O2 addition. This work is expected to open an avenue for developing novel photocatalysts towards sustainable H2O2 production for water disinfection and related environmental applications.
研究领域:
环境科学
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材料科学
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光化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yongsheng Ni,Yumeng Li,Mengyi Wang,Haoran Li,Wendi Zhang,Lijun Tan,Jinsong Zhao,Baocai Xu
The current food packaging films can be preservative but lack the function of combining antibacterial and sterilization which lead to films can not maximize prolong shelf life of perishable foods. This study provided a new strategy to realize prolonging shelf life of perishable foods by integrating antibacterial and sterilization which focused on applying photodynamic inactivation to films with continuous activity, where curcumin (CUR) and sodium copper chlorophyll (SCC) were loaded into chitosan (CS) films. Compared to pure CS films, the barrier capacity (oxygen permeability and water vapor permeability) and mechanical properties of composite films were improved by introducing CUR and SCC. In addition, the composite film can effectively against food-borne pathogenic bacteria and significantly prolong the shelf life of cherries and pork. The provided strategy has potential application prospects in food preservation packaging.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）