Waste and Biomass Valorization
·
2020
·Vol. 11
·期 12
·6555-6563
·
IF 2.851
Hou Rongrong,Shi Jie,Ma Xiubing,Wei Huanran,Hu Jiajun,Tsang Yiu Fai,Gao Min-Tian
Abstract Phenolic compounds are widely used in different research fields, such as pesticides, medicines, and food. In this study, phenolic acids (PAs) were extracted from rice straw and were found to exhibit a strong inhibitory effect on Botrytis cinerea . B. cinerea mycelial growth and spore generation decreased by 86.18% and 69.10%, respectively, following 0.2 g/L phenolic acid treatment. Confocal microscopic images demonstrated that phenolic acids changed the morphology of B. cinerea . The addition of phenolic acids to B. cinerea- infected tomato leaves increased PAL (phenylalaninammo-nialyase) and PPO (polyphenol oxidase) activities, and decreased POD (peroxidases) and CAT (catalase) activities in the leaves, indicating that phenolic acids enhanced the tolerance of tomato leaves to B. cinerea by reducing oxidative stress. Chlorophyll fluorescence imaging revealed that phenolic acids could alleviate the destruction of the photosynthetic system of B. cinerea -infected leaves. These results provide new insight into the use of phenolic acids from rice straw, through which a complete green cycle of ecological production can be established. Graphic Abstract
研究领域:
植物病理学
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农业科学
✓ 已验证
产品关联
DOI
高影响因子
Plants-Basel
·
2023
·Vol. 12
·期 10
·1992
·
IF 4.5
Yongyao Fu,Liling Shu,Hanyi Li,Xingming Zhang,Xuan Liu,Zhengying Ou,Xiaomeng Liang,Xiangying Qi,Liping Yang
Lilium browniivar.viridulum, commonly called Longya lily, is a well-known flower and vegetable plant in China that has poor tolerance toBotrytisfungal disease. The molecularimprovement has mainly been restricted to an efficient regeneration and transformation system. In this study, the highly efficient regeneration of Longya lily was established through the optimization of embryogenic callus, adventitious shoot and rooting induction. The major factors influencing transformation (antibiotics,Agrobacteriumconcentration, infection time, suspension solution and coculture medium) were examined. The expression responses of PR promoters (ZmPR4andBjCHI1) toB. cinereawere assessed in transgenic calli. The results showed that Murashige and Skoog (MS) medium with 1.0 mg·L−1picloram (PIC) and 0.2 mg·L−11-naphthaleneacetic acid (NAA) under light conditions and MS with 0.5 mg·L−16-benzylaminopurine (6-BA) and 1.0 mg·L−1NAA under darkness were optimal for embryogenic callus induction (64.67% rate) and proliferation (3.96 coefficient). Callus inoculation into MS containing 2.0 mg·L−1thidiazuron (TDZ), 0.4 mg·L−1NAA, 1.0 mg·L−1TDZ and 0.5 mg·L−1NAA led to shooting induction (92.22 of rate) and proliferation (3.28 of coefficient) promotion, respectively. The rooting rate reached 99.00% on MS with 0.3 mg·L−1NAA. Moreover, a transformation rate of 65.56% was achieved by soaking the callus inAgrobacteriumat an OD600of 0.4 for 10 min in modified MS without NH4NO3as the suspension solution and coculture medium before selecting 75 mg·L−1hygromycin and 300 mg·L−1cefotaxime. Only theBjCHI1promoter was obviously expressed in transgenic calli. These results could facilitate the generation of Longya lily transgenic plants with improvedB. cinerearesistance.Keywords:Lilium browniivar.viridulum;plant regeneration;callus;genetic transformation;Botrytis cinerea
研究领域:
植物学
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遗传学
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植物病理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Quan Ma,Dong Li,Yicheng Ren,Yanpei Chen,Jing Huang,Bin Wu,Qingqing Wang,Zisheng Luo
Autophagy (AU) and programmed cell death (PCD) are dynamically regulated during tomato fruit defense against Botrytis cinerea , which are also manipulated by pathogenic effectors to promote colonization. Present study demonstrated that the enhanced defense induced by transient inhibition on AU by hydroxychloroquine (HCQ) facilitated the restriction of B. cinerea lesion on postharvest tomato. Pre-treatment of 2 mM (16.08 ± 3.42 cm at 7 d) and 6 mM (7.80 ± 2.39 cm at 7 d) HCQ inhibited the lesion development of B. cinerea compared with Mock treatment (50.02 ± 7.69 cm at 7 d). Transient inhibition of AU induced expression of fungal defense and transcriptional regulation related genes, but attenuated reactive oxygen species (ROS) burst gene expression. The ROS-induced PCD was compromised by HCQ with promoted ROS scavenging. The transient pre-treatment of HCQ slightly inhibited AU which triggered the feedback loop that enhanced the autophagic activity defensing against B. cinerea infection.
研究领域:
植物病理学
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分子生物学
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农业科学
✓ 已验证
DOI
PubMed
高影响因子
Microbial Cell Factories
·
2024
·Vol. 23
·期 1
·1-16
·
IF 4.3
Wang Yifan,Shu Dan,Li Zhemin,Luo Di,Yang Jie,Chen Dongbo,Li Tianfu,Hou Xiaonan,Yang Qi,Tan Hong
Background Currently, industrial fermentation of Botrytis cinerea is a significant source of abscisic acid (ABA). The crucial role of ABA in plants and its wide range of applications in agricultural production have resulted in the constant discovery of new derivatives and analogues. While modifying the ABA synthesis pathway of existing strains to produce ABA derivatives is a viable option, it is hindered by the limited synthesis capacity of these strains, which hinders further development and application. Results In this study, we knocked out the bcaba4 gene of B. cinerea TB-31 to obtain the 1′,4′- trans -ABA-diol producing strain ZX2. We then studied the fermentation broth of the batch-fed fermentation of the ZX2 strain using metabolomic analysis. The results showed significant accumulation of 3-hydroxy-3-methylglutaric acid, mevalonic acid, and mevalonolactone during the fermentation process, indicating potential rate-limiting steps in the 1′,4′- trans -ABA-diol synthesis pathway. This may be hindering the flow of the synthetic pathway. Additionally, analysis of the transcript levels of terpene synthesis pathway genes in this strain revealed a correlation between the bchmgr , bcerg12 , and bcaba1-3 genes and 1′,4′- trans -ABA-diol synthesis. To further increase the yield of 1′,4′- trans -ABA-diol, we constructed a pCBg418 plasmid suitable for the Agrobacterium tumefaciens -mediated transformation (ATMT) system and transformed it to obtain a single-gene overexpression strain. We found that overexpression of bchmgr , bcerg12 , bcaba1 , bcaba2 , and bcaba3 genes increased the yield of 1′,4′- trans -ABA-diol. The highest yielding ZX2 A3 strain was eventually screened, which produced a 1′,4′- trans -ABA-diol concentration of 7.96 mg/g DCW (54.4 mg/L) in 144 h of shake flask fermentation. This represents a 2.1-fold increase compared to the ZX2 strain. Conclusions We utilized metabolic engineering techniques to alter the ABA-synthesizing strain B. cinerea , resulting in the creation of the mutant strain ZX2, which has the ability to produce 1′,4′- trans -ABA-diol. By overexpressing the crucial genes involved in the 1′,4′- trans -ABA-diol synthesis pathway in ZX2, we observed a substantial increase in the production of 1′,4′- trans -ABA-diol.
研究领域:
生物技术
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微生物学
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代谢工程
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MICROBIOLOGY
·
2013
·Vol. 51
·期 2
·166-173
·
IF 1.276
Wu Qiong,Bai Linquan,Liu Weicheng,Li Yingying,Lu Caige,Li Yaqian,Fu Kehe,Yu Chuanjin,Chen Jie
Streptomyces lydicus A01 and Trichoderma harzianum P1 are potential biocontrol agents of fungal diseases in plants. S. lydicus A01 produces natamycin to bind the ergosterol of the fungal cell membrane and inhibits the growth of Botrytis cinerea. T. harzianum P1, on the other hand, features high chitinase activity and decomposes the chitin in the cell wall of B. cinerea . To obtain the synergistic biocontrol effects of chitinase and natamycin on Botrytis cinerea , this study transformed the chit42 gene from T. harzianum P1 to S. lydicus A01. The conjugal transformant (CT) of S. lydicus A01 with the chit42 gene was detected using polymerase chain reaction (PCR). Associated chitinase activity and natamycin production were examined using the 3, 5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry, respectively. The S. lydicus A01- chit42 CT showed substantially higher chitinase activity and natamycin production than its wild type strain (WT). Consequently, the biocontrol effects of S. lydicus A01- chit42 CT on B. cinerea , including inhibition to spore germination and mycelial growth, were highly improved compared with those of the WT. Our research indicates that the biocontrol effect of Streptomyces can be highly improved by transforming the exogenous resistance gene, i.e. chit42 from Trichoderma , which not only enhances the production of antibiotics, but also provides a supplementary function by degrading the cell walls of the pathogens.
研究领域:
微生物学
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植物病理学
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基因工程
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Laifeng Lu,Lifeng Ji,Qingqing Ma,Mingguan Yang,Shuhua Li,Qiong Tang,Liping Qiao,Fengjuan Li,Qingbin Guo,Changlu Wang
The acquisition of susceptibility to necrotrophy over the course of ripening is one of the critical factors limiting shelf life. In this study, phytopathology and molecular biology were employed to explore the roles of pectinase in fruit susceptibility and ripening. Solanum lycopersicum fruit softened dramatically from entirely green to 50% red, which was accompanied by a continuously high expressed SlPG2 gene. The necrotrophic fungus Botrytis cinerea further activated the expression of SlPGs and SlPMEs to accelerate cell wall disassembly, while most of the polygalacturonase inhibitor proteins encoding genes expression were postponed in ripe fruit following the pathogen attack. Pectin induced the antagonistic yeast to secrete pectinolytic enzymes to increase fruit resistance against gray mold. The activities of pathogenic pectinase of B. cinerea were correspondingly depressed in the pectin-inducible yeast enzyme elicited ripe fruit. These data suggest that pectinase is a molecular target for regulation of disease resistance during fruit ripening.
研究领域:
植物病理学
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分子生物学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2023
·
IF 4.7
Shengnan Shi,Ju Wang,Changhong Liu,Lei Zheng
Quercetin is a kind of flavonoid substance extensively existing in the plant, which has antioxidant, anti-inflammatory, and anti-apoptosis effects. It was reported that the higher concentration of spores present in the environment could cause abnormal development in zebrafish larvae. Therefore, this study set out to investigate whether quercetin could reduce the zebrafish larvae damage caused by Botrytis cinerea exposure as well as to examine the molecular basis for this action. The findings demonstrated that 50 μM quercetin improved the developmental dysplasia of zebrafish larvae induced by 10 2 CFU/mL Botrytis cinerea spore suspension, reduced abnormal apoptosis, enhanced antioxidant system, relieved inflammation, reshaped intestinal morphology and recovered intestinal motility . At the molecular level, quercetin decreased the transcriptional abundance of pro-apoptotic factors ( bax , p53 , caspase3 , and caspase9 ) and up-regulated the anti-apoptotic gene ( bcl-2 ) expression to reduce apoptosis. Moreover, quercetin enhanced the activities of downstream antioxidant enzymes (SOD and CAT) to clear excess ROS and MDA due to Botrytis cinerea exposure by up-regulating the expression of antioxidant genes ( nrf2 , ho-1 , sod , and cat ) in the Keap1-Nrf2 pathway. Additionally, quercetin inhibited the elevation of TNF-α by regulating the gene expression of key targets ( jak3 , pi3k , pdk1 , akt , and ikk2 ) and the content of major proteins NF-κB (P65) and IκB in the NF-κB pathway. In conclusion, this work enriched the contents of the biological research of Botrytis cinerea and provided a new direction for the drug development and targeted therapy of quercetin.
研究领域:
药理学
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毒理学
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动物学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Xiaomin Zhao,Yunfei Zhang,Li Chen,Zhiqing Ma,Bin Zhang
The practical application of essential oils (EOs) as an alternative for synthetic pesticides in agricultural production is severely limited because of their instability, high volatility, and water insolubility. Nanoencapsulation of EOs is an important strategy to overcome these limitations. In view of this, this study aimed to develop chitosan-thymol nanoparticle (NCS-Thy) with pH-responsive which can be used as an intelligent botanical fungicide to control Botrytis cinerea . The NCS-Thy nanoparticle was prepared by ionic crosslinking method with the loading capacity and encapsulation efficiency of 29.87% and 41.92%, respectively. The synthesized NCS-Thy nanoparticle was further characterized by Fourier transform infrared spectroscopy analysis, transmission electron microscopy observation, and dynamic lights scattering . The results of release kinetics and antifungal activity of NCS-Thy under different pH conditions were determined. The results showed that the NCS-Thy nanoparticle had excellent pH-responsiveness and can release more thymol under acidic conditions formed by B. cinerea , thereby achieving higher antifungal effects. Therefore, compared with unencapsulated thymol, the NCS-Thy nanoparticle had higher antifungal activity against B. cinerea in vitro . In addition, both the protective and curative efficacies of detached leaf test and pot experiment were significantly higher than those of unencapsulated thymol. Among them, the protective efficacy of NCS-Thy in the pot experiment was 78.73%, which was significantly higher than that of unencapsulated thymol with 61.13%. Therefore, the pH-responsive chitosan-thymol nano-preparation had a promising prospect of application in practical management of gray mold as an intelligent botanical fungicide.
研究领域:
农业科学
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纳米技术
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植物病理学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Menglu Wu,Zi-An Deng,Chaoyi Shen,Zhichao Yang,Zihan Cai,Di Wu,Kunsong Chen
Fruit is susceptible to various postharvest pathogens; thus, the development of multifunctional preservation materials that can achieve the broad-spectrum inhibition of different pathogens is a current research hotspot. Here, microfluidic blow spinning was used to create a biodegradable polycaprolactone/ethyl cellulose (PCL/EC) nanofibrous film that incorporated two naturally-sourced compounds, natamycin and trans -cinnamic acid, resulting in multi-microbial inhibition. The PCL/EC-based film had a smooth and even morphology, indicating the favorable integration of PCL and EC. After the incorporation of ingredients, the film exhibited good inhibitory activity against Escherichia coli , Staphylococcus aureus , and Botrytis cinerea , and it had finer fiber diameters, higher permeability, and antioxidant properties. We further demonstrated that strawberries that were padded with the film had good resistance to Botrytis cinerea . Also, the film did not interference with the qualities of the strawberries during storage. The study demonstrates a promising application for multi-antimicrobial and bio-friendly packaging materials in postharvest fruit preservation.
研究领域:
材料科学
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食品科学
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生物技术
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Packaging and Shelf Life
·
2023
·
IF 8
Liping Zhou,Mingda Hao,Tiantian Min,Xiaochun Bian,Haiyu Du,Xiaoli Sun,Zhu Zhu,Yongqiang Wen
This study aimed to develop an antimicrobial food preservation material based on kaolin (KL) encapsulated with thyme essential oil (TEO). The release of TEO in the TEO/KL composite was controlled by the relative humidity inside of food packaging. The morphology and element distribution of TEO/KL composites were determined with scanning electron microscopy and energy dispersive spectrometer. The release profiles illustrated that the release of TEO accelerated with the increase of relative humidity (30%, 60% and 90%) due to the high porosity and hydrophilicity of KL. The antimicrobial results illustrated that the activity of Escherichia coli ( E. coli ), Staphylococcus aureus ( S. aureus ) and Botrytis cinerea ( B. cinerea ) remarkably reduced after treating with the TEO/KL composite (1.0, 1.5 and 2.0 mg), and the inhibition rate reached over 99%. The cytotoxicity assays demonstrated good biocompatibility and biosafety of TEO/KL applied to food preservation. This composite also exhibited a desirable preservation effect on strawberries and grapes and displayed great potential in food industry.
研究领域:
食品科学
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材料科学
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微生物学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
Horticulture Research
·
2022
·Vol. 10
·期 1
·
IF 7.291
Hu Zhangjian,Shi Junying,Feng Shuxian,Wu Xiaodan,Shao Shujun,Shi Kai
The ubiquitous lipid-derived molecules N-acylethanolamines (NAEs) have multiple immune functions in mammals, but their roles and mechanisms in plant defense response during changing environment remain largely unclear. Here, we found that exogenous NAE18:0 and NAE18:2 promoted defense against the necrotrophic pathogen Botrytis cinerea but suppressed defense to the hemi-biotrophic pathogen Pseudomonas syringae pv. tomato (Pst) DC3000 in tomato. The knocking-down and overexpression function analysis of the pathogen-responsive NAE synthetic gene PHOSPHOLIPASE Dγ (PLDγ) and hydrolytic gene FATTY ACID AMID HYDROLASE 1 (FAAH1) revealed that the NAE pathway is crucial for plant defense response. Using exogenous applications and SA-abolished NahG plants, we unveiled the antagonistic relationship between NAE and SA in plant defense response. Elevated CO2 and temperature significantly changed the NAE pathway in response to pathogens, while inhibition of the NAE pathway led to the alternation of environment-mediated defense variations against Pst DC3000 in tomato, indicating that NAE pathway is associated with plant defense variations in response to elevated CO2 and temperature. The results herein reveal a new function of NAE in plant defense, and its involvement in environment-mediated defense variation in tomato. These findings shed light on the NAE-based plant defense, which may have relevance to crop disease management in future changing climate.
研究领域:
植物科学
·
分子生物学
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环境科学
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农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Laifeng Lu,Ying Yang,Haoran Zhang,Dandan Sun,Zhenjing Li,Qingbin Guo,Changlu Wang,Liping Qiao
Mechanical wounding causes significant economic losses of fresh produce due to accelerated senescence and spoilage as well as loss of nutritional value. Here, pre-application of oligogalacturonides (OGs) enzymatically hydrolyzed from apple pectin effectively reduced the healing times of mechanical wounds from>24 h in mock groups to 12 h, and the Botrytis cinerea infection rate was reduced from 37.5% to 12.5%. OGs accordingly increased callose deposition; SlPR1, SlPAL and SlHCT gene expression; and phenylalanine ammonia-lyase (PAL) activity around the wounds. Inhibition of Ca 2+ signaling using the inhibitor Ruthenium Red markedly inhibited OG accelerated healing of mechanical wounding on fruit. SlPG2, SlEXP1 , and SlCEL2 mRNAs accumulation was reduced in OG-elicited tomato fruit compared to water-treated fruit with subsequent retardation of the fruit softening during ripening. These results indicated that apple pectin OGs accelerate wound healing and inhibit fruit softening by activating calcium signaling in tomato fruits during postharvest storage.
研究领域:
植物科学
·
采后生理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）