JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2010
·
IF 2.453
Na Ding,Qing Yang,Sha-Sheng Huang,Liu-Yin Fan,Wei Zhang,Jian-Jiang Zhong,Cheng-Xi Cao
Ganoderic acids (GAs) were bioactive secondary metabolites produced by a traditional mushroom Ganoderma lucidum . We describe a simple and efficient method for the separation and quantitative determination of four GAs, namely Ganoderic acid T (GA-T), Ganoderic acid Mk (GA-Mk), Ganoderic acid Me (GA-Me) and Ganoderic acid S (GA-S) from dried triterpene-enriched extracts of G. lucidum mycelia powder by capillary zone electrophoresis (CZE). Under the optimum conditions, the four GAs reached the baseline separation in 9 min with Glycyrrhetinic acid (GTA) as internal standard. The four GAs and internal standard (GTA) were detected at a wavelength 245 nm. All calibration curves showed good linearity ( r 2 > 0.9958) within test ranges. Limit of detection (LOD) and limit of quantification (LOQ) were less than 0.6 and 1.8 μg/mL, respectively. The relative standard deviation (R.S.D.) values of precision and recoveries were less than 5% and recoveries ranged from 91.4% to 103.6%. This was the first report on simultaneous determination of the four GAs and the results provided a firm basis for the trace analysis of GAs in dried fermentation mycelia powder of G. lucidum with high accuracy.
研究领域:
植物化学
·
分析化学
·
药用真菌研究
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD QUALITY
·
2020
·
IF 1.763
Shengyun Li,Yaowu Yuan,Chenchen Yu,Hao Gao,Jianxin Tan,Yiling Tian
A method for the quantitative determination of ganoderic acid A was constructed using the principle of indirect competitive enzyme-linked immunosorbent assay (ELISA), and this method was used to determine the ganoderic A contents of Ganoderma lucidum samples in the market. The conjugate of ganoderic acid A and bovine serum albumin was used for four rounds of immunization on test rabbits to obtain rabbit antiganoderic acid A antibody IgG. The enzyme-labeled plate was coated with the conjugate of ganoderic acid A and ovalbumin. The first stage reaction in the indirect competitive ELISA was that the conjugate of ganoderic acid A in the sample competed with the conjugate coated on the enzyme-labeled plate to bind rabbit antibodies. The second stage reaction was the combination of goat anti-rabbit IgG–horseradish peroxidase and rabbit antiganoderic acid A antibody IgG. The results of the determination of ganoderic acid A standard by this method showed that the coefficient of variation of repeated wells in the group was <5%, the detection limit of ganoderic acid A was 0.6 μg/L, and ganoderic acid A had a substantial dose-response relationship in the content range of 0.9–72.9 μg/L (R2 = 0.994). This method was used to measure the ganoderic A content of 12 varieties of G. lucidum in the market and showed the obvious differences in the ganoderic acid A contents of the different varieties. This method is simple, fast, and of great importance to the quality control of Ganoderma products.
研究领域:
分析化学
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药理学
·
真菌学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Qiang-Ming Li,Hui-Hui Han,Dan-Dan Zang,Xue-Qiang Zha,An Zhou,Feng-Yun Zhang,Jian-Ping Luo
An amyloid-β (Aβ) fibril is a vital pathogenic factor of Alzheimer’s disease (AD). Aβ fibril disintegrators possess great potential to be developed into novel anti-AD agents. Here, a ligand fishing method was employed to rapidly discover Aβ42 fibril disintegrators from Ganoderma lucidum using Aβ42 fibril-immobilized magnetic beads, which led to the isolation of six Aβ42 fibril disintegrators including ganodermanontriol, ganoderic acid DM, ganoderiol F, ganoderol B, ganodermenonol, and ergosterol. Neuroprotective evaluation in vitro exhibited that these Aβ42 fibril disintegrators could significantly mitigate Aβ42-induced neurotoxicity. Among these six disintegrators, ergosterol and ganoderic acid DM with stronger protecting activity were further selected to evaluate their neuroprotective effect on AD in vivo. Results showed that ergosterol and ganoderic acid DM could significantly alleviate Aβ42-induced cognitive dysfunction and hippocampus neuron loss in vivo. Moreover, ergosterol and ganoderic acid DM could significantly inhibit Aβ42-induced neuron apoptosis and Nrf2-mediated neuron oxidative stress in vitro and in vivo.
研究领域:
神经科学
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药理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2020
·Vol. 104
·期 16
·7079-7091
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IF 3.53
Wang Shengli,Han Jing,Xia Jiale,Hu Yanru,Shi Liang,Ren Ang,Zhu Jing,Zhao Mingwen
Abstract Identifying new and economical means to utilize diverse lignocellulosic biomass is an urgent task. Ganoderma lucidum is a well-known edible and medicinal basidiomycete with an excellent ability to degrade a wide range of cellulosic biomass, and its nutrient use efficiency is closely related to the activity of extracellular cellulase. Intracellular nicotinamide adenine dinucleotide (NAD + ) biosynthesis is controlled in response to nutritional status, and NAD + is an essential metabolite involved in diverse cellular processes. Nicotinamide mononucleotide adenylyltransferase (NMNAT) is a common enzyme in three NAD + synthesis pathways. In this study, a homologous gene of nmnat was cloned from G. lucidum and two G. lucidum overexpression strains, OE::nmnat4 and OE::nmnat19, were constructed using an Agrobacterium tumefaciens -mediated transformation method. The G. lucidum nmnat overexpression strains showed obviously increased colony growth on different carbon sources, and intracellular Ca 2+ concentrations in the G. lucidum OE::nmnat4 and OE::nmnat19 strains were increased by 2.04- and 2.30-fold, respectively, compared with those in the wild-type (WT) strains. In the G. lucidum OE::nmnat4 and OE::nmnat19 strains, endo- β -glucanase (CMCase) activity increased by approximately 2.8- and 3-fold, while β -glucosidase ( p NPGase) activity increased by approximately 1.9- and 2.1-fold, respectively, compared with the activity in the WT strains. Furthermore, overexpression of NAD + synthesis pathways was found to elicit cellulase production by increasing the intracellular Ca 2+ concentration. In summary, this study is the first to demonstrate that increased intracellular NAD + contents through overexpression of the nmnat gene of NAD + synthesis pathways may increase cellulase production by increasing intracellular Ca 2+ concentrations in G. lucidum . Key points
The concentration of NAD
+
influences cellulase production in G. lucidum.
The concentration of NAD
+
influences the intracellular Ca
2+
concentration in G. lucidum.
The concentration of NAD
+
influences cellulase production by eliciting a change in intracellular Ca
2+
in G. lucidum.
研究领域:
微生物学
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生物技术
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酶学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 9
·2738
·
IF 4.412
Xiang Lai,Yu Han,Jie Zhang,Jinyu Zhang,Weifeng Lin,Zhiwei Liu,Longgang Wang
The sensitive and selective detection of dopamine (DA) is very important for the early diagnosis of DA-related diseases. In this study, we reported the colorimetric detection of DA usingGanoderma lucidumpolysaccharide (GLP) stabilized platinum nanoclusters (Ptn-GLP NCs). When Pt600-GLP NCs was added, 3,3’,5,5’-tetramethylbenzidine (TMB) was rapidly catalyzed and oxidized to blue oxTMB, indicating the peroxidase-like activity of Pt600-GLP NCs. The catalytic reaction on the substrate TMB followed the Michaelis-Menton kinetics with the ping-pong mechanism. The mechanism of the colorimetric reaction was mainly due to the formation of hydroxyl radical (•OH). Furthermore, the catalytic reaction of Pt600-GLP NCs was used in the colorimetric detection of DA. The linear range for DA was 1–100 μM and the detection limit was 0.66 μM. The sensitive detection of DA using Pt-GLP NCs with peroxidase-like activity offers a simple and practical method that may have great potential applications in the biotechnology field.Keywords:dopamine;detection;platinum;colorimetric;Ganoderma lucidumpolysaccharide
研究领域:
生物技术
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Weiwei Zheng,Suqing Lan,Weixi Zhang,Bingqian Nie,Kai Zhu,Xingqian Ye,Zhiqiang Hou,Shiguo Chen
The polysaccharides and triterpenes are important functional components of Ganoderma lucidum , but traditional preparation process of G. lucidum functional components can only realize the preparation of single functional component, which has poor targeting and low efficiency. In this study, the existence state of the functional components of G. lucidum was revealed. Then, the single step extraction process for functional components was established, and the precise structure evaluation of polysaccharide and triterpenes was conducted based on the process. The results showed that preparation time required for this strategy is only one-sixth of the traditional one, and 50 % of raw materials can be saved. Structural analysis of the functional components revealed that triterpenes were mainly Ganoderic acid and Lucidenic acid, and the polysaccharide structure was mainly 1,3-glucan and 1,3,6-glucan. The establishment of single step extraction strategy and the evaluation of the fine structure of functional components improved the efficiency of preparation and result determination, and provided an important basis for the development and utilization of green and low-carbon G. lucidum and even edible fungi resources and human nutritional dietary improvement strategies.
研究领域:
植物化学
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食品科学
·
药用真菌学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELL TRANSPLANTATION
·
2020
·
IF 3.341
Jing hui Bai,Jian Xu,Jian Zhao,Rui Zhang
Ganoderma lucidum is a popular traditional Chinese medicine used in China to improve health. Previous researches have revealed that the polysaccharide from G. lucidum could exert diversity activities, including immunomodulation, antioxidant, and antitumor effects. However, the effect of enzymatically hydrolyzed G. lucidum polysaccharide (EGLP) in colorectal cancer (CRC) progression remains unknown. The present research aimed to investigate the antitumor mechanism of EGLP in human colon cancer cells. For this purpose, the cytotoxic effects of EGLP were measured by the (3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) method. The apoptosis was evoked upon EGLP treatment, which was assayed using flow cytometry. The results indicated that EGLP may induce apoptosis in human colon cancer cell (HCT-116) cells via the upregulation of BCL-2 associated X protein (Bax), phospho-extracellular regulated protein kinases (P-ERK), and cleaved caspase-3 expression and downregulation of B-cell lymphoma-2 (Bcl-2), phospho-serine/threonine kinase 1 (p-Akt1), and cyclo-oxygen-ase (COX-2) expression. The obtained findings indicated EGLP as a new therapeutic agent in fighting CRC.
研究领域:
肿瘤学
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药理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Food Science and Human Wellness
·
2021
·
IF 5.154
Feiya Sheng,Songsong Wang,Xiao Luo,Jianbo Xiao,Linfeng Hu,Peng Li
Ganoderma (lingzhi) is a famous herbal medicine and edible supplement in oriental countries for a long history. In this study, a simple micellar electrokinetic chromatography (MEKC) method was established for the analysis of nucleosides and bases, the major bioactive components in Ganoderma for the first time. By optimizing the borate concentration, the sodium dodecyl sulfate (SDS) concentration and the pH value of running buffer, 10 nucleosides and bases achieved an ideal separation. In real sample analysis, the developed method was successfully used to determine the 10 target analytes in 23 batches of Ganoderma samples from different regions. Results indicated that contents of 10 investigated analytes in each sample showed obvious variation. The principal components analysis (PCA) and hierarchical cluster analysis (HCA) analysis classified the samples into three groups, and the HCA tree visualized the relationships which was mainly contributed by geographical partition. The results indicated geographical origin to be an important factor that affect the accumulation of nucleosides and bases in Ganoderma . In summary, this study provides a simple and practical strategy for quality assessment and cultivation reference of Ganoderma .
研究领域:
中药学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FITOTERAPIA
·
2022
·
IF 3.204
Da-Shuang Fang,Chun-Ru Cheng,Ming-Hua Qiu,Xing-Rong Peng
Three new meroterpenoids, cochlearins J–L ( 1 – 3 ) and three known meroterpenoids ( 4 – 6 ) were isolated from the fruiting bodies of Ganoderma cochlear . NMR ( 1 H and 13 C NMR, 1 H − 1 H COSY, HSQC, HMBC and ROESY), and HRESIMS were employed for the structure elucidation of new compounds. The stereostructures of 1 – 3 were confirmed by calculated ECD and optical rotation methods. Furthermore, compounds (+)- 1 , (−)- 1 , (+)- 2 , (−)- 2 , (+)- 3 , (−)- 3 , and 4 – 6 were evaluated for their α -glucosidase inhibitory activity. The results showed that compounds (+)- 1 , (−)- 1 and (+)- 2 exhibited stronger inhibition against α -glucosidase with IC 50 values of 24.18 ± 1.98, 26.49 ± 3.20 and 29.68 ± 2.73 μM, respectively, compared to the positive control ursolic acid (49.65 ± 2.21 μM). The molecular docking experiments reveal that (+)- 2 and (−)- 2 had different binding mode with α -glucosidase, leading to their different inhibition.
研究领域:
药用植物学
·
有机化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 21
·14506-14516
·
IF 3.9
Baoying Wang,Jingming Lan,Junjie Ou,Chunmiao Bo,Bolin Gong
Ganoderma lucidum bran (GB) has a broad application prospect in the preparation of activated carbon, livestock feed, and biogas, but the preparation of carbon dots (CDs) from GB has never been reported. In this work, GB was applied as a carbon source and nitrogen source to prepare both blue fluorescent CDs (BCDs) and green fluorescent CDs (GCDs). The former were prepared at 160 °C for 4 h by a hydrothermal approach, while the latter were acquired at 25 °C for 24 h by chemical oxidation. Two kinds of as-synthesized CDs exhibited unique excitation-dependent fluorescence behavior and high fluorescent chemical stability. Based on the fantastic optical behavior of the CDs, they were utilized as probes for fluorescent determination of copper ions (Cu2+). In the range of 1–10 μmol L−1, the fluorescent intensity of BCDs and GCDs decreased linearly with the increase of Cu2+ concentration; the linear correlation coefficient reached 0.9951 and 0.9982, and the limit of detection (LOD) was 0.74 and 1.08 μmol L−1, respectively. In addition, these CDs remained stable in 0.001–0.1 mmol L−1 salt solutions; BCDs were more stable in the neutral pH range, but GCDs were more stable in neutral to alkaline conditions. The CDs prepared from GB are not only simple and low-cost, but also can realize the comprehensive utilization of biomass.
研究领域:
材料科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Science and Technology
·
2022
·
IF 2.602
WANG Qingfu,HUANG Qinghua,ZHANG Liulian,WANG Lining,HU Biao,XU Riyi,LIANG Lei,PING Zhaohua
A novel sugarcane juice beverage was successfully produced by liquid fermentation of G. lucidum and homogenate process with selecting the day 10 fermentation broth as the raw materials. The beverage was evaluated for its sensory, nutritional and antioxidant activities. The results showed that the beverage by the G.lucidum fermentation was rich in polysaccharides, triterpenes, proteins, and flavonoids, which were all significantly higher than sugarcane juice, especially polysaccharides and triterpenoids. And the beverage could retain the sensory quality of sugarcane juice and could therefore be favored by the consumers. The in vitro antioxidant activity showed that the beverage had high scavenging and reducing abilities. DPPH and ABTS•+ radical scavenging rate of 1 mL fermented beverage were similar to 0.04 mg/mL VC, while the hydroxyl radical scavenging rate and reducing power of 1 mL of fermented beverage were similar to 1.68 mg/mL and 0.19 mg/mL VC, respectively. Taken together, the sugarcane juice fermented beverage was a natural flavor substances with high antioxidant activity and non-toxicity.
研究领域:
食品科学
·
微生物学
✓ 已验证
DOI
高影响因子
Journal of Analytical Methods in Chemistry
·
2018
·
IF 1.262
Hui Zhang,Huijie Jiang,Xiaojing Zhang,Shengqiang Tong,Jizhong Yan
Triterpenoids are the major secondary metabolites and active substances in Ganoderma, considered as the “marker compounds” for the chemical evaluation or standardization of Ganoderma. A response surface methodology was used to optimize the ultrasonic-assisted extraction of triterpenoids. The extraction rate was 7.338 ± 0.150 mg/g under the optimum conditions: 87% ethanol, ratio of solid to liquid (w : v) 1 : 28, and ultrasound extraction time 36 min. Based on the high sensitivity and selectivity of HPLC-LTQ-Orbitrap-MSn, 24 components of triterpenoids were tentatively identified in the negative mode. Then, the global chemical profiling consisting of HPLC and TLC fingerprints generated by ChemPattern™ software was developed for evaluation of Ganoderma species. For fingerprint analysis, 11 peaks of triterpenoids were selected as the characteristic peaks to evaluate the similarities of different samples. The correlation coefficients of similarity were greater than 0.830. The cluster analysis showed a clear separation of three groups, and 11 peaks played key roles in differentiating these samples. The developed global chemical profiling method could be applied for rapid evaluation, quality control, and authenticity identification of Ganoderma and other herbal medicines.
研究领域:
药用植物学
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）