BMC PLANT BIOLOGY
·
2019
·Vol. 19
·期 1
·1-15
·
IF 3.67
Li Taotao,Wu Qixian,Zhu Hong,Zhou Yijie,Jiang Yueming,Gao Huijun,Yun Ze
Background Banana anthracnose, caused by Colletotrichum musae, is one of the most severe postharvest diseases in banana. Melatonin is widely known for its role in enhancing plant stress tolerance. However, little is known about the control of melatonin on anthracnose in postharvest banana fruit.Results In this study, exogenous melatonin treatment could significantly reduce the incidence of anthracnose in ripe yellow banana fruit and delay fruit senescence. However, melatonin treatment did not affect the growth of Colletotrichum musae in vitro. Transcriptomic analysis of banana peel showed that 339 genes were up-regulated and 241 were down-regulated in the peel after melatonin treatment, compared with the control. Based on GO terms and KEGG pathway, these up-regulated genes were mainly categorized into signal transduction, cell wall formation, secondary metabolism, volatile compounds synthesis and response to stress, which might be related to the anti-anthracnose of banana fruit induced by melatonin treatment. This view was also supported by the increase of volatile compounds, cell wall components and IAA content in the melatonin-treated fruit peel via the metabolomic analysis. After melatonin treatment, auxin, ethylene and mitogen-activated protein kinase (MAPK) signaling pathways were enhanced, which might be involved in the enhanced fruit resistance by regulating physiological characteristics, disease-resistant proteins and metabolites.Conclusion sOur results provide a better understanding of the molecular processes in melatonin treatment delaying banana fruit senescence and anthracnose incidence.
研究领域:
植物病理学
·
分子生物学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 5
·2701
·
IF 4.9
Shizi Zhang,Yunfeng Xu,Fang Wang,Liyun Yang,Lijuan Luo,Lingyan Jiang
Anthracnose caused byColletotrichum gloeosporioidesis a destructive disease ofStylosanthes(stylo). Combination treatment of phloretin and pterostilbene (PP) has been previously shown to effectively inhibit the conidial germination and mycelial growth ofC. gloeosporioidesin vitro. In this study, the effects of PP treatment on the growth ofC. gloeosporioidesin vivo and the biocontrol mechanisms were investigated. We found that exogenous PP treatment could limit the growth ofC. gloeosporioidesand alleviate the damage of anthracnose in stylo. Comparative transcriptome analysis revealed that 565 genes were up-regulated and 239 genes were down-regulated upon PP treatment during the infection byC. gloeosporioides. The differentially expressed genes were mainly related to oxidative stress and chloroplast organization. Further physiological analysis revealed that application of PP afterC. gloeosporioidesinoculation significantly reduced the accumulation of O2•−level and increased the accumulation of antioxidants (glutathione, ascorbic acid and flavonoids) as well as the enzyme activity of total antioxidant capacity, superoxide dismutase, catalase, glutathione reductase, peroxidase and ascorbate peroxidase. PP also reduced the decline of chlorophyll a + b and increased the content of carotenoid in response toC. gloeosporioidesinfection. These results suggest that PP treatment alleviates anthracnose by improving antioxidant capacity and reducing the damage of chloroplasts, providing insights into the biocontrol mechanisms of PP on the stylo against anthracnose.Keywords:phloretin;pterostilbene;ROS scavenging;chloroplasts;anthracnose;stylo
研究领域:
植物病理学
·
分子生物学
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植物生理学
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DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Huifang Wu,Qiqi Wang,Lingfeng Zhu,Haoliang Zhang,Lizhong Liu
Anthrax, a fatal infectious disease caused by Bacillus anthracis ( B. anthracis ), poses a significant threat to public health. Herein, a novel type of carbon dots rich in π → π∗ transitions ( R -CDs) was developed to fabricate an R -CDs/Eu 3+ hybrid for the rapid on-site detection of the B. anthracis biomarker dipicolinic acid (DPA). The R -CDs/Eu 3+ hybrid exhibits two distinct fluorescence responses to DPA: (1) quenching of the R -CDs fluorescence due to the internal filter effect, and (2) enhancement of Eu 3+ fluorescence resulting from the antenna effect and the protective role of R -CDs, enabling ratiometric detection of DPA over a range of 0.1–100 μM with excellent selectivity. Furthermore, a portable, cost-effective device based on the hybrid was developed, enabling in-filed detection of DPA in lake water and real-time monitoring of DPA release from bacterial spores, which holds great promise for monitoring pathogenic biomarkers, with significant implications water safety and human health.
研究领域:
生物化学
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分析化学
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公共卫生
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 1
·400-410
·
IF 4.125
Siwei Wang,Xiaonan Wang,Qiang He,Haidan Lin,Hong Chang,Yanping Liu,Haibin Sun,Xiaobing Song
BACKGROUND Mango anthracnose is among the most severe diseases impacting mango yields and quality. While this disease can be effectively controlled through chemical means, it is vital that appropriate field efficacy and fate determination studies be conducted when applying pesticides to crops in order to appropriately gauge the ecological and health risks associated with the use of these agents. RESULTS GAP field trials were conducted to explore the efficacy, dissipation, and terminal residues associated with the application of mefentrifluconazole and pyraclostrobin to mango crops in six locations throughout China. These analyses revealed that three applications of mefentrifluconazole [160 mg active ingredient (a.i.) kg −1 ] in combination with pyraclostrobin mixture achieved satisfactory disease control efficacy. To simultaneously detect mefentrifluconazole and pyraclostrobin residues on mangoes, a ‘quick, easy, cheap, effective, rugged and safe’ (QuEChERS) high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS)-based approach was established. The initial mefentrifluconazole and pyraclostrobin concentrations ranged from 0.18 to 0.34 mg kg −1 , and these two compounds exhibited respective half-lives of 5.6 to 10.8 days and 5.5 to 9.0 days. At 21 days following foliage application, the terminal mefentrifluconazole and pyraclostrobin residue concentrations were 0.02–0.04 and 0.01–0.04 mg kg −1 , with these concentrations being below the maximum residue limit (MRL) established for pyraclostrobin. Both short-term [acute reference dose percent (ARfD%) 0.78–2.36% and 2.0–6.08%] and chronic [acceptable daily intake percent (ADI%) 0.08–0.47% and 0.09–0.55%] dietary intake risk assessments for mefentrifluconazole and pyraclostrobin indicated that these terminal residue concentrations are acceptable for the general population. CONCLUSION Mefentrifluconazole and pyraclostrobin in mango was rapidly degraded following first-order kinetics models. The dietary risk of mefentrifluconazole and pyraclostrobin through mango was negligible to consumers. The application of a 400 g L −1 mefentrifluconazole–pyraclostrobin suspension concentrate mixture represents a highly efficacious fungicidal approach to controlling mango anthracnose that exhibits significant potential for development as it is easily degraded and associated with low residual concentrations after application. © 2022 Society of Chemical Industry.
研究领域:
植物保护
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环境科学
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食品安全
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DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2019
·
IF 4.259
He Shengnan,An Tai,A Runa,Liu Shouan
The tea plant [Camellia sinensis (L.) O. Kuntze] is one of the most important leaf crops, and it is widely used for the production of non-alcoholic beverages worldwide. Tea also has a long history of medicinal use. Colletotrichum camelliae Massee is one of the dominant fungal pathogens that infects tea leaves and causes severe tea anthracnose disease. To analyze the molecular biology of C. camelliae, the quantification of pathogen gene expression by the RT-qPCR method is necessary. Reliable RT-qPCR results require the use of stable reference genes for data normalization. However, suitable reference genes have not been reported in C. camelliae thus far. In this study, 12 candidate genes (i.e., CcSPAC6B12.04c, CcWDR83, Cchp11, Ccnew1, CcHplo, CcRNF5, CcHpcob, CcfaeB-2, CcYER010C, CcRNM1, CcUP18, and CcACT) were isolated from C. camelliae and assessed as potential reference genes. The expression stability of these genes in C. camelliae during spore germination and mycelial growth and interaction with host plants was first evaluated using several statistical algorithms, such as geNorm, NormFinder, and Bestkeeper. A web-based analysis program, Refinder, was then used to find the most suitable reference genes. Our results indicated that Cenew1, CcHplo, and CcSPAC6B12.04c were the most stable reference genes in C. camelliae under all conditions. Our work provided the most suitable reference genes for future studies performed to quantify the target gene expression levels of C. camelliae.
研究领域:
植物病理学
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分子生物学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Xin-Yv Li,Yu-Jie Liu,Xian-Hua Meng,Chu Gong,Jun-Li Yang,Yin Qiang,Jun Wang,Xiu-Dan Hou
Anthracnose is a highly contagious and destructive plant disease caused by Colletotrichum gloeosporioides . The objective of this study was to investigate the antifungal activity and mechanism of ginsenoside Rk1 ( GRk1 ) against C. gloeosporioides. In vitro experiments demonstrated that GRk1 exhibited substantial antifungal activity, with EC 50 values of 64.58 mg L −1 , surpassing the plant-derived pesticide d -limonene . Additionally, GRk1 could effectively suppress the dissemination of anthracnose spots in postharvest fruit. By observing the microstructure of C. gloeosporioides , it was observed that the mycelium structure underwent notable changes, and autophagosomes engulfed the organelles in the experimental groups. Fluorescent staining revealed specific signals in mycelial autophagosomes, and Western Blot analysis confirmed the upregulation of the autophagy-related protein Atg8. These findings indicate that GRk1 may activate the autophagy process by promoting Atg8 expression. Proteomic analysis revealed that the expression of Sec20 and Mpv17 proteins was significantly downregulated, whose dysregulated expression is associated with the crosstalk between SNAREs and peroxisomes during vesicle trafficking and metabolic processes. Assessment of catalase (CAT) activity, hydrogen peroxide (H₂O₂) content, and superoxide anion (O₂ − ) levels further demonstrated that GRk1 could disrupt intracellular reactive oxygen species (ROS) homeostasis in mycelia. Moreover, the introduction of the ROS scavenger N -acetylcysteine (NAC) markedly inhibited autophagy, indicating that GRk1 promotes autophagy by inducing oxidative stress. Collectively, GRk1 inhibits pathogens via the ROS-autophagy axis. These findings suggested that GRk1 holds potential as a promising alternative to fungicides for the biological control of anthracnose.
研究领域:
植物病理学
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分子生物学
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真菌生理学
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采后生物学
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天然产物药理学
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产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2024
·
IF 6.4
Yujie Liu,Xinyv Li,Chu Gong,Yonghong Cao,Jun Wang,Min Han,Jun-Li Yang
Anthracnose is a plant disease caused by Colletotrichum spp ., known for its widespread infectivity and extreme destructiveness. Colletotrichum gloeosporioides is a representative pathogen of anthracnose in China. Gypenosides GP4 - GP7 , derived from Gynostemma pentaphyllum (Thunb.) Makino, could significantly inhibit the growth of C. gloeosporioides mycelial, with EC 50 values of 96.98, 27.5, 38.48, and 61.59 mg L −1 . The inhibitory effect of these compounds surpassed the commonly used chemical pesticide chlorothalonant and plant-derived pesticide matrine. Among them, the most active compound GP5 also showed a significant inhibitory effect on spore germination and bud tube elongation of C. gloeosporioides . In addition, GP5 could effectively suppress the spread of anthracnose spots in postharvest fruit. Transmission electron microscopy and fluorescence microscopy demonstrated that GP5 primarily exerted its antifungal function by activating cellular autophagy. Additionally, proteomics analysis revealed that GP5 had an antifungal effect against C. gloeosporioides by enhancing cellular autophagy through upregulation of the expression of the autophagy-related protein Atg8. This study presents a novel approach for the control and management of anthracnose in C. gloeosporioides . Consequently, GP5 has the potential to be developed as a plant-derived fungicide for the biological control of anthracnose.
研究领域:
植物病理学
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分子生物学
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植物化学
·
农学
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产品关联
DOI
高影响因子
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
·
2023
·
IF 2.7
Jiaoli Huang,Sisi Liu,Rukuan Liu,Youjin Yi,Changzhu Li,Zhihong Xiao,Jia Tu,Jingjing Xiao
Although Litsea cubeba essential oil (LCEO) is widely used for against fungi, antifungal mechanism of it is not fully understood. In the study, antifungal potential and mechanism of LCEO on Colletotrichum scovillei , the main pathogen fungi caused pepper anthracnose , were studied. Results of gas chromatograph-mass spectrometry (GC-MS) showed that citral (67.37%) and d -Limonene (11.72%) were the major components of LCEO. LCEO was effective in inhibiting germination of C. scovillei spores , and the minimum inhibitory concentration (MIC) was 0.60 mg mL −1 . To determine influence of LCEO on cell membrane integrity and permeability of C . scovillei , SEM observation, OD 260 nm (nucleic acids), OD 280 nm (proteins), extracellular electric conductivity , cell membrane permeability (propidium iodide, PI), cell wall integrity (calcofluor white, CFW), malondialdehyde (MDA) content and ergosterol content were analyzed. Spores exposed to LCEO were partly deformed, collapsed, shrunken and shriveled. The OD 260 nm, OD 280 nm and the extracellular electric conductivity of LCEO treatment group increased by 2.0, 1.5 and 1.2 times, respectively, at 12 h after treatment of LCEO (0.6 mg mL −1 ). Besides, high accumulation of MDA content indicated that LCEO significantly enhanced membrane lipid peroxidation . Moreover, LCEO induced a decrease in ergosterol content of C. scovillei . These findings suggest that LCEO exhibited potent inhibitory activities against C. scovillei , and the cell membrane permeability and cell wall integrity of C. scovillei treated by LCEO were significantly destroyed.
研究领域:
植物学
·
真菌学
·
农业科学
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DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Jie Zhong,Xin Yi Bai,Zhuo Zhang,Xiao Gang Li,Jun Zi Zhu
Colletotrichum scovillei is a pathogen that causes anthracnose in pepper fruit, resulting in severe postharvest losses in pepper fruit. Volatile organic compounds (VOCs) produced by microorganisms are regarded as promising environmentally safe fumigants for controlling postharvest diseases. In this study, the Streptomyces lactacystinicus ZZ-84 strain was identified as exhibiting strong antagonistic activity against C. scovillei and effectively controlling anthracnose in pepper fruit. The VOCs generated by ZZ-84 significantly inhibited the mycelial growth and conidial germination of C. scovillei , disrupted mycelial morphology, altered cell membrane permeability, and induced oxidative stress. Transcriptomic analysis revealed that the VOCs primarily affected the metabolic pathways of C. scovillei , particularly those related to protein synthesis, cell membrane integrity, redox homeostasis, and energy conversion. The volatiles 2-methyl-1-butanol, sulphuric acid dibutyl ester, and 6-methyl-2-heptanone were identified as the main antagonistic compounds among the VOCs and exhibited strong antifungal activity against C. scovillei. Overall, strain ZZ-84 has the potential to serve as a microbial fumigation agent for controlling postharvest anthracnose in pepper.
研究领域:
植物病理学
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微生物学
·
农业科学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Wei Ge,Linxuan Zhang,Fanli Meng,Chengming Tian
Poplar anthracnose caused by Colletotrichum gloeosporioides is one of the major forest diseases worldwide, causing incalculable losses in forestry production every year. In this study, an endophytic bacterium with significant antagonistic activity against C. gloeosporioides was isolated from Populus canadensis leaves, identified and named Pseudomonas atacamensis GZ-3. The specific biological functions of strain GZ-3 were analyzed using whole genome sequencing. The volatile organic compounds (VOCs) produced by strain GZ-3 had good antifungal activity against C. gloeosporioides . These VOCs could effectively inhibit mycelial growth and conidial germination of C. gloeosporioides . In addition, metabolomics analysis revealed that VOCs could help poplar resist infection of C. gloeosporioides by inducing the synthesis of flavonoids in poplar. 3-methyl-1-butanol and 2-heptanone were the main antifungal compounds in VOCs. Therefore, Pseudomonas atacamensis GZ-3 had good potential for biocontrol. This study provided a new strategy for the biological control of poplar anthracnose.
研究领域:
植物病理学
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微生物学
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森林保护学
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DOI
高影响因子
JOURNAL OF PHYTOPATHOLOGY
·
2022
·Vol. 170
·期 6
·373-381
·
IF 1.826
Ruofan Wei,Rong Wang,Yong Li,Moyi Yue,Wanlong Ding
Zhi mu ( Anemarrhena asphodeloides Bunge.) is an important medicinal plant in China, and its growth is greatly threatened by the fungal disease anthracnose, which can be caused by a number of species of Colletotrichum . Studying the phytopathogen and its sensitivities to fungicides will help to control anthracnose on zhi mu. Ten isolates of Colletotrichum were collected from zhi mu in Hebei, Anhui and Shanxi Provinces in China for this study. The isolates were identified as C . gloeosporioides , C. liriopes and C. spaethianum based on multi-locus phylogenetic analyses, including ACT , CHS - 1 , GADPH , HIS3 and ITS , micromorphological characteristics and the characteristics of colonies. Koch's postulates were confirmed by inoculating the leaves of zhi mu in vitro . The levels of virulence of 10 isolates varied significantly. Isolate YC-1 was the most virulent, while BZ-2 was the least virulent. The sensitivity of isolates YC-1, BY-1 and BY-2 ( C . spaethianum , C. gloeosporioides and C. liriopes respectively) to fungicides were examined. The EC 50 values of BY-1, BY-2, and YC-1 to tebuconazole, difenoconazole, prochloraz, fludioxonil, pyraclostrobin, carbendazim and azoxystrobin confirm that all of the isolates were markedly sensitive to the seven fungicides tested, strongly suggesting that these fungicides could be used to control anthracnose on zhi mu in the field.
研究领域:
植物病理学
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植物病原学
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药用植物
·
真菌病害
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2020
·Vol. 21
·期 5
·1828
·
IF 4.556
Songbo Lin,Yanan Dong,Xiwang Li,Yuxian Xing,Miaomiao Liu,Xiaoling Sun
Jasmonates (JAs), the group of lipid-derived hormones, were found to control the defense responses in a myriad of plants. Meaningfully, the macrolactones of 12-hydroxy jasmonate isoleucine (12OH-JA-Ile) were reported to induce the defensive response of wild tobacco. However, little to nothing has been known about the elicitation effect of JA-Ile-macrolactones on woody plants to harmful organisms, let alone its underlying mechanisms. Here, we first optimized the synthetic routine using mild toxic reagent isobutyl chloroformate instead of ethyl chloroformate for conjugation, and we used acetonitrile (MeCN) instead of ethyl alcohol for the better dissolution ofp-toluenesulfonic acid (p-TsOH) to gain JA-Ile-macrolactones. JA-Ile-macrolactone5b-treated tea plants significantly inhibited the larvae weight gain ofEctropis obliqualarvae and the lesions caused byColletotrichum camelliae. Furthermore, the expression level ofCsOPR3was significantly upregulated in5b-treated leaves. Meanwhile,5breduced the accumulation of eriodictyol 7-O-glucuronide (EDG) in tea plants, which was confirmed to promote the growth rate ofE. obliqualarvae by artificial diet assay. In conclusion, our study proved that the exogenous application of5bcould induce the tea plant resistance both to herbivoreE. obliquaand pathogenC. camelliae, and EDG was identified as one of the secondary metabolites that could influence the growth rate ofE. obliqua, but it did not directly influence the infection ofC. camelliaein vitro. Further research should be carried out to clarify the mechanism through which5binduces tea plant resistance toC. camelliae.Keywords:JA-Ile-macrolactone;induced resistance;Camellia sinensis;Ectropis obliqua;Colletotrichum camelliae
研究领域:
植物科学
·
分子生物学
·
昆虫学
·
病理学
✓ 已验证
DOI
PubMed
高影响因子
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