JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Kuo Liu,Yulin Wu,Yubo Ding,Yufeng Xiao,Lining Zheng,Donghui Liu,Hao Zhang
Nicosulfuron is a widely used sulfonylurea herbicide that generates persistent environmental residues that threaten ecosystems and human health. In this study, an efficient endophytic nicosulfuron-degrading bacterium, Serratia sp. NS8, was isolated from resistant barnyard grass and used to elucidate the molecular basis of nicosulfuron biodegradation. Strain NS8 removed 81.4% of 50 mg·L −1 nicosulfuron within 72 h, and the metabolite profiling revealed multiple degradation routes, including S–N bond cleavage and sulfonylurea bridge hydrolysis. Integrated multi-omics analyses demonstrated that strain NS8 mitigated oxidative injury through the activation of a glutathione-centered antioxidant system, along with the coordinated remodeling of membrane lipids and energy metabolism to generate a synergistic stress-response framework. Disruption of the bssS gene affected both motility and biofilm formation, leading to a corresponding reduction in degradation performance. Moreover, combined gene knockout, complementation, and western blot analyses established atzF as a central functional determinant of nicosulfuron biodegradation, with its deletion causing a 35.55% decline in the degradation efficiency, which was fully restored upon genetic complementation. The subsequent purification of the encoded allophanate hydrolase (AH) yielded a high-purity enzyme that was experimentally confirmed to catalyze the hydrolysis of nicosulfuron to 2-amino-4,6-dimethoxypyridine (ADMP). Collectively, these findings propose a synergistic degradation mechanism in NS8 encompassing antioxidant defense, enzymatic catalysis, and population-level regulatory behaviors, providing both mechanistic insight and a robust microbial candidate for the bioremediation of sulfonylurea herbicide contamination.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Ruiyu Fu,Tingze Ren,Yanhui Zhang,Xiaoli Guo,Xin Di
This paper describes a sensitive, simple and green analytical method for pretreatment of sulfonylurea and pyrethroid herbicides in water samples by supramolecular solvent-based liquid phase microextraction (SUPRAS-LPME). The SUPRAS was rapidly formed by nonanoic acid and tetrahydrofuran (THF)/water solution. The attractive features of SUPRAS-LPME include the simplicity, high enrichment factor, low cost and less consumption of organic solvents. The main influencing factors including type and volume of fatty acids, volume of THF , ionic strength, vortex time and volume of formic acid were optimized in detail, and then the significant variables were optimized by Box-Behnken design (BBD). Under the optimized condition, the proposed method shows high precision (RSDs < 13.7 %), low limits of detection (0.5–10 μg L -1 ) and limits of quantitation (10–45 μg L -1 ), acceptable recovery (71.3 %-95.9 %). The proposed method was successfully applied for analysis of sulfonylurea and pyrethroid herbicides in water samples, making it an alternative analytical strategy for determination of trace analytes at the microgram per liter range with desirable sensitivity.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Ting Zhao,Xiaochen Liang,Xinjie Guo,Xingjie Yang,Jialiang Guo,Xia Zhou,Xueqin Huang,Wenqian Zhang,Yiqing Wang,Zhaowei Liu,Zhengjin Jiang,Hongke Zhou,Haibo Zhou
A simple, sensitive method for pesticide distinguishment based on a colorimetric sensor array using diverse gold nanoparticles (AuNPs) at room temperature is presented in this study. Acetylcholinesterase (AChE) hydrolysis ability was influenced by different pesticides and produced different concentrations of thiocholine by hydrolyzing acetylthiocholine iodide (ATCh). Thiocholine could be easily linked to the AuNPs through an Au − S covalent bond, and AuNPs underwent aggregation, resulting in a visible color change due to alteration of surface plasmon resonance properties. Based on these results, we successfully distinguished eight pesticides (glyphosate, thiram, imidacloprid, tribenuron methyl, nicosulfuron, thifensulfuron methyl, dichlorprop, and fenoprop) utilizing five different AuNPs by colorimetric assay. The limit of detection (LOD) of this visual method for all pesticides was less than 1.5 × 10 -7 M, which was more sensitive than the U.S. Environmental Protection Agency regulations specify (1.18 ∼ 3.91 × 10 -6 M). This method was further improved by combining a portable smartphone device with a color picking application using (color name AR) and RGB (red, green, blue) values. The method was successfully applied to pesticide residue distinguishment in real samples by linear discriminant analysis (LDA).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Jianan Liu,Jinjin Cheng,Chunli Zhou,Liya Ma,Xiaolong Chen,Yong Li,Xing Sun,Xiaolong Yan,Renhua Geng,Qun Wan,Xiangyang Yu
Food safety problems caused by pesticide residues have always been a concern for many people. In this study, we investigated the uptake, translocation and subcellular distribution of neonicotinoid insecticides, triazole fungicides, and sulfonylurea herbicides in rice plants ( Oryza sativa L.). The time-dependent uptake kinetics of the three categories of pesticides with different molecular structures fit a first-order one-compartment kinetic model. The neonicotinoids (log K ow −0.66–0.8) were mainly concentrated in the leaves, and the triazoles (log K ow 3.72–4.4) were mainly concentrated in the roots. Neonicotinoid pesticides in the roots were preferentially transported across the membrane through the symplastic pathway; triazole pesticides except for triadimefon and myclobutanil preferentially passed through the symplastic pathway; and sulfonylurea pesticides (log K ow 0.034–2.89) were first transported upward through the apoplastic pathway. In the roots, neonicotinoids, triazoles, and sulfonylurea herbicides were mainly concentrated in the soluble fractions, cell wall and apoplast fractions, respectively. In addition, there was a high positive correlation between the subcellular distribution of pesticides in the roots, stems and leaves. Molecular weight and log K ow jointly affected the enrichment of triazole pesticides in the roots, stems and leaves and the transfer from stems to leaves, while water solubility and log K ow commonly affected neonicotinoids. There was a correlation between pesticide absorption and the molecular structures of pesticides. To develop pesticides with strong uptake and transport capabilities, it is necessary to consider that the electronegativity of some atoms is stronger, the sum of the topological indices of heteroatoms can be large, and the van der Waals volume increases accordingly.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiaoli Guo,Tingze Ren,Jianchun Ji,Yan Yang,Xin Di
In this work, a combination of QuEChERS and dispersive micro-solid phase extraction (D-μ-SPE) based on dissolvable layered double hydroxide (LDH) has been established for preconcentration and enrichment of sulfonylurea herbicides (SUHs) in wolfberry samples. The QuEChERS was used for extraction and purification of SUHs, followed by D-μ-SPE for further enrichment of targeted analytes to obtain superior extraction performance. Dissolvable LDH nanosheets were used as absorbents, thus eliminating the elution step needed in traditional D-μ-SPE. The main influence experimental variables including pH of sample solution, amount of LDH, vortex time and volume of acidic solution were optimized in detail. Under the optimized conditions, the proposed method shows high precision (RSDs < 12 .7%), low limits of detection (0.01–0.5 ng/g) and limits of quantitation (0.1–2.0 ng/g), acceptable recovery (80.1%–97.1%), thus making it a good alternative analytical strategy for the determination of SUHs residues at the low nanogram per gram range with desirable sensitivity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2022
·Vol. 12
·期 1
·1-13
·
IF 4.996
Li Miaomiao,Li Qingqing,Yao Jun,Sunahara Geoffrey,Duran Robert,Zhang Qinghua,Ruan Zhiyong
The overuse of the herbicide nicosulfuron has become a global environmental concern. As a potential bioremediation technology, the microbial degradation of nicosulfuron shows much promise; however, the mechanism by which microorganisms respond to nicosulfuron exposure requires further study. An isolated soil-borne bacteria Pseudomonas nicosulfuronedens LAM1902 displaying nicosulfuron, chlorimuron-ethyl, and cinosulfuron degradabilities in the presence of glucose, was used to determine the transcriptional responses to nicosulfuron exposure. RNA-Seq results indicated that 1102 differentially expressed genes (DEGs) were up-regulated and 702 down-regulated under nicosulfuron stress. DEGs were significantly enriched in “ABC transporters”, “sulfur metabolism”, and “ribosome” pathways ( p ≤ 0.05). Several pathways (glycolysis and pentose phosphate pathways, a two-component regulation system, as well as in bacterial chemotaxis metabolisms) were affected by nicosulfuron exposure. Surprisingly, nicosulfuron exposure showed positive effects on the production of oxalic acid that is synthesized by genes encoding glycolate oxidase through the glyoxylate cycle pathway. The results suggest that P. nicosulfuronedens LAM1902 adopt acid metabolites production strategies in response to nicosulfuron, with concomitant nicosulfuron degradation. Data indicates that glucose metabolism is required during the degradation and adaptation of strain LAM1902 to nicosulfuron stress. The present studies provide a glimpse at the molecular response of microorganisms to sulfonylurea pesticide toxicity and a potential framework for future mechanistic studies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2022
·
IF 9.988
Qingyun Ma,Hao Tan,Jinlong Song,Miaomiao Li,Zhiye Wang,Rebecca E. Parales,Lin Li,Zhiyong Ruan
This study aims to investigate the effects of long-term nicosulfuron residue on an herbicide factory ecosystem. High-throughput sequencing was used to investigate the environmental microbial community structure and interactions. The results showed that the main contributor to the differences in the microbial community structure was the sample type, followed by oxygen content, pH and nicosulfuron residue concentration. Regardless of the presence or absence of nicosulfuron, soil, sludge , and sewage were dominated by groups of Bacteroidetes, Actinobacteria, and Proteobacteria. Long-term exposure to nicosulfuron increased alpha diversity of bacteria and archaea but significantly decreased the abundance of Bacteroidetes and Acidobateria compared to soils without nicosulfuron residue. A total of 81 possible nicosulfuron-degrading bacterial genera, e.g., Rhodococcus , Chryseobacterium , Thermomonas , Stenotrophomonas , and Bacillus , were isolated from the nicosulfuron factory environmental samples through culturomics. The co-occurrence network analysis indicated that the keystone taxa were Rhodococcus , Stenotrophomonas , Nitrospira , Terrimonas , and Nitrosomonadaceae_MND1. The strong ecological relationship between microorganisms with the same network module was related to anaerobic respiration, the carbon and nitrogen cycle , and the degradation of environmental contaminants. Synthetic community (SynCom), which provides an effective top-down approach for the critical degradation strains obtained, enhanced the degradation efficiency of nicosulfuron. The results indicated that Rhodococcus sp. was the key genus in the environment of long-term nicosulfuron exposure.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Qingyun Ma,Qingqing Li,Jie Wang,Rebecca E. Parales,Lin Li,Zhiyong Ruan
Herbicide mixtures are a new and effective agricultural strategy for managing suppress weed resistance and have been widely used in controlling weeding growth in maize fields. However, the potential ecotoxicological impact of these mixtures on the microbial community structure and function within various root-associated niches, remains inadequately understood. Here, the effects of nicosulfuron , mesotrione and atrazine on soil enzyme activity and microbial community structure and function were investigated when applied alone and in combination. The findings indicated that herbicide mixtures exhibit a prolonged half-life compared to single herbicides. Ecological niches are the major factor influencing the structure and functions of the microbial community, with the rhizosphere exhibiting a more intensive response to herbicide stress. Herbicides significantly inhibited the activities of soil functional enzymes, including dehydrogenase , urease and sucrose in the short-term. Single herbicide did not drastically influence the alpha or beta diversity of the soil bacterial community, but herbicide mixtures significantly increased the richness of the fungal community. Meanwhile, the key functional microbial populations, such as Pseudomonas and Enterobacteriaceae, were significantly altered by herbicide stress. Both individual and combined use of the three herbicides reduced the complexity and stability of the bacterial network but increased the interspecific cooperations of fungal community in the rhizosphere. Moreover, by quantification of residual herbicide concentrations in the soil, we showed that the degradation period of the herbicide mixture was longer than that of single herbicides. Herbicide mixtures increased the contents of NO 3 − -N and NH 4 + -N in the soil in the short-term. Overall, our study provided a comprehensive insight into the response of maize root-associated microbial communities to herbicide mixtures and facilitated the assessment of the ecological risks posed by herbicide mixtures to the agricultural environment from an agricultural sustainability perspective.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2020
·Vol. 77
·期 1
·177-183
·
IF 3.75
Yuan-Yuan Zhang,Shuang Gao,Minh-Tu Hoang,Zi-Wei Wang,Xin Ma,Yue Zhai,Na Li,Li-Xia Zhao,Ying Fu,Fei Ye
BACKGROUND Herbicide safeners mitigate crop damage without reducing herbicide efficacy. Here, the protective effects of phenoxyacetyl oxazolidine derivatives as potential safeners were evaluated with a view toward reducing injury caused by sulfonylurea herbicide nicosulfuron to sensitive maize varieties. RESULTS Growth indices demonstrated that the bioactivity of compound 9 ( N -phenoxyacety-2-methyl-2,4-diethyl-1,3-oxazolidine) was superior to that of R-28725 and all other compounds tested. Compound 9 induced endogenous glutathione and upregulated glutathione- S -transferase (GST) in maize. Thus, it could enhance maize tolerance to nicosulfuron. Compared with the untreated water control group, the maximum reaction rate of GST was increased by 37.62%, while the maximum velocity of GST was decreased by 61.93% after treatment with compound 9 . Acetolactate synthase relative activity was significantly enhanced in the case of treatment with compound 9 , indicating the excellent protective effects of compound 9 against nicosulfuron in maize. CONCLUSIONS The present work demonstrates that phenoxyacetyl oxazolidine derivatives are potentially efficacious as herbicide safeners and merit further investigation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 9
·1938-1948
·
IF 2.413
Liang Ting,Gao Lei,Qin Dongli,Chen Ligang
A method for determination of sulfonylurea herbicides (SUHs) in grain was developed by using mesoporous structured molecularly imprinted polymers (M-MIPs) as adsorbents of matrix solid-phase dispersion (MSPD) extraction, coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this method, M-MIPs were synthesized by selecting chlorimuron-ethyl as template molecule, cetyltrimethylammonium bromide as mesoporous structure directing agent, 3-aminopropyltriethoxysilane as functional monomer, and tetraethyl orthosilicate as crosslinker. Then, M-MIPs were characterized by diverse instrumental techniques, absorption characteristics of M-MIPs were studied, and the experimental conditions for the MSPD extraction were optimized. MSPD enabled extraction and clean-up steps to be completed simultaneously. The M-MIPs with mesoporous structure improved detection selectivity, increased adsorptive capacity, and accelerated adsorption rate. Under the best conditions, excellent linearity of SUHs was obtained in the range of 0.25–25 μg kg −1 , the determination limits of chlorimuron-ethyl, nicosulfuron, thifensulfuron-methyl and chlorsulfuron were 0.075, 0.069, 0.083, and 0.105 μg kg −1 , respectively. The relative standard deviations were less than 5.5%.
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2015
·Vol. 8
·期 5
·1003-1012
·
IF 1.821
Xiaoxiao You,Ligang Chen
A new strategy for the isolation and enrichment of sulfonylurea herbicides in grain samples was obtained by using magnetic molecularly imprinted polymers (MMIPs) using carbon nanotubes as the matrix, nicosulfuron as the template molecule, 3-aminopropyltriethoxysilane as the functional monomer, tetraethyl orthosilicate as the cross-linker and cetyl trimethylammonium bromide as the dispersant. The characteristics of the MMIPs were assessed by transmission electron microscopy, vibrating sample magnetometry, X-ray diffractometry, elemental analysis, the Brunauer–Emmett–Teller method and Fourier transform infrared spectroscopy. The binding characteristics of the imprinted materials were researched using isothermal adsorption experiments, kinetics adsorption experiments and selectivity experiments. The recoveries of four sulfonylurea herbicides were more than 80.0% and the detection limits were 0.0107, 0.0151, 0.0147 and 0.0123 μg g−1, respectively. RSDs of intra-day and inter-day precisions in the range of 0.7–2.4% and 5.3–8.4% were obtained, respectively. The results demonstrate that MMIPs are promising for the preconcentration, purification and analysis of sulfonylurea herbicides in grain samples.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2016
·
IF 4.836
Hui Zhang,Mengya Li,Jie Li,Guangli Wang,Feng Li,Dayong Xu,Yuan Liu,Minghua Xiong
A natural consortium, named L1, of Rhodococcus sp. JT-3 and Brevundimonas sp. JT-9 was obtained from quizalofop- p -ethyl (QE) polluted soil. The consortium was able to use QE as a sole carbon source for growth and degraded 100 mg L −1 of QE in 60 h. Strain JT-3 initiated the catabolism of QE to quizalofop acid (QA), which was used by strain JT-9 as carbon source for growth and to simultaneously feed strain JT-3. A novel esterase EstS-JT, which was responsible for the transformation of QE to QA and essential for the mineralization of QE by the consortium, was cloned from strain JT-3. EstS-JT showed low amino acid identity to other reported esterases from esterase family VIII and represents a new member of this family. The deduced amino acid sequence contained the esterase family VIII conserved motifs S-X-X-K, YSV and WAG. The purified recombinant EstS-JT displayed maximal esterase activity at 35 °C and pH 7.5. An inhibitor assay, site-directed mutagenesis and 3D modeling analysis revealed that S 64 , K 67 and Y 175 were essential for catalysis and probably comprised the catalytic center of EstS-JT. Additionally, EstS-JT had broad substrate specificity and was capable of hydrolyzing p -nitrophenyl esters (C 2 -C 8 ) and various AOPP herbicides.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）