Infection and Drug Resistance
·
2025
·
IF 2.9
Kaixuan Yuan,Benshun Tian,Annan Zhan,Zhuoxi Chen,Long Ye,Yong Ling,Suiling Liu,Xuejiao Bai,Yunhu Zhao
Purpose Inactivating filamentous fungi (molds) reduce toxin production and transmission. This study evaluated the efficacy of formic acid (FA) fumigation to identify optimal conditions and its effects on fungal morphology. We aim to develop an efficient inactivation protocol for molds and provide valuable insights into laboratory techniques for fungal inactivation.Methods Initially, 238 clinical isolates from 23 species of molds were evaluated for the inactivation efficacy of formic acid, formaldehyde, and peracetic acid. After identifying the optimal sterilizing agent, 188 isolates from 16 clinically significant species were tested. Four concentrations (30%, 50%, 70%, and 100%) and six-time intervals (4 to 14 hours) were tested to optimize fumigation conditions. Morphological changes were analyzed using Aspergillus fumigatus ATCC MYA-3626 as the standard strain.Results The inactivation rates of 238 molds fumigated with formic acid, formaldehyde, and peracetic acid for 24 hours were 99.16%, 89.08%, and 90.35% (P < 0.05), indicating that formic acid was more effective than formaldehyde and peracetic acid. Subsequently, 188 isolates from 16 species were fumigated with formic acid at concentrations of 30%, 50%, 70%, and 100% for 24 hours, resulting in inactivation rate of 73.9%, 98.4%, 100%, and 100% (P < 0.05). Inactivation rate increased significantly with longer exposure times: 51.1% at 4 hours, 80.9% at 6 hours, 89.4% at 8 hours, 94.7% at 10 hours, 100% at 12 hours, and 100% after 14 hours (P < 0.05). After fumigation, A. fumigatus ATCC MYA-3626 growth ceased. Electron microscopy revealed irregular folds and roughness on spore surfaces, as well as collapsed or dead spores.Conclusion Formic acid demonstrated superior inactivation efficacy compared to formaldehyde and peracetic acid. At a concentration of 70% and a fumigation duration of 12 hours, the inactivation of molds was most effective.
研究领域:
真菌学
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微生物学
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临床实验室技术
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真菌病理学
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产品关联
DOI
PubMed
高影响因子
Antibiotics-Basel
·
2025
·Vol. 14
·期 5
·499
·
IF 4.6
Liyang Wang,Junying He,Hanzhong Feng,Qian Li,Meirong Song,Haoran Gou,Yongxing He,Kui Zhu
Background:Fungal infections pose an increasingly predominant threat to human and animal health. Modified compounds derived from chemo-diverse natural products offer enhanced therapeutic efficacies and promising approaches to combat life-threatening fungal pathogens.Methods:We performed biosynthetic gene clusters analysis of 2,4-diacetylchloroglucoside (DAPG) in 4292 shotgun metagenomes samples from the healthy and diseased skin. Then, we assessed the antifungal activity of DAPG and the derivative 2,4-diproylphloroglucinol (DPPG) against pathogenic fungi by minimum inhibitory concentrations. The inhibitory effects of DPPG were measured using hyphal growth assay and spore germination assay. Concurrently, the mechanism of DPPG onAspergillus fumigatuswas investigated in membrane permeability and fluidity. The therapeutic efficacy was evaluated in aGalleria mellonellainfection model.Results:We observed a significantly higher abundance of bacteria harboring DAPG biosynthetic clusters on healthy skin compared to diseased skin. Further, we designed and synthesized a series of phloroglucinol derivatives based on DAPG and obtained an antifungal candidate DPPG. DPPG not only exhibited robust antifungal activity againstAspergillusspp. andCandidaspp. but also impaired hyphal growth and spore germination ofA. fumigatusin vitro. A mechanism study showed that DPPG reduced membrane fluidity and increased the leakage of cellular contents, resulting in membrane perturbation and fungal death. Lastly, the therapeutic efficacy of DPPG was confirmed in aG. mellonellainfection model.Conclusions:Our study demonstrates that DPPG is a potent scaffold to combat invasive fungal infections.
研究领域:
微生物学
·
药理学
·
感染病学
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产品关联
DOI
PubMed
高影响因子
CARBON
·
2025
·
IF 11.6
Menghui Chi,Lingwen Gu,Ke Liu,Jing Lin,Qian Wang,Bing Yu,Ziyi Wang,Xueyun Fu,Daohao Li,Guiqiu Zhao,Cui Li
The photocatalytic antifungal strategy has attracted much attention in the field of fungicidal therapy due to its efficient therapeutic efficacy and avoidance of drug resistance. However, exploring safe and efficient photocatalysts remains an urgent challenge. In this work, nitrogen-doped carbon dots (N-CDs) combined with titanium dioxide (TiO 2 ) nanomaterials were synthesized by a hydrothermal method to exert a photocatalytic antifungal effect under sunlight irradiation. Compared with carbon dots/TiO 2 without N-doped modification, employing N doping to fabricate N-CDs/TiO 2 photocatalyst could further markedly optimize the bandgap structure of TiO 2 , improve the absorption of the solar spectrum and accelerate the electron-hole pairs separation, thereby greatly enhancing the photocatalytic reaction and antifungal effect. Remarkably, in the photocatalytic antifungal reaction, N-CDs/TiO 2 produces more oxygen radicals such as •OH and •O 2 − to rapidly hinder the germination of Aspergillus fumigatus hyphae and the formation of fungal biofilm. Additionally, the incorporation of N-CDs and TiO 2 possesses the great biocompatibility. Overall, this study proves that employing N-CDs/TiO 2 nanomaterials can be a promising and efficient therapeutic method against fungal infections.
研究领域:
微生物学
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材料科学
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医学
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产品关联
DOI
高影响因子
TheScientificWorldJOURNAL
·
2013
·
IF 1.73
Jun Tian,Xiaobin Zeng,Hong Zeng,Zhaozhong Feng,Xiangmin Miao,Xue Peng
The antifungal efficacy of nerol (NEL) has been proved against Aspergillus flavus by using in vitro and in vivo tests. The mycelial growth of A. flavus was completely inhibited at concentrations of 0.8 μL/mL and 0.1 μL/mL NEL in the air at contact and vapor conditions, respectively. The NEL also had an evident inhibitory effect on spore germination in A. flavus along with NEL concentration as well as time-dependent kinetic inhibition. The NEL presented noticeable inhibition on dry mycelium weight and synthesis of aflatoxin B1 (AFB1) by A. flavus, totally restraining AFB1 production at 0.6 μL/mL. In real food system, the efficacy of the NEL on resistance to decay development in cherry tomatoes was investigated in vivo by exposing inoculated and control fruit groups to NEL vapor at different concentration. NEL vapors at 0.1 μL/mL air concentration significantly reduced artificially contaminated A. flavus and a broad spectrum of fungal microbiota. Results obtained from presented study showed that the NEL had a great antifungal activity and could be considered as a benefit and safe tool to control food spoilage.
研究领域:
微生物学
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食品安全
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植物化学
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DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2025
·
IF 4.5
Mengmin Liang,Qingwen Hu,Junhao Yu,Heng Zhang,Sijie Liu,Jiangrong Huang,Yi Sun
Background: Invasive fungal infections (IFIs) constitute a significant health challenge, particularly among immunocompromised individuals, characterized by a high prevalence and associated mortality rates. The synergistic administration of Baicalein (BE) with azole antifungal agents could potentially herald a novel therapeutic paradigm.Materials and methods: 54 Aspergillus strains and 23 strains of dematiaceous fungi were selected. The standard M38-A2 microbroth dilution method was used to test the minimum inhibitory concentration (MIC) and the fractional inhibitory concentration index (FICI) of fungi when BE combined with itraconazole (ITC), voriconazole (VRC), posaconazole (POS) and Isavuconazole (ISV).Results: BE shows synergistic effects with POS and ITC, with 89.61% and 25.97% of fungal strains. The BE/POS regimen exerted synergistic effects in 87.04% of Aspergillus and an impressive 95.65% of dematiaceous fungi. In comparison, the BE/ITC combination showed significantly lower synergy, affecting 33.33% of Aspergillus and a mere 8.70% of dematiaceous strains. Antagonistic interactions were sporadically observed with BE in combination with ITC, VRC, POS and ISV. Within the azole class, the BE/POS pairing stood out for its frequent synergistic activity, in contrast to the absence of such effects when BE was paired with VRC or ISV. Highlighting the potential of BE/POS as a notably effective antifungal strategy.Conclusion: In vitro, BE/POS combination emerged as the most effective antifungal strategy, exhibiting synergistic effects in the majority of Aspergillus and dematiaceous fungi strains, whereas BE/ITC showed significantly less synergy, and BE with VRC or ISV displayed no synergistic activity.
研究领域:
医学
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微生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2018
·
IF 2.829
Hanlin Ding,Xuegang Luo,Xiaonuo Zhang,Hao Yang
A novel alginate-immobilized Aspergillus niger microsphere (AAM) biosorbent was prepared and used for Th(IV) removal. Biosorption experiments indicated that the AAMs with an abundance of surface functional groups exhibited rapid complexation with Th(IV) and a superior biosorption performance (303.95 mg·g −1 ) at pH 6.0 and 40 °C. Multiple methods of characterizing the biosorbent revealed the successful immobilization of gap structures with increased surface area and multisorption sites (e.g., hydroxyl , carboxyl and protonated amine groups). A method that combined the superior Th(IV) biosorption ability along with inexpensive and eco-friendly microbe materials exhibited considerable potential as an immobilizing biosorbents for thorium preconcentrations . In addition, the biomaterial had good recyclability through immobilization and inactivation. The proposed biosorbent is effective for the removal of radionuclides from contaminated aqueous solutions.
研究领域:
环境科学
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微生物学
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DOI
高影响因子
Catalysts
·
2022
·Vol. 12
·期 9
·1037
·
IF 4.501
Jianfeng Mei,Xiang Chen,Pingya Wang,Yichun Wu,Yu Yi,Guoqing Ying
Taxifolin is known to have multiple biological functions. It has been widely used as a multifunctional food additive, and consequently, the global demand for taxifolin is increasing. The main method for taxifolin production is an extraction from larch wood, but the global resources of larch are limited. Astilbin, taxifolin-3-o-rhamnoside, is abundant in many plants and much more readily available, meaning taxifolin can be obtained by deglycosylation of astilbin. In this study, a fungal strain,Aspergillus fumigatusSQH4, was isolated from an enrichment culture ofSmilax glabrarhizome to achieve the deglycosylation reaction. A culture of SQH4, adjusted to pH 6.5, with 5 g/L astilbin achieved a yield of taxifolin of 91.3% after biotransformation for 14 h at 35 °C. These findings offer an alternative method for the production of taxifolin.Keywords:taxifolin;dihydroquercetin;astilbin;biotransformation;Aspergillus fumigatus
研究领域:
微生物学
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生物技术
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药理学
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2014
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IF 2.884
Chengli Hu,Na Wang,Weiwei Zhang,Sheng Zhang,Yanfa Meng,Xiaoqi Yu
The aim of this study was to improve the ability of Aspergillus terreus lipase to separate the racemic ketoprofen vinyl ester into individual enantiomers using hollow self-assembly alginate- graft -poly(ethylene glycol)/α-cyclodextrins (Alg- g -PEG/α-CD) spheres as enzyme immobilization carriers. The morphology and size of the Alg- g -PEG/α-CD particles were investigated by transmission electron microscopy (TEM) and were found to be nanoscale. To facilitate recycling, calcium alginate (CA) beads were developed to encapsulate Alg- g -PEG/α-CD particles, thereby producing Alg- g -PEG/α-CD/CA composite beads. The influence of buffer pH and enzyme concentration during immobilization was studied along with the biocatalyst's kinetic parameters. When the immobilized enzyme was under optimal conditions in the resolution reaction, maximal conversion (approximately 45.9%) and enantioselectivity (approximately 128.8) were obtained. The immobilized A. terreus lipase maintained excellent performance even after 20 reuses and retained nearly 100% of its original activity after 24 weeks of storage at 4 °C.
研究领域:
生物化学
·
酶工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Wei-Hua Wang,Chu-Ran Li,Xu-Jie Qin,Xiao-Qin Yang,Si-Da Xie,Qian Jiang,Li-Hua Zou,Ying-Jun Zhang,Guo-Lei Zhu,Ping Zhao
Eleven alkaloids (1–11) including seven new ones, 1–7, were isolated from the solid fermentation of Aspergillus fumigatus VDL36, an endophytic fungus isolated from the leaves of Vaccinium dunalianum Wight (Ericaceae), a perennial evergreen shrub distributed across the Southwest regions of China, Myanmar, and Vietnam. Their structures were elucidated on the basis of extensive spectroscopic methods. The isolates were evaluated for in vitro antifungal activities against five phytopathogenic fungi (Fusarium oxysporum, Coriolus versicolor, Fusarium solani, Botrytis cinerea, Fusarium graminearum). As a result, the new compounds fumigaclavine I (1), 13-ethoxycyclotryprostatin A (5), 13-dehydroxycyclotryprostatin A (6), and 12β-hydroxy-13-oxofumitremorgin C (7) exhibited antifungal activities with MIC values of 7.8–62.5 μg/mL which were comparable to the two positive controls ketoconazole (MIC = 7.8–31.25 μg/mL) and carbendazim (MIC = 1.95–7.8 μg/mL). Furthermore, compounds 1 and 5 demonstrated potent protective and curative effects against the tomato gray mold in vivo. Preliminary structure–activity relationships of the tested indole diketopiperazine alkaloids indicate that the introduction of a substituent group at position C-13 enhances their biological activities.
研究领域:
药理学
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植物化学
·
真菌学
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产品关联
DOI
PubMed
高影响因子
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
·
2025
·Vol. 66
·期 11
·19-19
·
IF 4.7
Yuchen Liu,Xue Tian,Cui Li,Jing Lin,Lina Zhang,Qian Wang,Hong Li,Yuqi Li,Xiangzhi Liu,Guiqiu Zhao
Purpose:The purpose of this study was to investigate the therapeutic effect of artesunate (ART) on fungal keratitis (FK).Methods:The antifungal properties of ART againstAspergillus fumigatus(A. fumigatus) were confirmed by minimum inhibitory concentration (MIC), biofilm formation inhibition test, propidium iodide fluorescence, and calcium fluorescence white test. The levels of HSP90, BrlA, AbaA, WetA, CnaA, and CrzA were detected by quantitative reverse transcription PCR (qRT-PCR). Cell counting kit 8 (CCK-8) was used to detect the cytotoxicity of ART on RAW 264.7 cells and human corneal epithelial cells (HCECs). Clinical corneal score, hematoxylin and eosin (H&E) staining, and corneal fungal plate counts were used to evaluate the therapeutic effect of ART on FK in mice. The qRT-PCR, ELISA, and Western blot were used to investigate the effect of ART on inflammatory mediator expression during fungal infection.Results:ART inhibited the growth ofA fumigatus, biofilm formation, and conidium adhesion in vitro, and destroyed fungal cell walls and cytomembrane. In vivo, ART effectively reduced corneal fungal load. ART could reduce the inflammation of the cornea by reducing the accumulation of inflammatory cells and down-regulating the expression of TNF-α, IL-1β, and IL-6. ART could activate the autophagy pathway to play an anti-inflammatory role in FK by increasing the expression of Beclin-1 and LC3B.Conclusions:ART inhibits fungal growth by inhibiting biofilm formation, destroying fungal cell walls and membranes, and reducing the expression of HSP90 and calcineurin-related factors, and activates the autophagy pathway to reduce the expression of TNF-α, IL-1β, and IL-6 in FK by upregulating the protein expression of Beclin-1 and LC3B. ART has therapeutic potential for FK.
研究领域:
医学真菌学
·
药理学
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免疫学
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微生物学
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眼科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Qingzhi Ding,Yuan Zheng,Yongqi Zhu,Huamin Yang,Lin Luo,Haile Ma,Xiaoran Li
The individual and combined inhibitory activities of propolis extract (PE) and Hermetia illucens larval proteins (Black soldier fly larvae proteins) (BSFLP) against Aspergillus niger were investigated. The antifungal effects of PE, BSFLP and Synergy of PE and BSFLP (SPB) against Aspergillus niger were determined by mycelial growth , spore germination rate and hyphal elongation. Membrane integrity was measured by assessing changes in extracellular proteins, reducing sugars, and electrical conductivity . Intracellular MDA , H 2 O 2 , ergosterol , and mannan contents were also evaluated. SEM was used to observe the morphology of the Aspergillus niger mycelium . Additionally, AutoDock Tools software and multi-ligand docking methods were used to study the synergistic interactions of ligand combinations on the receptor. The results showed that the combination of PE and BSFLP had excellent effects. During the treatment of antifungal agents, the membrane permeability changed and a large amount of content leaked, while control check (CK) remained almost unchanged. Ergosterol content was found to increase, while mannan content decreased significantly, leading to cell death. In addition, SPB can cause abnormal accumulation of MDA and H 2 O 2 , leading to oxidative stress in cells. SEM results showed that morphology of Aspergillus niger treated with SPB was severe wrinkling, distortion and even breakage. Multi-ligand docking results showed that the binding affinity of receptor 1KS5 to 14 complex ligands increased, as indicated by the decrease in docking energy values. These results provide scientific basis for further study on the application of SPB and development of effective antifungal products.
研究领域:
微生物学
·
食品科学
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药理学
✓ 已验证
产品关联
DOI
高影响因子
CURRENT MICROBIOLOGY
·
2025
·Vol. 82
·期 11
·1-15
·
IF 2.6
Shen Wei,Wang Meng,Han Ting,Yao Yan,Ying Xinyi,Cui Yuyue,Du Peng
Previous research has indicated that the exopolysaccharide galactosaminogalactan (GAG) from Aspergillus fumigatus plays a pivotal role in the pathogenesis of experimental invasive aspergillosis by promoting virulence. However, it remains unclear whether the exopolysaccharide is directly virulent to host cells. In this study, we established a co-incubation system using human THP-1-derived macrophages and GAG extracted from A. fumigatus ATCC 1022 to assess the capacity of GAG to induce necroptosis in THP-1 cells, as well as to elucidate the underlying mechanisms of this process. Our findings indicate that the proportion of N-acetylgalactosamine in the GAG fraction derived from strain ATCC 1022 was lower, while GAG demonstrated a significantly greater ability to induce the production of the pro-inflammatory cytokines TNF-α and IL-1β, which was accompanied by the induction of necroptosis. Western blotting analysis revealed a significant increase in the phosphorylation of RIP1, RIP3, and MLKL proteins in GAG-treated THP-1 cells, and RIP1-RIP3-MLKL signaling activation and GAG-induced caspase activity-independent cell death were effectively inhibited by the TNF-α receptor-1 (TNFR1) inhibitor R-7050, the neutralizing antibody against TNF-α or necroptosis inhibitor Necrostatin-1. It's worth noting that the expression of CLEC4E, the gene encoding the Mincle receptor in macrophages, was elevated in the co-incubation system, potentially enhancing TNF-α-mediated necroptotic cell death. In addition to the RIP1-RIP3-MLKL axis, BAX, cleaved PARP, and JNK were also activated following GAG treatment, warranting further investigation into their roles in the regulation of necroptosis. Our findings demonstrate that GAG produced by A. fumigatus conidia during germination triggers necroptotic cell death in human THP-1 cells via TNF-α-mediated RIP1-RIP3-MLKL signaling.
研究领域:
微生物学
·
免疫学
·
分子生物学
·
感染性疾病
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真菌病理学
·
细胞死亡机制
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）