APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2011
·Vol. 93
·期 1
·191-201
·
IF 3.28
Wang Bingnan,Yang Xiufen,Zeng Hongmei,Liu Hua,Zhou Tingting,Tan Beibei,Yuan Jingjing,Guo Lihua,Qiu Dewen
PevD1, a novel protein elicitor from the pathogenic cotton verticillium wilt fungus, Verticillium dahliae , induced a hypersensitive response in tobacco plants. In this paper, the elicitor was purified and analyzed using de novo sequencing. The protein-encoding pevD1 gene consists of a 468-bp open reading frame that produces a polypeptide of 155 amino acids, with a theoretical molecular weight of 16.23 kDa. The sequence of elicitor protein PevD1 was matched to the genomic sequence (GenBank accession no. ABJE 01000445.1) of a putative protein from V. dahliae strain vdls.17, but a function had not yet been reported. The pevD1 gene was expressed in Escherichia coli , and the recombinant protein was characterized for its ability to confer systemic acquired resistance to tobacco mosaic virus (TMV). Recombinant PevD1-treated plants exhibited enhanced systemic resistance compared to control, including a significant reduction in the number and size of TMV lesions on tobacco leaves. The elicitor protein-induced hydrogen peroxide production, extracellular-medium alkalization, callose deposition, phenolics metabolism, and lignin synthesis in tobacco. Our results demonstrate that elicitor-PevD1 triggers defense responses in intact tobacco plants.
研究领域:
植物病理学
·
分子生物学
·
蛋白质化学
✓ 已验证
DOI
PubMed
高影响因子
ACS Omega
·
2025
·
IF 4.3
Hongjing Yang,Zhipeng Zang,Zhen Wang,Kai Song,Yunzhen Gao,Jinbin Wei,Lu Han,Jiandong Zhang,Chen Liu
Starch and cellulose are pivotal determinants of tobacco quality. In this work, starch and cellulose in flue-cured tobacco leaf were extracted through a sequential extraction process. First, an aqueous solution of tobacco leaves was prepared, and starch was extracted by boiling the leaves in water. The precipitate was separated, and the cellulose within it was hydrolyzed using a sulfuric acid solution. Then, starch content was quantified by a colorimetric reaction with iodine solution, while cellulose content was determined by using anthrone as a chromogenic agent. The reaction conditions were optimized to ensure accuracy, and the recovery rate and relative standard deviation (RSD) were calculated under these optimum conditions. The recovery rates for starch and cellulose in the tobacco samples ranged from 90 to 105%, with the RSD of interday and intraday determination being less than 5%. These results demonstrate that the proposed method is both sensitive and reproducible, making it suitable for the rapid and precise quantification of starch and cellulose in tobacco samples.
研究领域:
植物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Waste and Biomass Valorization
·
2018
·Vol. 10
·期 11
·3343-3350
·
IF 1.874
Chen Cheng,Jiang Linfeng,Ma Guanfeng,Jin Dongxue,Zhao Lisha,Ouyang Xinping
Abstract Lignin removal from lignocellulosic materials plays a great role in the utilization cellulose with high efficiency. In order to improve the quality of reconstituted tobacco sheet, lignin existed in tobacco stem was removed by enzymatic hydrolysis with laccase enhanced by weak alkali treatment together with the addition of Tween 80 into laccase treatment system. It is found that lignin was partially removed by laccase. The treatment of tobacco stem with NaHCO 3 resulted in the dissolution of lignin, and hence increased the porosity and specific surface area of the pretreated tobacco stem. Consequently, laccase could access to the tobacco stem more completely. The surface activity and dispersion of Tween 80 improved laccase activity, solubilization of degraded products of lignin, and contact interface between laccase and tobacco stem. The synergistic action of laccase, weak alkali and Tween 80 improved the ratio of lignin removal significantly, reaching as high as 40.3%. Graphical Abstract
研究领域:
生物化学
·
植物学
✓ 已验证
DOI
高影响因子
Archives of Agronomy and Soil Science
·
2023
·
IF 2.4
Zhaoyi Li,Xianhui Yin,You Luo,Sen Yang
Modern agriculture has caused extensive soil pesticide contamination, which is a global concern. This study explored the potential of bamboo charcoal (BC) to alleviate quinclorac-induced stress in tobacco plants. A pot experiment was conducted to investigate the effects of BC and vinasse fertilizer (VF) on the growth and physiological parameters of tobacco plants subjected to quinclorac stress. At 30, 60, and 90 d after transplantation, BC and VF exhibited pronounced restorative effects on quinclorac-stressed tobacco plants. The treatments improved plant height, leaf length, and leaf width, and enhanced leaf quality and various biochemical indicators. Treatment with 500 g VF + 4% BC resulted in the lowest residual quinclorac levels in both the soil and leaves. Treatment with 500 g VF + 2% BC resulted in the most significant recovery of agronomic traits, whereas 500 g VF + 0.5% BC was the most effective in enhancing leaf quality. However, the effectiveness of BC decreased with time following application, and furthermore, quinclorac may be released from the BC. In conclusion, BC shows promise in alleviating the adverse effects of quinclorac stress in tobacco plants.
研究领域:
农业科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Waste and Biomass Valorization
·
2019
·Vol. 11
·期 11
·6369-6382
·
IF 2.358
Liang Miao,Lu Wenjie,Lei Ping,Wang Le,Wang Bing,Li Bin,Shen Yi,Zhang Ke
Abstract Hydrothermal carbonization (HTC) combined with pelletization is an efficient upgrading process to convert biomass into energy-dense pellets. Binders usually serve an important role in the quality of biofuel pellets. Here, hydrochar was produced from tobacco stem through HTC, and was pelletized in the presence of various binders. The effect of four kinds of binders (K 2 CO 3 , CaCO 3 , xanthan gum and carboxymethyl cellulose) on the basic fuel properties, mechanical strength, moisture adsorption and combustion characteristics was investigated. The results showed that K 2 CO 3 assisted pellet had decreased compressive strength, increased hygroscopicity and high combustion residues. The compressive strength of pellets increased with increasing amount CaCO 3 binder probably due to the hardening effect of enhanced adhesion attraction forces between adjacent particles. Meanwhile, the CaCO 3 assisted pellet exhibited the lowest moisture uptake behavior. In addition, the introduction of organic binders led to improved mechanical strength, especially for xanthan binder, which could be ascribed to the formation of solid bridges and enhancement of adhesion forces between particles. TGA results suggested that the binder-assisted pellets exhibited desirable combustion characteristics such as decreased ignition temperature and increased comprehensive combustibility index. Kinetic parameters of the combustion process of binder-assisted pellets were also determined by employing the model free isoconversional Kissinger–Akahira–Sunose method. This study demonstrated that HTC of tobacco stem combined with pelletization in the presence of appropriate binders provide an alternative for solid biofuel pellets production. Graphic abstract Hydrothermal carbonization (HTC) of tobacco stem combined with pelletization in the presence of appropriate binders provide an alternative for solid biofuel pellets production.
研究领域:
能源植物学
·
生物质能工程
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
·
2013
·Vol. 32
·期 6
·1420-1425
·
IF 2.618
Bing-Lin Zhang,Sheng-Hua Shang,Hai-Tao Zhang,Zahra Jabeen,Guo-Ping Zhang
The effect of sodium chloride (NaCl) on cadmium (Cd) uptake, translocation, and oxidative stress was investigated using 2 tobacco cultivars differing in Cd tolerance. The growth inhibition of the tobacco plants exposed to Cd toxicity was in part alleviated by moderate addition of NaCl in the culture solution. Cadmium concentration of shoots and roots in the 2 cultivars increased with increasing Cd levels in the solution and decreased with the addition of NaCl. The addition of NaCl could alleviate the oxidative stress caused by Cd toxicity, as reflected by reduced production of malondialdehyde and recovered or enhanced activities of antioxidative enzymes catalase and glutathione peroxidase. The results also showed that the enhancement of antioxidative enzyme activity by NaCl for the tobacco plants exposed to Cd stress is related to induced Ca signaling. Environ Toxicol Chem 2013;32:1420–1425. © 2013 SETAC
研究领域:
植物学
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Yan-Bo Luo,Xue Li,Xing-Yi Jiang,Bao-Dong Cai,Feng-Peng Zhu,Hong-Fei Zhang,Zai-Gen Chen,Yong-Qiang Pang,Yu-Qi Feng
In this study, magnetic graphene was used as modified quick, easy, cheap, effective, rugged and safe (QuEChERS) adsorbent for the determination of organochlorine pesticide (OCPs) residues in tobacco. To achieve the optimum conditions of modified QuEChERS procedure toward target analytes, several parameters affecting the clean-up efficiency including the amount of the adsorbent and clean-up time were investigated. Under the optimized conditions, a method for the determination of 26 OCPs residues in tobacco was established by coupling the modified QuEChERS procedure to on-line gel permeation chromatography–gas chromatography–tandem mass spectrometry (on-line GPC–GC–MS 2 ). The limits of detection of proposed method for 26 OCPs residues ranged from 0.01275 to 3.150 ng/g. And good linearities of the proposed method were obtained with coefficients of determination ( R 2 ) greater than 0.9985 for all target analytes. Good reproducibility of method was obtained as intra- and inter-day precisions, the relative standard deviations were less than 11.1 and 15.0%, respectively. The apparent recoveries were in the range of 64–126% at different concentrations for real samples. Compared with the reported methods for the determination of OCPs residues in tobacco, the proposed method has the advantages of simple to operate, low cost and high clean-up ability. Finally, the method was successfully applied to the analysis of OCPs residues in real samples.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2022
·
IF 4.05
Li Xiao,Zhao Qingqing,Han Ming,Zhang Ke,Guo Zhongya,Li Bin,Wang Wei,Wei Xuhe,Liang Miao
In the present study, the effects of pretreatments with different solvents (hydrochloric acid, sodium hydroxide, and ethanol) on the physicochemical structure and pyrolysis behavior of tobacco stem (TS) were investigated. Elemental compositions analysis showed that acid washing increased the carbon content and exhibited a high removal percentage for the inorganic species. SEM, BET, and FTIR analysis showed that the pretreatments exerted different effect on the surface and microstructure. Furthermore, the pyrolysis characteristics analysis indicated that solvent pretreatment increased the initial pyrolysis temperature due to the removal of unstable volatile components, especially for the acid and alkali washing samples. Meanwhile, hydrochloric acid pretreatment increased the maximum pyrolysis rate temperature of cellulose decomposition stage and decreased the char residues due to the large removal of inorganic species, while alkali treatment produced the opposite result. Pyrolysis kinetic analysis based on Coats-Redfern method showed that F1.5 chemical reaction model can be used to describe the pyrolysis stages of TS samples and a decreased activation energy was observed during the decomposition of cellulose stage for the acid and alkali washed samples. The released gaseous products during the pyrolysis process were also monitored by using TG-FTIR technique, and the gaseous release behavior was different from each other.
研究领域:
能源与燃料
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE RESEARCH
·
2022
·
IF 2.975
Mingyu Yang,Zechun Liu,Jianping Zhang,Xiaolan Zhu,Wei Xie,Hongqiao Lan,Yanjun Huang,Xin Ye,Jun Yang
Cellulose and pectin are the important components of tobacco ( Nicotiana tabacum L.) cell wall, which affect the formation of undesirable compounds. Their contents are closely related to the harmfulness of tobacco. But the simultaneous quantitative analysis of cellulose and pectin is hard to be achieved for traditional analytical methods. A solid-state 13 C cross-polarization by multiple contact periods (multiCP) NMR method was developed for the simultaneous quantification of cellulose and pectin in tobacco. The multiCP spectrum at optimal parameters agreed well with the direct polarization (DP) spectrum within one-thirtieth of the measurement time and provided satisfactory signal to noise ratio (SNR). After three simple procedures of sample preparation and spectra deconvolution, simultaneous quantification of cellulose and pectin extracted from tobacco was effectively achieved. Compared with the chemical method, this interesting method was rapid, practicable, and very promising, which provided the technical support for the simultaneous quantification of cell wall substances in biological sample.
研究领域:
植物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2024
·
IF 3.8
Xinyan Jin,Decai Meng,Lili Fu,Yang Zhao,Qi Zhang,Zhan Zhang,Xianzhong Yin,Qinpeng Shen,Shuang Wang,Yue Zhang,Le Wang,Ping Lei,Bin Li
Understanding the puff-by-puff delivery mechanisms of key components of heated tobacco products is critical to developing product designs. This study investigates the puff-by-puff release patterns of key components in Natural Smoke Cigarettes (NSCs), which are designed to deliver nicotine without combustion by reducing oxygen content, utilizing a 30-s puff interval, a 2-s puff duration, and a 55 mL puff volume to simulate realistic smoking conditions. By establishing models to analyze the variation of nicotine, glycerol, 1,2-propylene glycol (PG), and water in different functional sections of the cigarette under controlled smoking conditions. These sections include the tobacco, hollow, cooling, and filter sections, constituting the structure of NSCs. In addition, the model calculates the port-by-port release of the components in the flue gas aerosol and compares it with the measured value. The results showed that: The retention amount in the tobacco section showed a steep decline in the first three puffs, with an overall exponential decrease. The amounts in the other sections were consistent, increasing in the first two puffs and then stabilizing. The retention amount decreased linearly with each puff, with a similar pattern across sections. The release amount peaked at the fourth to fifth puffs and then stabilized. The retention amount in the tobacco section declined exponentially in the first three puffs. It peaked in the second to third puffs in other sections, then decreased with each subsequent puff. The retention amount in the tobacco section showed a significant decline in the first puff, stabilizing at around 4 mg. In other sections, it peaked at the first puff and then rapidly declined. These findings can inform the development of reduced-harm smoking products and contribute to a better understanding of the dynamics of smoke generation. Additionally, the study offers a reference for the puff-by-puff release stability of NSCs and the improvement of consumers’ sensory quality.
研究领域:
生物学
·
医学
·
公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Agronomy-Basel
·
2024
·Vol. 14
·期 4
·801
·
IF 3.3
Yingnan Lin,Guofeng Yu,Rongning Liang,Fanyu Kong,Dean Song
With the increasing concerns of human health and the ecological environment, tobacco stalks from the cigarette industry have been recognized as hazardous wastes requiring cautious treatment. However, there is still a lack of a simple and efficient route for full utilization of tobacco stalks. Herein, we attempted to convert tobacco stalk waste to value-added carbohydrates, bio-oil, and biochar through sequential hydrothermal and pyrolysis treatments. The results showed a high carbohydrate yield under the optimized condition using a microwave reaction system. The subsequent pyrolysis of residual solids at high temperatures could produce unexpected high-level aromatic chemicals including phenols and even benzenes—difficult to form without the facilitation of catalysts or salts. The obtained by-product biochar showed rapid absorption of tetracycline in 5 min and gradual introparticle diffusion from 30 to 240 min. The isotherm result had the characteristics of the Langmuir model, displaying homogeneous active sites on the biochar surface. Probably due to the hydrothermal pretreatment before pyrolysis, the obtained biochar exhibited a high adsorption capacity toward tetracycline without introducing the activation chemicals. These results illustrate that the proposed strategy may pave the way for dealing with tobacco wastes in the agricultural field.
研究领域:
环境科学
·
生物质能
·
废物利用
✓ 已验证
产品关联
DOI
高影响因子
REVIEWS IN ANALYTICAL CHEMISTRY
·
2024
·Vol. 43
·期 1
·
IF 3.6
Xiaoxi Si,Yu Tian,Shufang Xu,Chunbo Liu,Zhenjie Li,Ji Yang,Ying Guan,Yangzhou Xie,Xiaoqing Shi,Zhigang Xu,Zhihua Liu
Alkaloid enantiomers have different physiological and pharmacological effects, and different chiral compositions have a large impact on cigarette sensory and use safety. Studying the effects of different configurations of alkaloid enantiomers on cigarette sensory is important for the development of tobacco products, but the chiral separation of alkaloid enantiomers has become the core problem. Here, the application of a C18 column coupled with a polysaccharide-based chiral column to separate and detect four pairs of alkaloid enantiomers in tobacco and cigarette smoke of different brands was investigated. Results showed that the tandem technology of the two columns enabled the baseline separation of four pairs of alkaloid enantiomers. The resolution of enantiomers of alkaloids in different tobacco samples differed in content and ratio, showing different chiral distribution characteristics. According to the different distribution characteristics of alkaloid enantiomers, tobacco samples of different brands can be distinguished and real cigarettes can be identified.
研究领域:
分析化学
·
烟草科学
·
药物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）