ELECTRONIC JOURNAL OF BIOTECHNOLOGY
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2025
·
IF 2.5
Yi Li,Jiacheng Tang,Kaixiang Xin,Zongda Chen,Lele Zhao,Yifan Zhao,Yinbiao Xu,Pei Zhou,Yang Sun,Yupeng Liu,Hua Li
Background Nicotinic acid dehydrogenase possesses the capability to convert nicotinic acid into 6-hydroxynicotinic acid, a compound of significant research value as a pharmaceutical intermediate. The extraction of nicotinic acid dehydrogenase is primarily performed by strains. However, the enzyme activity of the strains reported currently is relatively low, and their potential to catalyze the production of 6-hydroxynicotinic acid is insufficient to meet industrial requirements. Results Due to the revealing properties of 6-hydroxynicotinic acid, this study proposes a technique for calculating the luminescence intensity of colonies, which is based on a fluorescence spectrometer. The developed method establishes a reliable linear relationship (88.2%) between the luminescence intensity and enzyme activity. Consequently, it has been employed to screen strains that produce nicotinate dehydrogenase. This screening approach allows for the evaluation of about 500 enzyme-producing strains daily, presenting an efficient strategy for screening. Conclusions Through this approach, a novel high enzyme activity strain producing nicotinic acid dehydrogenase, Pseudomonas poae have been obtained, which designated as HD530. After process optimization, it was utilized to produce 6-hydroxynicotinic acid, achieving a high yield of 155.45 g/L within 72 h, meeting the requirements for industrial production. The effectiveness and potential of this technique lie in its application for strain screening and improvement.
研究领域:
微生物学
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生物技术
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制药工程
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DOI
高影响因子
Molecular Catalysis
·
2021
·
IF 5.062
Yu-Ting Shang,Jiufu Qin,Jin-Song Gong,Zi-Kai Wang,Heng Li,Hui Li,Jin-Song Shi,Zheng-Hong Xu
A nicotinate dehydrogenase producing strain was isolated and identified to be Pseudomonas putida , which could bio-transform nicotinic acid into 6-hydroxynicotinic acid. The activity of nicotinate dehydrogenase towards nicotinic acid could reach up to 1.11 U/mL and the yield of 6-hydroxynicotinic acid accumulated to 176 g/L within 30 h with a space time yield of 1.36 g/(g•h). In order to further improve its catalytic potential, a mutant library was constructed through atmospheric and room temperature plasma (ARTP) and rapidly screened by a high-throughput screening strategy, which obtained a mutant strain mut-5 with activity of 2.68 U/mL. Via developing a nitrilase-nicotinate dehydrogenase enzymatic cascade system, 54.5 g/L 6-hydroxynicotinic acid could be obtained within 585 min with 3-cyanopyridine as the substrate, which would further reduce the production costs. The results indicated that the nicotinate dehydrogenase (separately or combining with nitrilase) could serve as a superior catalyst for 6-hydroxynicotinic acid production. To our knowledge, the nicotinate dehydrogenase activity reported in this study was the highest level in literature to date.
研究领域:
生物催化
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微生物学
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酶工程
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代谢工程
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产品关联
DOI
高影响因子
Cell Reports
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2025
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IF 6.9
Zeng Zhang,Quan Guo,Zhihan Yang,Yukai Sun,Shuaiming Jiang,Yangli He,Jiahe Li,Jiachao Zhang
Sarcopenia significantly diminishes quality of life and increases mortality risk in older adults. While the connection between the gut microbiome and muscle health is recognized, the underlying mechanisms are poorly understood. In this study, shotgun metagenomics revealed that Bifidobacterium adolescentis is notably depleted in individuals with sarcopenia, correlating with reduced muscle mass and function. This finding was validated in aged mice. Metabolomics analysis identified nicotinic acid as a key metabolite produced by B. adolescentis , linked to improvements in muscle mass and functionality in individuals with sarcopenia. Mechanistically, nicotinic acid restores nicotinamide adenine dinucleotide (NAD+) levels in muscle, inhibits the FoxO3/Atrogin-1/Murf-1 axis, and promotes satellite cell proliferation, reducing muscle atrophy. Additionally, NAD+ activation enhances the silent-information-regulator 1 (SIRT1)/peroxisome-proliferator-activated-receptor-γ-coactivator 1-alpha (PGC-1α) axis, stimulating mitochondrial biogenesis and promoting oxidative metabolism in slow-twitch fibers, ultimately improving muscle function. Our findings suggest that B. adolescentis -derived nicotinic acid could be a promising therapeutic strategy for individuals with sarcopenia.
研究领域:
老年医学
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微生物学
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代谢组学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Aquaculture Reports
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2023
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IF 3.7
Wenlong Wan,Jie Xu,Jiayuan Shi,Xin Zhang,Anran Wang,Xiaojing Dong,Shuyan Miao
A 60-day feeding trial was conducted to determine the effects of the interaction of dietary niacin and lipid on the growth, antioxidant capacity and lipid metabolism in male Eriocheir sinensis . A total 162 crabs (initial weight, 33.27 ± 0.84 g) were fed with six semi-purified diets containing L 6 N 0 (6% lipid and 0 mg/kg niacin), L 6 N 400 (6% lipid and 400 mg/kg niacin), L 6 N 800 (6% lipid and 800 mg/kg niacin), L 12 N 0 (12% lipid and 0 mg/kg niacin), L 12 N 400 (12% lipid and 400 mg/kg niacin), L 12 N 800 (12% lipid and 800 mg/kg niacin), respectively. Elevated levels of niacin and lipid improved growth performance of crabs, but there were no significant differences. Crabs fed with 400 mg/kg niacin diets had higher hepatopancreas index (HSI) and lipase (LPS) than that of the other groups. Niacin promoted the accumulation of lipid in the hepatopancreas, but excessive accumulation led to more oxidative stress in crabs. In conclusion, excess lipid and niacin enhanced the growth performance of male Chinese mitten crabs by promoting the accumulation of lipid in the body at the expense of impairing their immune capacity. Crabs fed with 6% lipid and 400 mg/kg niacin diet had an advantage in lipid metabolism, without a significant decrease in antioxidant capacity, achieving a balance in growth performance and immunity.
研究领域:
水产养殖
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营养学
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生物化学
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产品关联
DOI
高影响因子
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
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2025
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IF 2.5
Yikai Chen,Wen Ye,Wenxin Wang,Nan Miao,Yongqiang Sha,Tao Sun
Major depressive disorder (MDD) is characterized by anhedonia and cognitive deficits. In this study, we investigated the therapeutic effects of nicotinic acid (NA), a nicotinamide adenine dinucleotide + (NAD + ) precursor, in a mouse chronic restraint stress (CRS) model. Behavioral assessments revealed that NA ameliorated depressive-like behaviors, evidenced by restored sucrose preference and reduced immobility in forced swim and tail suspension tests, without affecting motor coordination in the rotarod test. Hippocampal transcriptomic analysis indicated that NA reversed stress-induced gene dysregulation, activated neuroprotective Wnt/β-catenin, cGMP–PKG, and cAMP pathways, and rescued lipid and porphyrin metabolism. In conclusion, NA likely exerts antidepressant effects by remodeling hippocampal gene networks to enhance synaptic plasticity and restore metabolic homeostasis. These findings highlight NA as a promising metabolic antidepressant and support the development of NAD + precursor–based potential therapies for mood disorders.
研究领域:
神经生物学
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精神病学
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分子药理学
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代谢组学
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转化医学
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表观遗传学
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神经内分泌学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
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2023
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IF 2
Peng Qiu,Zhaorui Song
A simple and rapid fluorescence polarization immunoassay (FPIA) based on polyclonal antibodies is reported for the first time for the determination of nicotinic acid. First, six nicotinic acid analogues were coupled with fluorescein isothiocyanate isomer I (FITC) to obtain tracers. The influences of tracer structure on the performance of FPIA as well as the structure-activity relationships were explored. Subsequently, the tracer concentration and antibody dilution ratio on the performance of FPIA were investigated. For the optimal conditions, the detection range of FPIA was from 1.1 to 145.6 μg mL−1 and its IC20 value as the limit of detection (LOD) was 1.1 μg mL−1. The cross-reactivity of antibodies with common vitamins was evaluated. The intra-assay recovery was from 92.0% to 101.2% in food and tablets, with inter-assay recoveries between 92.0% and 103.8%. The developed FPIA provided good sensitivity and reproducibility and therefore provided efficient and economic screening for nicotinic acid in food and tablets.
研究领域:
食品科学
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药学
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免疫学
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产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
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2024
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IF 9.7
Hong Liao,Baojun Feng,Wenjun Ying,Zhina Lian,Junhua Zhang
The productive separation and conversion of corn straw offers significant prospects for the economic viability of biorefineries centered on straw resources. In this work, a graded utilization method was proposed to produce xylo -oligosaccharides (XOS), ethanol and lignin from corn straw by nicotinic acid (NA) hydrolysis and water/pentanol pretreatment . A XOS yield of 52.6 % was achieved under optimized conditions of 100 mM NA, 170 °C and 30 min. The solid residue was directly treated with water/pentanol, achieving a lignin removal rate of 79.7 %, and the total XOS yield was improved to 62.6 %. The lignin recovered from pentanol had a high purity of 97.6 %, with high phenolic OH content. Simultaneous saccharification and fermentation of final residue resulted in an ethanol yield of 92.0 %, which yielded 55.3 g/L ethanol. Thus, NA hydrolysis and water/pentanol pretreatment provided an efficient, environmentally friendly approach to fractionate corn straw for the co-production of XOS, ethanol, and lignin.
研究领域:
生化工程
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生物技术
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可再生能源
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农业科学
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产品关联
DOI
PubMed
高影响因子
IONICS
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2022
·Vol. 28
·期 7
·3525-3536
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IF 2.961
Lu Jingling,Xu Cunying,Li Jianru,Wang Shuxian,Xiang Qinqin,Chen Xiao,Hua Yixin,Li Yan
The effects of nicotinic acid (NA) on the electrodeposition of Al-Mn alloy have been investigated in aluminum chloride-1-butyl-3-methylimidazolium ionic liquids (AlCl 3 -BMIC) containing 0.15 mol·L −1 MnCl 2 (AlCl 3 -BMIC-MnCl 2 ). The electrochemical behaviors of the AlCl 3 -BMIC-MnCl 2 electrolyte have been studied here using cyclic voltammetry and potentiodynamic polarization. The addition of NA obviously increased the nucleation overpotential of Al-Mn alloy and inhibited the reduction reaction of Al(III) and Mn(II). The resultant surface morphologies, composition, and roughness of the Al-Mn alloy coating were revealed by scanning electron microscopy (SEM), transmission electron microscope (TEM), and atomic force microscopy (AFM). It was found that these additives strongly affect the morphology, composition, roughness, and crystal structure of the Al-Mn alloy coating. With the addition of NA, the content of Mn decreased, and Al-Mn alloy coatings became more uniform and smoother. The crystal structure of Al-Mn coatings was also examined by X-ray diffraction (XRD), where it was found that the phase composition of the deposits depends on the quantities of additives. For electrolyte containing little or no NA additives, the resulting alloy is amorphous structure. With NA additives up to 6·mmol L −1 , the Al-Mn coating changed into a mixture phase of amorphous and crystalline structure.
研究领域:
材料科学
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电化学
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DOI
高影响因子
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
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2016
·Vol. 27
·期 6
·1007-1013
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IF 1.096
Liu Lijun,Wang Honghong,Gong Shuwen,Lu Jing,Zhang Qian
A novel 12-molybdophosphoric acid (HPM)-based complex (H2PI)2(H3O)[PMo12O40][Mo2O5(H2O)2(PI)2]·11H2O (PI-HPM) was prepared by modification with picolinic acid (HPI) and characterized by the methods of Fourier transform infrared (FTIR) spectroscopy, thermogravimetry (TG), X-ray powder and single crystal diffraction. The complex retained the classical Keggin structure of bulk HPM, there were some strong hydrogen bonds existing between the [PMo12O40]3- polyanion, the [Mo2O5(H2O)2(PI)2] coordination moiety, the protonated HPI and the lattice water molecules. Then PI-HPM was employed as heterogeneous catalyst for esterification reaction to evaluate its acid-catalytic activity. The complex exhibited high activity and good durability in reaction mixtures, indicating that it was a promising heterogeneous acid catalyst for esterification that including the conversion of oleic acid to oleates.
研究领域:
化学
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催化科学
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DOI
高影响因子
ENVIRONMENTAL POLLUTION
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2020
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IF 6.793
Ye Tian,Dianzhen Yu,Na Liu,Yan Tang,Zheng Yan,Aibo Wu
Mycotoxins are toxic fungal metabolites, contaminating cereal grains in field or during processing and storage periods. These environmental contaminants pose great threats to humans and animals’ health due to their toxic effects. Type A trichothecenes, fumonisins and fusaric acid (FA) are commonly detected mycotoxins produced by various Fusarium species. Trichoderma spp. are promising antagonists in agriculture for their activities against plant pathogens , and also regarded as potential candidates for bioremediation of environmental contaminants. Managing toxigenic fungi by antagonistic Trichoderma is regarded as a sustainable and eco-friendly strategy for mycotoxin control. However, the metabolic activities of Trichoderma on natural occurring mycotoxins were less investigated. Our current work comprehensively explored the activities of Trichoderma against type A trichothecenes, fumonisins and FA producing Fusarium species via co-culture competition and indirect volatile assays. Furthermore, we investigated metabolism of type A trichothecenes and FA in Trichoderma isolates. Results indicated that Trichoderma were capable of bio-transforming T-2 toxin, HT-2 toxin, diacetoxyscirpenol and neosolaniol into their glycosylated forms and one Trichoderma strain could bio transform FA into low toxic fusarinol. These findings proved that Trichoderma isolates could manage toxigenic Fusarium via direct competition and volatile-mediated indirect inhibition. In addition, these antagonists possess defensive systems against mycotoxins for self-protection, which enriches our understanding on the interaction mechanism of Trichoderma spp. on toxigenic fungus.
研究领域:
微生物学
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食品安全
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植物病理学
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DOI
PubMed
高影响因子
Catalysts
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2023
·Vol. 13
·期 2
·371
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IF 3.9
Xue-Jiao Liu,Bao-Di Ma,Xiao-Mei Wu,Yi Xu
A recombinantE. coli, expressing nitrilase fromAcidovorax facilis72W with dual-site expression plasmid pRSFduet (E. colipRSF-AfNit2), was constructed. It showed higher soluble expression of nitrilase than that in the pET21a plasmid. The recombinant nitrilase can efficiently catalyze the hydrolysis of 3-cyanopyridine to nicotinic acid. The whole cells ofE. colipRSF-AfNit2 were immobilized by using sodium alginate/glutaraldehyde/polyethylene imine as the best immobilized reagents. The immobilized cells showed 95% activity recovery and excellent mechanical strength, with improved thermal stability and pH stability. They also retained 82% of initial activity after nearly two months of storage at 4 °C. A semi-continuous packed-bed bioreactor (sPBR) filled with the immobilized cells was studied for efficient production of nicotinic acid. After optimization, the highest space–time yield of 1576 g/(L·d) was obtained on 0.8 M substrate concentration at 2 mL/min of flow rate. The sPBR was repeatedly operated for 41 batches, keeping 100% conversion in the presence of 30 mM CaCl2. Finally, 95 g of nicotinic acid were obtained at 90% yield after separation and purification. The developed technology has potential application value.Keywords:nitrilase;cell immobilization;nicotinic acid;reaction optimization;semi-continuous packed-bed bioreactor
研究领域:
生物技术
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生物化工
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微生物学
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产品关联
DOI
高影响因子
JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY
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2022
·Vol. 102
·期 7
·619-628
·
IF 1.925
Yu Zhi-chao,Lu Yun,Shan Pei-hui,Fan Ying,Tao Zhu,Xiao Xin,Wei Gang,Prior Timothy J.,Redshaw Carl
The complexation between cucurbit[8]uril, Q[8], and isonicotinic acid has been studied using 1 H NMR spectroscopy, UV–Vis absorption spectroscopy, Raman spectroscopy and single crystal X-ray diffraction. The results revealed that the 2:1 inclusion complex (4-PA) 2 @Q[8]·25H 2 O is formed, with two guests simultaneously encapsulated in the hydrophobic cavity; the mean planes of the guests are 3.535 Å apart. Graphical abstract
研究领域:
化学
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药物科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）