ADVANCED FUNCTIONAL MATERIALS
·
2023
·
IF 19
Ting Zhang,Yuting Li,Xiaoming Lv,Shen Jiang,Shuang Jiang,Zhongqi Sun,Mingxu Zhang,Yang Li
The detection of biomolecules utilizing surface-enhanced Raman spectroscopy (SERS) is highly trending in research, but the unlabeled and sensitive detection of saccharides in body fluids is challenging. Here, a novel SERS substrate (AgNS 600 ) is fabricated by physical scratching of smooth silver sheets, which successfully achieves enhanced SERS fingerprinting by capturing glucose molecules through nanoscale grooves on the surface of the substrate to form multi-dimensional “hot spot” regions. The platform proves to be rapid, reliable, and reproducible, with a lower detection limit of 0.5 amol L −1 in deionized water. Further detection of multiple monosaccharides, polysaccharides, and mix samples, which are distinguished with machine learning and heat map, demonstrates the versatility and specificity of the platform. Importantly, real samples are explored and the results show that the platform is able to detect and recognize SERS signals of glucose in the blood, urine, tears, and perspiration, and non-destructive predict diabetes by analyzing the signal intensity of different characteristic peaks. In this work, it is the first non-chemical, un labeled determination of glucose with high sensitivity via SERS, which provides a potential strategy for advancing the clinical development of SERS technology and the early diagnosis of glucose metabolic diseases.
研究领域:
生物医学工程
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分析化学
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分子诊断
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Xinbing Jiang,Shaochong Zhu,Huan Yang,Yuxi Fei,Jiuhong Wang,Shujiang Ding
Intracellular three-dimensional (3D) thermal mapping is a valuable technical tool for answering fundamental scientific questions about cellular thermal behavior and subcellular diagnostic therapy. The best method for achieving intracellular 3D thermal imaging is to collect intracellular temperature response while also obtaining a 3D position for the corresponding location. However, temperature measurement in cell space has not been accurately characterized due to the difficulty in accurately locating the 3D position in microregions. In this article, we developed a QD-based intracellular 3D thermal imaging system by combining QD fluorescence thermometry with double-helical point spread function (DH-PSF)-based axial localization. Liposome transfection was used to achieve the cellular uptake of CdTe/CdS/ZnS QDs in HOS cells. Five QDs’ temperatures with spatial distribution in living cells were realized in the axial range of 1.35 μm by collecting the thermosensitive fluorescence emission of discrete QDs and the axial imaging localization information, and their 3D thermal image distribution was mapped. Measurements and analysis show that the combination of QD fluorescence thermometry and axial localization of DH-PSF enables the acquisition of thermal image information in intracellular 3D microregions. Using this system, temperature variations at 3D locations in living cells exposed to ionomycin Ca2+ stress were also monitored. The method described in this article can be applied broadly to the study of intracellular space thermal response and thermal regulation.
研究领域:
生物物理学
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纳米医学
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细胞生物学
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产品关联
DOI
高影响因子
CARBON
·
2021
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IF 9.594
Cheng Peng,Qiang Li,Liang Niu,Hong Yuan,Jiahui Xu,QingQing Yang,YuanJun Yang,Guoxiang Li,Yanwu Zhu
The development of portable and wearable electronic devices continues to increase the demand for miniature energy storage units. Here, we fabricate the flexible micro-supercapacitors (MSCs) through direct heating pattern on graphene oxide film. The shape designable MSCs are based on planar reduced graphene oxide/graphene oxide (rGO/GO) structure, with rGO as electrode and GO as separator. The GO area heated by heating pen is reduced to rGO, and the area with no heating is still GO. The interdigitated MSC made at 400 °C delivers high area capacitance of 94.8 mF cm −2 at current density of 0.25 mA cm −2 within poly(vinyl alcohol) (PVA)/H 2 SO 4 gel electrolyte. It provides high energy density of 10.7 mWh cm −2 at power density of 112.6 mW cm −2 . In addition, it also has excellent mechanical stability, with basically unchanged area capacitance from 0° to 180° bending angles. The MSCs with shapes of spiral, concentric circle and eight-trigrams are also fabricated via this method. The results indicate that heating pattern on GO films to build flexible MSC is a promising and low-cost new method to fabricate energy storage equipment for future electronic devices.
研究领域:
材料科学
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能源存储技术
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产品关联
DOI
高影响因子
Journal of Translational Medicine
·
2022
·Vol. 20
·期 1
·1-14
·
IF 8.44
Mu Shuwen,Lin Yinghong,Xu Yongjun,Wei Xianqing,Zeng Zihuan,Lin Kunzhe,Zhu Linghua,Liu Qinghong,Qi Xingfeng,Wei Liangfeng,Liang Shengxiang,Wang Shousen
Background Cerebral venous sinus thrombosis (CVST) is a rare neurovascular disorder with highly variable manifestations and clinical courses. Animal models properly matched to the clinical form of CVST are necessary for elucidating the pathophysiology of the disease. In this study, we aimed to establish a rat model that accurately recapitulates the clinical features of CVST in human patients. Methods This study consisted of a clinical analysis and animal experiments. Clinical data for two centres obtained between January 2016 and May 2021 were collected and analysed retrospectively. In addition, a Sprague–Dawley rat model of CVST was established by inserting a water-swellable rubber device into the superior sagittal sinus, following which imaging, histological, haematological, and behavioural tests were used to investigate pathophysiological changes. Principal component analysis and hierarchical clustering heatmaps were used to evaluate the similarity between the animal models and human patients. Results The imaging results revealed the possibility of vasogenic oedema in animal models. Haematological analysis indicated an inflammatory and hypercoagulable state. These findings were mostly matched with the retrospective clinical data. Pathological and serological tests further revealed brain parenchymal damage related to CVST in animal models. Conclusions We successfully established a stable and reproducible rat model of CVST. The high similarity between clinical patients and animal models was verified via cluster analysis. This model may be useful for the study of CVST pathophysiology and potential therapies.
研究领域:
神经病学
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实验医学
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病理学
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生物统计学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Yamin Liu,Xunjiang Wang,Jiaojiao Wei,Kangning Fu,Yilin Chen,Linnan Li,Zhengtao Wang,Li Yang
The severe respiratory dysfunctions associated with acute lung injury (ALI) and its sequelae have a high morbidity and mortality rate, are multifactorial, and lack a viable treatment. Considering the critical function that amino acids and derivatives play in the genesis of illnesses and the regulation of metabolic processes, monitoring the levels of metabolites associated with amino acids in biological matrices is necessary and interesting to study their pathological mechanisms. Exploring the dynamics of amino acid and derivatives levels and searching for biomarkers provides improved clinical ideas for the diagnosis and treatment of ALI. Therefore, we developed an ultra-high-performance liquid chromatography-electrospray tandem mass spectrometry (UHPLC-MS/MS) method that can simultaneously determine the amino acid and derivatives metabolic levels to study amino acid profiles in different biological samples to facilitate clinical research of ALI. In this study, 48 amino acids and derivatives, including neurotransmitters, polyamines, purines, and other types, were quantified simultaneously in a fast, high-throughput, sensitive, and reliable manner within a 15-minute run time without derivatization. No relevant studies have been reported to quantify these 48 amino acid metabolites in three biological samples simultaneously. Satisfactory linearity (R > 0.995), inter-day and intra-day accuracy (85.17–112.67% and 85.29–111.60%, respectively), inter-day and intra-day precision (RSD < 13.80% and RSD < 12.01%, respectively), matrix effects (81.00%–118.00%), recovery (85.09%–114.65%) and stability (RSD < 14.72%) were all demonstrated by the optimized method's successful validation for all analytes. In addition, the suggested method was effectively implemented in plasma, urine, and lung tissue from normal mice and mice with ALI, with the aim of finding potential biomarkers associated with ALI. Potential biomarkers were screened through multivariate statistical analysis and volcanic map analysis, and the changes of markers in ALI were again identified through heat map analysis and correlation analysis with biochemical indicators, which provided ideas and references for subsequent mechanism studies. Here, the technique created in this work offers a quick and dependable way to perform an integrated analysis of amino acids in a variety of biological materials, which can provide research ideas for understanding the physiopathological state of various diseases.
研究领域:
肺病学
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分析化学
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代谢组学
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临床研究
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生物化学
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产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2024
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IF 20.2
Longde Jiang,Jingde Luan,Haowei Zhang,Yu Bai,Yu Zhang,Wengang Liu,Zheng Yan,Haiting Zhao
Photocatalytic H 2 production from seawater splitting is a promising method for sustainable energy conversion technology. The vanadium-oxygen binary doped carbon nitride (VO X -CN) is successfully prepared for H 2 production. Benefiting from the electronic structure alteration, the maximum H 2 yield on VO X -CN in artificial seawater reaches 4475.74 μmol h −1 g −1 , 10 times higher than that of CN in deionized water. The impact of ions on the photocatalytic activity of VO 2 -CN is followed by Na + > Mg 2+ > K + > Ca 2+ . The adsorption of electrolyte ions promotes the formation of delocalized electron clouds on the surface of VO 2 -CN, facilitating more electrons to participate in H + reduction. Screening based on six machine models, the decision tree regression algorithm is used to reveal the interaction between cation type, their concentration and H 2 yield. Pearson heatmap indicates that Na + , Mg 2+ , and K + ions are positively correlated with H 2 yield, whereas Ca 2+ shows a negative correlation. The interactions among Na + , Mg 2+ and K + atoms facilitate H 2 production in multi-ion system, whereas Ca 2+ ions do the opposite, especially for high concentration. This work proposes new insights into the interference on H 2 yield between the structure alteration of CN framework and the change of ion type and concentration.
研究领域:
材料科学
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催化
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能源转换
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化学工程
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机器学习
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yuan Pan,Hui-Wen Gu,Yi Lv,Xiao-Li Yin,Ying Chen,Wanjun Long,Haiyan Fu,Yuanbin She
Identifying geographical origins of red wines made in specific regions is of significance since the false claim of geographical origins has been frequently exposed in China's wine industry. In this work, an untargeted metabolomic approach based on UPLC-QTOF-MS was established to discriminate geographical origins of Chinese red wines. The principal component analysis (PCA) showed significant differences between wine samples from three famous geographical origins in China. The metabolites contributing to the differentiation were screened by orthogonal partial least squares-discriminant analysis (OPLS-DA) with pairwise modeling. 40 and 46 differential metabolites in positive and negative ionization modes were putatively identified as chemical markers. Furthermore, heatmap visualization and OPLS-DA models were constructed based on these identified markers and external verification wine samples from different regions were successfully discriminated, with recognition rate up to 96.7%. This study indicated that UPLC-QTOF-MS-based untargeted metabolomics has great potential for the geographical origin traceability of Chinese red wines.
研究领域:
食品科学
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分析化学
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代谢组学
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葡萄酒科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Ting Xiao,Junpeng Zeng,Caidong Zhao,Yanmei Hou,Tong Wu,Zeyuan Deng,Liufeng Zheng
An infant in vitro digestion model was utilized to investigate protein digestion characteristics in human and diverse mammalian milk (i.e., cow, goat, sheep, mare, and camel milk) using electrophoresis and chromatography. Digestive differences among milks were mainly manifested in the infant gastric phase, as evidenced by varying degrees of protein digestion. Notably, proteins (i.e., lactoferrin, serum albumin, and immunoglobulin G-heavy chain) remained partially intact in human milk, whereas these proteins in animal milk were exclusively degraded after gastrointestinal digestion. The peptide spectra of human, mare, and camel milk were highly similar, with a predominant formation of low-intensity small peptides, whereas the other three milk showed the opposite phenomenon. Heatmap cluster analysis indicated that camel milk was the most comparable to human milk before digestion, yet sheep milk was the most similar to human milk regarding protein digestion behaviors following infant gastric digestion.
研究领域:
营养学
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食品科学
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生物化学
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儿科
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 3
·602
·
IF 4.2
Yang Yue,Jiaxin Yin,Jingyi Xie,Shufang Wu,Hui Ding,Lifeng Han,Songtao Bie,Wen Song,Ying Zhang,Xinbo Song,Heshui Yu,Zheng Li
The flower buds of threePanaxspecies (PGF:P. ginseng; PQF:P. quinquefolius; PNF:P. notoginseng) widely consumed as health tea are easily confused in market circulation. We aimed to develop a green, fast, and easy analysis strategy to distinguish PGF, PQF, and PNF. In this work, fast gas chromatography electronic nose (fast GC e-nose), headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS), and headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) were utilized to comprehensively analyze the volatile organic components (VOCs) of three flowers. Meanwhile, a principal component analysis (PCA) and heatmap were applied to distinguish the VOCs identified in PGF, PQF, and PNF. A random forest (RF) analysis was used to screen key factors affecting the discrimination. As a result, 39, 68, and 78 VOCs were identified in three flowers using fast GC e-nose, HS-GC-IMS, and HS-SPME-GC-MS. Nine VOCs were selected as potential chemical markers based on a model of RF for distinguishing these three species. Conclusively, a complete VOC analysis strategy was created to provide a methodological reference for the rapid, simple, and environmentally friendly detection and identification of food products (tea, oil, honey, etc.) and herbs with flavor characteristics and to provide a basis for further specification of their quality and base sources.Keywords:Panax;flower bud;fast GC e-nose;HS-GC-IMS;HS-SPME-GC-MS;multivariate statistical analysis
研究领域:
分析化学
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植物化学
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食品科学
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药用植物学
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产品关联
DOI
PubMed
高影响因子
ACS Sensors
·
2021
·
IF 7.711
Xinwei Wei,Deming Jiang,Changming Chen,Jianguo Wu,Chunlian Qin,Qunchen Yuan,Yingying Xue,Yizhou Xiong,Liujing Zhuang,Ning Hu,Ping Wang
Among basic taste sensations, bitter taste is vital to the survival of mammals due to its indispensable role in toxin prediction or identification, so the identification of bitter compounds is of great value in the pharmaceutical and food industry. Recently, bitter taste receptor (T2Rs)-based biosensors have been developed for specific bitter detection. However, the taste biosensors based on taste cells/tissues suffer from simple function, low sensitivity, low content, and limited parameters. Here, to establish a high-content, highly sensitive, and multifunctional taste biosensor, we developed a multifunctional hybrid integrated cardiomyocyte biosensor (HICB) for bitter detection. Due to the expression of bitter taste receptors in cardiomyocytes, the HICB can recognize the specific bitter agonists by synchronously recording the extracellular field potential (EFP) and mechanical beating (MB) signals from the cultured cardiomyocytes in vitro. Multiple feature parameters were defined and extracted from the electromechanical signals of cardiomyocytes to analyze the specific responses to four typical bitter compounds. The radar map, heat map, and principal component analysis (PCA) were used to visualize and classify the specific responses. Moreover, bitter-induced cardiotoxicity also was chronically evaluated, and these bitter compounds presented an inhibition effect on the electrophysiological and contractile activities of cardiomyocytes. This high-content HICB offers an alternative platform for both bitter detection and cardiotoxicity assessment, showing promising applications in the fields of taste detection and toxicity screening.
研究领域:
生物技术
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药理学
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毒理学
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细胞生物学
·
感觉生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD AND DRUG ANALYSIS
·
2016
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IF 1.98
Ming-Chao Cui,Shao-Jun Chen,Han-Hua Wang,Zhao-Hui Li,Hong-Jiang Chen,Yong Chen,Hai-Bin Zhou,Xiang Li,Jian-Wei Chen
Thunberg fritillary bulb (the dry bulbs of Fritillaria thunbergii Miq.), a traditional Chinese Medicine, is widely applied as an expectorant and antitussive. In this investigation, the primary metabolites of bulbs, flowers, leaves, and stems of F. thunbergii were analyzed by gas chromatography–mass spectrometry. Principal component analysis, partial least squares-discriminate analysis, orthogonal projection to latent structures-discriminate analysis, and heat map analysis showed that there were dissimilar metabolites, and a negative correlation between amino acids and saccharides in different analytes. Furthermore, carbodiimide, tryptophan, glucose-6-phosphate, xylose, 2-piperidinecarboxylic acid, monoamidomalonic acid, phenylalanine, and histidine were found to play an important role in the plant metabolism net of F. thunbergii .
研究领域:
药用植物学
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分析化学
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植物代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Horticulturae
·
2022
·Vol. 8
·期 11
·1029
·
IF 2.923
Qing-Fang Xu,Liang Fan,Kai-Xiang Lu,Dong-Mei Zhao,Ming-Xia Zhang,Jian Cai
Both free and glycosidically bound forms of volatile compounds in Vidal grapes from the Shangri-La high-altitude region during the on-vine non-destructive dehydration process were investigated by headspace solid-phase micro-extraction (HS-SPME) combined with gas chromatography–mass spectrometry (GC-MS), following which the data were processed by multivariate statistical analysis. Fatty-acid-derived volatiles (FADs), amino-acid-derived volatiles (AADs), and isoprene-derived volatiles (IPDs), which occurred mainly in bound forms, were the three major volatiles in dehydrated Vidal grapes. Water-loss concentration, biosynthesis, and biodegradation all occurred during dehydration, eventually modifying some volatiles significantly, especially some powerful odorants such as hexanal,trans-2-hexenal, 2-phenethyl acetate, β-myrcene, linalool, geraniol,cis-rose oxide, and β-damascenone. 1-Octen-3-ol was relatively stable during the non-destructive on-vine dehydration process and its content in grape juice was mainly determined by the concentration effect. 2,4-Di-tert-butylphenol, 2-phenethyl acetate, 2-methyl-1-propanol, and hexanol were screened as some of the most important metabolic markers to discriminate grapes at different dehydration degrees. Our study also highlights the fundamental importance of the expression of volatile content in the metabolomic study of grape berries.Keywords:late harvest;over-ripening;dessert wine;metabolomics;clustered heatmap;OPLS-DA
研究领域:
农学
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食品科学
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分析化学
·
植物生理学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）