Microsystems & Nanoengineering
·
2024
·Vol. 10
·期 1
·1-9
·
IF 7.3
Li Fuwang,Wei Jian,Qin Xiaomei,Chen Xue,Chen Dawei,Zhang Wentao,Han Jiaguang,Yuan Libo,Deng Hongchang
Field-controlled micromanipulation represents a pivotal technique for handling microparticles, yet conventional methods often risk physical damage to targets. Here, we discovered a completely new mechanism for true noncontact manipulation through photothermal effects, called thermal-optical tweezers. We employ a laser self-assembly photothermal waveguide (PTW) for dynamic microparticle manipulation. This waveguide demonstrates superior photothermal conversion and precision control, generating a nonisothermal temperature field. The interaction of thermal convection and thermophoresis within this field creates a microfluidic potential well, enabling noncontact and nondestructive particle manipulation. By varying the path of PTWs in lithography and manipulating laser loading modes, diverse manipulation strategies, such as Z-shaped migration, periodic oscillation, and directional transport, are achievable. Our innovative noninvasive micromanipulation technology minimizes not only physical damage to target objects but also enables precise and diverse manipulation of micro entities, opening up new avenues for the photothermal control of cells and biomolecules.
研究领域:
生物物理学
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光学工程
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细胞生物学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Chen Wang,Guangming Ma,Shanqi Zhang,Kunhong Zhao,Xiangyang Li
The Tobamovirus helicase plays an important role in virus proliferation and host interaction. They can also be targets for antiviral drugs. Tobacco mosaic virus (TMV) is well controlled by ningnanmycin (NNM), but whether it acts on other virus helicases of Tobamovirus virus is not clear. In this study, we expressed and purified several Tobamovirus virus helicase proteins and analyzed the three–dimensional structures of several Tobamovirus virus helicases. In addition, the binding of Tobamovirus helicase to NNM was also studied. The docking study reveals the interaction between NNM and Tobamovirus virus helicase. Microscale Thermophoresis (MST) experiments have shown that NNM binds to Tobamovirus helicase with a dissociation constant of 4.64–12.63 μM. Therefore, these data are of great significance for the design and synthesis of new effective anti–plant virus drugs.
研究领域:
植物学
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病毒学
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药物化学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Zheng-Hong Liang,Rui Feng,Jia-Min Wu,Dong Li,Fang Wang,Xiu-Li Wang,Yu-Zhong Wang,Fei Song
Since the global water shortage, fog collection based on functional materials and surfaces has become one of the most potential ways to relieve the crisis. However, further improving the water collection efficiency through elaborate designs of material structures still encounters a bottleneck. Here, we present a temperature gradient-induced strategy for enhancing water collection from fog. Such a temperature modulation generates increases in the fog collection efficiency and surface-refreshing frequency by 25% and 217%, respectively. Scattering measurements show that the movement velocity of fog particles near cooling surfaces is increased by approximately 150%. Based on the theory of heat transfer and thermophoresis, the temperature field acting on fog particles is analyzed, deducing that the enhanced fog-collection performance results from thermophoretic forces generated by temperature gradients. This work offers a new approach for designing next-generation fog-collection devices and manipulating particle motions.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
Materials Today Sustainability
·
2023
·
IF 7.8
Ye Fu,Liubin Li,Shulun Ai,Zhiguang Guo,Weimin Liu
The acquisition of global freshwater resources has always been the direction of people's efforts. At present, metal materials based on special wettability and special structure have become an effective way to collect fog. However, it is still a bottleneck to further improve the water collection efficiency by carefully designing the wettability and special structure of materials. Therefore, we propose to use temperature gradient to enhance fog collection in metal surface. The mechanism of temperature gradient enhancement improves fog collection efficiency by 54 % and surface refresh rate by about 541 %. Further scattering measurements show that the capture time of fog particles at low cooling surface is shortened by about 40 %. Based on the theory of heat transfer and thermophoresis , the temperature gradient around the metal and the behavior of fog particles are analyzed, and it is concluded that the enhancement of fog collection performance on the metal surface can be caused by the temperature gradient caused by thermophoresis force. This work provides ideas for designing better fog collection equipment and materials.
研究领域:
材料科学
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环境科学
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热力学
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DOI
高影响因子
Nanotechnology and Precision Engineering
·
2021
·Vol. 4
·期 1
·
IF 0
Li Yanna,Fu Muqing,Pang Wei,Chang Ye,Duan Xuexin
Ambient suspended particulate matter (PM) (primarily with particle diameter 2.5 µm or less, i.e., PM2.5) can adversely affect ecosystems and human health. Currently, optical particle sensors based on light scattering dominate the portable PM sensing market. However, the light scattering method has poor adaptability to different-sized PM and adverse environmental conditions. Here, we design and develop a portable PM sensing microsystem that consists of a micromachined virtual impactor (VI) for particle separation, a thermophoretic deposition chip for particle collection, and an extended-gate field-effect transistor (FET) for particle analysis. This system can realize on-site separation, collection, and analysis of aerosol particles without being influenced by environmental factors. In this study, the design of the VI is thoroughly analyzed by numerical simulation, and mixtures of different-sized silicon dioxide (SiO 2 ) particles are used in an experimental verification of the performance of the VI and FET. Considering the low cost and compact design of the whole system, the proposed PM analysis microsystem has potential for PM detection under a wide range of conditions, such as heavily polluted industrial environments and for point-of-need outdoor and indoor air quality monitoring. A proposed microsystem system potentially provides accurate and calibration-free measurements based on a combination of front-end particulate matter (PM) separation and back-end PM detection. Silicon dioxide particles are used to verify the performance of the system’s virtual impactor, and an accurate cutoff diameter of 2 μm in low air flow rates can be obtained. Experimental results suggest that the proposed microsystem has potential for accurate real-time analysis of PM pollution under complex conditions.
研究领域:
环境科学
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微纳米技术
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传感器技术
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产品关联
DOI
International Journal of Peptide Research and Therapeutics
·
2019
·Vol. 26
·期 1
·121-128
·
IF 1.219
Wang Mengwei,Lv Mingxiu,Lu Kui,Liu Guangbin,Mai Wenpeng,Yu Bo,Lou Yudan
Six breast cancer susceptibility gene 1 (BRCA1) peptide fragments were designed by removing N-terminal amino acid residues of BRCA1(846–871). Peptide fragments BRCA1(846–871), BRCA1(852–871), BRCA1(854–871), BRCA1(856–871), BRCA1(857–871) and BRCA1(861–871) were obtained by Fmoc solid-phase synthesis, purified by reversed-phase high-performance liquid chromatography and characterized by electrospray ionization mass spectrometry. The interaction between BRCA1 fragment peptides and RAD51(181–200) target peptides were investigated using circular dichroism (CD) spectroscopy, fluorescence spectroscopy, and microscale thermophoresis (MST). The results of CD spectroscopy showed that the secondary structures of RAD51(181–200) were altered after the addition of BRCA1 peptide fragments. The fluorescence results proved that all six BRCA1 peptide fragments BRCA1(846–871), BRCA1(852–871), BRCA1(854–871), BRCA1(856–871), BRCA1(857–871) and BRCA1(861–871) could bind to RAD51(181–200) independently, their quenching processes were static, and the binding constant of peptide fragments with RAD51(181–200) were 4.01 × 10 5 L mol −1 , 7.22 × 10 5 L mol −1 , 7.84 × 10 5 L mol −1 , 1.1 × 10 4 L mol −1 , 6.19 × 10 3 L mol −1 , 6.18 × 10 3 L mol −1 . The results of MST displayed that BRCA1(854–871) could combine with RAD51(181–200), and the dissociation constant K d was 126.44 µM. Compared with other five peptide fragments, the interaction between BRCA1(854–871) and RAD51(181–200) was much stronger.
研究领域:
生物化学
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分子生物学
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癌症研究
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DOI
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 8
·1900
·
IF 3.098
Qifan Sun,Xin Gao,Hongna Bi,Yingbo Xie,Lin Tang
In this paper, the interaction between bovine lactoferrin (bLf) and tetracycline hydrochloride (TCH) was researched by microscale thermophoresis (MST), multi-spectroscopic methods, and molecular docking techniques. Normal fluorescence results showed that TCH effectively quenched the intrinsic fluorescence of bLf via static quenching. Moreover, MST confirmed that the combination force between bLf and TCH was very strong. Thermodynamic parameters and molecular docking further revealed that electrostatic forces, van der Waals, and hydrogen bonding forces played vital roles in the interaction between bLf and TCH. The binding distance and energy transfer efficiency between TCH and bLf were 2.81 nm and 0.053, respectively. Moreover, the results of circular dichroism spectra (CD), ultraviolet visible (UV-vis) absorption spectra, fluorescence Excitation-Emission Matrix (EEM) spectra, and molecular docking verified bLf indeed combined with TCH, and caused the changes of conformation of bLf. The influence of TCH on the functional changes of the protein was studied through the analysis of the change of the bLf surface hydrophobicity and research of the binding forces between bLf and iron ion. These results indicated that change in the structure and function of bLf were due to the interaction between bLf and TCH.Keywords:bovine lactoferrin;tetracycline hydrochloride;mutil-spectral technique;microscale thermophoresis;molecular docking
研究领域:
生物化学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2021
·Vol. 118
·期 42
·e2104481118
·
IF 11.205
Jintao Tong,Dalei Wang,Ye Liu,Xin Lou,Jiwei Jiang,Bin Dong,Renfeng Dong,Mingcheng Yang
In nature, microorganisms could sense the intensity of the incident visible light and exhibit bidirectional (positive or negative) phototaxis. However, it is still challenging to achieve the similar biomimetic phototaxis for the artificial micro/nanomotor (MNM) counterparts with the size from a few nanometers to a few micrometers. In this work, we report a fuel-free carbon nitride (C3N4)/polypyrrole nanoparticle (PPyNP)-based smart MNM operating in water, whose behavior resembles that of the phototactic microorganism. The MNM moves toward the visible light source under low illumination and away from it under high irradiation, which relies on the competitive interplay between the light-induced self-diffusiophoresis and self-thermophoresis mechanisms concurrently integrated into the MNM. Interestingly, the competition between these two mechanisms leads to a collective bidirectional phototaxis of an ensemble of MNMs under uniform illuminations and a spinning schooling behavior under a nonuniform light, both of which can be finely controllable by visible light energy. Our results provide important insights into the design of the artificial counterpart of the phototactic microorganism with sophisticated motion behaviors for diverse applications.
研究领域:
纳米技术
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生物物理学
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Rong Xu,Yue Gu,Lin Zheng,Mingtao Huang,Mouming Zhao
Previously, we presented a green and efficient separation method for soluble undenatured type II collagen (SC II) based on the complex coacervation of SC II and anionic polysaccharides , and demonstrated the significant impact of molecular weight (M w ). In this study, we combined differential scanning calorimetry (DSC), microscale thermophoresis (MST) and molecular dynamics simulation (MDS) to further explore the affinity and interaction types of SC II with different anionic polysaccharides . Results showed that the denaturation temperature of SC II increased (about 3 °C) after complex coacervating with chondroitin sulfate (CS, a small anionic polysaccharide), which might be related to their strong affinity (dissociation constant of K d and binding energy were about 3.04 × 10 −6 mg/mL and −4054.97 kJ/mol, respectively), and the molecular weight of anionic polysaccharides had a greater impact than chain conformation. The non-covalent interactions between SC II and anionic polysaccharides were mainly hydrogen bonds, followed by hydrophobic interactions and ionic bonds. The hydroxyl and sulfate groups in polysaccharides could form extensive hydrogen bonds and small amount of ionic bonds with a large number of amino acids in SC II, and the hydrophobic groups such as methyl also formed hydrophobic interactions with hydrophobic parts in SC II (such as Pro82, Pro85, Val88). Importantly, the smaller the M w of anionic polysaccharides, the faster they formed stable interactions with SC II. Therefore, anionic polysaccharides with smaller M w had higher affinity and faster non-covalent interactions formation speed with SC II, resulting in more compact structure and higher thermal stability of the complexes.
研究领域:
生物化学
·
分子生物学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Shuang Mu,Zhaowei Tian,Wei Ren,Chenghui Liu
Surface-enhanced Raman scattering (SERS) has emerged as a powerful tool for contamination detection. Fabricating efficient nanostructures with hotspots for signal enhancement and concentrating diluted target analyte molecules to the hotspots are critical for ultrasensitive SERS detection, which generally requires advanced instruments and intricate manipulations. Herein, we report a simple, low-cost, and high-efficiency paper device that can simultaneously concentrate the analytes and generate SERS hotspots rapidly with the assistance of laser-induced thermophoresis. After dropping the target- and plasmonic nanoparticle-containing solution on a paper substrate, the evaporative gradient created by the laser-induced thermophoresis can promote the delivery of the analytes and plasmonic nanoparticles simultaneously to the tiny area of the laser spot, forming compact SERS hotspots to significantly amplify the analyte’s Raman scattering signals. This convenient thermophoretic strategy can be accomplished rapidly within ∼4 min and exhibits more than 104-times higher sensitivity than that without the assistance of laser-based thermophoresis. This elegant paper device is successfully applied to the detection of contaminants such as pesticides and nanoplastics in fruit and water samples, holding the potential to provide a simple, fast, and cost-effective approach for on-site detection of environmental contaminants.
研究领域:
分析化学
·
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2015
·
IF 3.545
Yexuan Mao,Lanlan Yu,Ran Yang,Chuanguo Ma,Ling-bo Qu,Peter de B. Harrington
The formation of highly ordered fibrils for the human islet amyloid polypeptide (hIAPP) is considered as one of the precipitating factors of type 2 diabetes mellitus. In this study, an emerging new approach microscale thermophoresis and conventional ThT fluorescence assay were utilized to investigate the aggregation behavior of hIAPP 11–20 , giving a new insight of the solvent effect on the aggregation of hIAPP 11–20 . hIAPP 11–20 displayed different aggregation behaviors in various buffers, revealing that hIAPP 11–20 not only self-aggregates but also binds to solvent components. hIAPP 11–20 had a higher binding affinity for Tris than other selected buffers because multiple hydrogen bonds form, resulting in weaker self-aggregation of hIAPP 11–20 at the early stage of aggregation and prolonging the fibril formation process. hIAPP 11–20 displayed similar self-aggregation in both HEPES and pure water. Negatively charged phosphate ions in the PBS solution ‘neutralize’ the charges carried by hIAPP 11–20 itself to some extent, causing rapid aggregation of hIAPP 11–20 , and leading to a shorter fibrillation process of hIAPP 11–20 . These results revealed that solvents contribute to the aggregation of hIAPP 11–20 and demonstrated the affect of solvents on the activity of biomolecules. Additionally, as a new technique, microscale thermophoresis offers a powerful and promising approach to study the early stages of aggregation of peptides or proteins.
研究领域:
生物化学
·
分子生物学
·
糖尿病研究
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2017
·
IF 2.896
Yexuan Mao,Lanlan Yu,Ran Yang,Chuanguo Ma,Lingbo Qu,Peter de B. Harrington
The structural transition and misfolding of human islet amyloid polypeptide may cause a common metabolic disease Type 2 diabetes mellitus . Seventeen peptides have been synthesized, possessing different lengths, compositions , and peptide conformation. In this study, the mechanism of these peptides on inhibiting the formation of hIAPP 11–20 amyloid fibrils was investigated using a conventional ThT fluorescence assay and microscale thermophoresis. The results showed that short peptides AT, SA, RF, KS, KT and KN, and cyclic peptides cyclic-KS, cyclic-KT and cyclic-KN displayed considerable inhibitory effect on hIAPP 11–20 fibril formation and a strong affinity for hIAPP 11–20 . The detailed investigation indicated that the phenylalanine residue and some special peptide composition significantly inhibit amyloid formation. The peptide conformation of the designed peptide inhibitors may also play an important role. Microscale thermophoresis quantified the binding affinities between hIAPP 11–20 and the peptides; and revealed that high affinity binding more likely leads to inhibiting fibril formation of hIAPP 11–20 and vice versa, which is in accordance with the results from the ThT assays. These findings suggest a feasible model of peptide inhibitor design for inhibiting amyloid formation. In addition, microscale thermophoresis was proven as a promising rapid method for preliminarily screening inhibitors of hIAPP 11–20 .
研究领域:
生物化学
·
分子生物学
·
代谢疾病研究
✓ 已验证
DOI
高影响因子
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