mBio
·
2024
·Vol. 15
·期 3
·
IF 5.1
Yilin Xiao,Luyao Gao,Zhiyong Li
Global warming is a key issue that causes coral bleaching mainly because of the thermosensitivity of zooxanthellae. Compared with the well-studied zooxanthellae Symbiodiniaceae in coral holobionts, we rarely know about other coral symbiotic algae, let alone their thermal tolerance. In this study, a zoochlorellae, Symbiochlorum hainanensis, isolated from the coral Porites lutea, was proven to have a threshold temperature of 38°C. Meanwhile, unique high-temperature tolerance mechanisms were suggested by integrated transcriptomics and real-time quantitative PCR, physiological and biochemical analyses, and electron microscopy observation. Under heat stress, S. hainanensis shared some similar response strategies with zooxanthellae Effrenium sp., such as increased ascorbate peroxidase, glutathione peroxidase, superoxide dismutase activities and chlorophyll a, thiamine, and thiamine phosphate contents. In particular, more chloroplast internal layered structure, increased CAT activity, enhanced selenate reduction, and thylakoid assembly pathways were highlighted for S. hainanensis’s high-temperature tolerance. Notably, it is the first time to reveal a whole selenate reduction pathway from SeO42− to Se2− and its contribution to the high-temperature tolerance of S. hainanensis. These unique mechanisms, including antioxidation and maintaining photosynthesis homeostasis, efficiently ensure the high-temperature tolerance of S. hainanensis than Effrenium sp. Compared with the thermosensitivity of coral symbiotic zooxanthellae Symbiodiniaceae, this study provides novel insights into the high-temperature tolerance mechanisms of coral symbiotic zoochlorellae S. hainanensis, which will contribute to corals’ survival in the warming oceans caused by global climate change.
研究领域:
海洋生物学
·
环境科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2024
·
IF 5.5
Yang Yang,Xuanhe You,Tao Deng,Mingyang Li,Yuan Liu,Mingjie Xu,Yong Nie,Shi-Mei Xu,Bin Shen
Hydrogels are considered as a potential cartilage replacement material based on their structure being similar to natural cartilage, which are of great significance in repairing cartilage defects. However, it is difficult for the existing hydrogels to combine the high load bearing and low friction properties (37 °C) of cartilage through sample methods. Herein, we report a facile and new fabrication strategy to construct the PNIPAm/EYL hydrogel by using the macrophase separation of supersaturated N-isopropylacrylamide (NIPAm) monomer solution to promote the formation of liposomes from egg yolk lecithin (EYL) and asymmetric template method. The PNIPAm/EYL hydrogels possess a relatively high compressive strength (more than 12 MPa), fracture energy (9820 J/m2), good fatigue resistance, lubricating properties, and excellent biocompatibility. Compared with the PNIPAm hydrogel, the friction coefficient (COF 0.046) of PNIPAm/EYL hydrogel is reduced by 50%. More importantly, the COF (0.056) of PNIPAm/EYL hydrogel above lower critical solution temperature (LCST) does not increase significantly, exhibiting heat-tolerant lubricity. The finite element analysis further proves that PNIPAm/EYL hydrogel can effectively disperse the applied stress and dissipate energy under load conditions. This work not only provides new insights for the design of high-strength lubricating hydrogels but also lays a foundation for the treatment of cartilage injury as a substitute material.
研究领域:
生物材料学
·
组织工程学
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产品关联
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 21
·8469-8479
·
IF 4.813
Meng Kang-Li,Lv Xin-Chen,Che Han-Yu,Li Yue,Chen Xian-Lin,Hu Meng-Xin,Yan Ming
Abstract Biofilms are heterogeneous structures composed of microorganisms and the surrounding extracellular polymeric substances (EPS) that protect the microbial cells from harsh environments. Saccharomyces boulardii is the first yeast classified as a probiotic strain with unique properties. However, tolerance of S. boulardii biofilms to harsh environments especially during production and in the gastrointestine remains unknown. In this study, S. boulardii cells were encapsulated in alginate microcapsules and subsequently cultured to form biofilms, and their survival and tolerance were evaluated. Microencapsulation provided S. boulardii a confined space that enhanced biofilm formation. The thick alginate shell and the mature biofilm improved the ability of S. boulardii to survive under harsh conditions. The exogenous encapsulation and the endogenous biofilm structure together enhanced the gastrointestinal tolerance and thermotolerance of S. boulardii. Besides, as the alginate shell became thinner with an increase in the subsequent culture duration, the EPS of S. boulardii biofilms exerted an important protective effect in resisting high temperatures. The encapsulated biofilm of S. boulardii after 24-h culture exhibited 60 × higher thermotolerance at 60 °C (10 min), while those after 6-h and 24-h culture showed 1000 × to 550,000 × higher thermotolerance at 120 °C (1 min) compared with the planktonic cells without encapsulation. The present study’s findings suggest that a combination of encapsulation and biofilm mode efficiently enhanced gastrointestinal tolerance and thermotolerance of S. boulardii. Key points
• Encapsulated S. boulardii in biofilm mode showed enhanced tolerance.
• Exogenous shell and endogenous biofilm provided dual protection to S. boulardii.
Graphical abstract
研究领域:
微生物学
·
食品科学
·
生物医药
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Yuan Liang,Mengjun Hu,Rui Dong,Shoukui He,Yan Cui,Jinzeng Yang,Xiangyu Deng,Xianming Shi
Sodium hypochlorite (NaClO) is a widely used disinfectant in food processing environments. However, its improper use can lead Salmonella to develop tolerance to other stresses, which threatens food safety and human health. In this study, the survival ability assays showed that adaptation of S. Enteritidis to NaClO resulted in the development of heat tolerance at 55 °C and 63 °C. NaClO-adapted S. Enteritidis also showed a survival benefit in milk at 55 °C and 63 °C. NaClO-adapted S. Enteritidis exhibited a decrease in accumulation of reactive oxygen species (ROS) compared to non-adapted S. Enteritidis after exposure to 63 °C. Moreover, compared to non-adapted S. Enteritidis, the membrane fatty acids C16:0 and cyclo-heptadecanoic acid Cyc17 were significantly increased in NaClO-adapted S. Enteritidis, whereas UFA to SFA ratio was observed to decrease from 0.52 ± 0.04 to 0.41 ± 0.04. These changes resulted in a decrease in membrane fluidity of NaClO-adapted S. Enteritidis. Furthermore, RT-qPCR analysis revealed a considerable increase in the expression levels of heat shock-related genes in NaClO-adapted S . Enteritidis with or without heat treatment, in comparison to non-adapted S . Enteritidis. It was noteworthy that upregulation was 7.30-fold for dnaK , 11.62-fold for ibpA , and 3.92-fold for rpoH under heat treatment, suggesting that these genes played important roles in the development of heat tolerance. Taken together, adaptation of S . Enteritidis to NaClO resulted in the development of heat tolerance, which might increase the risk of cross-contamination and salmonellosis in the food industry.
研究领域:
食品安全
·
微生物学
·
应激反应
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Energy Materials
·
2022
·
IF 6.959
Peilong Zhou,Dongxu Yao,Hanqin Liang,Jinwei Yin,Yongfeng Xia,Yu-Ping Zeng
A robust flame-retardant separator was fabricated by a nonsolvent-induced phase separation method incorporating polyimide with silicon nitride (Si3N4) whiskers. Compared with powder-blended separators, whisker-blended separators not only suppressed the agglomeration of fillers but also improved the internal connectivity of the separator. These advantages were conducive to boosting Li ions and improving the cycling stability. The whisker-blended separator exhibited a high heat endurance in air (400 °C for 1 h). Cells assembled with this separator exhibited a high initial discharge capacity (152 mA h g–1), an enhanced capacity retention of 79.4% (120 mA h g–1), and a high Coulombic efficiency of 97.5% after 300 cycles.
研究领域:
材料科学
·
能源技术
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2015
·Vol. 6
·期 7
·5621-5630
·
IF 3.84
Zheng Su,Hua Wang,Fei Xu,Konghu Tian,Jingwei Wu,Xingyou Tian
We report a simple and environmentally friendly approach for preparing a homogeneous dispersion of poly(2-ethylhexyl acrylate)–silicon dioxide (P2EHA–SiO2) composites by self-assembly of negatively charged P2EHA and charged SiO2. The P2EHA latex particles were prepared by miniemulsion polymerization using a free-radical initiator. As auxiliary monomer, acrylic acid created the positive charges on the surface of the P2EHA latex. By mixing P2EHA latex particles with positively charged SiO2 (pSiO2) particles, negatively charged P2EHA latex particles easily assembled with pSiO2 particles through electrostatic interactions. The presence of P2EHA latex particles assisted the homogeneous dispersion of pSiO2 particles in the polymer matrix. After film formation process, the film exhibited excellent thermal-tolerance and adhesive properties. A three-component system of P2EHA latex particles, pSiO2, and negatively charged SiO2 (nSiO2) was also achieved successfully due to the mutual assistance effect. A better thermal-tolerance and adhesive properties were obtained. Through the establishment of the film formation mechanism, we found that the architecture of the composite film has a significant influence on thermal-tolerance and adhesive properties. This composite material has great application potential as a PSA (pressure sensitive adhesive). Our research demonstrates an excellent composite system and provides a convenient approach to realize a wide range of multifunctional fillers, compatible with processing on flexible polymer substrates.
研究领域:
材料科学
·
聚合物化学
✓ 已验证
DOI
高影响因子
SOLAR ENERGY MATERIALS AND SOLAR CELLS
·
2019
·
IF 6.019
Xuwen Yang,Shan Cong,Juan Li,Jian Chen,Feng Jin,Zhigang Zhao
A new type of gel polymer electrolyte composed of free-standing aramid nanofibers was used to fabricate all-solid-state near-infrared (NIR) electrochromic devices for NIR sheltering applications. This new type of gel polymer electrolyte showed excellent mechanical and heat endurance compared with currently available gel polymer electrolytes. The good film-forming ability of the aramid nanofibers makes assembling the electrochromic devices more convenient. The as-fabricated device showed an optical modulation of about 40% at 980 nm and achieved a coloration efficiency of 79.5 cm 2 C −1 , values that are larger than those previously reported for other electrochromic devices with similar structures. It is expected that aramid nanofibers-LiClO 4 -based electrolyte films with high mechanical and heat endurance will have potential applications in spacecraft thermal control, optical communication , and biomedical analysis.
研究领域:
材料科学
·
电化学
·
光学工程
✓ 已验证
产品关联
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2023
·
IF 6.6
Chao Ma,Xiaoqian Zhang,Kaiqiang Wang,Jingya Jiang,Jianhua Zeng,Lanwei Zhang,Pimin Gong
Employing 0.64 mg/mL of rifampin (RFP) for dormancy induction of Lactobacillus bulgaricus sp 1.1, a 2.5-fold survival rate was achieved after spray drying, while the dried bacteria displayed delayed acidifications . Under the same condition for dormancy treatment, division analysis indicated that high proportions of cells would enter into a dormant state with around 90% of non-proliferating bacteria in the original population. Cells shrank with median diameters decreasing from 3.23 to 2.98 μm. The Raman spectrum revealed a lower ATP level, varied protein expression profiles and conformations and saccharides accumulation in bacteria. Protein aggregates formed in dormancy-induced cells may contribute to increased heat resistance which showed a 2 °C rise in the denaturing temperature in the 0.64 mg/mL group. Therefore, dormancy treatments may be developed as a protective strategy for spray-dried L . bulgaricus . Industrial relevance Enhancing the drying tolerance of bacteria is one of the crucial strategies to adapt the industrial production of spray-dried LAB . Our study proved that the non-sporeforming dormancy pre-treatment can promote the spray-dried viability of L . bulgaricus by triggering suppressed metabolism activity and protein aggregation. The non-sporeforming dormant state has potential to be exploited in industrial production of spray-dried LAB with a high viable cell density to reduce the cost of probiotics and related products.
研究领域:
微生物学
·
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Science
·
2024
·
IF 5.8
Jinchao Zhao,Lei Lei,Wenbin Dai,Angfeng Jiang,Qiao Jin,Zhe Tang
Radiofrequency ablation (RFA) is a commonly used minimally invasive treatment for hepatocellular carcinoma (HCC). However, incomplete radiofrequency ablation (iRFA) promotes tumor progression and metastasis. There is an urgent need to develop innovative strategies to enhance the efficacy of iRFA. The upregulation of heat shock proteins (HSPs) and activation of protective autophagy in tumor cells upon exposure to sublethal heat enhance the thermotolerance, thereby promoting tumor cell survival. Here, 3-methyladenine (3-MA) and lonidamine (LND) co-encapsulated liposomes (Lip@LND/3-MA) are designed to enhance the efficacy of iRFA by simultaneous inhibition of glycolysis and autophagy. On one hand, LND inhibits hexokinase, a key enzyme in glycolysis, and thus reduces ATP production and consequently suppresses the expression of HSPs. On the other hand, 3-MA, as an autophagy inhibitor, can inhibit protective autophagy after iRFA. Lip@LND/3-MA is confirmed to suppress the expression of HSPs and reduce the autophagy level during RFA. Therefore, the thermotolerance of tumor cells is significantly weakened, leading to remarkably enhanced therapeutic efficacy of iRFA. It is believed that simultaneous inhibition of HSPs and autophagy is a promising therapeutic strategy in clinical practice of RFA.
研究领域:
肿瘤学
·
生物医学工程
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Ziqi Liu,Yuechong Liu,Jian He,Xiaoyan Pei,Yixin Lin,Marciane Magnani,Donghong Liu,Tian Ding,Jinsong Feng
The bacterial contamination and failure of pasteurization are leading threats to the safety of dairy productions. Milk contains a large quantity of fat which supports bacterial growth but its influence on pasteurization efficacy and biofilm formation remains poorly understood. In this study, we first demonstrate that the inactivation of Salmonella during pasteurization is significantly less effective in whole milk compared to skim milk, with the D-value increasing from 0.60 min in skim milk to 0.67 min in whole milk ( P = 0.0047). In whole milk, Salmonella biofilms form greater biomass and display a distinct architecture from biofilms in skim milk. Specifically, Salmonella in whole milk was embedded within the extracellular polymeric substances (EPS), while cells in skim milk biofilms were sparsely distributed. Raman spectroscopy showed that biofilms formed in whole milk possess a distinctly higher lipid content. Therefore, we hypothesized that the thermal tolerance of Salmonella in whole milk was directly linked to its physical interaction with milkfat. Confocal laser scanning microscopy showed an increase from 26.1 % to 86.1 % of the fraction of Salmonella co-localizing with milkfat after 12 h of incubation in whole milk. Lipase treatment (200 units/mL) on whole milk reduced biofilm formation and improved the efficacy of thermal inactivation. Our findings demonstrate the milkfat acts as a physical scaffold, facilitating bacterial aggregation and the formation of a protective, lipid-rich biofilm matrix enhancing Salmonella survival during pasteurization. These insights should inform pasteurization strategies of high-fat dairy products to ensure food safety.
研究领域:
食品微生物学
·
食品安全
·
应用微生物学
·
乳品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2017
·Vol. 5
·期 12
·3088-3096
·
IF 5.256
Xinmin Cui,Ruimin Ding,Mengchao Wang,Conghui Wang,Jing Zhang,Jing Wang,Wensheng Dong,Yao Xu
An ultralow-index top layer is a prerequisite to preparing high-performance broadband AR coatings. When coupled with hydrophobicity and the abrasion-resistance required in practical applications, the challenge becomes even greater. In this work, a MgF2 AR coating was studied because of the low refractive index of MgF2 and its easily realized strong adhesion through low-temperature heat treatment. In order to obtain an ultralow refractive index and endow the coating with hydrophobicity, we designed several experimental routes and finally adopted MTES/TEOS co-precursors to direct MgF2 particles to form a honeycomb-like network structure without a template. A final refractive index of 1.15 and good hydrophobicity, with a water contact angle of 122°, were obtained using the MgF2–SiO2(CH3) coating. These superior properties were attributed to the incorporation of methyl groups, which not only endowed the coating with hydrophobicity, but also changed the original linear assembly of MgF2 particles to a circular assembly. Using this hydrophobic ultralow-index coating as a top layer, a high-performance double-layer AR coating was fabricated with a high average transmittance of 99.43% in the wavelength range of 400–1000 nm, good abrasion-resistance, and damp heat resistance after a low-temperature heat treatment of 250 °C. This MgF2 double-layer AR coating may be used in display devices or lenses.
研究领域:
材料科学
·
光学
·
表面工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）