Heliyon
·
2024
·
IF 3.4
Yuanyuan Meng,Shuangxi Li,Zhi Zhang
Corrosion inhibitors play a vital role in impeding the corrosion process of steel bars within concrete structures exposed to corrosive environments. Nevertheless, conventional corrosion inhibitors pose environmental risks. In contrast, contemporary studies have explored corrosion inhibitors that are eco-friendly. However, these inhibitors are burdened by high costs and complex production processes, impeding the widespread application in concrete structures. Consequently, this study presents an innovative solution by incorporating uniconazole, an agricultural fungicide, as a corrosion inhibitor for steel bars in concrete structures. The steel bars were exposed to corrosion within a simulated concrete pore solution containing 0.6 mol/L NaCl, both with and without the presence of uniconazole. The morphology and hydrophilicity of the steel bar surface were investigated via optical microscope and contact angle experiments. Electrochemical tests (open circuit potential, potentiodynamic polarization, electrochemical impedance spectroscopy, and Mott-Schottky analysis) and X-ray photoelectron spectroscopy were employed to investigate the corrosion inhibition performance and mechanism of uniconazole. The results demonstrate that uniconazole elevates the hydrophobicity and contributes to the corrosion inhibition of steel bars. Electrochemical test results indicate that as the concentration of uniconazole increases from 1 × 10 −4 mol/L to 1 × 10 −3 mol/L, the inhibition efficiency likewise demonstrates a corresponding increase, escalating from around 50 %–90 %. Uniconazole molecules function as mixed-type inhibitors, exhibiting characteristics of both anode-type and cathode-type inhibitors. The adsorption of uniconazole enhances the stability and thickness of the passive-adsorbed layer on the steel surface, effectively impeding the charge transfer process and obstructing the interaction of corrosive substances with the base metal. In summary, the application of uniconazole exhibits the highlights of efficient, cost-effective, environmentally friendly, and the potential for scalable production. This positions uniconazole as a promising candidate for use as a corrosion inhibitor in the domain of concrete structures.
研究领域:
土木工程
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材料科学
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环境科学
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DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2018
·
IF 3.677
Li Zhan,Gao Yue,Zhang Yuchan,Lin Cheng,Gong Dongting,Guan Yajing,Hu Jin
Seed germination is a complex process controlled by various mechanisms. To examine the potential contribution of reactive oxygen species (ROS) and gibberellin acid (GA) in regulating seed germination, diphenylene iodonium chloride (DPI) and uniconazole (Uni), as hydrogen peroxide (H2O2) and GA synthesis inhibitor, respectively, were exogenously applied on tobacco seeds using the seed priming method. Seed priming with DPI or Uni decreased germination percentage as compared with priming with H2O, especially the DPI + Uni combination. H2O2 and GA completely reversed the inhibition caused by DPI or Uni. The germination percentages with H2O2 + Uni and GA + DPI combinations kept the same level as with H2O. Meanwhile, GA or H2O2 increased GA content and deceased ABA content through corresponding gene expressions involving homeostasis and signal transduction. In addition, the activation of storage reserve mobilization and the enhancement of soluble sugar content and isocitrate lyase (ICL) activity were also induced by GA or H2O2. These results strongly suggested that H2O2 and GA were essential for tobacco seed germination and by downregulating the ABA/GA ratio and inducing reserve composition mobilization mutually promoted seed germination. Meanwhile, ICL activity was jointly enhanced by a lower ABA/GA ratio and a higher ROS concentration.
研究领域:
植物生理学
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分子生物学
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植物学
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PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 24
·
IF 5.1
Tailong Wei,Xiao Chen,Chong Cai,Yuanzhen Guo,Mengjun Zhou,Qiannan Gao,Qinghua He
Mimotope-based immunoassays offer an eco-friendly alternative to chemically synthesized antigens for the quantitative analysis of small molecules, but their use for practical on-site and high-throughput residue monitoring remains limited. Herein, we report the selection, production, and application of a phage display–derived mimotope targeting an anti-uniconazole monoclonal antibody (UCZ-mAb), with the aim of developing two complementary immunoassays that enable sensitive, eco-friendly detection of UCZ residues in agricultural samples. A 12-mer phage-displayed peptide library was screened to identify UCZ-specific mimotopes, and a selected sequence was genetically fused to SpyTag and expressed inEscherichia colito generate a SpyTagged mimotope. Leveraging the SpyCatcher/SpyTag self-assembly system, the SpyTagged mimotope was directionally conjugated onto SpyCatcher-functionalized time-resolved fluorescence beads (TRFBs) and subsequently used as a signal-labeled competitive antigen in a lateral flow immunoassay (LFIA) designed for rapid on-site screening. In parallel, a wash-free magnetic separation immunoassay (MSIA) suitable for green, high-throughput screening in routine laboratories was established using self-assembled mimotope-TRFB probes. The LFIA and MSIA exhibited half-maximal inhibitory concentrations (IC50) of 3.70–6.72 μg/kg and 16.4–18.3 μg/kg, respectively, in real samples. Spiked-sample recoveries ranged from 91.1 to 107.8% for LFIA and 92.6–115.7% for MSIA, demonstrating acceptable accuracy and precision. These results indicate that the SpyTagged mimotope–based LFIA and MSIA provide complementary, reliable, and sensitive platforms for on-site screening and high-throughput monitoring of UCZ residues in agricultural samples, while avoiding the drawbacks associated with traditional chemical antigen synthesis.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Rui Cheng,Yiqun Wan,Xuejin Mao,Hao Wan,Fan Liu,Lan Guo,Bingzhen Zhang
This work presents an efficient sorbent for plant growth regulators (PGRs) by regulating the defects of a metal–organic framework MIL-101(Cr). Using the regulated MIL-101(Cr), we developed a simple and effective method for the simultaneous determination of eleven PGRs in fresh fruit juice. The extraction conditions were optimized by an orthogonal array design. Under optimal conditions, the method showed a satisfactory limit of detection (0.1–1.2 ng/g), recovery rates (83.4–110.2 %), and precision (2.9–18.0 % for intra-day and 2.7–10.8 % for inter-day), as well as a greatly suppressed matrix effect. Notably, regulating the defects significantly enhanced the desorption of PGRs on MIL-101(Cr). The sorbent didn’t need to be destroyed to release the adsorbed PGRs and could be reused at least 6 times. Furthermore, the defects of MIL-101(Cr) and interactions between the sorbent and PGRs were studied by TGA, ATR-IR, XPS, NH 3 -TPD and UV–Vis DRS.
研究领域:
分析化学
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食品科学
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DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2017
·Vol. 40
·期 19
·3754-3762
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IF 2.557
Yuanjing Lin,Jialiang Guo,Hang Lin,Jincai Wang,Govert W. Somsen,Jacques Crommen,Zhengjin Jiang
To evaluate the effect of the preparation strategy on the enantioseparation performance of β-cyclodextrin-functionalized monoliths, a series of β-cyclodextrin-functionalized organic polymeric monolithic columns were prepared through two-step, single-step, and one-pot approaches, using the same cyclodextrin, linker–spacer, and crosslinker. Physicochemical characterization of the columns was carried out by determining the morphology, β-cyclodextrin density, permeability, and chromatographic efficiency. For each type of monolithic column, the enantioresolution of 22 chiral compounds, including mandelic acid derivatives, nonsteroidal anti-inflammatory drugs, N-derivatized amino acids, and herbicides, was comparatively studied under optimum chromatographic conditions. The β-cyclodextrin-functionalized monolithic columns prepared through the one-pot approach exhibited higher enantioresolution for most chiral compounds, and they have the advantage of good controllability and simple preparation. On the other hand, the enantioresolution obtained on columns prepared through the single-step approach was quite unsatisfactory, and therefore the effect of using different linking spacers and crosslinkers was studied. A significant improvement of enantioresolution for 2-chloro-mandelic acid was obtained by using N , N -methylenebisacrylamide instead of ethylene dimethacrylate as the crosslinker in the single-step preparation.
研究领域:
分析化学
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材料科学
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色谱技术
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Qingqing Zhang,Xuejin Mao,Changrong Yuan,Jiexue Zhao,Hui Hu,Aiping Yan,Yuanxing Wang,Weiming Xiao
A facile, efficient and reliable method was designed and established to analyze the plant growth regulators (PGRs) in citrus fruit, based on a simplified dispersive solid-phase extraction (d-SPE) using a shaped zirconium-based metal–organic framework (UiO-66-PDMS bead) as the sorbent. In this method, UiO-66-PDMS beads directly extracted the targets from the homogenized and could be easily separated with a tweezer. It avoided the centrifugation or filtration operation required in normal d-SPE, greatly simplifying the d-SPE process. Moreover, the matrix substances were efficiently removed by this d-SPE process. The method showed good linearities (R 2 ≥ 0.9995) and limits of detection (0.09–0.17 ng/g). The recoveries were in the range of 80.7–97.5 %. The intra-day and inter-day precisions were 1.5–6.3 % and 4.6–11.7 %, respectively. Additionally, the adsorption interactions between UiO-66-PDMS bead and PGRs were studied by ATR-FTIR and UV–vis DRS. Furthermore, the method was employed to screen the PGRs in ten different citrus fruits.
研究领域:
分析化学
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食品科学
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PubMed
高影响因子
TOXICOLOGY IN VITRO
·
2023
·
IF 3.2
Jing Nie,Jiabei Zhou,Yi Shen,Ruimiao Lin,Haihong Hu,Kui Zeng,Huichang Bi,Min Huang,Lushan Yu,Su Zeng,Jing Miao
The widespread use of triazole fungicides in agricultural production poses a potential risk to human health. This study investigates the interaction of five triazole fungicides, i.e., tebuconazole, triticonazole, hexaconazole, penconazole, and uniconazole with human renal transporters, including OAT1, OAT3, OCT2, OCTN1, OCTN2, MATE1, MATE2-K, MRP2, MDR1, and BCRP, using transgenic cell models. For uptake transporters, triticonazole was the substrate of OAT1 and OAT3 and the inhibitor of OCT2. Tebuconazole and penconazole inhibited OCTN2 (100 μM), while tebuconazole, triticonazole, hexaconazole, penconazole, and uniconazole inhibited MATE1 (100 μM). Tebuconazole and hexaconazole inhibited MATE2-K (100 μM). All five triazole fungicides were not substrates or strong inhibitors of MRP2, MDR1, and BCRP efflux transporters. Penconazole inhibited OCT2 with IC 50 = 1.12 μM. Penconazole and uniconazole inhibited MATE1 with IC 50 = 0.94 μM and 0.87 μM. Tebuconazole and hexaconazole inhibited MATE2-K with IC 50 = 0.96 μM and 1.04 μM, indicating that triazole fungicides may inhibit renal drug transporter activity at low concentrations. Triticonazole was transported by OAT1 and OAT3, and the K m values of triticonazole were 5.81 ± 1.75 and 47.35 ± 14.27, respectively. Tebuconazole and uniconazole were transported by OAT3, and the K m values of tebuconazole and uniconazole were 30.28 ± 7.18 and 87.61 ± 31.70, respectively, which may induce nephrotoxicity.
研究领域:
药理学
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毒理学
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分子生物学
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DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 6
·2611
·
IF 4.6
Hui Ye,Yuqin Luo,Yanjie Li,Xiangyun Wang,Hongmei He,Jinhua Jiang,Jianzhong Yu,Changpeng Zhang
A simple and sensitive method for the simultaneous quantitation of prohexadione-Ca and uniconazole in the field experiment ofOryza sativaL. andCitrus reticulataBlanco was established using solid-phase extraction (SPE) with polymer anion exchange (PAX) and Florisil followed by LC-MS/MS. The method demonstrated excellent linearity (R2> 0.999 0), trueness (recoveries between 95~105%), precision (CVs between 0.8~12%), sensitivity, and repeatability (LOQ of 0.05 and 0.01 mg/kg, respectively). Residue tests were conducted in the field at 12 representative sites in China, revealing final concentrations of prohexadione-Ca and uniconazole in brown rice, rice hull, and rice straw to be below 0.05 mg/kg, while in whole citrus fruit and citrus pulp, they were below 0.01 mg/kg. These were below the maximum residue limits specified in China. The chronic dietary risks of prohexadione-Ca and uniconazole in rice crops and citrus fruits were calculated to be 0.48% and 0.91%, respectively. Our research suggests that the chronic risk associated with the daily consumption of rice crops and citrus fruit at the recommended dosage is acceptable.Keywords:Oryza sativaL.;citrus fruit;dietary intake risk assessment;plant growth regulators
研究领域:
植物学
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农业科学
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食品安全
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）