RUSSIAN JOURNAL OF ORGANIC CHEMISTRY
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2024
·Vol. 60
·期 10
·1999-2003
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IF 0.8
Li C. L.,Wang S. Y.,Zhan M. R.,Chen C. C.,Wang Z. X.
Chiral monosubstituted cyclohexanediamine catalyst, namely (1 R ,2 R )-2-(piperidin-1-yl)cyclohexan-1-amine ( Cat1 ) has been efficiently synthesized and used to catalyze the aldol reaction of cyclohexanone and 2-(trifluoromethyl)benzaldehyde to obtain ( R )-2-{( S )-hydroxy[2-(trifluoromethyl)phenyl]methyl}cyclohexan-1-one. The catalyst (20 mol %) provided high yield (up to 85%) and excellent enantioselectivity (95.6% ee ) in water solution in three parallel tests.
研究领域:
有机化学
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催化化学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 2
·1-8
·
IF 5.3
Xu Kun,Dai Li,Du Yu,Liu Lei,Zhang Nuo,Feng Ruiqing,Xu Rui,Wei Qin
A photoelectrochemical (PEC) immunosensor was designed based on MgIn 2 S 4 -decorated inorganic halide perovskite CsPbBr 3 combined with the signal polarity conversion strategy for neuron-specific enolase (NSE) detection. CsPbBr 3 was applied as the basic photoactive material owing to its excellent optical and electronic properties, which provide a good PEC performance for sensor construction. In order to improve the stability of this perovskite, the three-dimensional flower-like MgIn 2 S 4 with a desirable direct band gap was applied to enhance the PEC response. Also, the excellent structure of MgIn 2 S 4 provides large surface-active sites for CsPbBr 3 loaded. For enhancing the detection sensitivity of PEC immunosensor, p-type CuInS 2 was used as a signal probe which fixed on detection antibody (Ab 2 ). When the target NSE was present, the photogenerated electrons produced by CuInS 2 were transferred to the test solution, and the polarity of PEC signal changes. Based on the above photosensitive materials and signal conversion strategy, the proposed PEC immunosensor showed favorable detection performance, and the linear detection range is 0.0001 ~ 100 ng/mL with a 38 fg/mL of detection limit. The proposed strategy improved the adhibition of CsPbBr 3 in the analytical chemistry field as well as provided a reference method for other protein detections. Graphical Abstract
CAS / MDL:
研究领域:
生物医学工程
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分析化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Weixiang Li,Di Shu,Hongliang Han,Zhanfang Ma
Nanogel as a multifunctional carrier was used to fabricate immunoprobe. The obtained immunoprobe was applied in a competitive amperometric immunosensor and exhibited an excellent analytical performance for the detection of tumor marker. The nanogel was easily fabricated by irradiation of the mixture of glucose, sodium citrate and sodium alginate by microwave, which exhibits excellent adsorbent ability for multivalence metal ions. The immunoprobe was constructed by functionalizing such uniform nanogels with multivalence metal ions (Cu 2+ as model) as redox species and gold nanoparticles for immobilizing antibody. The signal of Cu 2+ from the nanogel can be directly measured without acid dissolution and pre-concentration, which substantially simplified the detection steps. Besides, the response current can be amplified by glucose because Cu 2+ is an electro-catalyst for the oxidation of glucose. Polyaniline hydrogel with a three dimensional network structure and π–π conjugated structure was used to fabricate immunosensing substrate, which extremely improved the specific surface area and conductivity of the modified electrode. Under optimal conditions, the current changes of square wave voltammetry were used to detect neuron specific enolase (as a model analyte) with a broad linear ranging from 0.01 to 1000 ng mL −1 and a ultralow detection limit of 4.6 pg mL -1 (S/N = 3). This work provides a new strategy for designing multifunctional immunoprobes.
研究领域:
生物医学工程
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分析化学
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肿瘤学
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Xianyun Zheng,Ye Gao,Wanghong Li,Shu Wang
Propsilocerus akamusi (Tokunaga) is a common species of midge in Siberia , Japan, and China and an important prey species for fish and aquatic birds. Furthermore, this species has been shown to have an extraordinary capacity to resist cadmium (Cd) toxicity. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) coupled liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to analyze relative changes in the P. akamusi hemolymph proteome following exposure to a sublethal concentration of Cd 2+ . The results showed that Cd 2+ stress affects energy metabolism in P. akamusi . After examining the differentially expressed proteins (DEPs), only one up-regulated protein associated with metabolism, α-enolase (Eno1) was identified and further isolated and characterized. Sequence alignments showed that the deduced P. akamusi Eno1 amino acid sequence is highly conserved, with similarities of 77–95% noted when compared to other Dipteran Eno1 sequences. Furthermore, prolonged Cd 2+ exposure impacted Eno1 transcription, protein expression and enzyme activity levels. These results suggest that Eno1 may play a role in the response to Cd 2+ stress in P. akamusi .
研究领域:
环境毒理学
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蛋白质组学
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昆虫生理学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 42
·20146-20153
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IF 3.925
Zhengxing Gong,Jing Luo,Xinrong Shao,Xu Sun,Huan Wang,Dan Wu,Dawei Fan,Yuyang Li,Qin Wei,Huangxian Ju
A novel quenching electrochemiluminescence (ECL) immunosensor for the trace detection of neuron-specific enolase (NSE) was developed. Doping CdS with Zn2+ to obtain ZnCdS not only significantly improves the original properties of CdS, but also can be loaded onto the surface of g-C3N4 to form a special complex structure, which effectively improves the ECL performance of g-C3N4. Rh0.6Ru0.4 alloys with flower-like structures were prepared by simple hydrothermal synthesis, with excellent electrical conductivity and catalytic performance. Due to its special flower-like structure with a large specific surface area, Ag nanoparticles can be uniformly loaded on its surface to form Rh0.6Ru0.4@Ag composites. Through spectral comparison, it was found that Rh0.6Ru0.4@Ag has strong UV-vis absorption in the ECL emission wavelength range of g-C3N4, which can generate resonance energy transfer, thereby effectively quenching the ECL signal. The immunosensor has a wide detection range with a detection limit of 16 fg mL−1 (S/N = 3), good stability and biocompatibility, and has great potential in clinical application.
研究领域:
分析化学
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生物传感器
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生物医学工程
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材料科学
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DOI
高影响因子
ANALYST
·
2025
·Vol. 150
·期 5
·914-921
·
IF 3.3
Xianchen Hu,Yali Xu,Yuexi Lin,Xinghe Chen,Junshan Lin
Methods based on enzyme labelling strategies have been widely developed for capacitance immunoassays, but most suffer from low sensitivity and are unfavorable for routine use in the early stages of diagnostics. Herein, we designed a highly efficient capacitance immunosensing method for the low-abundance neuroblastoma biomarker neuron-specific enolase (NSE) using an interdigitated micro-comb electrode. Initially, monoclonal mouse anti-human NSE capture antibodies were immobilized on the interdigitated gold electrodes using bovine serum albumin. Thereafter, a sandwich-type immunoreaction was carried out in the presence of target NSE using horseradish peroxidase (HRP)-labeled secondary antibodies. The labelled HRP subsequently triggered the formation of tyramine–enzyme conjugate repeats with the help of HRP–tyramine and H2O2. The concatenated HRP molecules catalyzed the oxidation of 4-chloro-1-naphthol to produce an insoluble precipitate on the interdigitated micro-comb electrode, resulting in a shift in capacitance. Two protocols, with and without tyramine–HRP repeats, were investigated for the detection of NSE, with improved analytical performance achieved through tyramine signal amplification. Under optimum conditions, the interdigitated capacitance immunosensors exhibited good responses to target NSE within a dynamic linear range of 1.0–10 000 pg mL−1, with a low detection limit of 0.78 pg mL−1. An intermediate reproducibility of ≤9.67% was accomplished with batch-to-batch consistency, and good anti-interference capacity against other proteins was acquired. No significant differences at the 0.05 significance level were encountered in the analysis of 12 human serum specimens between the developed capacitance immunosensor and the commercially available enzyme-linked immunosorbent assay (ELISA).
研究领域:
医学诊断
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生物传感器
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肿瘤标志物检测
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DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 6
·3143-3150
·
IF 2.936
Ying Wang,Peng Zhao,Leilei Mao,Yajun Hou,Dawei Li
The development of rapid, highly sensitive detection methods for neuron-specific enolase (NSE) and S100-β protein is very important as the levels of NSE and S100-β protein in the blood are closely related to brain injury. Therefore, we can use NSE and S100-β protein concentration detection to realize the preliminary judgment of brain injury. In this paper, we report that a simple label-free three dimensional hierarchical plasmonic nano-architecture has been designed for the sensitive surface-enhanced Raman scattering immunosensor detection of NSE and S100-β. Owing to the active group of the hollow gold nanospheres (HAuNPs), the redox molecules 4-mercaptobenzoic acid (4-MBA) and Nile blue A (NBA) absorb antibodies and provide signal generation. The prepared HAuNPs@4-MBA and HAuNPs@NBA are used as probes to easily construct a surface-enhanced Raman scattering immunosensor. When protein biomarkers are present, the sandwich nanoparticles are captured over the substrate, forming a confined plasmonic field, leading to an enhanced electromagnetic field in intensity and in space. As a result, the Raman reporter molecules are exposed to a high density of “hot spots”, which remarkably amplify the Raman signal, improving the sensitivity of the surface-enhanced Raman scattering immunosensor. Under the optimized conditions, the linear range of the proposed immunosensor is from 0.2 to 22 ng mL−1 for both NSE and S100-β. The lowest detectable concentration is 0.1 and 0.06 ng mL−1 for NSE and S100-β, respectively. The assay results for serum samples with the proposed method were in a good agreement with the standard enzyme-linked immunosorbent assay method. The proposed immunosensor is promising in clinical diagnosis. This method, which utilizes the surface-enhanced Raman scattering of HAuNPs, has great potential in the detection of biomarkers, which are vital in medical diagnoses and disease monitoring.
研究领域:
医学诊断
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生物化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Cell Death & Disease
·
2024
·Vol. 15
·期 7
·1-18
·
IF 8.1
Wang Sikai,Cheng Hongxia,Li Miaomiao,Gao Dongmei,Wu Haoran,Zhang Shanshan,Huang Yilan,Guo Kun
An increasing evidence supports that cell competition, a vital selection and quality control mechanism in multicellular organisms, is involved in tumorigenesis and development; however, the mechanistic contributions to the association between cell competition and tumor drug resistance remain ill-defined. In our study, based on a contructed lenvitinib-resistant hepatocellular carcinoma (HCC) cells display obvious competitive growth dominance over sensitive cells through reprogramming energy metabolism. Mechanistically, the hyperactivation of BCL2 interacting protein3 (BNIP3) -mediated mitophagy in lenvatinib-resistant HCC cells promotes glycolytic flux via shifting energy production from mitochondrial oxidative phosphorylation to glycolysis, by regulating AMP-activated protein kinase (AMPK) -enolase 2 (ENO2) signaling, which perpetually maintaining lenvatinib-resistant HCC cells’ competitive advantage over sensitive HCC cells. Of note, BNIP3 inhibition significantly sensitized the anti-tumor efficacy of lenvatinib in HCC. Our findings emphasize a vital role for BNIP3-AMPK-ENO2 signaling in maintaining the competitive outcome of lenvitinib-resistant HCC cells via regulating energy metabolism reprogramming; meanwhile, this work recognizes BNIP3 as a promising target to overcome HCC drug resistance.
研究领域:
癌症生物学
·
分子生物学
·
细胞生物学
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药理学
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DOI
PubMed
高影响因子
Microbiology Spectrum
·
2022
·Vol. 10
·期 4
·
IF 9.043
Liping Li,Hui Lu,Xuan Zhang,Malcolm Whiteway,Hao Wu,Shanlun Tan,Jianye Zang,Shujuan Tian,Cheng Zhen,Xianlei Meng,Wanqian Li,Dazhi Zhang,Min Zhang,Yuanying Jiang
Baicalein (BE) is a promising antifungal small-molecule compound with an extended antifungal spectrum, good synergy with fluconazole, and low toxicity, but its target protein and antifungal mechanism remain elusive. In this study, we found that BE can function against Candida albicans by disrupting glycolysis through targeting Eno1 and inhibiting its function. Eno1 acts as a key therapeutic target of the drug, as BE had no antifungal activity against the eno1 null mutant in a Galleria mellonella model of C. albicans infection. To investigate the mechanism of action, we solved the crystal structure of C. albicans Eno1(CaEno1) and then compared the difference between this structure and that of Eno1 from humans. The predicted primary binding site of BE on CaEno1 is between amino acids D261 and W274, with D263, S269, and K273 playing critical roles in the interaction with BE. Both positions S269 and K273 have different residues in the human Eno1 (hEno1). This finding suggests that BE may bind selectively to CaEno1, which would limit the potential for side effects in humans. Our findings demonstrate that Eno1 is a target protein of BE and thus may serve as a novel target for the development of antifungal therapeutics acting through the inhibition of glycolysis.IMPORTANCE Baicalein (BE) is a promising antifungal agent which has been well characterized, but its target protein is still undiscovered. The protein Eno1 plays a crucial role in the survival of Candida albicans. However, there are few antifungal agents which inhibit the functions of Eno1. Here, we found that BE can function against Candida albicans by disrupting glycolysis through targeting Eno1 and inhibiting its function. We further solved the crystal structure of C. albicans Eno1(CaEno1) and predicted that the primary binding site of BE on CaEno1 is between amino acids D261 and W274, with D263, S269, and K273 playing critical roles in the interaction with BE. Our findings will be helpful to get specific small-molecule inhibitors of CaEno1 and open the way for the development of new antifungal therapeutics targeted at inhibiting glycolysis.
研究领域:
微生物学
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药理学
·
分子生物学
·
结构生物学
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DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2017
·
IF 2.334
He Li,Qiyou Xiao,Jiaxin Lv,Qin Lei,Yujie Huang
In this work, three-dimensional (3D) hyperbranched TiO 2 nanorod arrays were synthesized and used to fabricate dopamine sensitized photoelectrochemical (PEC) biosensor. To increase the lifetime of charge carriers and enhance the photocurrent responses signal, a delicate signal amplification strategy by introducing dopamine (DA) as sensitizer was developed. The dopamine sensitized TiO 2 can shorten the carrier diffusion distance, improve light harvesting efficiency and charge collection efficiency, which results in performance improvement of the as-obtained PEC sensor. This proposed biosensor for determination of neuron specific enolase (NSE) demonstrated a good linear relationship range from 0.1 ng mL −1 to 1000 ng mL −1 with a detection limit of 0.05 ngmL −1 (S/N = 3). In addition, the as-prepared immunosensor exhibits excellent selectivity , stability and reproducibility, which could be extended to other label-free sensing fields. Therefore, this proposed method may also provide potential applications for the clinical examination.
研究领域:
纳米技术
·
生物传感器
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 43
·期 15
·5925-5931
·
IF 3.201
Dawei Li,Mingfeng Yang,Hanxia Li,Leilei Mao,Ying Wang,Baoliang Sun
An inexpensive and disposable lateral flow glass-hemostix (FGH) has been developed as an immunoassay, in which surface-enhanced Raman scattering (SERS) is utilized for sensing signal transduction. Gold nanocage@Raman reporter nanoparticles are developed as SERS tags, which is the key to the high sensitivity of the device. Compared with ELISA, the SERS-FGH exhibits superior performance in terms of sensitivity and limit of detection (LOD) in a blood plasma-containing sample matrix. In addition, the SERS-FGH has been successfully used for the detection of neuron-specific enolase (NSE), a traumatic brain injury (TBI) protein biomarker, in diluted blood plasma samples, achieving a LOD of 0.74 ng mL−1. Moreover, the SERS-FGH was successfully employed to measure the NSE level in clinical blood plasma samples taken from identified TBI patients. This work demonstrates that the SERS-FGH has great potential in assisting screening of TBI patients in the point-of-care setting.
研究领域:
医学诊断
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生物传感
·
纳米技术
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Jiayi Miao,Yifan Liu,Yi Xiao,Congzheng Yuan,Qian Xu,Panpan Chen,Yang Jin,Liying Zhang,Hongliang He,Shuhu Du
Perovskite/metallic heterojunction-based surface enhanced Raman scattering (SERS) substrates have been proven to be capable of providing Raman enhancement. However, the inherent water instability and poor dispersibility of perovskite/metallic nanocomposites-based SERS substrates pose significant challenges to their application in aqueous environments. Herein, polydopamine (PDA)-encapsulated cesium lead bromide (CsPbBr 3 ) adsorbing gold nanoparticles (AuNPs), termed as CsPbBr 3 @PDA@AuNPs, is prepared as SERS substrate, which exhibits excellent water stability and SERS activity. Dopamine as organic ligand not only passivates surface defects during the growth of perovskite nanocrystals, but also forms porous PDA protective layer, effectively preventing degradation of perovskite in aqueous medium. Meanwhile, PDA with abundant functional groups and conjugated π structure will adsorb AuNPs and promote electron flow between CsPbBr 3 and AuNPs, resulting in strong SERS activity. Based on the results, a SERS aptasensor has been fabricated by conjugation between CsPbBr 3 @PDA@AuNPs and double-stranded DNA (dsDNA), which is composed of neuron-specific enolase (NSE) aptamer and partial complementary signal-stranded DNA (ssDNA). The working strategy of as-fabricated SERS aptasensor is based on the conformational change (of ssDNA)-triggered Raman response for the detection of NSE. Upon the addition of NSE, the specific binding of NSE aptamers to NSE can convert rigid dsDNA into a flexible ssDNA, and the Cy5 signal molecule modified at the end of ssDNA will close to CsPbBr 3 @PDA@AuNPs SERS substrate, generating significant Raman signals with the lower limit of detection (1.02 pg/mL) of NSE. The SERS aptasensor has broad application prospect in the field of life/medicine science fields (e.g. early diagnosis and screening of disease).
研究领域:
生物医学
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分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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