Analytical Methods
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2022
·Vol. 14
·期 11
·1140-1147
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IF 3.532
Tuqiang Li,Yulan Wang,Yanan Zhang,Guobao Zhou,Lei Li
An entropy-driven DNA circuit offers an efficient means of sensitive analyte detection with signal amplification. In this article, we rationally engineered an aptamer-based entropy-driven signal-off DNA circuit for colorimetric detection of small molecules. The proposed signal-off DNA circuit is activated by target small molecule binding to drive the collapse of G-quadruplex DNAzyme, accompanied by the colour change of the detection solution from dark blue to light blue. Entropy-driven recycling hybridization significantly magnified the input signal of the target small molecule. Such an assay enables naked-eye detection of adenosine triphosphate and oxytetracycline at concentrations as low as 0.5 μM and 1 μM respectively. Moreover, when compared with the signal-on DNA circuit, the entropy-driven signal-off DNA circuit for colorimetric detection has two advantages. Firstly, unlike in the signal-on DNA circuit, the unavoidable formation of waste complexes in the absence of a target in the signal-off DNA circuit has no influence on target detection performance as its background signal is only determined by the substrate complex. Secondly, the signal-on DNA circuit cannot distinguish false-positive signals generated by invasive catalysts (e.g., HRP, serum, Fe3O4), while the signal-off DNA circuit can distinguish those signals as undesired signals. Overall, the signal-off DNA circuit affords a novel strategy for sensitive and accurate detection of small molecules.
研究领域:
生物化学
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分子生物学
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DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2024
·
IF 4.8
Longjian Huang,Wenzhao Zhang,Mingxuan Liu,Yuanxun Gong,Qianli Tang,Kaihua Wang,Xianjiu Liao,Kai Zhang,Jihua Wei
We present an innovative biosensor designed for the precise identification of Escherichia coli ( E.coli ), a predominant pathogen responsible for gastrointestinal infections. E.coli is prevalent in environments characterized by substandard water quality and can lead to severe diarrhea, especially in hospital settings. The device employs entropy-driven reactions to synthesize copious amounts of double-stranded DNA (dsDNA), which, upon binding with crRNA, triggers the CRISPR/Cas12a system’s cleavage mechanism. This process results in the separation of a ferrocene (Fc)-tagged DNA strand from the electrode, enhancing the electrochemical signal for E.coli ’s rapid and accurate detection. Our tests confirm the biosensor’s ability to quantify E.coli across a dynamic range from 100 to 10 million CFU/mL, achieving a detection threshold of just over 5 CFU/mL. The development of this electrochemical biosensor highlights its exceptional selectivity, high sensitivity, and user-friendly interface for E.coli detection. It stands as a significant step forward in pathogen detection technology, promising new directions for identifying various bacterial infections through the CRISPR/Cas mechanism.
研究领域:
分子生物学
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病原体检测
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生物传感技术
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DOI
PubMed
高影响因子
Materials Horizons
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2018
·Vol. 6
·期 3
·538-545
·
IF 13.183
Zhouyue Lei,Peiyi Wu
A novel type of adaptable polyionic elastomers with rational molecular design is reported to address the dilemma encountered in soft electronic and ionic conductors. The conductivity of these elastomers is stable both during stretching and in air. The polyionic elastomers are highly transparent, 3D-printable, ultra-stretchable, self-healable, self-powered, and capable of sensing strain, stress, touch, humidity, temperature, etc. In addition, they can mimicking organisms with entropy-driven actuation and feedback. This type of materials contributes to prosthetic skins and neuromuscular systems and shows great potential for soft robotics and artificially intelligent applications.
研究领域:
材料科学
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生物医学工程
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人工智能
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DOI
高影响因子
THERMOCHIMICA ACTA
·
2025
·
IF 3.5
Jixiu Deng,Pengshuai Zhang,Han Sun,Wang Rongrong,Binbin Wu,Yuemei Li,Beibei Feng,Haowei Sun,Shuoye Yang
This study aimed to investigate the solubility and dissolution thermodynamics of pregnenolone (Form I) in n -propanol, N-Methyl-2-pyrrolidone (NMP), ethyl acetate, 2-methoxyethanol, ethyl formate, n -butanol, 2-butoxyethanol, N, N-Dimethylformamide (DMF), 2-propoxyethanol, i -propanol, ethanol, 2-ethoxyethanol and methanol from 283.15 K to 323.15 K by a laser dynamic method. The experimental solubility of pregnenolone (Form I) was successfully fitted using five thermodynamic models (Jouyban, Apelblat, NRTL, van't Hoff, UNIQUAC), and the van't Hoff equation provided the best prediction effect with a minimum average RAD (relative average deviation) of 1.00 × 10 -2 and RMSD (root mean square deviation) of 6.58 × 10 -5 . Hansen solubility parameter (HSPs) was used to evaluate the dissolution behavior including the solubility order of pregnenolone (Form I). The apparent thermodynamics were evaluated, and results showed that entropy was the main driving force during the dissolution process of pregnenolone (Form I).
研究领域:
药学
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物理化学
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DOI
高影响因子
Journal of Energy Storage
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2025
·
IF 9.8
Hao Zheng,Yaohang Gu,Kailong Ma,Xiaoyan Zhang,Xiwei Qi
High-performance dielectric capacitors are essential due to their exceptionally high instantaneous power density and rapid charge-discharge rates. To date, lead-free ceramic capacitors still face the challenge of low energy storage density. In this study, an entropy-driven optimization strategy was employed to enhance energy storage performance. As a result, the manipulation of configurational entropy in 0.62Sr 0.7 Bi 0.2 TiO 3 –0.38K 0.5 Bi 0.5 TiO 3 compounds (KBT-SBT) is realized by adding different amounts of Re 2 O 3 (where Re = La, Nd, Sm). Entropy-driven effects inhibit grain growth and, at the same time, limit the development of ferroelectric domains. Accordingly, the refinement of ferroelectric domains not only increased the breakdown field strength but also improved the energy storage efficiency. Moreover, doping KBT-SBT with 1 % (La 2/3 Nd 2/3 Sm 2/3 )O 3 exhibits the optimal energy storage performance. Which the material demonstrates an impressive energy density of 4.07 J/cm 3 with the efficiency of 79.75 % when applying an electric field strength of 230 kV·cm −1 . Interestingly, at an electric field strength of 100 kV·cm −1 , it demonstrated exceptional stability across temperature, frequency, and fatigue tests. The outstanding performance of KBT-SBT based ceramics proves that our entropy-driven strategy opens a new way to understand and design dielectric energy storage materials.
研究领域:
材料科学
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能源存储
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Ping Qiu,Longjian Huang,Jiayi Zhang,Wenbo Li,Xudong Xin,Luchang Chen,Qianli Tang,Kai Zhang,Xianjiu Liao,Jihua Wei
We report the development of a novel split aptamer-driven electrochemical biosensor integrating entropy-driven Signal amplification (EDSA) and CRISPR-Cas12a technology for the highly sensitive and specific detection of adenosine deaminase (ADA). ADA is an important biomarker involved in immune regulation and purine metabolism, and its accurate quantification is critical for diagnosing various diseases. In our system, ADA catalyzes the deamination of adenosine to inosine, resulting in the release of Aptamer1 from a split aptamer structure. The liberated Aptamer1 initiates a strand displacement reaction within a Blocker/Bandage/Scaffold complex, triggering a cascade of entropy-driven amplification cycles that generate a DNA duplex capable of activating CRISPR-Cas12a. Activated Cas12a exhibits trans -cleavage activity, specifically cleaving a ferrocene-labeled single-stranded DNA (ssDNA) probe immobilized on a gold electrode, leading to a measurable decrease in electrochemical signal. The biosensor operates under isothermal and enzyme-free conditions, providing simplicity, rapid detection, and low cost. It achieves a low detection limit of 0.688 U·L −1 with a wide linear range from 1 to 20,000 U·L −1 . The system demonstrates excellent specificity against ADA analogues and common interfering proteins and performs reliably in diluted human serum samples. This strategy represents a versatile and modular platform for point-of-care diagnostics. By altering the aptamer sequence, the biosensor can be adapted for the detection of other clinically relevant proteins and enzymes, contributing to advances in personalized medicine.
研究领域:
生物传感技术
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分子诊断
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医学检测
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DOI
高影响因子
Analytical Methods
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2024
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IF 2.7
Na Qin,Sunlei Yang,Rui Li,Hongyun Zhan,Lijuan Wang,Fengyun Li,Jingbo Liu
The traditional method of acetamiprid residue detection is difficult to operate, time-consuming and laborious, and requires high professional knowledge of the detection personnel, which can no longer meet the rapid detection in the field. For this reason, a colorimetric aptasensor based on an entropy-catalyzed amplification system was developed for the ultrasensitive and selective determination of acetamiprid. In the absence of acetamiprid, the aptamer and cDNA build a double-stranded structure. The formed hemin/G-quadruplex mimicking DNAzyme can catalyze the substrate ABTS to generate the colored ion ABTS- with the help of H2O2, and the solution turns blue-green. On the contrary, the presence of acetamiprid triggers the release of cDNA, which in turn initiates the entropy-driven system, resulting in the inability to form DNAzyme and therefore no blue-green production of the solution. The quantity of acetamiprid determined the color period. Under the optimal experimental conditions, the method showed a linear correlation (R2=0.9837) for the detection of acetamiprid over the concentration range of 0.1 ~ 100 ng/mL, with a limit of detection of 0.06 ng/mL.The method was used for the determination of acetamiprid in spiked Coix lacryma and bitter almond, with the recoveries in the range of 90.3% ~ 110.3%. The proposed enzyme-free and label-free assay can be developed into a simple, sensitive and rapid detection platform.
研究领域:
分析化学
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环境科学
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生物技术
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DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Haixiang Li,Shuo Lian,Zhiwei Zhang,Weiye Bi,Qingyou Meng,Qingwei Ding
Brain natriuretic peptide (BNP) is considered a reliable marker of heart failure disease, and its timely detection can provide important pathological information to prevent or treat heart failure. In this article, an electrochemiluminescence (ECL) sensing device based on a boron carbon nitride/gold nanoparticle (BCN/AuNPs) complex is developed to determine BNP. Prominently, the CRISPR/CAS 13a enzyme reverse cleavage mode, the entropy-driven and hammer hybridization chain reaction processes were involved in the entire detection scheme. Ultimately, with multiple reaction methods and amplification reactions of nucleic acids, this ECL sensing device is able to achieve a detection limit as low as of 0.03 pg/mL and linear range from 0.1 pg/mL to 30 ng/mL for BNP. In addition, the ECL sensing device based on BCN/AuNPs complex obtained satisfactory stability and specificity, and can also be extended to the detection of other pathological markers.
研究领域:
生物医学工程
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分子诊断学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2012
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IF 3.696
Jingjing Jiao,Bin Dong,Huina Zhang,Yingyuan Zhao,Xiaoqing Wang,Rui Wang,Li Yu
Halogen-free, low-cost alkyl sulfate-based surface active ionic liquids (SAILs), 1-butyl-3-methylimidazolium dodecyl sulfate ([C4mim][C12SO4]), and N-butyl-N-methylpyrrolidinium dodecyl sulfate ([C4MP][C12SO4]) were easily synthesized through ion exchange reaction. The aggregation behaviors of [C4mim][C12SO4] and [C4MP][C12SO4] in aqueous solution were investigated by surface tension, electric conductivity, and static fluorescence quenching. Both [C4mim][C12SO4] and [C4MP][C12SO4] have rather lower cmc, γcmc values and higher pC20, πcmc values than those reported for the traditional ionic surfactant, sodium dodecyl sulfate (SDS), and imidazolium-based SAIL, 1-dodecyl-3-methylimidazolium bromide ([C12mim]Br), with the same hydrocarbon chain length. The thermodynamic parameters evaluated from electric conductivity measurements show that the micelle formation of [C4mim][C12SO4] and [C4MP][C12SO4] is entropy-driven in the temperature range investigated. Lower average aggregation number indicates that the micelles of two SAILs present much looser structure. It is found that both the nature and the ring type of counterions can affect the aggregation behavior in aqueous solution. 1H NMR results of [C4mim][C12SO4] were used to further verify the mechanism of micelle formation. Hydration ability and steric hindrance of the imidazolium or pyrrolidinium counterion as well as the cooperative hydrophobic interaction of longer alkyl chain of [C12SO4] anion and comparatively shorter alkyl chain of [C4mim] or [C4MP] cation are proposed to play critical roles in the aggregation of [C4mim][C12SO4] and [C4MP][C12SO4].
研究领域:
化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2021
·Vol. 146
·期 5
·1668-1674
·
IF 4.616
Shasha Li,Hong-Min Meng,Hong Zong,Juan Chen,Jianjun Li,Lin Zhang,Zhaohui Li
Accurate, sensitive and rapid nucleic acid tests are important to implement timely treatment measures and control the spread of disease. Herein, we developed a novel portable platform for highly sensitive and specific detection of nucleic acids by integrating an entropy-driven amplification strategy into lateral flow assay (LFA) biosensor. We find that introducing an entropy-driven amplification strategy yields bright intensities on the test line of LFA stirp, which results in improved sensitivity for targeted nucleic acid detection. The developed LFA biosensor showed good reproducibility, specificity and sensitivity for target DNA and H1N1-RNA detection with a low detection limit of 1.43 pM and 2.02 pM, respectively. Its practical potential was also verified by detecting the target nucleic acid in human serum. More importantly, the design of an entropy-driven amplification strategy in this portable platform retained the convenient, rapid and low-cost characterizations of LFA biosensor due to the compact amplification principle and the elimination of enzyme use. Thus, we believe that this assay biosensor will certainly report its own position in the timely detection of nucleic acid, especially when the medical environment and resources are fewer.
研究领域:
分子生物学
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诊断学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Jing Wang,Lu Shi,Xinyu Zhu,Qiaorong Tang,Mengmeng Wu,Baoxin Li,Wei Liu,Yan Jin
Alzheimer's disease (AD) is a degenerative disease of the brain worldwide. Currently, there is no effective cure. But accurate and early diagnosis of AD is critical to the development of patient care and future treatments. MiRNA-16 has been considered as an effective diagnostic biomarker for AD because of its regulatory effect on key proteins of AD. Herein, a colorimetric lateral flow assay (LFA) was developed for sensitive detection of miRNA-16 based on entropy-driven catalysis (EDC) amplification strategy. MiRNA-16 triggered EDC and released more linker DNAs (LDNA) of sandwich structure. Thus, AuNPs were enriched at the T-line to enhance the colorimetric signal and improve the sensitivity of visual assay. It showed good specificity and sensitivity for detecting miRNA-16 with a detection limit of 1.01 pM. The practical detection of miRNA-16 in human serum obtained satisfactory result. Significantly, EDC achieved signal amplification in homogeneous solution without enzyme and DNA labeling, leading to a cheap and easy detection of miRNA-16. Therefore, it provided a portable and rapid assay for AD-related nucleic acid , which holds a potential for point-of-care testing (POCT) of AD.
研究领域:
生物化学
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医学诊断
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分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Wenshan Li,Qingqing Li,Huaiyue Xia,Wenjie Liu,Guoxing Jing,Jianna Yu,Wen Liu,Mengtong Lin,Yujuan Huang
Ochratoxin A (OTA) is a compound of concern due to its potential health effects on humans. Detecting OTA in food is crucial for safeguarding public health. In this study, we fabricated a multi-DNAzyme cascade reaction-mediated colorimetric aptasensors for OTA detection, integrating autocatalytic Mg 2+ -dependent DNAzyme cleavage (MNAzyme) and an entropy-driven circuit. In brief, the recognition between the aptamer and target OTA led to the release of DNA1. Subsequently, DNA1 hybridized with DNA2, generating an upstream MNAzyme that facilitated the production of a downstream MNAzyme. These MNAzymes possess similar substrate binding arms, enabling them to catalyze the same substrate. The catalytic efficiency of MNAzymes towards the substrates was enhanced due to the increasing concentration of MNAzymes. The cleavage products then triggered an entropy-driven cycle to generate a signal. Under optimal conditions, the sensing system exhibited low detection limits of 48.97 fM for OTA. Additionally, the proposed aptasensor was successfully applied to quantitatively analyze OTA in food samples. Thus, the multi-DNAzyme cascade reaction-mediated colorimetric aptasensors offer an adaptable platform for detecting traces of OTA contaminant in food.
研究领域:
食品安全检测
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）