JOURNAL OF RARE EARTHS
·
2024
·
IF 5.2
Chunqing Fu,Wei Meng,Xiaoyan Fu,Shuya Wang,Wenzhi Sun,Hongwu Zhang
X-ray irradiated La 2 Ti 2 O 7 :Pr 3+ was found to show long-time sustainable mechanoluminescence (ML), which can realize the high sensitivity and real time detection of stress burst for more than 72 h. Under the excitation of X-ray, La 2 Ti 2 O 7 :Pr 3+ shows red ML emissions located at 610, 624 and 638 nm, which are similar to the afterglow emission spectrum and can be ascribed to 1 D 2 - 3 H 4 , 3 P 0 - 3 H 4 and 3 P 0 - 3 H 2 of Pr 3+ . And the linear increases of compressive load can induce the linear increases of ML intensity of the sample, indicating that the X-ray induced ML emission can be utilized to accurately detect the stress applied on the object. Furthermore, X-ray irradiated La 2 Ti 2 O 7 :Pr 3+ possesses excellent stability and the repeatability of ML emission and the ML signals can keep stable even 50 load cycles after 1 h decay. The thermoluminescence results suggest that abundant electrons are trapped in shallow and deep answered for stable ML emission. More importantly, the deeply trapped electrons induced ML emissions from X-ray irradiated La 2 Ti 2 O 7 :Pr 3+ can be utilized to detect the stress bursts for a long time. When the sample is recharged by 5 min X-ray irradiation once, within 24 h decay, the 1500 N burst among many 1000 N cycles can be clearly detected, and the signal to noise ratio (S/N) reaches 1.32 and 1.26 after 12 and 24 h decay, respectively. And even after 72 h decay, the S/N of 2500 N still arrives at 1.23 and that of 4000 N reaches 1.97, indicative of long-time high sensitivity detection of abnormal stress. All these results suggest that the X-ray-irradiated La 1.97 Ti 2 O 7 :0.03Pr 3+ /resin sample can realize the high sensitivity and real time detection of stress burst for more than 72 h, which thus possesses great potential application in early warning of the emergency disasters such as bridge fracture, tunnel collapse and so on.
研究领域:
材料科学
·
光学
·
物理学
✓ 已验证
DOI
高影响因子
MATERIALS LETTERS
·
2024
·
IF 2.7
Sicong Tu,Shuting Mai,Dan Shu,Yuxin Huang,Zhihong Nie,Yang Wang,Wuli Yang
It is challenging for the manufacturing of microspheres loaded with peptide due to the complicated preparation process and the issue of burst release. In this construct, a microfluidic flow-focusing device is developed to prepare and optimize poly (lactic- co -glycolic acid) (PLGA) Exenatide −loaded microspheres. Surfactants was added to greatly improve the stability of the emulsion, leading to increase drug loading capacity. By increasing the particle size of the drug-loaded microspheres to 30 μm, the burst release was significantly reduced. Additionally, multi-channel droplet microfluidic devices are prepared with the capability to achieve a 100-fold increase in throughput, which could achieve a production rate of 2 g per hour.
研究领域:
药剂学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Jingsong Lu,Zhenhu Guo,Wensheng Xie,Yongjie Chi,Wanling Xu,Xiaoxiao Guo,Xiaohan Gao,Jielin Ye,Benhua Xu,Rong Zheng,Xiaodan Sun,Xiumei Wang,Shenglei Che,Jing Yu,Lingyun Zhao
For the purpose of improving the quality of life and minimizing the psychological morbidity of a mastectomy, breast-conserving treatment (BCT) has become the more preferable choice in breast cancer patients. Meanwhile, tumor hypoxia has been increasingly recognized as a major deleterious factor in cancer therapies. In the current study, a novel, effective, and noninvasive magnetothermodynamic strategy based on an oxygen-independent free-radical burst for hypoxia-overcoming BCT is proposed. Radical precursor (AIPH) and iron oxide nanoparticles (IONPs) are coincorporated within the alginate (ALG) hydrogel, which is formed in situ within the tumor tissue by leveraging the cross-linking effect induced by the local physiological Ca2+ with ALG solution. Inductive heating is mediated by IONPs under AMF exposure, and consequently, regardless of the tumor hypoxia condition, a local free-radical burst is achieved by thermal decomposition of AIPH via AMF responsivity. The combination of magnetic hyperthermia and oxygen-irrelevant free-radical production effectively enhances the in vitro cytotoxic effect and also remarkably inhibits tumor proliferation. This study provides a valuable protocol for an hypoxia-overcoming strategy and also an alternative formulation candidate for noninvasive BCT.
研究领域:
肿瘤学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 19
·11578
·
IF 6.208
Bin Liu,Ming Tang,Hui Chen
Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (DaCYP4BQ1) is associated with the response of (+)-α-pinene inDendroctonus armandi. However, the regulatory mechanism of this P450 gene response to (+)-α-pinene is still unknown. In this study, spatiotemporal expression profiling indicated thatCYP4BQ1was highly expressed in adult and larval stages ofD. armandi, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression ofCYP4BQ1significantly increased after exposure to (+)-α-pinene, and depletion of it decreased the tolerance of adults to (+)-α-pinene. In addition, (+)-α-pinene treatment induced the expression of the transcription factors cap ‘n’ collar isoform C (CncC) and its binding factor muscle aponeurosis fibromatosis (Maf), elevated the level of hydrogen peroxide (H2O2), and increased the activities of antioxidant enzymes. SilencingCncCsuppressedCYP4BQ1expression and enhanced the susceptibility of beetles to (+)-α-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H2O2, suppressed the expression ofCncC, Maf, andCYP4BQ1and led to decreased tolerance of adults to (+)-α-pinene. In contrast, ingestion of the CncC agonist curcumin elevatedCYP4BQ1expression and enhanced (+)-α-pinene tolerance. The results demonstrate that, inD. armandi, (+)-α-pinene inducesCYP4BQ1via activation of the ROS/CncC signaling pathway.Keywords:Dendroctonus armandi;phytochemical tolerance;cytochrome P450;oxidative burst;CncC pathway
研究领域:
昆虫学
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Oxidative Medicine and Cellular Longevity
·
2019
·
IF 4.868
Qiaojian Zhang,Shengchen Wang,Shufang Zheng,Ziwei Zhang,Shiwen Xu
Neutrophil extracellular traps (NETs) are reticular structures formed by myeloperoxidase (MPO), histones, and neutrophil elastase (NE) that are released from neutrophils in response to pathogenic stimuli. Chlorpyrifos (CPF) is wildly used as an organophosphorus pesticide that causes a range of toxicological and environmental problems. Exposure to CPF can increase the production of neutrophils in carps, and this increase can be considered a biomarker of water pollution. To explore a relationship between NETs and CPF and its mechanism of influence, we treated neutrophils from the blood of carp with 1 μg/mL phorbol 12-myristate 13-acetate (PMA), 0.325 mg/L CPF, or 20 μM necrostatin-1 (Nec-1). The production of MPO and NETs was reduced in the CPF+PMA group compared with that in the PMA group. CPF can cause an increase in reactive oxygen species (ROS), while inhibiting respiratory burst caused by PMA stimulation. We found that the expression levels of protein-coupled receptor 84 (gpr84), dystroglycan (DAG), proto-oncogene serine/threonine kinase (RAF), protein kinase C (PKC), and mitogen-activated protein kinase 3 (MAPK3) in the CPF+PMA group were lower than those in the PMA group, indicating that the PKC-MAPK pathway was suppressed. The expression levels of cylindromatosis (CYLD), mixed lineage kinase domain-like pseudokinase (MLKL), receptor-interacting serine-threonine kinase 1 (RIP1), and receptor-interacting serine-threonine kinase 3 (RIP3) were increased, and the expression levels of caspase 8 were reduced by CPF, indicating that CPF may cause necroptosis. The addition of Nec-1 restored the number of NETs in the CPF+PMA group. The results indicate that CPF reduced the production of NETs by inhibiting respiratory burst and increasing necroptosis. The results contribute to the understanding of the immunotoxicological mechanism of CPF and provide a reference for comparative medical studies.
研究领域:
毒理学
·
免疫学
·
环境科学
·
水生生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Chemistry Frontiers
·
2021
·Vol. 5
·期 16
·6027-6040
·
IF 6.482
Jingjing Jiang,Jingcheng Xiao,Zexu Zhao,Mao-Sen Yuan,Jinyi Wang
Orally targeted drug delivery for treating colonic diseases has attracted much attention because this method can be used as a better alternative to conventional administration of high drug doses, which usually exposes patients to serious systemic toxicity. Here, a pH-responsive nano-in-micro composite as a drug-delivery vehicle for the targeted therapy of inflammatory bowel disease (IBD) is designed and prepared using a one-step droplet microfluidic method with a water-in-oil-in-water manner. The microcapsules, encapsulating both free drugs and drug-loaded nanoparticles (NPs), appear as bowl-shaped spheres with a non-uniform shell thickness. Under colonic pH, the thin concave lamella of the HPMCAS-based shells rapidly ruptures and releases the inner free drug to achieve burst drug release for a sufficiently high and effective concentration of the drug. Subsequently, the gradual decomposition of PLGA-based drug-loaded NPs results in sustained drug release. The sequential burst–sustained drug release is consistent with the pharmacokinetics and medication needs of IBD. In vitro inflammation inhibition experiments further reveal that the nano-in-micro microcapsules can quickly alleviate the inflammatory response at the site of inflammation by reducing the intracellular ROS level and the expression of proinflammatory cytokines. These results demonstrate the success of the targeted drug-delivery model with the function of sequential burst–sustained drug release for IBD therapy.
研究领域:
药剂学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Nature Plants
·
2025
·
IF 13.6
Chen Xiuxiu,Jin Songsong,Du Hong,Liu Zebin,Hou Congcong,Tang Ling,Wang Yuanyuan,Wang Yuxi,Yang Zhaolin,Zhao Tong,Ma Jinying,Zhang Lanjun,Li Meijing,Qi Dongdong,Li Wenlong,Wang Xiaohua,Sun Runze,Moore John P.,Liu Yongxiu,Lin Jinxing,Deng Xin
Autophagy and apoplastic ascorbic acid (AsA) play important roles in plant drought tolerance. However, the trans-plasma membrane transport of AsA and its association with autophagy during drought stress are unclear. Here we report an AsA-induced autophagy pathway in plants, wherein plasma-membrane-located cytochrome b561 and DOMON domain (CYBDOM) proteins play positive roles. CYBDOM proteins from the resurrection plant Boea hygrometrica (BhDB) and Arabidopsis (AtDB1) can transport electrons across the plasma membrane using intracellular AsA as an electron donor and apoplastic monodehydroascorbic acid or Fe 3+ as electron acceptors in Xenopus laevis oocytes. Increased apoplastic AsA, autophagy and drought tolerance are observed in BhDB - and AtDB1 -overexpressing Arabidopsis compared with the wild type. CYBDOM proteins interact with respiratory burst oxidase homologue D (RbohD), which serves as the adaptor to bind autophagy related gene 8 protein (ATG8) and cargo protein for autophagic degradation. AsA increases AtDB1 protein level and its interaction with RbohD. Together, the AsA-activated CYBDOM–RbohD synergy to induce autophagy suggests a novel mechanism in plant drought and desiccation tolerance.
研究领域:
植物学
·
植物生理学
·
分子生物学
·
应激生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Qiyi Zhu,Xiaofeng Zhu,Ning Yang,Yuxi Duan,Yuanyuan Wang,Xiaoyu Liu,Di Zhao,Haiyan Fan,Lijie Chen
Root-knot nematodes ( Meloidogyne incognita ) present a significant threat to global agriculture, and the development of multi-drug resistance in these nematodes exacerbates this problem. Benzothiazole, a heterocyclic compound has been reported as a potential nematicide, however, its mode of action is not fully understood. This study aims to elucidate the nematicidal mechanism of benzothiazole against M. incognita . In the toxicity assays, benzothiazole exhibited rapid and effective nematocidal activity, significantly compromising egg masses and inhibiting egg hatching while killing newly hatched second-stage juveniles (J2s) of M. incognita . Microscopic observations revealed that after 48 h of incubation, a marked reduction in protein and carbohydrate levels within the J2s was observed. Notably, benzothiazole at a concentration of 14 mmol/L significantly inhibits glutathione S-transferase (GST) enzyme activity, leading to the accumulation of reactive oxygen species (ROS), ultimately resulting in rapid nematode death. Molecular docking and dynamics simulations demonstrated that benzothiazole forms a stable complex with GST, thereby disrupting its antioxidant function. Furthermore, in pot experiments, benzothiazole effectively reduced the gall formations of M. incognita on tomato roots. Overall, this novel inhibitory mechanism of glutathione S-transferase (GST) differs from that of the neurotoxicant abamectin, which targets glutamate-gated chloride channels (GluCl). This mechanism holds significant promise for the development of environmentally friendly nematicides. It offers a potential solution to the growing problem of multidrug resistance in root-knot nematodes and could help mitigate the substantial economic losses caused by these pests in global agriculture.
研究领域:
农业科学
·
害虫防治
·
生物化学
·
线虫学
·
植物病理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Lu Zhang,Fangyuan Chai,Ke Yan,Xiaojun Ning,Shuang Min,Lijian Guo,Yi Liu,Tao Gao,Tian Gao,Linli Du
Mitochondria have emerged as a promising therapeutic target in oncology due to their central regulatory roles in cellular homeostasis and apoptotic pathways. Herein, we present a rationally designed mitochondrial-targeting agent, phenolic thiazide -BODIPY (PBD), engineered through strategic conjugation of a BODIPY-pyridinium fluorophore with a phenolic thiazide pharmacophore. This novel theranostic platform demonstrates excellent ΔΨm-driven mitochondrial targeting, real-time fluorescence imaging ability (highly overlapping), and significant antitumor selectivity (cell-specific cytotoxicity with a >15-fold selectivity). Mechanistic investigations revealed that PBD exerts its antitumor effects through mitochondrial membrane potential collapse and subsequent superoxide burst, ultimately triggering apoptosis. This multimodal strategy establishes a new paradigm for image-guided precision therapy in mitochondrial dysfunction-related malignancies.
研究领域:
药物化学
·
肿瘤学
·
生物化学
·
药理学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2021
·
IF 5.537
Ranran Xu,Limin Wang,Kexin Li,Jiankang Cao,Zhilei Zhao
Plum fruit often suffer severe chilling injury (CI) during storage. Plum ( Prunus salicina Lindl. cv. Friar) fruit were treated with melatonin at various concentrations and stored at 0 ℃ for 6 w and additional 3 d of shelf-life at 23 ℃. Observations revealed that the treatment with 1.0 mmol L −1 melatonin mitigated CI of the fruit by reducing flesh-reddening and ‘ethylene burst’. The melatonin treatment also suppressed fruit softening and maintained energy status. Results from UPLC-MS assay showed that several individual phenolic compounds involved in the biosynthesis of flavonoids and anthocyanins, especially, the red pigment of cyanidin-3- O -glucoside, were stimulated by cold stress and its removal, but the stimulation was effectively inhibited by melatonin treatment, and thereby flesh-reddening was alleviated. Results from RNA-sequencing unraveled that cold stress and its removal altered transcriptomic profiling of the plum fruit, but the melatonin treatment hindered the transcription of genes related to the anthocyanin biosynthesis. Correlation analysis indicated that seven MYBs were positively correlated with anthocyanin biosynthesis, which were generally down-regulated in the melatonin-treated plums. The integrative analysis of transcriptomic and metabolic alterations revealed differentially but delicately coordinated inhibition of postharvest physiological metabolisms of ‘Friar’ plums by melatonin in response to cold stress.
研究领域:
园艺学
·
植物生理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Wensheng Lin,Shixian Lin,Xingwu Zhou,Fanwen Yang,Zishan Lin,Shiqing Li,Haoyuan Zhang,Yuehan Ouyang,Jieying Zhu,Wei Sun,Dequn Huang,Baojian Liao,Jixiang Zhu
As a minimally invasive drug delivery platform, microneedles (MNs) overcome many drawbacks of the conventional transdermal drug delivery systems, therefore are favorable in biomedical applications. Microneedles with a combined burst and sustained release profile and maintained therapeutic molecular bioactivity could further broaden its applications as therapeutics. Here, we developed a double-network microneedles (DN MNs) based on gelatin methacrylate and acellular neural matrix (GelMA-ACNM). ACNM could function as an early drug release matrix, whereas the addition of GelMA facilitates sustained drug release. In particular, the double-network microneedles comprising GelMA-ACNM hydrogel has distinctive biological features in maintaining drug activity to meet the needs of application in treating different diseases. In this study, we prepared the double-network microneedles and evaluated its morphology, mechanical properties, drug release properties and biocompatibility, which shows great potential for delivery of therapeutic molecules that needs different release profiles in transdermal treatment.
研究领域:
药剂学
·
生物材料
·
药物递送系统
·
透皮治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yalan Wu,Nan Zhao,Yiwei Jiang,Xiaodong Zheng,Ting Yu,Fujie Yan
Yeast-derived cell wall polysaccharides possess numerous biological activities, but their application in postharvest preservation is rarely reported. The aim of this research was to investigate the effects of Kluyveromyces marxianus soluble cell wall polysaccharide (SCWP) on preventing the infection of Penicillium expansum in pear fruit. The results showed that K. marxianus SCWP treatment could significantly improve the resistance of pear fruit to P. expansum , with respect to Saccharomyces cerevisiae -derived SCWP. Composition of both SCWPs was mannan with the main chains consisting of a → 6)-α-D-Manp-(1 → unit and the branch structure formed by → 2)-α-D-Manp-(1 except that K. marxianus SCWP took on a shorter side chain and a rougher surface than S. cerevisiae SCWP. In addition, mechanisms of K. marxianus SCWP on stimulating resistance response were associated with the apparent oxidative burst, increased gene expression and enzyme activity of antioxidant and defense systems in pear fruit. Our findings suggest that K. marxianus SCWP can be used as an innovative and promising candidate for preventing postharvest fungal decay and extending fruit shelf life.
研究领域:
农业科学
·
植物病理学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）