Enzyme mimics have been developed as a new generation of antibiotics with broad-spectrum antibacterial properties to combat bacteria. In this study, a peroxidase mimic was constructed from nanodiamonds (NDs) to treat periodontal pathogen-induced infections with decreasing damage of periodontal tissues by traditional drug therapy. The oxygenated nanodiamonds (O-NDs) have excellent enzymatic activity over a broad pH range, and locally activated free-radical generations from low concentration of hydrogen peroxide, presenting a significantly enhancement in bacterial cell membrane disruptions and breaking down biofilms. In the presence of trace non-toxic hydrogen peroxide, topical rinse treatments with O-NDs restrain the development of bacterial infection in vivo , thus preventing and ameliorating the course of periodontal inflammation in a rodent model. More importantly, the enzymatic activity of O-NDs can accelerate the wound healing around sites of periodontal infection in time. The platform of nanodiamond-based enzyme mimic is built having high performance antibiotic-free periodontal antibacterial therapy, and can be extended to other fields of medical applications.
Fan Dong,Yunhang Liu,Huihua Dong,Xing Sun,Bolong Xu,Qingyuan Wu,Xican Xu,Yong Jiang,Huiyu Liu,Yuguang Wang
Using nanomaterials with photodynamic therapy (PDT) to combat bacterial infections including periodontal infections has attracted the attention of researchers because of their high efficiency, simplicity and low susceptibility to drug resistance. However, the therapeutic effectiveness is threatened by the endogenous accumulation of hydrogen peroxide and insufficient amounts of the substrate oxygen. Here, we report a microporous Zr porphyrin carboxylate metal-organic framework (MOF) PCN-222 doped with platinum nanozymes, Pt@PCN-222, to reshape the microenvironment that is not conducive to the recovery of infected tissue and further promote recovery after periodontal tissue infection. This MOFdoped with platinum nanozymes can remove excessive endogenous hydrogen peroxide and produce dissolved oxygen simultaneously. This framework can effectively alleviate anaerobic bacterial infection in periodontal tissue, including efficiently killing the bacteria and reducing the level of inflammation in tissue. This work provides a new strategy to solve the long-term clinical challenge of anaerobic bacterial infections in periodontal tissue and proposes an innovative approach to improve treatment.
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