CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Yuerong Yang,Yu Lu,Yinchuan Liu,Yu Pan,Hongjuan Ma,Linjuan Huang,Zhongfu Wang
Yak milk (YM) has higher protein content than other bovine milk (BM) varieties. The bioactivity of milk glycoproteins is related to N / O -glycans. We qualitatively and quantitatively compared the N / O -glycome of YM and BM glycoproteins using stable isotope labeling combined with hydrophilic interaction chromatography and electrospray ionization mass spectrometry. We identified 79 and 78 N -glycans in YM and BM, respectively. Two N -glycans (H4N5F1A1; H5N4F1) were exclusive to YM. The content ratios of different types of N -glycans differed significantly between YM and BM, with sialylated N -glycans 2.33 times more abundant in YM. Five and seven O -glycans were detected in YM and BM, respectively. Two O -glycans (H1N2; H1N2A1) were exclusive to BM. The bi-sialylated O -glycan, H1N1A2, accounted for 56.1% of O -glycans in YM; it was 5.97 times more abundant in YM than in BM (equal volume basis). This study provides a theoretical basis for the future utilization of YM as a functional food.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jinjin Tong,Zezheng Liu,Kexuan Zhou,Kaiming Wang,Shijiao Guo,Hua Zhang
The overuse of antibiotics in livestock production poses a significant threat to public health, primarily by accelerating the emergence of antibiotic-resistant bacteria—a critical global issue. Therefore, the development of effective alternatives to antibiotics is urgently required to address this problem. In this study, BLfcin-NPs@CS/β-GP thermosensitive hydrogels were developed by incorporating bovine lactoferricin-loaded chitosan nanoparticles into a chitosan/β-GP hydrogel matrix. Scanning electron microscopy (SEM) revealed the formation of a three-dimensional porous structure. Fourier-transform infrared (FTIR) spectroscopy confirmed successful synthesis, while X-ray diffraction (XRD) analysis indicated that nanoparticle incorporation did not alter the crystallinity of the hydrogel. The rheological properties, in vitro degradation, biocompatibility, and antibacterial activity of the composite hydrogels were systematically evaluated, and the results demonstrated that the composite hydrogels exhibited temperature sensitivity, enhanced degradation rates, low cytotoxicity, and strong antibacterial activity. These findings suggest that BLfcin-NPs@CS/β-GP hydrogels present a promising alternative to antibiotics for treating bacterial infections, such as bovine mastitis, offering an innovative and effective strategy to reduce antibiotic use and mitigate the risk of antibiotic resistance in livestock production.
研究领域:
兽医学
·
生物材料学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
RESEARCH IN VETERINARY SCIENCE
·
2023
·
IF 2.4
Jirao Shen,Feng Yang,Guibo Wang,Xiaoqing Mou,Jinyu Li,Xuezhi Ding,Xurong Wang,Hongsheng Li
Staphylococcus haemolyticus ( S. haemolyticus ) is one of the most common coagulase-negative staphylococci (CoNS) isolates from bovine mastitis . Paeoniflorin (PF) shows anti-inflammatory effects on different inflammatory diseases in vitro studies and in vivo animal experiments. In this study, the viability of bovine mammary epithelial cells (bMECs) was detected by the cell counting kit-8 experiment. Subsequently, bMECs were induced with S. haemolyticus , and the induction dosage was determined. The expression of pro-inflammatory cytokines and toll-like receptor (TLR2) and nuclear factor kappa-B (NF-κB) signaling pathway-related genes were investigated by quantitative real-time PCR. The critical pathway proteins were detected by western blot . The results showed that the multiplicity of infection (MOI; the ratio of bacteria to bMECs) 5:1 of S. haemolyticus for 12 h could cause cellular inflammation, which was selected to establish the inflammatory model. Incubation with 50 μg/ml PF for 12 h was the best intervention condition for cells stimulated by S. hemolyticus . Quantitative real-time PCR and western blot analysis showed that PF inhibited the activation of TLR2 and NF-κB pathway-related genes and the expression of related proteins. Western blot results showed that PF suppressed the expression of NF-κB unit p65, NF-κB unit p50, and MyD88 in bMECs stimulated by S. haemolyticus . The inflammatory response pathway and molecular mechanism caused by S. haemolyticus on bMECs are related to TLR2-mediated NF-κB signaling pathways. The anti-inflammatory mechanism of PF may also be through this pathway. Therefore, PF is expected to develop potential drugs against CoNS-induced bovine mastitis.
研究领域:
兽医学
·
免疫学
·
药理学
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Zhenhua Li,Xiaoqin Wang,Xiaoli Deng,Jiansen Song,Tong Yang,Yujie Liao,Guiping Gong,Linjuan Huang,Yu Lu,Zhongfu Wang
Glycosphingolipids (GSLs) in human milk regulate the immune system, support intestinal maturation, and prevent gut pathogens. The structural complexity and low abundance of GSLs limits their systematic analysis. Here, we coupled the use of monosialoganglioside 1–2-amino-N-(2-aminoethyl) benzamide (GM1-AEAB) derivatives as internal standards with HILIC-MS/MS to qualitatively and quantitatively compare GSLs in human, bovine, and goat milk. One neutral glycosphingolipid (GB) and 33 gangliosides were found in human milk, of which 22 were newly detected and three were fucosylated. Five GB and 26 gangliosides were identified in bovine milk , of which 21 were newly discovered. Four GB and 33 gangliosides were detected in goat milk, 23 of them newly reported. GM1 was the main GSL in human milk; whereas disialoganglioside 3 (GD3) and monosialogangloside 3 (GM3) were dominant in bovine and goat milk, respectively; N -acetylneuraminic acid (Neu5Ac) was detected in >88 % of GSLs in bovine and goat milk. N -hydroxyacetylneuraminic acid (Neu5Gc)-modified GSLs were 3.5 times more abundant in goat than in bovine milk; whereas GSLs modified with both Neu5Ac and Neu5Gc were 3 times more abundant in bovine than in goat milk. Given the health benefits of different GSLs, these results will facilitate the development of custom-designed human milk-based infant formula.
研究领域:
营养学
·
生物化学
·
代谢组学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Yue Jia,Yu Lu,Xue Wang,Yuerong Yang,Meiyi Zou,Jie Liu,Wanjun Jin,Xiaoqin Wang,Guoliang Pang,Linjuan Huang,Zhongfu Wang
Bovine lactoferrin is a functional N-glycoprotein whose glycan moieties play an important role in its biological activities. Although bovine lactoferrin is rich in sialylated N -glycans, there are no reports on the differentiation between α2,3-linked and α2,6-linked sialylated N- glycan isomers. In this study, bovine lactoferrin was separated and purified by cation exchange chromatography, and >95% pure protein was obtained. Following this, N- glycan derivatives of lactoferrin at different stages of lactation were qualitatively and quantitatively analyzed by MALDI-TOF-MS and HILIC-MS/MS. With prolonged lactation, the relative content of the sialylated and fucosylated bi-antenna complex N -glycan (Hex1Man3GlcNAc4GalNAc1Fuc1Neu5Ac1) changed the maximum, with 14.5%, 1.3%, and 0.9% contents in transitional milk, colostrum, and mature milk, respectively. Meanwhile, the contents of sialylated and fucosylated N -glycans in transitional milk were the hi ghest . More importantly, the sialylated N- glycans-linked isomers could be distinguished by linkage-specific derivatization of sialic acid . There were 11 α2,3- and 13 α2,6-linked sialyation N- glycans isomers in the colostrum, which transformed to 4 and 3 N- glycan isomers, respectively, in the mature milk. These findings provide a foundation for an in-depth research on the structure–function relationship of bovine lactoferrin.
研究领域:
生物化学
·
营养学
·
分子生物学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2025
·
IF 3.7
Jing-Jing Sun,Ruo-Que Mao,Xiao-Qian Fu,Shan-Jing Yao,Dong-Qiang Lin
The exosomes hold significant potential in disease diagnosis and therapeutic interventions. The objective of this study was to investigate the potential of aqueous two-phase systems (ATPSs) for the separation of bovine milk exosomes. The milk exosome partition behaviors and bovine milk separation were investigated, and the ATPSs and bovine milk whey addition was optimized. The optimal separation conditions were identified as 16% (mass) polyethylene glycol 4000, 10% (mass) dipotassium phosphate, and 1% (mass) enzymatic hydrolysis bovine milk whey. During the separation process, bovine milk exosomes were predominantly enriched in the interphase, while protein impurities were primarily found in the bottom phase. The process yielded bovine milk exosomes of 2.0×10 11 particles·ml −1 whey with high purity (staining rate>90%, 7.01×10 10 particles·mg −1 protein) and high uniformity (polydispersity index <0.03). The isolated exosomes were characterized and identified by transmission electron microscopy, zeta potential and size distribution. The results demonstrated aqueous two-phase extraction possesses a robust capability for the enrichment and separation of exosomes directly from bovine milk whey, presenting a novel approach for the large-scale isolation of exosomes.
研究领域:
生物技术
·
医学工程
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 3
·472
·
IF 5.561
Mengjie Zhang,Xiaomeng Sun,Jianjun Cheng,Mingruo Guo
Pet foods are gaining ground in China. Canine milk substitute formulations are based on their milk chemistry. This study aimed to analyze and compare the differences in proteins, fatty acids, minerals, and basic chemical composition between canine, bovine, and caprine milk. Canine milk contains higher contents of protein (6.62–17.34%), fat (8.92–14.31%), and ash (1.11–1.81%), and a lower content of lactose (1.56–3.92%) compared to bovine and caprine milk. The protein profiles of canine, bovine, and caprine milk were similar as confirmed by sodium dodecyl sulphate-polyacrylamide electrophoresis gel (SDS-PAGE). The quantification of proteins in canine, bovine, and caprine milk were significantly different when analyzed by inverse high-performance liquid chromatography. Canine milk showed higher contents of monounsaturated fatty acids (29.71–32.95% of total fatty acids) and polyunsaturated fatty acids (16.83–20.56% of total fatty acids), but a lower proportion of saturated fatty acids (47.57–53.18% of total fatty acids) than bovine and caprine milk. The essential fatty acids ARA and DHA were also found in canine milk in the ranges of 0.82–1.77% and 0.12–0.43% of total fatty acids, respectively. Canine milk had higher levels of Mg, K, Na, Fe, and Zn than those in bovine and caprine milk. The nutrient profile of canine milk was different from that of bovine and caprine milk. There were differences in nutritional compositions of the milk samples from four breeds, and Rottweiler milk had the highest nutritional content. The data of this study may provide useful information about the nutritional needs of puppies during their first months of life and the basic information for formulations of puppy milk substitutes.Keywords:canine milk;chemical composition;protein;fatty acids;mineral
研究领域:
动物科学
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营养学
·
兽医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Jing-Yan Li,Qian Ge,Bin Liu,Zhen-Peng Gao,Chun-Feng Guo
This study aimed to develop a non-protein-based method for quantify cow milk in adulterated goat milk. The raw cow milk and raw goat milk samples were mixed separately with the same volume of cold acetone and centrifuged to remove their proteins. The supernatants were evaporated, and the residues were derivatized by silylation followed by GC-MS analysis. N-acetylglucosamine was identified as a thermostable non-protein biomarker of cow milk. Its concentration in the raw cow milk (146.7 mg/L) was 32 times higher than that in the raw goat milk. On this basis, a HPLC-MS/MS method using positive-ion electrospray ionization in parallel reaction monitoring mode was developed for quantifying cow milk in adulterated goat milk by determining peak area of the biomarker. The method showed good linearity over cow milk concentrations of 1–100% and could detect as low as 0.3% cow milk in adulterated goat milk. Besides, the method was characterized by a simple sample pretreatment process, rapid detection, high accuracy, and high precision. With this method, a sample could be analyzed in 10 min. The present study demonstrated the feasibility of quantifying cow milk in adulterated goat milk using N-acetylglucosamine as a biomarker of cow milk.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Shaofeng Yuan,Changjian Li,Yichi Zhang,Hang Yu,Yunfei Xie,Yahui Guo,Weirong Yao
The removal of pesticide residues in food by ultrasound has attracted more attention in recent years, and the formation of intermediate products may have some profound effects on the toxicity of treated food. Therefore, degradation of parathion methyl (PM) in bovine milk by ultrasonic treatment was studied in this paper. Results showed that the ultrasonic intensity and the initial concentration of PM had a significant effect on the degradation rate of PM (P < 0.05). The maximum degradation rate of PM was 97.10%. Three transformation products were identified through UPLC-QTOF/MS analysis, and the oxidation pathway was proposed as the consequence of ultrasonication. Furthermore, according to Quantitative Structure Activity Relationship (QSAR) model prediction, the ecotoxicity of the transformation products may be higher than that of PM. These findings showed that although ultrasonic treatment can effectively degrade pesticide residues in food, it may also generate transformation products with the higher ecotoxicity.
研究领域:
食品科学
·
环境毒理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2019
·Vol. 43
·期 2
·398-405
·
IF 2.516
Xiaojing Lai,Jing Liu,Xin Xu,Jie Li,Baojun Zhang,Lijun Wei,Hongping Cai,Xianglei Cheng
In reversed-phase liquid chromatography, tetracycline antibiotics yield broad and asymmetrical peaks, as a result of their ionic interaction with the anionic free silanol groups and metal ion present in the silica-based stationary phases (commonly derivatized with C18 groups). These frequently encountered difficulties were absolutely eliminated based on dual effect when methanesulfonic acid was employed as an eluent additive. The study revealed that the performance of methanesulfonic acid to minimize the “silanol effect” is mainly explained by both direct neutralization of the anionic silanol sites and electrostatic attraction with analytes. Based on these dual action mechanisms, an ultrasensitive method has been successfully developed for the simultaneous determination of tetracycline antibiotics and their derivatives (minocycline, oxytetracycline, tetracycline, chlortetracycline, metacycline, doxycycline, 4-epitetracycline, and 4-epichlortetracycline) in bovine milk with convenient ultraviolet detection within 15 min. Under the optimal conditions, the calibration curves showed good linearity ( r 2 > 0.999) for all analytes in the range of 1∼200 ng/mL with the instrument limits of detection as low as 0.3 ng/mL. The study sheds new light on suitable additives to analyze basic compounds with the advantage of good compatibility with MS detection.
研究领域:
分析化学
·
药物分析
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL DAIRY JOURNAL
·
2021
·
IF 3.032
Lin Yang,Yan Zhao,Fang Wang,Jie Luo
The regiodistribution of fatty acids (FAs) in triacylglycerols plays a critical role in the digestion and adsorption of milk lipids . However, the stereospecific location of FAs in yak milk triacylglycerols remains unknown. In this study, the intrapositional and interpositional distribution of FAs in the yak milk from different pastoral areas were compared with those of human milk and cow milk. When compared with human milk, the stereospecific locations of FAs were relatively similar between yak milk and cow milk. However, White yak milk and human milk contained similar interpositional proportions of linoleic, α -linolenic acid and polyunsaturated FAs at the sn -3 position, significantly higher than those of cow milk. Moreover, the sn -3 interpositional proportion of α-linolenic acid in Maiwa yak milk was even higher than that in human milk. The study suggests that yak milk might provide better nutritional benefits as a source of essential FAs than cow milk.
研究领域:
食品科学
·
动物学
·
营养学
✓ 已验证
DOI
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Longyu Wan,Wen Tu,Jiaxin Zhang,Jiayue Yang,Xu Wang,Jian He,Chaoxin Man,Qianyu Zhao,Feng Zhao,Yujun Jiang
Goat's milk-based infant formula (YIF) has the advantages of high nutritional value and hypoallergenicity, and fewer studies have been conducted on YIF. Therefore, this study examined the changes in physicochemical and functional properties of cow's milk-based infant formula (ZIF) and YIF during 6 months of storage at 25 °C, 37 °C, and 50 °C, respectively. The results showed that YIF had higher pH (7.26), wettability, protein oxidation, good wettability, and lower lactose crystallinity and lipid oxidation compared to ZIF. Further analysis revealed that the higher pH significantly accelerated the rate of the Maillard Reaction (MR), resulting in significantly higher browning of YIF than ZIF, and this difference was statistically significant ( p < 0.05). This is one of the reasons for the decrease in solubility of YIF. This paper explores the differences in storage stability of ZIF and YIF and provides theoretical guidance for the selection of milk-based ingredients.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）