JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
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IF 4
Bin Li,Wenjing Gao,Ling Ling,Shaoning Yu,Liangqiang Chen,Fan Yang
Lactose is an important nutrient but its content in milk products poses concerns for people with lactose intolerance . Thus, a simple, rapid, and high-throughput assay for lactose determination is needed to maintain specified lactose levels during production and in final milk products. Here, we report a reactive matrix-assisted laser desorption/ionization mass spectrometry ( Re MALDI MS) method for the determination of lactose in milk products. The selective reactivity and the excellent laser absorption properties of 4-hydrazinoquinazoline (4-HQ) matrix increase the sensitivity of traditional MALDI MS analysis of lactose by 100 folds. After optimization of the method, lactose was detected distinctly in milk without pretreatment . Using xylobiose as an internal standard, the assay accurately quantified lactose in the concentration range of 1.00−700 μg/mL (R 2 = 0.994). Due to the high sensitivity and specificity, the lactose content in different types of milk samples was determined successfully, as well as the residual lactose in low-lactose and lactose-free milk.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
Foods
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2024
·Vol. 13
·期 13
·2143
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IF 4.7
Shuangshuang Cui,Yong Wei,Can Li,Jian Zhang,Yunfeng Zhao,Xiayu Peng,Fengxia Sun
(1) Background: Rapid on-site testing is an effective method for the detection ofEscherichia coli O157: H7(E. coli O157: H7) in food ingredients and the environment. (2) Methods: In this study, we developed colorimetric loop-mediated isothermal amplification (LAMP) and immunochromatographic test strips (ICTs) for the rapid and visual detection ofE. coli O157: H7. This study designed new specific LAMP primers forE. coli O157: H7virulence island genes. After the LAMP amplification, the double-stranded DNA target sequence labeled with digoxin and fluorescein isothiocyanate (FITC) at both ends was bound to the anti-digoxin antibody on the gold nanoparticles. Subsequently, it was further bound to the anti-FITC antibody at the T line of the ICTs, forming a positive test result. Hydroxynaphthyl blue dye was directly added to the LAMP amplification product. A blue color indicated positive results, while a purple color indicated negative results. (3) Results: Two visualization methods showed high specificity for the target strains. The visualization tests had sensitivities of 5.7 CFU mL−1, and the detection limit of theEscherichia coli O157: H7in artificially contaminated milk samples was 5.7 × 102CFU mL−1, which was consistent with the results of the standard method (LAMP-electrophoresis method) used in commercial inspection. (4) Conclusions: Both methods could be useful in remote and under-resourced areas.
研究领域:
食品安全
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分子生物学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Juanhua Zhang,Yuzhen Li,Mengxiao Lv,Yihui Bai,Zhenmin Liu,Xuexiang Weng,Chunping You
5-Hydroxymethylfurfural (5-HMF) is a useful indicator of thermal damage degree and freshness of milk. It is of great importance to develop simple, rapid and accurate analytical methods for the sensitive detection of 5-HMF in milk and milk-based products. In this work, surface-enhance Raman spectroscopy (SERS) was used for rapid determination of 5-HMF in processed cheese by colloidal Au nanoparticles (AuNPs) substrate synthesized by the classical solvothermal reduction method. Density functional theory (DFT) calculations were carried out to determine the vibration assignments of 5-HMF and the surface enhancement effect of AuNPs substrate. The results found that a good linear response on the AuNPs substrate for 5-HMF in the concentration range of 0.1–75 mM was established with the detection limit of 75 μM (S/N = 3). Furthermore, the present method could be applied to the determination of 5-HMF in a cheese real sample which revealed its promising application in food safety and analysis.
研究领域:
食品安全
·
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
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IF 3.858
Yanan Yuan,Mingwei Wang,Nan Jia,Chengcheng Zhai,Yehong Han,Hongyuan Yan
The illegal addition and abuse of 17β-estradiol (E2) in food has led to its accumulation in the body through the food chain, resulting in adverse effects on the human body through interference with the endocrine system. Because milk and dairy products are major components of the human diet, screening as well as quantitative analysis of E2 in these food types is important. In this work, a three-dimensional graphene/multi-walled carbon nanotube composite was synthesized and applied to a home-made pipette-tip solid-phase extraction cartridge and coupled with high-performance liquid chromatography-fluorescence detection to determine E2 in milk. The established method showed good linearity in the range of 5–250 ng mL −1 with correlation coefficient of 0.9998. The detection limit and quantitation limit were 0.7 and 2.3 ng mL −1 , respectively. The repeatability and intermediate precision, expressed as the coefficient of variation were 3.7% and 6.0%, respectively. The accuracy of the method was investigated by recovery experiments with milk at three spiked levels (25, 100, and 250 ng mL −1 ), where the recoveries were in the 88.4–108.9% range with relative standard deviations ≤5.5%. The proposed method has the advantage of requiring less adsorbent (only 1.0 mg) and less organic reagent (1.0 mL of washing solvent, 1.2 mL of elution solvent) and exhibits excellent purification ability, with high sensitivity and accuracy, and was successfully applied for the determination of E2 in milk products.
研究领域:
分析化学
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食品安全
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2020
·
IF 4.049
Xiaowei Sun,Wenhua Ji,Shenghuai Hou,Xiao Wang
Trifluoromethyl covalent organic framework (CF 3 -COF) is facilely synthesized at room temperature using for solid-phase microextraction (SPME). The CF 3 -COF coating displays an improved extraction performance for per- and polyfluorinated alkyl substances (PFASs) comparing to amorphous polymer coating, non-contained fluorine covalent organic framework coating and commercially available SPME coating. The fluorine affinity of trifluoromethyl in CF 3 -COF for PFASs is demonstrated using the density functional theory calculation. Following the microextraction with CF 3 -COF-coated fiber, the targeted PFASs are eluted with 1 mL acetonitrile and quantified by ultrahigh performance liquid chromatography tandem mass spectrometry. The low limits of detection are 0.1–0.7 pg g −1 for milk and 0.2–0.8 pg g −1 for milk powder, suggesting the outstanding sensitivity. The precision for the method ranges between 2.9 and 9.9%. The fiber-to-fiber repeatability is in the range of 4.2–9.5%. Under the optimum conditions, the relative recoveries are 91.0–110% with RSDs ≤ 10% for milk and 89.8–111% with RSDs ≤ 10% for milk powder.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）