VIBRATIONAL SPECTROSCOPY
·
2022
·
IF 2.382
Xiong Chen,HongGao Liu,JieQing Li,YuanZhong Wang
Wild boletes, as a nutritious food extensively used in food fields. It is rich in polyphenolic compounds, which is it can promote human health, improve physical performance and reduce the risk of developing diseases. But the existing methods could not evaluate the total polyphenol content (TPC) quickly and accurately. In this study, two-dimensional correlation spectroscopy (2D-COS) images were generated by high performance liquid chromatography (HPLC) and Fourier transform near-infrared (FT-NIR) spectroscopy using a generalized two-dimensional correlation algorithm. In addition, the TPC of all samples was determined. Residual convolutional neural network (ResNet) models were then established to identify different levels of TPC. The results shown that compared with HPLC, FT-NIR combined with 2D-COS algorithm had excellent identification performance and greatly reduces the time of data processing. The accuracy rates of training sets and testing sets were 100%. The established method can be effectively applied to Lanmaoa asiatica , Butyriboletus roseoflavus and Rugiboletus extremiorientalis . This work provides a novel and comprehensive strategy for other nutritious foods.
研究领域:
食品科学
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分析化学
·
机器学习
·
真菌学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 13
·1839
·
IF 5.561
Qiao Yang,Xiaoyi Zhang,Huini Qin,Feijun Luo,Jiali Ren
Cancer is still the leading cause of death across the world, and there is a lack of efficient therapies.Lactarius hatsudakeis a mushroom with a food and medicine homology that contains numerous biologically active substances. This study aimed to investigate the composition of extracts fromLactarius hatsudake(L. hatsudake) and their anti-cancer function and molecular mechanisms. Our results showed that the total phenolic content ofL. hatsudakeextracts was 139.46 ± 5.42 mg/g. The following six phenolic compounds were identified fromL. hatsudakeextracts by HPLC and UPLC-QTOF/MS: gallic acid, pyrogallol, chlorogenic acid, ferulic acid, myricetin, and cinnamic acid. Colorectal cancer cell HCT116 and hepatic cancer cell HepG2 were used to evaluate the anti-cancer function of theL. hatsudakeextracts. Compared with HepG2 cells, theL. hatsudakeextracts showed stronger anti-cancer activity against HCT116 cells and these were used to study molecular mechanisms. The results indicated that theL. hatsudakeextracts could arrest the cancer cell cycle and inhibit cancer cell proliferation, which may be mediated by the MAPK/NFκB/AP-1 signalling pathway; theL. hatsudakeextracts also promoted cancer cell apoptosis through a mitochondrial-dependent pathway. Taken together, these findings demonstrate thatL. hatsudakeethanol extracts contain six main phenolics and illustrate the remarkable potentiality ofL. hatsudakeas a source of natural phenolics for cancer prevention and as an adjuvant in the treatment of functional foods.Keywords:Lactarius hatsudake;phenolic acids;anti-cancer;molecular mechanism
研究领域:
药用真菌学
·
肿瘤生物学
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植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2020
·Vol. 55
·期 6
·2492-2508
·
IF 2.773
Yuanyuan Zhao,Xuefeng Chen,Wei Jia,Guoli Gong,Yanni Zhao,Guoliang Li,Jie Zhou,Xiaona Li,Yu Zhao,Wenjin Ma
Summary Pleurotus eryngii polysaccharide (PEP) was obtained using hot water extraction and ethanol precipitation. Three purified polysaccharide fractions (namely PEP1-A, PEP2-A and PEP3-A) were obtained from PEP using DEAE-cellulose-52 chromatography and a gel permeation Sephadex G-100 column. Firstly, this paper examined the characterisation of PEP1-A, PEP2-A and PEP3-A. The corresponding molecular weights were 5.378 × 10 5 , 9.506 × 10 6 and 4.975 × 10 5 Da, respectively. PEP1-A, PEP2-A and PEP3-A had similar monosaccharide compositions. PEP1-A was β-configuration, and PEP2-A and PEP3-A were α-configuration. PEP1-A, PEP2-A and PEP3-A had pyran-type rings, (1 → 3) glucose and (1 → 6) galactose linkages. Secondly, PEP, PEP1-A, PEP2-A and PEP3-A possessed antioxidant activities, and PEP was best. Therefore, only PEP was used to study its anti-fatigue activity in vivo . The result proved that PEP had anti-fatigue activity. PEP could be used as a valuable natural food supplement for preventing anti-fatigue or functional food.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
NANO
·
2017
·
IF 1.025
Guofu Ma,Wei Tang,Kanjun Sun,Zhiguo Zhang,Enke Feng,Ziqiang Lei
Coprinus comatus-based nitrogen-doped activated carbon (N-ACC) is prepared by chemical activation and nitrogen-doped methods. The N-ACC possesses large specific surface areas (976.96 m 2 g − 1 ), high nitrogen contents (11.53 wt.%), and super hydrophilicity. As electrode material for supercapacitors, the N-ACC shows remarkable electrochemical performance, such as 346 F g − 1 maximum specific capacitance at a current density of 1 A g − 1 , which retains 260 F g − 1 even at a high current density of 10 A g − 1 (about 75% capacitance retention) in 2 M KOH aqueous electrolyte. The assembled N-ACC//N-ACC symmetric supercapacitor exhibits energy density of 14.63 Wh kg − 1 at power density of 810 W kg − 1 , and excellent cycling stability with 92% specific capacitance retention after 10000 cycles in the voltage range 0–1.8 V in 0.5 M Na 2 SO 4 aqueous solution. These results indicate that the N-ACC as electrode materials can be used for high performance supercapacitors.
研究领域:
材料科学
·
能源技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2020
·
IF 4.972
Huiying Zhang,Dian Huang,Dandan Pu,Yuyu Zhang,Haitao Chen,Baoguo Sun,Fazheng Ren
The present research investigated the relationships among sensory attributes and volatile components in dry porcini mushrooms by multivariate statistical analyses. The sensory characteristics were based on quantitative descriptive analysis and consumer hedonic assessment. The volatile compounds were extracted by solid-phase microextraction, then were identified using gas chromatography-mass spectrometry and gas chromatography-olfactometry. The results showed that the high hedonic rating of porcini mushrooms was due in part to its aroma notes such as seasoning-like, roasted, cacao-like and smoky. High hedonic liking and positive aromas of porcini mushrooms could be responsible for the volatiles including 3-(methylthio)propanal, 3-(methylthio)propanol, pyrazines, phenolic and furanone components. On the other hand, raw mushroom-like, cardboard-like, as well as sweaty attributes were attributed to the relatively low hedonic liking of porcini mushrooms, which correlated with high contents of 1-octen-3-ol, octanal, 2-pentylfuran and 3-methylbutanoic acid. The information reported here could be important for the quality control of commercial porcini mushrooms by providing an approach to strengthen the interpretation of sensory data by showing how they were affected by the chemical properties.
研究领域:
食品科学
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化学分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2022
·Vol. 37
·期 9
·1547-1556
·
IF 2.613
Like Zhou,Hui Zhou,Huilong Xiao,Zihang Zhang,Ziyun Xiong,Xun Tuo,Hui Guo
Nifedipine (NDP), a dihydropyridine calcium antagonist, is widely used for the treatment of hypertension and angina pectoris. Catalase is a key antioxidant enzyme that is closely relevant to the level of reactive oxygen specie in vivo . Here, the research explored the effects of NDP on the conformation and catalytic function of bovine liver catalase (BLC) through enzymatic reaction kinetic techniques, multispectroscopic analysis, and computer simulation methods. Kinetic studies clarified that the NDP reduced the activity of BLC using a noncompetitive inhibition mechanism. Based on trial data, a static quenching mechanism functioned in quenching the intrinsic fluorescence of BLC. The binding constant value was (4.486 ± 0.008) × 10 4 M −1 (298 K) and BLC had one binding site for NDP. Tyr was prone to be exposed more to a hydrophilic environment in wake of a shift in fluorescence value. The binding reaction of BLC to NDP caused a conformational change in BLC, which in turn led to increase in the α-helix content and a decline in the β-sheet content. Furthermore, several amino acids residues interacted with NDP by means of van der Waals forces, whereas Gln397, Asn368, Gln371, Asn384, and Pro377 formed several hydrogen bonds with NDP.
研究领域:
生物化学
·
药物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 12
·2908-2919
·
IF 2.413
Xie Zenghui,Zhang Lanyun,Chen Yanli,Hu Xujia
In this study, novel magnetic molecularly imprinted polymers (MMIPs) with specific adsorption for glutathione were prepared. Based on MMIPs as adsorbents for magnetic solid-phase extraction, we combined magnetic solid-phase extraction with high-performance liquid chromatography (MPSE-HPLC) for the separation, enrichment, and detection of glutathione in nine kinds of wild edible bolete extracts. MMIPs were characterized by Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and vibrating sample magnetometer (VSM). In addition, the properties of MMIPs were studied by adsorption isotherm, adsorption kinetic, and selective adsorption experiments. The results showed that MMIPs had high selectivity for glutathione ( K d = 245.33 mL/g), and adsorption time and saturated adsorption capacity were 30 min and 17.88 μmol/g, respectively. Under the optimized conditions, there was a good linear relationship in the concentration range of 100~3000 μg/L, the correlation coefficient R 2 = 0.9997, and the limit of detection (LOD) and the limit of quantitation (LOQ) that were 24.828 μg/L and 82.760 μg/L, respectively. The spiked recovery and RSD were 93.28~103.03% and 2.77~5.11, respectively. We successfully combined MSPE-HPLC for the detection of glutathione in nine kinds of wild edible bolete extracts. It was found that the content of glutathione in Leccinum extremiorientale (L. Vass.) Sing. was the highest (19.800 mg/g) and that in Boletus aereus was the lowest (6.791 mg/g).
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Rui Mao,Wei Li,Penghao Jia,Hui Ding,Tekleab Teka,Lin Zhang,Zhifei Fu,Xuebin Fu,Sunjay Kaushal,Zhiying Dou,Lifeng Han
Unsaturated fatty acids (UFAs) are fatty acids with one or more carbon-carbon double bonds (C=C). They are building blocks of structurally complex lipids. According to the position of C=C bond in the UFAs, there are multiple isomers with different physiological activities. Therefore, an accurate understanding of UFAs is essential. Combining Paternò-Büchi (PB) reaction with tandem mass spectrometry (MS/MS) analysis has been successfully used to identify unsaturated lipids in bio-samples. However, there are still some challenges, such as low reactive rate and low MS response of PB products. In this study, 3-pyridinecarboxaldehyde (3-PYA) was selected as an efficient PB reagent for the first time. According to the nitrogen rule, introduction of nitrogen did not only make the target MS and MS/MS ions to be easily identified but also increase the ionization efficiency significantly. The developed method in this study demonstrated greater efficiency and sensitivity for the identification of UFAs in total lipid extracts from bovine liver compared with other commonly used PB reagents.
研究领域:
分析化学
·
脂质组学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Shendong Zhou,Nana Ma,Meijuan Meng,Guangjun Chang,Xiangzhen Shen
Ketosis in dairy cows is often accompanied by the dysregulation of lipid homeostasis in the liver. Acetyl-coenzyme A acetyltransferase 2 (ACAT2) is specifically expressed in the liver and is important for regulating lipid homeostasis in ketotic cows. Lentinan (LNT) has a wide range of pharmacological activities, and this study investigates the protective effects of LNT on β-hydroxybutyrate (BHBA)-induced lipid metabolism disorder in bovine hepatocytes (BHECs) and elucidates the underlying mechanisms. BHECs were first pretreated with LNT to investigate the effect of LNT on BHBA-induced lipid metabolism disorder in BHECs. ACAT2 was then silenced or overexpressed to investigate whether this mediated the protective action of LNT against BHBA-induced lipid metabolism disorder in BHECs. Finally, BHECs were treated with LNT after silencing ACAT2 to investigate the interaction between LNT and ACAT2. LNT pretreatment effectively enhanced the synthesis and absorption of cholesterol, inhibited the synthesis of triglycerides, increased the expression of ACAT2, and elevated the contents of very low-density lipoprotein and low-density lipoprotein cholesterol, thereby ameliorating BHBA-induced lipid metabolism disorder in BHECs. The overexpression of ACAT2 achieved a comparable effect to LNT pretreatment, whereas the silencing of ACAT2 aggravated the effect of BHBA on inducing disorder in lipid metabolism in BHECs. Moreover, the protective effect of LNT against lipid metabolism disorder in BHBA-induced BHECs was abrogated upon silencing of ACAT2. Thus, LNT, as a natural protective agent, can enhance the regulatory capacity of BHECs in maintaining lipid homeostasis by upregulating ACAT2 expression, thereby ameliorating the BHBA-induced lipid metabolism disorder.
研究领域:
兽医学
·
分子生物学
·
药理学
·
动物生理学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2016
·
IF 3.15
Lincai Peng,Hui Li,Yahong Meng
The development of multifunctional cellulose acetate (CA) membranes with enhanced hemocompatibility and antibacterial and antioxidant activities is extremely important for biomedical applications. In this work, significant improvements in hemocompatibility and antibacterial and antioxidant activities of cellulose acetate (CA) membranes were achieved via layer-by-layer (LBL) deposition of chitosan (CS) and water-soluble heparin-mimicking polysaccharides (i.e., sulfated Cantharellus cibarius polysaccharides, SCP) onto their surface. The surface chemical compositions, growth manner, surface morphologies, and wetting ability of CS/SCP multilayer-modified CA membranes were characterized, respectively. The systematical evaluation of hemocompatibility revealed that CS/SCP multilayer-modified CA membranes significantly improved blood compatibility including resistance to non-specific protein adsorption, suppression of platelet adhesion and activation, prolongation of coagulation times, inhibition of complement activation, as well as reduction in blood hemolysis. Meanwhile, CS/SCP multilayer-modified CA membranes exhibited strong growth inhibition against Escherichia coli and Staphylococcus aureus , as well as high scavenging abilities against superoxide and hydroxyl radicals. In summary, the CS/SCP multilayers could confer CA membranes with integrated hemocompatibility and antibacterial and antioxidant activities, which might have great potential application in the biomedical field.
研究领域:
生物材料
·
生物医学工程
·
表面化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
PHYSIOLOGIA PLANTARUM
·
2024
·Vol. 176
·期 1
·e14194
·
IF 5.4
Kai Su,Jing Yuan,Ran Wang,Lanlan Huang,Jian Zhan,Xueqiong Zhang,Shanping Wan,Xinhua He,Hans Lambers,Fuqiang Yu,Yanliang Wang
Ectomycorrhizal (EM) fungi play important roles in nutrient cycling and plant community establishment in forest ecosystems. Effects of EM formation on global alterations of the transcriptome and metabolome during plant-fungal interaction and the key metabolites involved in EM development are largely unknown. Here, dual RNA-Seq and untargeted metabolomic analyses were used to reveal stage-specific and core responses of Pinus yunnanensis and Lactarius deliciosus during mycorrhizal colonization. We found that L. deliciosus colonization in P. yunnanensis roots induced different transcriptional changes across three interaction stages, with a small core of genes consistently regulated at all stages. Concentrations of retinol (vitamin A) and retinoic acid increased while that of B group vitamins decreased during EM formation, which was coordinately regulated by these two plant-fungus partners, with L. deliciosus possibly playing a dominant role. Exogenous retinol altered the diameter and mantle thickness of P. yunnanensis - L. deliciosus EM tips and affected host plant growth and phosphorus acquisition . In the absence of L. deliciosus, exogenous retinol increased the root diameter and the number of root tips of P. yunnanensis . Furthermore, the concentration of auxin increased, but that of abscisic acid decreased during EM formation, and the genes involved in plant hormone signal transduction were gradually activated, and auxin and cytokinin signal transduction potentially played a positive role in this EM symbiosis. In conclusion, we propose that the interaction of P. yunnanensis and L. deliciosus alters vitamin metabolism, which may further affect plant hormone biosynthesis and signal transduction, modulating root morphology and EM traits.
研究领域:
真菌学
·
植物科学
·
分子生物学
·
生态学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）