MOLECULES
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2015
·Vol. 20
·期 9
·16266-16277
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IF 2.416
Zaixiang Lou,Yuxia Tang,Xinyi Song,Hongxin Wang
Screening of anti-biofilm compounds from the burdock leaf based on metabolomics is reported here. The crystal violet assay indicated 34% ethanol elution fraction of burdock leaf could completely inhibit biofilm formation ofPseudomonas aeruginosaat 1 mg·mL−1. Then, the chemical composition of burdock leaf fraction was analyzed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) and 11 active compounds (chlorogenic acid, caffeic acid,p-coumaric acid, quercetin, ursolic acid, rutin, cynarin, luteolin, crocin, benzoic acid, and Tenacissoside I) were identified. Lastly, UPLC-MS analysis was employed to obtain the metabolic fingerprints of burdock leaf fractions before and after inhibiting the biofilm ofPseudomonas aeruginosa. The metabolic fingerprints were transformed to data, analyzed with PLS-DA (partial least squares discriminant analysis) and the peaks whose area was significantly changed were found out. Thus, 81 compounds were screened as potential anti-biofilm ingredients. Among them, rutin, ursolic acid, caffeic acid,p-coumaric acid and quercetin were identified and confirmed as the main anti-biofilm compounds in burdock leaf. The study provided basic anti-biofilm profile data for the compounds in burdock leaf, as well as provided a convenient method for fast screening of anti-biofilm compounds from natural plants.Keywords:screening;anti-biofilm compounds;burdock leaf;metabolomics;data analysis
研究领域:
微生物学
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药用植物学
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DOI
PubMed
高影响因子
Journal of Oleo Science
·
2025
·
IF 1.8
Weijie Shang,Yanjie Huang,Yihang Zhang,Ruojia Li,Liping Dai
Arctium lappa L. (burdock) leaves is effective in the traditional treatment of stroke. Fatty acids are abundant in burdock leaves, and may play an important role in the treatment of stroke. A new procedure was presented that provides 13C NMR-based quantitative measurements of total content, average chain length, average degree of unsaturation, and average polyunsaturation of fatty acids in burdock leaves from five producing areas. The content of five fatty acids in burdock leaves, including α-linolenic acid, linoleic acid, palmitic acid, oleic acid, and stearic acid, were further determined by HPLC-CAD. Among five producing areas, the results of 13C qNMR showed that the content of total fatty acids in burdock leaves ranged from 8.62 to 24.51 μmol·g–1, the average chain length ranged from 19.67 to 23.14, the average degree of unsaturation ranged from 1.19 to 3.91, and the average polyunsaturated moieties ranged from 0.23 to 2.37. The results of HPLC-CAD showed that the contents of α-linolenic acid, linoleic acid, palmitic acid, oleic acid and stearic acid were 0.66 - 6.60 mg·g–1, 0.38-4.41 mg·g–1, 0.46-3.55 mg·g–1, 0.18-0.20 mg·g–1 and 0.10-0.32 mg·g–1 respectively. The developed 13C qNMR method can accurately determine the total fatty acids content and provide relevant information on the fatty acids structure types in burdock leaves. This method is capable of complementing existing fatty acids quantitative methodologies, circumventing their deficiencies, and its application can be extended to other plants.
研究领域:
天然产物化学
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代谢组学
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植物化学
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分析化学
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药用植物学
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PubMed
高影响因子
BIOORGANIC CHEMISTRY
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2022
·
IF 5.307
Hanjing Liang,Qingmei Feng,Hui Guo,Jiangnan Lv,Lingxia Zhang,Qingxia Li,Jun Chi,Qiuyan Liu,Zhimin Wang,Liping Dai
Nine new cadinane-type sesquiterpenoids ( 1 – 9 ) and three new eucalyptane -type sesquiterpenes ( 10 – 12 ) were isolated from the ethyl acetate extract of Burdock leaves, which were commonly used for preventing or treating atherosclerosis in China. Their structures were confirmed by extensive spectroscopic analysis, single-crystal X-ray diffraction analysis and ECD calculations. Compound 1 possessed the rare large conjugated skeleton. All the isolates were evaluated for anti-inflammatory and cholesterol-lowering activities by the LPS- and oxidized-low-density-lipoprotein-stimulated RAW 264.7 cells, respectively. As the results, all isolates could decrease the productions of NO, and down-regulate the accumulation of cholesterol. Among them, 4 showed the most potent cholesterol-lowering effect. For the high content of 4 in the herb, mechanistic study of 4 was performed and the results showed that 4 markedly reduced the release of pro-inflammatory mediators which was probably associated with inhibition of the PI3K/Akt and 5-LOX signaling pathways. The findings of this study demonstrated the anti-inflammatory/cholesterol-lowering effects of the new sesquiterpenes from burdock leaves, which provides chemical basis and scientific evidence for the herb used as anti-atherosclerosis agents for the further study. The sesquiterpene lactones of burdock leaves are expected to become new small molecule inhibitors for the treatment of AS .
研究领域:
药理学
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化学
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分子生物学
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植物科学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）