Journal of Advanced Research
·
2020
·
IF 6.992
Yanwu Chu,Zhanjie Zhang,Qianyuan He,Feng Chen,Ziqian Sheng,Deng Zhang,Honglin Jin,Fagang Jiang,Lianbo Guo
Inorganic or inorganic-organic hybrid nanomaterials have great potential for applications in the biomedical fields. Biological half-life is an essential pharmacokinetic parameter for these materials to function in vivo . Compared to inductively coupled plasma mass spectrometry (ICP-MS), which is the gold standard, laser-induced breakdown spectroscopy (LIBS) is a faster and more efficient elemental detection method. We investigated an efficient way to quantify the metabolic rate using LIBS. Nanoparticle platforms, such as manganese dioxide-bovine serum albumin (MnO 2 -BSA) or boehmite-bovine serum albumin (AlO(OH)-BSA) were injected into mice through intravenous administration for LIBS spectrum acquisition. First, the spectral background was corrected using the polynomial fitting method; The spectral interference was eliminated by Lorentz fitting for each LIBS spectrum simultaneously. The support vector regression (SVR) was then used for LIBS quantitative analyses. Finally, the LIBS results were compared with the ICP-MS ones. The half-lives of MnO 2 -BSA calculated by LIBS and ICP-MS were 2.49 and 2.42 h, respectively. For AlO(OH)-BSA, the half-lives detected by LIBS and ICP-MS were 3.46 and 3.57 h, respectively. The relative error of LIBS is within 5% compared to ICP-MS. The results demonstrate that LIBS is a valuable tool for quantifying the metabolic rates with a high degree of accuracy.
研究领域:
生物医学工程
·
分析化学
·
药代动力学
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DOI
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2023
·
IF 5.1
Jing Wang,Bin Yang,Xinping Yu,Shuji Chen,Wenxin Li,Xu Hong
Understanding the formation of protein corona (PC) is of vital importance for exploring the toxicity of nanoparticles and promoting their safe applications. In this study, CdTe QDs doping with 0, 1%, 5% and 10% Zn were synthesized using one-pot hydrothermal methods . Afterwards, this study explored and compared the formation of pure and Zn doped-QDs PC as well as the subsequent molecular and cellular toxicity. Result found that Zn doping regulated the toxicity of Cd-QDs by controlling their ability to adsorb serum proteins . The adsorption to Cd-QDs induced the dispersion, unfolding, secondary structural changes and the activity loss of bovine serum albumin (BSA). Among the synthesized Cd-QDs, 10%Zn-QDs exhibited the highest fluorescence quantum yield and lowest molecular toxicity. The formations of pure QDs and 10%Zn-QDs with BSA corona are majorly driven by different forces with different patterns. The regulation of BSA on the cytotoxicity differences of pure QDs and 10%Zn-QDs was similar with fetal bovine serum, proving the significant contribution of BSA to the cytotoxicity of Cd-QDs PC. Compared with pure QDs PC, the higher cytotoxicity and oxidative stress level of 10%Zn-QDs PC were correlated with higher intracellular [Cd 2+ ]. Both larger amount of BSA adsorption and higher level of intracellular reactive oxygen species could accelerate the dissolution rates of 10%Zn-QDs and thus result in higher intracellular [Cd 2+ ].
研究领域:
纳米技术
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毒理学
·
生物化学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
Artificial Cells Nanomedicine and Biotechnology
·
2017
·
IF 5.605
Wenping Wang,Yaya Lei,Hong Sui,Wenping Zhang,Rongyue Zhu,Jun Feng,Hong Wang
The aim of the present study was to prepare and evaluate microparticle formulation encapsulated with glycyrrhetinic acid (GA) based on bovine serum albumin (BSA). The drug-loaded nanoparticles were firstly formed by a simple desolvation method, and were further assembled into microparticles using zinc chloride and glutaraldehyde as crosslinkers. The obtained microparticles contained approximately 30% (w/w) drug and showed as spherical particles with a size of about 2 μm. Differential scanning calorimetry (DSC) and X-ray powder diffraction (XRPD) analysis indicated that GA lost its crystallinity during the nano/microencapsulation process. In vitro dissolution study demonstrated a typical sustained-release pattern for 24 h with a burst of 28.1% at the first 30 min, which fitted well by Higuchi model. After intravenous administration into mice, the microparticle formulation remained a higher drug level than the solution formulation in blood and liver for more than 18 h. These results suggested the potential benefit of using the prepared albumin microparticles as a promising vector for enhanced liver delivery of poorly water-soluble drug.
研究领域:
药剂学
·
生物医学工程
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DOI
PubMed
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Shu Chen,Weiwei Yu,Guangnan Xing,Zhiguang Song,Guodong Feng
Cysteine (Cys) is one of the most basic mercaptans in the human body. As an important endogenous small molecule mercaptan, Cys plays a vital role in various physiological processes and can participate in maintaining redox balance to ensure homeostasis. Abnormal Cys levels can lead to a variety of diseases. However, the detection of cysteine may be interfered with by other small molecule biothiols. Therefore, the design of fluorescent probes based on the structural characteristics and reactivity of cysteine has become the focus of current research. In this paper, a fluorescent probe (3-(2H-benzo[d][1,2,3]triazol-2-yl)-2-oxo-2H-benzo[g]chromen-8-yl acrylate, BTAB) for Cys detection was synthesized with acrylic ester as the reaction site. Under the conditions of gradual optimization, BTAB can achieve selectivity and anti-interference ability for Cys detection. The linear range of Cys was 0.3–10 μM, and the detection limit was 0.154 μM. Finally, this probe was applied to detect the Cys content in bovine serum samples with satisfactory results.
研究领域:
生物化学
·
医学检测
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DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2022
·
IF 11.467
Chencheng Xue,Hui Zhang,Xuan Wang,Haoyu Du,Lu Lu,Yang Fei,Yanan Li,Yuchen Zhang,Menghuan Li,Zhong Luo
Nanotechnology-enabled ferroptosis therapy is an emerging paradigm for tumor treatment, but amplifying ferroptotic damage in tumor cells in a safe and selective manner is still challenging, which severely hinders its clinical translation. In this study, we constructed a bio-inspired protein nanocomplex based on natural-occurring bovine serum albumin (BSA) and ferritin for efficient tumor elimination via cooperatively enhanced ferroptosis therapy. The long-circulating BSA molecules provided multiple anchoring points for the efficient loading of the GPX4-inhibiting ferroptosis inducer (1S, 3R) RAS-selective lethal 3 (RSL3), which was further complexed with ferritin via acidity-responsive glutaraldehyde linkers. The ferritin moieties may not only bind to transferrin receptor 1 overexpressed on tumor cell membrane for targeted endocytic uptake but also be degraded in lysosomes to induce iron overload, which could substantially promote the lipid peroxidation in tumor cells and cooperate with the glutathione peroxidase 4 (GPX4)-inhibiting capability of RSL3 to induce pronounced ferroptosis. The in vitro and in vivo results collectively demonstrated that the albumin-ferritin-based nanocomplex could present superior antitumor effects with no obvious adverse effects, which may open new avenues for the clinical translation of ferroptosis-dependent therapeutic modalities.
研究领域:
肿瘤学
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纳米医学
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生物材料学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2024
·
IF 5.9
Shi-Ya Peng,Ya-Di Yang,Rong Tian,Naihao Lu
A key characteristic to be elucidated, to address the harmful health risks of environmental perfluorinated alkyl substances (PFAS), is their binding modes to serum albumin, the most abundant protein in blood. Hexafluoropropylene oxide-dimer acid (GenX or HFPO-DA) is a new industrial replacement for the widespread linear long-chain PFAS. However, the detailed interaction of new-generation short-chain PFAS with albumin is still lacking. Herein, the binding characteristics of bovine serum albumin (BSA) to GenX were explored at the molecular and cellular levels. It was found that this branched short-chain GenX could bind to BSA with affinity lower than that of legacy linear long-chain perfluorooctanoic acid (PFOA). Site marker competitive study and molecular docking simulation revealed that GenX interacted with subdomain IIIA to form BSA-GenX complex. Consistent with its weaker affinity to albumin protein, the cytotoxicity of branched short-chain GenX was less susceptible to BSA binding compared with that of the linear long-chain PFOA. In contrast to the significant effects of strong BSA-PFOA interaction, the weak affinity of BSA-GenX binding did not influence the structure of protein and the cytotoxicity of GenX. The detailed characterization and direct comparisons of serum albumin interaction with new generation short-chain GenX will provide a better understanding for the toxicological properties of this new alternative.
研究领域:
生物化学
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毒理学
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环境科学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
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IF 3.232
Quan-Quan Zhuang,Shao-Bin He,Kai-Yuan Huang,Hua-Ping Peng,Chang-Mai Chen,Hao-Hua Deng,Xing-Hua Xia,Wei Chen,Guo-Lin Hong
Various types of bovine serum albumin (BSA)-protected fluorescent gold nanoclusters (BSA-AuNCs) have been fabricated and applied in various fields. However, the conventional synthesis methods for BSA-AuNCs usually yield a low photoluminescence quantum yield (PLQY) in solution. In this study, we systematically examined the influences of incubation time, temperature, and pH on the formation process of BSA-AuNCs and then developed a novel strategy to synthesize BSA-AuNCs with PLQY (26%), far exceeding that of existing counterparts. Of the three important factors, pH, temperature, and time, pH plays a key role in the formation of BSA-AuNCs with different compositions and fluorescence properties. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) results showed that BSA-Au 20 NCs with high purity can be produced at a pH value of 10 and the correct combination of incubation temperature and reaction time. The advantages of the obtained BSA-Au 20 NCs, including small size, high PLQY, long lifetime, high purity, as well as facile modification, make them ideal candidates for luminescent probes in imaging and sensing applications.
研究领域:
生物化学
·
纳米科技
·
材料科学
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DOI
PubMed
高影响因子
Carbon Letters
·
2023
·Vol. 33
·期 7
·1935-1945
·
IF 4.5
Wang Qian,Wang Miao,Zheng Lufei,She Yongxin,Wang Jing,Jia Minghong,Abd El-Aty A. M.
The widespread and extensive use of glyphosate in agriculture has raised concerns about its potential impact on the quality and safety of agricultural products. Conventional detection methods require long analysis times, making them impractical for the rapid detection of large quantities of samples. Therefore, developing a fast and simple detection system for glyphosate pesticide residues is urgent. In this study, the development of a facile fluorescence probe synthesized using a simple one-pot hydrothermal method for the determination of glyphosate is an important step toward addressing the need for a fast and simple detection system. The present sensor was created using bovine serum albumin (BSA) as a precursor, and the sensor operates by producing an “off–on” fluorescent signal. The bovine albumin-derived BSA-CDs emitted light yellow fluorescence, but this fluorescence was quenched (or suppressed) by the presence of Cu 2+ ions. However, the fluorescence can be restored by the presence of glyphosate, which interacts with the Cu 2+ ions to form a complex and release the BSA-CDs from suppression. The functional groups in glyphosate can capture Cu 2+ and break the BSA-CDs/Cu 2+ combinatorial system. The BSA-CDs/Cu 2+ fluorescence quenching system had good selectivity for glyphosate. The detection limit of the BSA-CD/Cu 2+ fluorescence sensor was 0.05 µg/mL. This developed method was utilized to successfully detect glyphosate in Chinese wheat. The average recoveries ranged from 98.9 to 100.7%, with a relative standard deviation < 3.0%, showing good prospects for practical applicability.
研究领域:
农业科学
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分析化学
·
环境科学
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2017
·
IF 4.233
Lu Lai,Xiao-Qian Wei,Wei-Hua Huang,Ping Mei,Zhao-Hua Ren,Yi Liu
The effects of carbon quantum dots (CQDs) on the dynamic properties of bovine serum albumin (BSA) were investigated using pendant drop profile analysis method. Moreover, the effects of CQDs on the competitive adsorption of BSA and dipalmitoyl phosphatidylcholine (DPPC) were examined. CQDs reduce the fluorescence intensity of BSA and cause a red shift in fluorescence emission . The quenching constant at pH 4.3 is almost twice as large as that of the value obtained at pH 6.0. A small amount of CQDs does not influence the dynamic surface adsorption properties of BSA molecules. As the CQD concentration increases, a gradual increase in adsorption rate of BSA molecules is observed. Moreover, the addition of CQDs results in a significant transition of kinetic dependencies of surface elasticity of BSA solution when the CQD concentration exceeds a critical value. The appearance of the maximum surface elasticity value is probably attributed to the formation of tails and loops. When the dynamic surface properties are dominated by BSA molecules, the effects of CQDs on the surface properties of BSA/DPPC mixture are similar to those of BSA alone. However, when the surface film mainly consists of DPPC, CQDs can obviously change the interfacial properties of DPPC monolayer.
研究领域:
生物物理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 5
·909-915
·
IF 3.719
Zhong Yaping,Zhu Jingjing,Wang Qingpu,He Yu,Ge Yili,Song Congwu
We show that copper nanoclusters coated with bovine serum albumin are viable probes for fluorometric determination of Cu(II) ion. The effect is attributed to the paramagnetic nature of Cu(II) bound to BSA. The ions Hg(II) and Fe(III) also give a strong effect but can be discerned by addition of EDTA. The probe can be regenerated by adding an excess of EDTA. The method is capable of detecting Cu(II) in the 0.02–34 μM, and the limit of detection is 1nM. The method was applied to the determination of Cu(II) in spiked water samples. It is rapid, simple, and fairly sensitive. The method may also be applied to detect Hg(II) and Fe(III) if Cu(II) is absent.
BSA-coated copper nanoclusters (NCs) are added to the sample, and EDTA is then added to block potentially interfering ions. The fluorescence of the NCs increases with increasing concentration of Cu(II) ions being present.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Molecular Catalysis
·
2020
·
IF 3.687
Jiajun Dai,Yunlong Guo,Luhang Xu,Guilin Zhuang,Yi Zheng,Daohua Sun,Jiale Huang,Qingbiao Li
Bovine serum albumin (BSA)-templated CeO 2 was prepared by citrate sol-gel method and used as a support to synthesize Au catalyst for benzene oxidation. It was found that the Au/BSA-CeO 2 with 3.2 nm Au nanoparticles (NPs) gave rise to superior catalytic performance for benzene oxidation in which the temperature for 90 % benzene conversion was only 210 °C. In addition, the catalytic activity of Au/BSA-CeO 2 remained stable for 140 h of on-stream reaction. Besides, density functional theory calculation demonstrated how the porous structure of CeO 2 influenced the oxygen vacancy generation, and the synergetic effects of Au NPs and CeO 2 support facilitated the activation of benzene which was adsorbed on Au NPs prior to benzene oxidation. The catalysis of benzene oxidation over Au/CeO 2 catalyst follows the dual-site mechanism. BAS-templated CeO 2 support possessed hierarchical structure, abundant adsorbed oxygen species and synergic interaction with Au that determined the performance of Au/BSA-CeO 2 .
研究领域:
催化化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2024
·
IF 14.3
Wentai Zhang,Jiangling Zhang,Fangkun Hu,Wenxuan Wang,Zeyu Du,You Ke,Qing Ma,Xiaohui Mou,Jing Lu,Zhilu Yang
Universal protein coatings have recently gained wide interest in medical applications due to their biocompatibility and ease of fabrication. However, the challenge persists in protein activity preservation, significantly complicating the functional design of these coatings. Herein, an active dual-protein surface engineering strategy assisted by a facile stepwise protein–protein interactions assembly (SPPIA) method for catheters to reduce clot formation and infection is proposed. This strategy is realized first by the partial oxidation of bovine serum albumin (BSA) and lysozyme (LZM) for creating stable nucleation platforms via hydrophobic interaction, followed by the assembly of nonoxidized BSA (pI, the isoelectric point, ≈4.7) and LZM (pI ≈11) through electrostatic interaction owing to their opposite charge under neutral conditions. The SPPIA method effectively preserves the conformation and functionality of both BSA and LZM, thus endowing the resultant coating with potent antithrombotic and bactericidal properties. Furthermore, the stable nucleation platform ensures the adhesion and durability of the coating, resisting thrombosis and bacterial proliferation even after 15 days of PBS immersion. Overall, the SPPIA approach not only provides a new strategy for the fabrication of active protein coatings but also shows promise for the surface engineering technology of catheters.
研究领域:
生物医学工程
·
材料科学
·
表面工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）