EUPHYTICA
·
2024
·Vol. 220
·期 4
·1-13
·
IF 1.6
Zhong Yuan,Du Ming-jie,Ji Run-ze,Rong Qi,Guan Zi-heng,Cheng Fang-yun
Cross-incompatibility is the major challenge in inter-sectional distant hybridization between tree peonies ( Paeonia sect. Moutan ) and herbaceous peonies ( Paeonia sect. Paeonia ). Most of the intersectional cross combinations rarely produce hybrid progenies or even seeds. However, a combination with Paeonia × lemoinei Rehd. ( P. delavayi × P. suffruticosa , tree type) as the pollen parent and P. lactiflora (herbaceous) as the seed parent resulted in many progenies. This research focuses on cytogenetics of two highly compatible intersectional combinations, P. lactiflora ‘Martha W.’ × P. × lemoinei ‘Golden Era’ (‘MW’ × ‘GE’) and P. lactiflora ‘Fen Yun Fei He’ × P. × lemoinei ‘L’Esperance’ (‘FYFH’ × ‘LE’). Karyotype analysis combined with genomic in situ hybridization (GISH) showed that the seed parents, ‘MW’ and ‘FYFH’, are both diploids (2 n = 2 x = 10, CC), while the pollen parents, ‘GE’ and ‘LE’, are tetraploid (2 n = 4 x = 20, AABB) and diploid (2 n = 2 x = 10, AB) respectively. Four progenies from ‘MW’ × ‘GE’, and another four progenies from ‘FYFH’ × ‘LE’, are all triploids (2 n = 3 x = 15, ABC). P. × lemoinei contributes two sets of chromosomes (AB) showing distinct signals in all tested triploids. The essential of a successful intersectional hybridization is to obtain 2 x gametes from tree peony. P. × lemoinei has better compatibility in intersectional hybridization than other tree peony species, probably because of its higher frequency of 2 x gamete (unreduced in diploid, or reduced in tetraploid) generation. Tetraploid tree peonies, such as ‘GE’, might produce abundant 2 x gametes through normal meiosis, which is the possible reason for their better fertility and superior intersectional compatibility than diploid tree peonies. The mechanism of compatibility in intersectional hybridization is supposed to be a rebalance between the distant divergent parents, tree peonies and herbaceous peonies, triggered by ploidy increase of gametes from tree peonies. The discovery of tetraploid tree peony also provides valuable materials to study the mechanism of polyploidization in sect. Moutan , and to promote the distant hybridization breeding in Paeonia.
研究领域:
植物学
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遗传学
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植物育种
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DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 9
·3000
·
IF 4.927
Xiao Yu,Huaibi Zhang,Juan Wang,Junming Wang,Zhenxing Wang,Jinbo Li
Essential oils were extracted from dark-purple, red and yellow petals ofPaeonia delavayiusing Supercritical Carbon Dioxide method. The compositions of essential oils were analyzed using gas chromatography-mass spectrometry (GC-MS). Antioxidant activity assays were carried out using DPPH, ABTS- and FRAP methods. Total polyphenols and total flavonoids were measured to evaluate the in vitro antioxidant activity in addition to the volatile compounds contained in the essential oils extracted from the flower petals ofP. delavayiwith the three flower colors. A total of 194 compounds were detected from essential oils ofP. delavayiflowers, including 83 in dark-purple petals, 90 in red petals and 80 in yellow petals. These compounds mainly include alcohols, aldehydes, ketones, alkenes, alkanes, esters and polyphenols. The results showed that the volatile compounds accumulated differentially among the essential oils from the different colors of flower petals. Principal component analysis (PCA) indicated that essential oils derived from dark-purple and red petals were more closely clustered while the yellow petal essential oil was very different with both the purple-red and red. Antioxidant assays suggested that the radical scavenging activity and the iron reduction antioxidant activity in the essential oils were highly correlated with the flower petal colors. These results suggestP. delavayiflower petals are potentially good resources for high quality essential oils and natural antioxidants.Keywords:Paeonia delavayi;flower colors;essential oil;antioxidant activity
研究领域:
植物学
·
药理学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 12
·3268
·
IF 3.098
Gaoming Lei,Jie Li,Tao Zheng,Junqiao Yao,Jingjing Chen,Lengxin Duan
Paeonia suffruticosaAndr. is a famous ornamental and aromatic plant with hundreds of cultivars in China. The objective of this work was to investigate comparative chemical profiles of essential oils and hydrolate extracts from eightP. suffruticosaAndr. cultivars from Central China. The percentages of hydrocarbons in hydrolate extracts (≤1.1%) were significantly lower than those in the essential oils (29.8–63.7%). The percentages of oxygenated compounds in hydrolate extracts (98.3–99.8%) were significantly higher than those in the essential oils (34.8–69.6%). Multivariate analyses with hierarchical clusters and principal components further indicated the chemical differences between essential oils and hydrolate extracts. Due to predominance of oxygenated compounds and almost trace level of hydrocarbons,P. suffruticosaAndr. hydrolate extracts could be good alternatives to the essential oils. Moreover, distribution of major oxygenated compounds in hydrolate extracts varied with cultivars. Hydrolate extracts from ’SHT’, ’WLPS’ and ’BXT’ presented chemotypes of methylated phenols (65.0%), 2-phenylethanol (64.4%) and geraniol + citronellol + nerol (59.9%), respectively. Those from five other cultivars presented somewhat mixed chemotypes. These results were further confirmed by quantitative evaluation relative to the major oxygenated compounds. The outcome of this work will promote applications ofP. suffruticosaAndr. hydrolate extracts in fragrances and cosmetics.Keywords:Paeonia suffruticosaAndr.;essential oil;hydrolate extract;chemical profile;GC-MS;GC-FID
研究领域:
植物学
·
化学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Weiwei Wu,Zhiqiang Zheng,Zhihui Wang,Biyun He,Siqing Du,Wen Zeng,Weijiang Sun
White Peony Tea (WPT) is valued for its unique flavor and pleasant sensory effects. However, the specific aroma compounds in WPT contributing to this pleasant sensation and the underlying mechanisms remain unclear. This study integrates GC–MS, computational modeling, and sensory evaluation to systematically explore the key pleasant aroma compounds in WPT and their potential mechanisms. Seven key components were identified as eliciting the pleasant sensation of WPT, including cedrol and β -ionone. These compounds activate human pleasure receptors, influencing neurological pathways to enhance sensory pleasure. Molecular simulations validated the stability of the interactions between the aroma compounds and their targets, specifically cedrol–CHRM4, cedrol–ADORA1, β -ionone–ADORA2A, and cedrol–MAOB. Sensory evaluation revealed pleasantness scores for these aroma compounds between 2.60 and 6.80, supporting the positive effects on sensory experience. This research provides scientific insight into the relationship between tea aroma and sensory pleasure, providing a foundation for the development of tea products.
研究领域:
食品科学
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神经生物学
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计算化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Fang Zhang,Jie Qu,Kiran Thakur,Jian-Guo Zhang,Andrei Mocan,Zhao-Jun Wei
The present study demonstrated the hydrolysis of peony seed protein isolate (PSPI) by using alcalase and resulted in the generation of an anti-oxidative peptide. In brief, a model was used to illustrate the enzymolysis of PSPI with the determination of kinetic factors as per investigation information. The model proved suitable to explain the PSPI hydrolysis by alcalase. A novel anti-oxidative peptide was obtained successfully by ultrafiltration and a series of chromatography techniques. Subsequently, a purified fragment was identified with the amino acid sequence of SMRKPPG followed by its synthesis and evaluation of its anti-oxidative activities. After hydrolysis, the peony seed protein hydrolysate (PSPH) with the degree of hydrolysis of 18% displayed the most significant antioxidant action which was further used to isolate the anti-oxidative peptide.
研究领域:
植物化学
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食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 17
·3184
·
IF 5.2
Weiqi Fu,Jiamin Ren,Shuwen Li,Dirong Ren,Xixi Li,Chenghuan Ren,Xueru Zhao,Jiaying Li,Fengjuan Li
Peony seed meal (PSM) is the by-product obtained from peony seeds after oil extraction. In this study, PSM was incorporated into traditional koji-making, and its impacts on koji enzyme activities and flavor compounds in final products were investigated. In the process of koji fermentation, the optimal addition ratio of PSM to soybean was determined as 7:3. Under this ratio, the maximum enzyme activities of neutral protease, amylase, and glucoamylase were 1177.85, 686.58, and 1564.36 U/g, respectively, and the koji obtained was subjected to maturation. During post-fermentation, changes in the fermentation characteristics of the paste samples were monitored, and it was found that compared to the soybean paste without PSM, the enzyme activities maintained at a relatively good level. The PSM soybean paste contained a total of 80 flavor compounds and 11 key flavor compounds (OAV ≥ 1), including ethyl isovalerate, isovaleric acid, hexanal, phenylacetaldehyde, 3-Methyl-1-butanol 4-heptanone, 2-pentylfuran, methanethiol ester caproate, isoamyl acetate, 3-methyl-4-heptanone, and isovaleraldehyde. These findings could be used to improve the quality of traditional fermented paste, enrich its flavor, and simultaneously promote PSM as a valuable resource for fermented foods.Keywords:peony seed meal;fermented soybean paste;enzyme activity;koji making;flavor compounds
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND BIOPRODUCTS PROCESSING
·
2024
·
IF 3.5
Ruixue Wang,Jiayi Yang,Yaqing Fan,Yulu Wang,Chuang Xu,Guoyong Guo,Zhanzhong Wang
The effectiveness of oil-in-water (O/W) microemulsion (ME) in enhancing the oxidation and the storage stability of peony seed oil (PSO), its fatty acid mixtures (FAM-1, FAM-2), and α-linolenic acid (ALA) were investigated in this work. Storage stability was assessed by measuring iodine value , acid value, and fatty acid composition under varying temperature conditions devoid of oxygen. Additionally, antioxidant stability of both the oil phase and ME was evaluated by measuring the peroxide value , with oxidation kinetics modeled using the Arrhenius equation to predict shelf life. The results demonstrated that the change of iodine values for PSO, FAM-1, FAM-2 and ALA at 4, and -20 °C for 90 days was not obvious. At 25℃, the ALA content in PSO, FAM-1, FAM-2 and ALA samples decreased by 4.81%, 3.94%, 2.86%, and 0.12% after 90 days. Therefore, low temperature without oxygen environment is more suitable for the storage of oil and fatty acid. Embedding PSO in ME can effectively control the generation rate of peroxides. At 4°C, the shelf life of PSO, FAM-1 and FAM-2 encapsulated in MEs was prolonged by 28.36%, 9.89% and 4.24%, respectively. Thes results confirm that O/W ME can improve the oxidation stability and prolong the storage period of fat-soluble compounds. These findings provide valuable insights into enhancing the oxidative stability of PSO and its fatty acids, thereby facilitating the development of ME products loaded with fatty acids in the future.
研究领域:
食品科学
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植物化学
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产品关联
DOI
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-11
·
IF 5.228
Jiao Jiajia,Sun Lili,Guo Zaiyu,Hou Sen,Holyst Robert,Lu Yun,Feng Xizeng
Polydimethylsiloxane (PDMS) is widely used as a cell culture platform to produce micro- and nano-technology based microdevices. However, the native PDMS surface is not suitable for cell adhesion and is always subject to bacterial pollution and cancer cell invasion. Coating the PDMS surface with antibacterial or anticancer materials often causes considerable harm to the non-cancer mammalian cells on it. We have developed a method to fabricate a biocompatible PDMS surface which not only promotes non-cancer mammalian cell growth but also has antibacterial and anticancer activities, by coating the PDMS surface with a Chinese herb extract, paeonol. Coating changes the wettability and the elemental composition of the PDMS surface. Molecular dynamic simulation indicates that the absorption of paeonol onto the PDMS surface is an energy favourable process. The paeonol-coated PDMS surface exhibits good antibacterial activity against both Gram-positive and Gram-negative bacteria. Moreover considerable antibacterial activity is maintained after the coated surface is rinsed or incubated in water. The coated PDMS surface inhibits bacterial growth on the contact surface and promotes non-cancer mammalian cell growth with low cell toxicity; meanwhile the growth of cancer cells is significantly inhibited. Our study will potentially guide PDMS surface modification approaches to produce biomedical devices.
研究领域:
生物医学工程
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材料科学
·
细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Fengli Chen,Xinyu Yang,Yibo Ma,Xiaoxia Wang,Duqiang Luo,Lei Yang,Yongfan Yang
Silver magnetic amino silicone adhesive (Fe 3 O 4 @SiO 2 @NH 2 @Ag) particles were prepared for the purification of α-linolenic acid from tree peony seed oil under applied magnetic field. First, Fe 3 O 4 @SiO 2 @NH 2 @Ag particles were prepared and physicochemically characterized, including XRD, TG, FTIR, SEM, magnetic hysteresis curves and elemental analysis. The static process for the purification of α-linolenic acid using Fe 3 O 4 @SiO 2 @NH 2 @Ag particles was investigated, including adsorption curve, desorption curve, elution solvent composition and adsorption isotherm. The result indicated that 0-1–4% acetone- n -hexane elution solvent was selected for the gradient elution process, 2 h and 60 min were the time required to reach adsorption and desorption equilibrium, 20 °C was selected as the adsorption temperature, Langmuir model was suitable to fit and explain the equilibrium data, and the adsorption process was spontaneous and exothermic. Under applied magnetic field, the dynamic process for the purification of α-linolenic acid using Fe 3 O 4 @SiO 2 @NH 2 @Ag particles was investigated, and the optimum conditions were 20:1 µL/g loading amount, 0.5 mL/min flow rate and 51.73 Oe magnetic field intensity. After purification, the purity and recovery ratio of α-linolenic acid were calculated to be 94% and 74%, respectively. Furthermore, the recycled Fe 3 O 4 @SiO 2 @NH 2 @Ag particles still achieved better purification result. Therefore, the developed method shows a good application prospect in the field of separation and purification of α-linolenic acid.
研究领域:
化学工程
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Shenglin Wang,Jiahui Wei,Hanwen Ge,Zexu Yan,Mingzhe Jiang,Jiale Lu,Meixian Pu,Bin Li,Huanfei Xu
Microwave-assisted deep eutectic solvent extraction (MAE-DES) method was proposed to extract of active compounds from peony petals. Four different components of DES were used as extraction solvent, and the extraction effect was evaluated by total phenolic content (TPC), total flavonoid content (TFC) and total anthocyanin content (TAC). Response surface methodology (RSM) was used to optimize the reaction temperature, reaction time and liquid–solid ratio. The results showed that under optimized conditions, the extraction yields of TPC, TFC and TAC were 321.59 mg GAE/g, 61.65 mg RE/g and 2.15 mg C3GE/g, respectively. The characterization of solid residue showed that DES effectively disrupted the structure of peony petals. In addition, the importance of variables affecting extraction efficiency was investigated by machine learning (ML). Finally, the extraction mechanism of DES for active compounds was explored by density functional theory (DFT) and molecular dynamics (MD) simulation. The results showed that the extraction efficiency of active compounds was affected by the hydrogen bond interaction with DES. This study provides efficient extraction protocol and mechanism research for the extraction of active compounds from multiple methods and insights.
研究领域:
植物化学
·
计算生物学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 94
·期 7
·2229-2237
·
IF 2.659
Xiaoqi Yi,Xiaojing Mu,Gang Chen,Yan Gou,Chengshan Wang,Xiaolin Yuan
Background Aqueous two-phase extraction has advantages of green extraction, high yield, good product purity and low cost. However, conventional aqueous two-phase systems (ATPSs) are limited in extraction/separation due to high polarity. Some researchers tuned the hydrophobicity of ATPSs by addition of hydrophobic ionic liquids or polymers. We aimed to tailor the hydrophobicity of a solvent–salt–water ATPS using cosolvent for extraction of paeonol from cortex moutan . Results Tie-line length and compositions in the ATPS were employed for qualitative evaluation of polarity and polarity difference between the two phases. Conditions such as pH, salt type and concentration, total solvent volume fraction and cosolvent fraction were optimized. Ethyl acetate (EA) as cosolvent in ethanol–NaH 2 PO 4 –H 2 O (EtPO) system did not alter the first-order kinetic extraction model, but it improved the kinetic constant and boosted the partition coefficient from 36.7 to 58.8. For both EtPO and EtPO–EA systems, both the extraction yields and partition coefficients increased when temperature increased from 11 to 37 °C. The optimized EtPO–EA system demonstrated higher extraction yield than EtPO and 70% (v/v) ethanol systems by 25.4 and 19.5%, respectively. Conclusions The hydrophobicity of an ATPS was successfully tuned with cosolvent for paeonol extraction. The cosolvent–salt–water two-phase systems are promising in extraction of weakly polar components from herbs. © 2019 Society of Chemical Industry
研究领域:
化学工程
·
药学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Essential Oil Bearing Plants
·
2020
·
IF 0.824
Gaoming Lei,Chaoying Song,Erqiang Wang,Senming Zhao,Cheng Yang
The use of a mixed-salt substrate (sodium chloride-choline bromide-1-ethyl-1-methyl-pyrrolidinium bromide; molar ratio: 1:1:1) has been proposed for hydrodistillation of essential oils. Tree peony ‘Taiping Hong’ (Paeoniaceae), a cultivated essential oil-bearing plant, was investigated. After using the salt substrate, distillation time decreased from 140 to 40 min, essential oil yield increased from 0.48 % to 0.69 % and the pseudo-first-order rate constant increased from 0.0233 to 0.123 min-1. Potential mechanisms are (1) osmosis effect and hydrogen-bond interactions, which improve cell wall rupture; (2) the increase in polarity of aqueous distillation media, which prevents emulsification. The essential oil composition was influenced by an increase in oxygenated compound content (32.9 % to 49.0 %) and a decrease in hydrocarbon content (65.3 % to 49.6 %). Furan linalool oxide (cis and trans), linalool and α-terpineol contents increased by 400 %, 250 % and 200 %, respectively. (E,E)-α-farnesene, α-muurolene and octadecane contents decreased by 50 %, 46 % and 38 %, respectively. Possible mechanisms are: (1) improved cell rupture, which significantly facilitates extraction of oxygenated compounds; (2) hydrogen bonding with oxygenated compounds, which enhances their transfer from the inside of cells to bulk solution; (3) salting-out effect, which decreases dissolution of oxygenated compounds and improves their distillation. The essential oil obtained was mainly composed of phloroglucinol trimethyl ether (21.9 %), nerol (10.2%), tricosane (9.1 %), nonadecane (9.0 %), α-muurolene (5.0 %) and furan linalool oxides (3.0 %). Target prediction analysis indicated that it has potential applications in aromatherapy. In conclusion, the use of the mixed-salt substrate combined with hydrodistillation can result in efficient isolation of essential oils.
研究领域:
植物化学
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药用植物学
·
芳香疗法
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）