Food Bioscience
·
2024
·
IF 4.8
Ran Du,Wan Li,Jiawei Li,Shan Zeng,Zhongqin Chen,Jialong Gao,Huina Zheng,Haisheng Lin,Guoping Zhu,Xiaoming Qin,Wenhong Cao
Oyster hydrolysates exhibit promising potential as a zinc supplement for its significant amounts of zinc, however, the zinc chemical speciation which determines zinc nutrition in oyster hydrolysates has not been investigated. This study optimized the oyster hydrolysis conditions using the Response surface methodology (RSM) method, and the zinc chemical speciation, the sequence of zinc-containing peptides, and the binding sites of zinc and peptides were further investigated. Results showed that inorganic and organic zinc in oysters were 60.15% and 36.53% of total zinc respectively, while organic zinc in oysters aqueous extract was 4.54% of which 3.56% was present in the polysaccharide fraction. The zinc yield was 84.20% of total zinc under optimal hydrolysis conditions, while the organic zinc increased to 52.93% of which 38.14% existed in the protein/peptides fraction. Zinc in protein/peptide fraction primarily was bound to low-molecular-weight peptides (Mw < 1000 Da). Further, 42 shared zinc-containing peptides (mainly 3–4 peptides) were identified. Molecular docking indicated that Glu, Leu , and Tyr might be the binding sites for zinc and peptides. These findings provided a theoretical basis for the development of novel and safe zinc dietary supplements.
研究领域:
食品科学
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营养学
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生物化学
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海洋生物学
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DOI
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 16
·2755
·
IF 5.719
Yue Xu,Huan Liao,Jing Zhang,Haijun Lu,Xinghua He,Yi Zhang,Zhenbin Wu,Hongyu Wang,Minghua Lu
Recovery phosphorus (P) from P-contaminated wastewater is an efficient and environmentally friendly mean to prevent water pollution and alleviate the P shortage crisis. In this study, oyster shell as calcium sources and peanut shells as carbon sources (mass ratio 1:1) were used to prepare a novel Ca-modified biochar (OBC) via co-pyrolysis, and its potential application after P adsorption as a P biofertilizer for soil was also investigated. The results shown that OBC had a remarkable P adsorption capacity from wastewater in a wide range of pH 4–12. The maximum P adsorption capacity of OBC was about 168.2 mg/g with adsorbent dosage 1 g/L, which was about 27.6 times that of the unmodified biochar. The adsorption isotherm and kinetic data were better described by Langmuir isotherm model (R2> 0.986) and the pseudo second-order model (R2> 0.975), respectively. Characterization analysis of OBC before and after P adsorption by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and specific surface area and porosity analyzer (BET) indicated that the remarkable P adsorption capacity of OBC was mainly ascribed to chemical precipitation, electrostatic adsorption, and hydrogen bonding. Pot experiment results showed that OBC after P adsorption could significantly promote the germination and growth ofSpinacia, which manifested that OBC after P adsorption exhibited a good ability to be reused as P fertilizer for soil.Keywords:phosphorous;oyster shell;peanut shell;biochar;P biofertilizer
研究领域:
环境科学
·
农业科学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Xiaojiao Dai,Ziling Huang,Chuanyi Liu,Zhigang Li,Qingmin Chen,Yi Xu,Xiaomei Chen,Quansheng Chen,Jie Wei
Adenosine triphosphate (ATP) levels are recognized as a critical indicator of microbial content in food safety. The development of highly efficient ATP-sensing methods is essential for assessing food freshness. Herein, we propose a simple-yet-efficient hemin/G-quadruplex (G4) DNAzyme-based aptasensor for ATP detection and oyster freshness assessment. The anti-ATP aptamer subunits specifically bind to ATP, leading to self-assembly of the hemin/G4 DNAzyme. The newly produced DNAzyme then catalyzes the oxidation of ABTS 2- to produce the colored ABTS •− , providing an absorbance response for sensitive ATP analysis. The aptasensor facilitates high-performance and accurate determination of ATP with a low detection limit of 0.065 μM. Furthermore, this sensor enables reliable ATP analysis in oyster samples and evaluation of oyster freshness. In particular, a good linear correlation was established between microbial content and ATP levels. The proposed aptasensor for the assessment of freshness is characterized by its simplicity and efficiency, potentially offering a new tool to monitor food safety.
研究领域:
生物化学
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食品安全
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分子生物学
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DOI
高影响因子
WATER ENVIRONMENT RESEARCH
·
2022
·Vol. 94
·期 4
·e10714
·
IF 3.306
Yao Shi,Yifei Xing,Zhilian Song,Xueming Dang,Huimin Zhao
Fe–Mn binary oxide (FMBO) is a promising adsorbent for As(III) removal through combined adsorption and oxidation. The calcined oyster shell-supported Fe–Mn binary oxide (FMBO/OS) adsorbent was synthesized by the co-precipitation method. Results indicated that the calcined oyster shell, as a carrier, improved the stability of FMBO and its adsorption capacity for As(III). The maximum adsorption capacity of FMBO/OS on As(III) reached 140.5 mg·g −1 . Under pH 5.0 and 25°C, the removal efficiency of FMBO/OS to As(III) solution (C 0 = 10 mg·L −1 ) reached 87% within 12 h. Moreover, based on the characterization analyses, the removal mechanisms of As(III) were deduced to include the combined adsorption and oxidation process of FMBO and the synergistic effect of oyster shells. This work provides new insights into synthesizing efficient and green adsorbents to remove aqueous As(III). Meanwhile, it provides technical support for reusing waste biomass materials such as the oyster shell. Practitioner Points FMBO/OS was prepared by a simple hydrothermal co-precipitation method. The carrier alleviates the agglomeration of Fe–Mn oxides. The adsorbent shows a strong adsorption capacity of As(III) and good selectivity. The good results benefit from the synergistic effect of calcium arsenate generation. The prepared adsorbent can adsorb arsenic in real samples.
研究领域:
环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Journal of Aquatic Food Product Technology
·
2020
·
IF 1.02
Yan-Fei Liu,Zi-Xuan Wu,Jing Zhang,Yu-Xin Liu,Zhong-Yuan Liu,Hong-Kai Xie,Kanyasiri Rakariyatham,Da-Yong Zhou
Lipid profile of the oyster Crassostrea talienwhanensis from the Yellow Sea was evaluated based on the seasonal changes in lipid categories, phospholipid (PL) categories, fatty acid (FA) composition, atherogenicity index (IA), thrombogenicity index (IT), and glycerophospholipid (GP) molecular species. We observed that the samples harvested in February contained higher levels of polyunsaturated fatty acid (PUFA) (48.77% of total FAs) and PL (52.12% of total lipids) and had lower IA (0.29). In addition, more than 290 GP molecular species of glycerophosphocholine (GPCho), glycerophosphoethanolamine (GPEtn), glycerophosphoserine (GPSer), and glycerophosphoinositol (GPIns) were determined. The molecular species containing eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) constituted 75.24% of total GPCho in the samples harvested in February. Considering the lipid profile, oyster Crassostrea talienwhanensis harvested in February had better nutritional value.
研究领域:
营养学
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海洋生物学
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产品关联
DOI
高影响因子
Journal of Cleaner Production
·
2022
·
IF 11.072
Jing Li,Lu Cao,Bing Li,Haiming Huang,Wei Yu,Cairui Sun,Kehua Long,Brent Young
The activated sludge-based biochar modified by metals performs well for the phosphate adsorbent. The cost of the modification materials and the follow-up treatment of the spent adsorbents are significant limits for the large-scale application of the developed adsorbent. In this study, wasted egg shells and oyster shells, rich in calcium (Ca), were employed to load Ca onto the surface of the activated sludge-based biochar. The results revealed that different forms of Ca on egg shell-modified activated sludge biochar (ESB) and oyster shell modified activated sludge biochar (OSB) induced different adsorption performances. ESB had a greater phosphate adsorption capacity than OSB, where the maximum adsorption capacities were 154.18 mg/g and 129.03 mg/g, respectively. The saturated biochar could slowly release phosphate at different levels of pH; in fact, the phosphate release efficiency of ESB exceeded that of OSB. Economic analysis suggested that the cost of phosphate removal by ESB was $1.13 per kg, and by OSB was $1.22 per kg. The phosphate adsorption using the Ca-loaded biochar revealed good prospects for sustainable development with the advantages of the high efficiency of phosphate removal, low cost and resourceful employment of wastes.
研究领域:
环境科学
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废水处理
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DOI
高影响因子
AQUACULTURE
·
2021
·
IF 4.242
Shuang Li,Keyi Fang,Shubing Chen,Jilin Xu,Haimin Chen,Juanjuan Chen
The carotenoid profiling of Mytilus coruscus generally depends on the dietary microalgae, and the ingested carotenoids are modified and processed depending on the properties of different tissues. Although much research effort has focused on analyzing carotenoid profiling between microalgae and shellfish, the detailed metabolic pathways of dietary carotenoids in shellfish are poorly understood. In the present study, we carried out feeding experiments with different unialgal cultures ( Chaetoceros mulleri and Dicrateria inornata ) during the growth stages and examined the carotenoid profiles between the dietary microalgae and the corresponding tissues in order to study the resorption and metabolic transformations of dietary carotenoids in M. coruscus . The results showed that 1) carotenoids mainly occurred in the pre-spawning phase of M. coruscus; the contents of most carotenoids showed the same trends as in dietary microalgae. 2) An association analysis of the total content of carotenoids and relevant gene expression demonstrated that the main tissue for carotenoid synthesis in M. coruscus was the hepatopancreas . The synthetic reactions of the allenic to acetylenic carotenoids pathway and the 4-keto oxidative pathway in M. coruscus coexisted. 3) Quantitative analysis revealed that the carotenoids mytiloxanthin, alloxanthin, and pectenolone containing acetylene groups were selectively assimilated in gonad (69.3–74.1%), mantle (83.2–102%), and muscle (86.9–100%), forming a large percentage of the carotenoids present in those tissues. These results provide new insights into the biosynthetic and metabolic transformation pathways of carotenoids in M. coruscus .
研究领域:
海洋生物学
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生物化学
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产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
·
2023
·Vol. 125
·期 7
·2200161
·
IF 2.7
Ying Luo,Guan-Hua Zhao,Xue-Chen Pei,Fa-Wen Yin,Hui-Lin Liu,Lei Qin,Da-Yong Zhou
In this study, the antioxidant effects of gallic acid (GA) and its alkyl esters including methyl gallate (MG), butyl gallate (BG), octyl gallate (OG), and lauryl gallate (LG) in whole oyster meats and oyster meat homogenates during cold storage are investigated. The oxidation degree of lipid is measured by peroxide value (POV), thiobarbituric acid active substances (TBARS), and polyunsaturated fatty acid (PUFA) percentage. The results show that the lipid oxidation in the two types of samples is inhibited by GA alkyl esters, and the antioxidant effects first increase and then decrease along with the extension of the chain length, among the esters, BG and OG are relatively more effective. Therefore, it is speculated that the antioxidant capacity of GA alkyl esters may be influenced by hydrophobicity and follows a “cut-off effect” in whole oyster meats and oyster meat homogenates. Practical application : Seafood is easily oxidized even under cold storage because it contains more PUFA. Antioxidants are widely used to inhibit lipid oxidation. In this study, the antioxidant capacity of GA alkyl esters in whole oyster meats and oyster meat homogenates is investigated, which improves the selection of suitable antioxidants to strengthen the oxidation stability of oyster samples for cold storage.
研究领域:
食品科学
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海洋生物学
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 102
·期 4
·1651-1659
·
IF 3.639
Xiaohan Cong,Qi Wang,Cheng Sun,Fanqianhui Yu,Lipin Chen,Zhongkai Sun,Haohao Shi,Changhu Xue,Zhaojie Li
Background Oysters are mainly consumed in the raw form, so it is important to get rid of bacteria and other harmful substances. Ultraviolet (UV) sterilization depuration is a commonly used method and does not produce chemical residues or act directly on shellfish, resulting in minimal adverse effects on flavor. This study simulated the industrial depuration process using UV sterilization to depurate Pacific oysters ( Crassostrea gigas ). The effects of different temperatures (15, 20, and 25 °C) on the quality and taste components of C. gigas were investigated by measuring changes in physiological and biochemical indexes in C. gigas tissue samples. Results At the end of depuration, no oyster mortality occurred, but it was up to 55% at 25 °C at 84 h. Glycogen content decreased the most at 25 °C at 48 h. The fatty acid content was higher at 20 and 25 °C. Succinic acid content decreased significantly and was higher at 20 and 25 °C at 48 h with no significant difference. Total free amino acid (FAA) content was significantly higher at 20 °C, however, there were no significant differences in nucleotide content at any temperature at 48 h. Adenylate energy charge (AEC) values decreased, with higher values at 15 and 25 °C, and equivalent umami concentration (EUC) values increased, with higher values at 20 and 25 °C. Conclusion Considering the changes in flavor substances and mortality rate, 20 °C is the appropriate temperature for UV sterilization depuration of C. gigas to produce better edible quality. © 2021 Society of Chemical Industry.
研究领域:
水产养殖学
·
食品科学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Ying Luo,Na Li,Shuo Lv,Deyang Li,Yuanyuan Hu,Xiaoyang Liu,Dayong Zhou
The whole oyster and its three independent parts (adductor muscle, visceral mass and mantle) were processed into ready-to-eat (RTE) samples and stored at 40 °C for 8 weeks. During storage, the whole oyster samples showed liquid exudation and progressive darkening. In contrast, the color of the visceral mass sample, mantle sample and whole oyster sample deteriorated more obviously than that of the adductor muscle sample. This may be due to the higher contents of transition metal ions, lipids and reducing sugars in the formers, which promote lipid oxidation, Maillard/Maillard-like reaction, and phenolic oxidation - the main cause of color deterioration. The exuding liquid, containing browning reaction substrates such as lipids, sugars and polyphenols as well as browning reaction products, directly stains the adductor muscle while also indirectly accelerating its browning reaction, ultimately leading to uniform discoloration throughout the whole oyster sample.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Junbo Chu,Songyi Lin,Baoshang Fu,Xiangning Meng,Jiaxin Qiang,Simin Zhang
Fried oyster is a popular aquatic food product in East Asia, but nutrient loss during thermal processing become a significant concern. The goal of this research was to examine the impact of distinct frying techniques, including deep frying (DF), air frying (AF), and vacuum frying (VF), on the nutritional, textural and flavor characteristics of oysters. The VF method demonstrated superior retention of beneficial properties and flavor, and reduced protein and lipid oxidation compared to the DF and AF methods. Furthermore, proteomic analysis of oysters was attempted to explain the molecular mechanisms governing the influence of key differential proteins. 20 major differential proteins, including actin-2 protein, tryptophan 2,3-dioxygenase and 1-alph, involved in oyster protein oxidation were identified, annotated and analyzed to elucidate their influence mechanisms. This research provides a deeper understanding of intricate interactions between frying techniques and oyster biochemistry, which offers valuable implications for enhancing food quality in seafood industry
研究领域:
食品科学
·
蛋白质组学
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营养学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 15
·8081
·
IF 6.208
Xueqin Wang,Huahua Yu,Ronge Xing,Pengcheng Li
Alcohol-induced liver disease (ALD) has become one of the major global health problems, and the aim of this study was to investigate the characterization of the structure as well as the hepatoprotective effect and mechanism of oyster peptide (OP, MW < 3500 Da) on ALD in a mouse model. The results demonstrate that ethanol administration could increase the activities of aspartate aminotransferase (AST), alanine transaminase (ALT), γ-Glutamyl transferase (GGT), reactive oxygen species (ROS), malondialdehyde (MDA), and triglycerides (TG), as well as increase the interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor (TNF-α) levels (p< 0.01), and reduce the activity of superoxide dismutase (SOD) and the concentration of glutathione (GSH). Those changes were significantly reversed by the application of different doses of OP. Furthermore, the mRNA expressions of nuclear factor elythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and quinone oxidoreductase1 (NQO1) were significantly up-regulated in OP groups, and the mRNA expressions of nuclear factor kappa-light chain enhancer of B cells (NF-κB), TNF-α, and IL-6 were markedly reduced in OP groups compared to that of the model group. Thus, OP had a significant protective effect on ALD through the enhancement of the in vivo antioxidant ability and the inhibition of the inflammatory response as possible mechanisms of action, which therefore suggests that OP might be useful as a natural source to protect the liver from alcohol damage.Keywords:oyster peptide;alcohol-induced liver diseases;hepatoprotective effect;oxidative stress;inflammatory response
研究领域:
肝脏病学
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营养保健品
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分子生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）