Analytical Methods
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2014
·Vol. 6
·期 24
·9684-9690
·
IF 1.938
Xu Chen,Yong Luo,Bo Shi,Xianming Liu,Zhigang Gao,Yuguang Du,Weijie Zhao,Bingcheng Lin
β-Agonists are a group of illegal but widely used feed additives in the stockbreeding industry. In order to achieve simple-to-use, fast and high-throughput testing of this banned chemical, herein we suggest a paper-based analytical device on which a chemiluminescence diminishment method was performed. In this approach, extracts from swine hair samples as well as luminescent reagents, such as luminol and potassium periodate solution, in a low volume were applied to our device. It was found that the light emission was diminished by the β-agonists extracted from the swine hair samples. The degree of diminishment is proportional to the concentration of the β-agonists from 1.0 × 10−5 to 1.0 × 10−8 mol L−1. Also, the concentrations of solutions for chemiluminescence were optimized. The mechanism and reaction kinetics of chemiluminescence were discussed as well. The detection limit was obtained as 1.0 × 10−9 mol L−1, and recoveries from 96% to 110% were achieved, both of which suggested that our method will be favourable in field applications for swine hair samples.
研究领域:
分析化学
·
兽医学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 18
·2317-2322
·
IF 2.596
Wei Li,Yong Luo,Xiqing Yue,Junrui Wu,Rina Wu,Yu Qiao,Qing Peng,Bo Shi,Bingcheng Lin,Xu Chen
β-Agonists are illegal feed additives in the feed industries of many countries, especially China. Here, we report a microfluidic paper-based analytical device (μPAD) coupled with the chemiluminescence (CL) method to provide the sensitive, simple and rapid quantitative detection of β-agonists in swine hair samples. In this study, we found that the β-agonists diminished the CL generated by the reaction of K3[Fe(CN)6] and luminol on μPAD, which was different from that observed in the aqueous solution, and the degree of diminishment was proportional to the concentration of β-agonists. The possible mechanism was discussed as well. Also, this detection method showed a wide linear range (from 4.0 × 10−8 to 1.0 × 10−5 mol L−1) and low limit of detection (2.0 × 10−8 mol L−1) with a low consumption of samples and reagents. Satisfactory recovery values (from 78% to 95%) were achieved. Therefore, our μPAD CL sensor will be favorable to develop a miniaturized instrument for the on-site analysis of β-agonists in swine hair samples.
研究领域:
分析化学
·
食品安全
✓ 已验证
DOI
高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Dan Fei,Mengzhi Wang,Yujie Hou,Min Xie,Yaomin Zhou,Youhua Zhao,Lei Wu,Jun Xu
The indiscriminate use of organophosphorus pesticides poses a great threat to human health because of harmful substance bioaccumulation. We developed a rapid and reliable method for the simultaneous determination of chlorpyrifos, isocarbophos, methamidophos, omethoate, and triazophos in procine hair. The targets were separated by a C18 column (2.1 × 100 mm, 1.8 µm) and analyzed by electrospray ionization using the positive mode of a triple quadrupole mass spectrometer. The analytes had linear relationships from 1.0 to 100 µg/kg. The correlation coefficients were all greater than 0.998. The limits of detection ranged from 0.4 to 0.9 µg/kg, and the limits of quantification from 1.2 to 2.0 µg/kg. The recoveries were from 76.1% to 106.2%. The intra-assay and inter-assay precision rates were from 1.6% to 4.4% and 2.3% to 6.9%, respectively. The intra-day and inter-day accuracy ranged from 2.5% to 9.3% and 3.2% to 8.9%, respectively. This method was a valid tool to monitor organophosphorus pesticides in breeding and livestock products.
研究领域:
分析化学
·
环境科学
·
兽医学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Keyang Lai,Jun Xu,Kai Luo,Min Xie,Yuan Chen,Fan Li,Yaomin Zhou,Lihui Gong,Yonghua Xiong,Weihua Lai
Chloramphenicol (CAP) is a once widely used antibiotic, which is able to cause great harm to human health, is banned in some countries or organizations such as China, USA and the European Union for animal breeding. Because CAP in pig hair degraded slower and had amount of residues, pig hair could be used as the target to detect CAP residues. In this study, a competitive lateral flow immunoassay (LFIA), whose label was aggregation induced emission fluorescent nanobeads (AIEFN), was firstly developed for the detection of CAP in pig hair samples. It exhibited a low limit of detection (0.001 μg/kg) and a broad linear range (0.0025–0.32 μg/kg). The recovery rate and coefficient variation with spiked pig hair samples were 88.50%–106.17 % and 1.01%–7.37 %, which indicated good accuracy of this assay. The result of AIEFN-LFIA for the detection of CAP in pig hair was consistent with that of liquid chromatography-mass spectrometry. This assay was rapid with the total detection duration of about 20 min. AIEFN-LFIA was able to be used for rapid, sensitive, accurate and convenient detection of CAP residues in pig hair samples.
研究领域:
分析化学
·
兽医学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiaopeng Liu,Qiuyi Wang,Jie Wang,Lin Guo,Yanhai Chu,Changyang Ma,Wenyi Kang
A new polysaccharide (IHP-1aa) was isolated from the fruiting body of Inonotus hispidus by hot water extraction, ethanol precipitation and column chromatography . The molecular weight of IHP-1aa was 26.9 kDa. Structural analysis showed that IHP-1aa consisted of glucose (Glc), galactose (Gal), fucose (Fuc), mannose (Man) and contained a certain amount of 3- O -methylgalactose (3- O -Me-Gal). The structure was mainly composed of →6)- α / β -D-Glc p -(1→, →6)- α -D-Gal p -(1→, →6)-(3- O -Me)- α -D-Gal p -(1→, →6)- α- D-Man p -(1 → and →2, 6)- α -D-Gal p -(1 → as the main chain. Branched at O-2 with single β -L-Fuc p -(1 → 6)- α -D-Gal p -(1 → 6)- α -D-Glc p -(1 → as major the side chain. The results of SEM, XRD and AFM combined with Congo red indicated that IHP-1aa may be amorphous granular chain conformation. In addition, IHP-1aa stimulated macrophage function and improved phagocytic ability of RAW264.7, as well as promoted the secretion of NO , TNF-α and IL-6. IHP-1aa, a 3- O -methylgalactose-containing heteropolysaccharide, was isolated for the first time from the I. hispidus , which may be used as a potential immunomodulator in functional foods.
研究领域:
生物化学
·
免疫学
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药理学
·
真菌学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）