FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Shengqi Jiang,Huiyu Han,Madina Aitmagambetova,Aidana Rakhimova,Lin Li,Rong Bai,Chunjie Yang,Xiang Ye,Siying Wang,Jing Xi,Wu Ding
This study aimed to investigate the effects of oligosaccharides (xylooligosaccharides (XOS), fructooligosaccharides (FOS), galactooligosaccharides (GOS), and isomaltooligosaccharides (IOS)) with different structures and molecular weights on the glycosylation modification of pigskin collagen (CO). Glycosylated CO was prepared via the Maillard reaction, and its molecular structure, physicochemical, and functional properties were analyzed using various analytical techniques. Results indicated increased glycosylation significantly altered the α-helix and random coil content, intrinsic fluorescence intensity, particle size, and ζ-potential of CO ( P < 0.05). These modifications enhanced protein-protein and protein-water interactions, improving solubility, thermal stability, rheological properties, and creating a more uniform gel network structure. XOS, composed of the smaller molecular weight five-carbon aldose unit (xylose), exhibited the most significant effects, including enhanced thermal stability, water retention, and gel strength. However, the Maillard reaction-induced glycation browning caused a slight negative impact on the color of CO gels. In conclusion, oligosaccharide glycosylation significantly enhances the functional properties of pigskin CO, providing a promising strategy for its high-value utilization.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2016
·Vol. 182
·期 1
·55-66
·
IF 1.606
Fu Ruijie,Yao Kai,Zhang Qisheng,Jia Dongying,Zhao Jiayuan,Chi Yuanlong
A series of collagen hydrolysates (CHs) were prepared from pigskin shavings by using pepsin (PCH), trypsin (TCH), Alcalase (ACH), HCl (HCH), and NaOH (NCH). Their physicochemical properties, including degree of collagen hydrolysis, molecular weight distribution, electric charge, and microstructure, were investigated, and their flocculation performance was evaluated in a kaolin suspension, at varied pHs and concentrations. PCH exhibited high flocculation capability under acidic and neutral conditions, and its efficiency for removing suspended particles was approximately 80% at a concentration of 0.05 g/L. TCH, ACH, HCH, and NCH showed almost no flocculation capability. The flocculation capability of PCH could be mainly due to a combination of optimal molecular weight distribution and electric charge. This study could provide an environment-friendly natural flocculant and also proposes a promising approach for the reuse of collagen wastes.
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研究领域:
生物化学
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环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2023
·Vol. 15
·期 12
·2634
·
IF 5
Ngoc-Thang Nguyen,Tien-Hieu Vu,Van-Huan Bui
Leather is often used to make comfortable shoes due to its soft and breathable nature. However, its innate ability to retain moisture, oxygen and nutrients renders it a suitable medium for the adsorption, growth, and survival of potentially pathogenic microorganisms. Consequently, the intimate contact between the foot skin and the leather lining surface in shoes, which are subject to prolonged periods of sweating, may result in the transmission of pathogenic microorganisms and cause discomfort for the wearer. To address such issues, we modified pig leather with silver nanoparticles (AgPBL) that were bio-synthesized fromPiper betleL. leaf extract as an antimicrobial agent via the padding method. The evidence of AgPBL embedded into the leather matrix, leather surface morphology and element profile of AgPBL-modified leather samples (pLeAg) was investigated using colorimetry, SEM, EDX, AAS and FTIR analyses. The colorimetric data confirmed that the pLeAg samples changed to a more brown color with higher wet pickup and AgPBL concentration, owing to the higher quantity of AgPBL uptake onto the leather surfaces. The antibacterial and antifungal activities of the pLeAg samples were both qualitatively and quantitatively evaluated using AATCC TM90, AATCC TM30 and ISO 16187:2013 test methods, approving a good synergistic antimicrobial efficiency of the modified leather againstEscherichia coliandStaphylococcus aureusbacteria, a yeastCandida albicansand a moldAspergillus niger. Additionally, the antimicrobial treatments of pig leather did not negatively impact its physico-mechanical properties, including tear strength, abrasion resistance, flex resistance, water vapour permeability and absorption, water absorption and desorption. These findings affirmed that the AgPBL-modified leather met all the requirements of upper lining according to the standard ISO 20882:2007 for making hygienic shoes.Keywords:silver nanoparticles;green synthesis;Piper betleL. leaf;pig lining leather;antibacterial activity;antifungal activity
研究领域:
材料科学
·
生物学
·
皮革工程
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产品关联
DOI
PubMed
高影响因子
Acta Cirurgica Brasileira
·
2022
·
IF 1.564
Zhao Dan,Xiao Jinli,Qiang Lijuan,Deng Xingwang,An Jingjing,Zhang Qing,Zhao Fang,Ma Jiaxiang,Fang Chao,Guan Guangyu,Wu Yinsheng,Xie Yan
Purpose: To investigate the active ingredients of walnut ointment (WO) and its mechanism in repairing wounds. Methods: The ingredients of WO were detected by gas chromatography–mass spectrometry. The effect of linoleic acid (LA) was tested by in vitro Alamar Blue (AB) reagent. Image J software, histological and immunohistochemical analysis were used to confirm the healing effect of LA in the porcine skin model. The animals were euthanized after the experiment by injection of pentobarbital sodium. Results: LA, 24% in WO, promotes keratinocytes and fibroblasts proliferation, which were 50.09% and 15.07% respectively higher than control (p < 0.05). The healing rate of the LA group (96.02% ± 2%, 98.58% ± 0.78%) was higher than the saline group (82.11% ± 3.37%, 88.72% ± 1.73%) at week 3 and week 4 (p < 0.05). The epidermal thickness of the LA was 0.16 ± 0.04 mm greater and the expression of the P63 and CK10 proteins was stronger in the LA group than the control (p < 0.05). Conclusions: LA, which is the main components in WO can promote full-thickness burning wounds (FBWs) by stimulating cell proliferation and differentiation.
研究领域:
药理学
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伤口愈合
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皮肤病学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Sustainable Food Systems
·
2024
·
IF 3.7
Yang Ruoqing,Fei Zhuocheng,Wang Luyi,Tang Haohao,Sun Wanqiang,Li Mei,Lei Qingzi,Chen Jian,Guan Xin
Fibroblasts are important components of animal tissues such as muscle and skin, as they are the major producers of various matrix proteins. Matrix proteins such as collagen play an important role in meat products by providing unique nutrition, texture, and flavor. Cultured meat is an innovative meat alternative produced by culturing animal cells, but currently, relatively few studies have been conducted using fibroblasts as seed cells for cultured meat manufacturing. In this work, we first developed an innovative digestion-friction method for isolating fibroblasts from porcine skin efficiently and cost-effectively. After optimizing the enzymatic digestion and physical friction conditions, 2.39 ± 0.28 × 105 fibroblasts were obtained from 1 cm2 of porcine skin tissue, which was about 9 times higher than the conventional tissue explant method. In addition, we identified an edible bio-ink composed of gelatin and chitosan that has good printing properties and supports fibroblast adhesion and growth. Furthermore, we fabricated fibroblast-based cultured meat by 3D printing with an initial cell density of 1.0 × 107 mL−1 and evaluated its texture and nutritional properties. This work provides valuable insights and references for introducing fibroblasts into the production of cultured meat that is more comparable to structured animal meat.
研究领域:
组织工程
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生物打印
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细胞培养
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食品科学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Jiamin Xu,Lili Yang,Yinghua Nie,Mengyang Yang,Wenjuan Wu,Zhengquan Wang,Xichang Wang,Jian Zhong
Molecular modification has attracted many attentions in the field of gelatin science. In this work, a transglutaminase (TG) modification method was developed and applied to modify bovine bone gelatin, porcine skin gelatin, and cold-water fish skin gelatin, and then the effect of TG crosslinking on the structural, physicochemical, functional, and emulsion stabilization properties of three types of gelatins were explored and compared. The results demonstrated that TG modification increased the nanoparticle formation behaviors and change the physicochemical and functional properties of three types of gelatins. Further, TG-modified aquatic gelatin showed comparable foaming properties (147% ± 6% for foaming capability and 55% ± 4% for foam stability) and higher emulsifying properties (18 ± 1 m 2 /g for emulsion activity index and 142 ± 11 min for emulsion stability index) to unmodified mammalian gelatins. In addition, TG-modified gelatins decreased the droplet sizes and increased creaming stability of fish oil-loaded emulsions compared with unmodified gelatins. All these results suggested TG modification was an efficient molecular modification method for gelatins. Moreover, TG-modified aquatic gelatin could be potential mammalian gelatin replacer. This work could provide useful information to understand the relationship among animal sources, tissue sources, extraction methods, amino acid compositions, molecular modification, molecular structure, physicochemical properties, functional properties, and emulsification stability of gelatins.
研究领域:
食品科学
·
化学
·
生物技术
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Mengmeng Feng,Yize Li,Yanfei Jiang,Chunyue Zhao
There has been a growing interest in hydroxytyrosol (HT) due to its powerful antioxidant and free-radical scavenging properties when added to formulations such as pharmaceuticals and cosmetics. To study the stability and transdermal properties of hydrogels and creams (HT-based formulations), a high-performance liquid chromatography method was developed for determining HT. In the Franz diffusion cell system, both hydrogel and cream show a rapid and similar penetration profile through the Bama miniature pig skin. However, the Strat-M® membrane exhibits slightly lower permeability and is selective to different formulations; that is, the cream has a permeability value of 10.69%, while the hydrogel has a value of 5.27%. The dynamics parameters from the permeation assays indicate that the model using the Strat-M® membrane can be used as a screening tool to evaluate the skin uptake and permeation efficacy of different formulations. Adding 3- O -ethyl-L-ascorbic acid to HT-based formulations can effectively prevent discoloration under prolonged high-temperature storage, while combining multiple antioxidants delays degradation most effectively. This study provides novel ideas for functional formulation optimization to enhance the realism and reproducibility of cosmetic products containing HT and provides scientific evidence for the production, packaging, shelf life, storage, and transportation of products.
研究领域:
药学
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化妆品科学
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分析化学
·
制剂科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2019
·Vol. 102
·期 10
·5899-5909
·
IF 3.094
Puxian Xiong,Mingying Peng,Jiangkun Cao,Xueliang Li
Mechanoluminescence (ML) is a phenomenon upon external mechanical stimuli and it has found diverse applications such as stress sensing, structure health diagnosis, 3-D signature, energy harvesting etc because of its unique properties of in situ and real-time response to the stimuli. However, ML of most of the state-of-art phosphors primarily appears within the spectral range from ultraviolet to visible, which does not lie in the biological transparent windows, and, therefore, limits its applications in biological field. Here, we report a strong near infrared (NIR) ML from orthorhombic Cmcm perovskite Sr 3 Sn 2 O 7 : Nd 3+ , which is peaked at ~ 900 nm and located exactly within the first optical transparent window of tissues. The ML apparates after gradual discharge of the traps deeper than 0.73 eV triggered by the external mechanical stimuli and subsequently excitation of Nd 3+ to the state of 4 F 3/2 . The rechargable ML presents well repeatable linearity to loaded force at least up to 5000 N, and it can penetrate tissues easily that are thick up to 30 mm such as pigskin and the ceramic disk of hydroxy apatite which is the main constituent of bone. These results demonstrate the potential application for in situ biomechanical sensor. Meanwhile, by recording and processing these ML signals during signing on a pellet sample, for the first time, we provide a novel signature system based on NIR ML. This could raise the security level of existed signature anti-countering to a higher level.
研究领域:
材料科学
·
生物医学工程
·
光学
·
安全系统
✓ 已验证
DOI
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Wenjuan Wu,Cuiping Shi,Ye Zi,Huan Gong,Lijia Chen,Guangyi Kan,Xichang Wang,Jian Zhong
Herein, six types of polyphenol-crosslinked gelatin conjugates (PGCs) with ≥ two gelatin molecules were prepared using a covalent crosslinking method with two types of polyphenols (tannic acid and caffeic acid) and three types of gelatins (bovine bone gelatin, cold water fish skin gelatin, and porcine skin gelatin) for the emulsion stabilization. The structural and functional properties of the PGCs were dependent on both polyphenol and gelatin types. The storage stability of the conjugate-stabilized emulsions was dependent on the polyphenol crosslinking, NaCl addition, and heating pretreatment. In particular, NaCl addition promoted the liquid-gel transition of the emulsions: 0.2 mol/L > 0.1 mol/L > 0.0 mol/L. Moreover, NaCl addition also increased the creaming stability of the emulsions stabilized by PGCs except tannic acid-crosslinked bovine bone gelatin conjugate. All the results provided useful knowledge on the effects of molecular modification and physical processing on the properties of gelatins.
研究领域:
食品科学
·
胶体与表面化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Zhou Xiaosong,Guo Min,Wang Zongliang,Wang Yu,Zhang Peibiao
Bioactive microspheres coated with acellular matrix (aECM) have received extensive attention in bone tissue engineering. In this work, biomimetic microspheres with different aECM ratios, uniform size and controllable size were prepared easily by blending natural porcine dermal aECM and poly (lactic-co-glycolic acid) (PLGA) using electrohydrodynamic spraying and solidification actuated by solvent extraction (ESASE) method. In this work, the appropriate polymer concentration and preparation voltage were investigated, and the surface morphology of the microspheres were observed by scanning electron microscope. Sirius red was used to visualize aECM exposure on the surface of the microspheres. The in vitro and in vivo experiments was carried out to evaluate the bioactivity and osteogenic properties of the microspheres. The results showed that the morphology and size of PLGA microspheres had little influence on the aECM blending. In vitro experiments showed that the higher the content of aECM, the better the cell adhesion performance. In vivo, rat calvarial defect models were observed and characterized at 4 and 8 weeks postoperatively, and the values of BV/TV of 50aECM/PLGA were 47.57 ± 1.14% and 72.92 ± 2.19%, respectively. The results showed that the skull healing effect was better in aECM-containing microspheres. In conclusion, aECM/PLGA composite microspheres can increase cell adhesion performance through the addition of aECM. Moreover, in vivo experiments have proved that aECM/PLGA microspheres is beneficial to bone repair, which means the aECM/PLGA microspheres is a promising bone tissue engineering material.
研究领域:
组织工程
·
生物材料
·
生物医学工程
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Xiangping Wen,Lihong Shi,Guangming Wen,Yanyan Li,Chuan Dong,Jun Yang,Shaomin Shuang
We report a facile and eco-friendly strategy for the fabrication of highly fluorescent nitrogen-doped carbon nanodots (N-CDs) and demonstrate their applications for multicolor cellular imaging and Co 2+ sensing in living cells. One-pot hydrothermal method using pigskin as the precursor yields bright green-emitting N-CDs, providing an easy way for the production of N-CDs without a tedious synthetic methodology or the toxic/expensive solvents and starting materials. Doping N heteroatoms benefits the generation of N-CDs with stronger fluorescence emission. As-prepared N-CDs exhibit exceptional advantages including high fluorescent quantum yield (24.1%), excellent biocompatibility, low-toxicity, and satisfactory chemical stability. Depending on these superior properties, as-synthesized N-CDs have been successfully used for multicolor cellular imaging. More strikingly, as-synthesized N-CDs generates strong response to Co 2+ ions and gives rise to the fluorescence quenching and further application is extended to precise and quantitative detection of Co 2+ ions in living cells.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Ke Deng,Qin Huang,Xiaotong Yan,Yan Dai,Juan Zhao,Xia Xiong,Hailan Wang,Xianggui Chen,Pengfei Chen,Li Liu
Achieving the controllable detachment of polysaccharide-based wound dressings is challenging. In this study, a novel, photodetachable salecan-based hydrogel dressing with injectable, self-healing, antibacterial, and wound healing properties was developed using a green and facile approach. A salecan hydrogel with a uniform porous structure and water content of 90.4 % was prepared by simply mixing salecan and an Fe 3+ –citric acid complexing solution in an acidic D-(+)-glucono-1,5-lactone environment. Metal coordinate interactions were formed between the released Fe 3+ ions and carboxyl groups on the salecan polysaccharide, inducing homogeneous gelation. Benefiting from this dynamic and reversible crosslinking, the salecan hydrogel exhibited self-healing and injectable behavior, facilitating the formation of the desired shapes in situ. The exposure of Fe 3+ –citric acid to UV light (365 nm) resulted in the reduction of Fe 3+ to Fe 2+ through photochemical reactions, enabling phototriggered detachment. Moreover, the hydrogel exhibited excellent biocompatibility and satisfactory antibacterial efficacy against Escherichia coli and Staphylococcus aureus of 72.5 % and 85.3 %, respectively. The adhesive strength of the salecan hydrogel to porcine skin was 1.06 ± 0.12 kPa. In vivo wound healing experiments further highlighted the advantages of the prepared hydrogel in alleviating the degree of wound inflammation and promoting tissue regeneration within 12 days.
研究领域:
材料科学
·
生物材料
·
伤口愈合
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）