RSC Advances
·
2023
·Vol. 13
·期 51
·36098-36106
·
IF 3.9
Meng Cao,Feng Xu,Li Li,Honglian Zhang,Xin Liang,Tao Xu,Qian Li
The objective of this study was to establish a novel method for the detection of lean meat powder (salbutamol sulfate and terbutaline sulfate) residues in commercially available pig liver based on diazo coupling reaction by combining thin layer chromatography (TLC) with surface-enhanced resonance Raman scattering (SERRS). TLC was used to separate samples; after determining the spots of each component, diazo coupling reaction was carried out on the spots in situ to generate azo compounds, and then the spots of azo compounds mixed with silver sol on the TLC plate were qualitatively detected by SERRS. The limit of detection (LOD) of salbutamol sulfate was as low as 0.14 μg kg−1, and that of terbutaline sulfate was as low as 0.04 μg kg−1. The influence of the sample matrix in TLC-SERRS detection of salbutamol sulfate and terbutaline sulfate was investigated by experiment on a simulated positive sample, and salbutamol sulfate and terbutaline sulfate in commercially available pig liver were detected. Compared with the reported method (HPLC-MS/MS), this method has the advantages of strong specificity, high sensitivity, rapidity and low cost. It provides a new reference method for establishing and perfecting the safety system of veterinary drug residues.
研究领域:
食品安全
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分析化学
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2016
·
IF 1.303
Sovichea Lay,Hai-ning Yu,Bao-xiang Hu,Sheng-rong Shen
A pre-treatment methodology for clenbuterol hydrochloride (CLEN) isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers (MIPs). CLEN-imprinted polymers were synthesized by the combined use of ally-β-cyclodextrin (ally-β-CD) and methacrylic acid (MAA), allyl-β-CD and acrylonitrile (AN), and allyl-β-CD and methyl methacrylate (MMA) as the binary functional monomers. MAA-linked allyl-β-CD MIPs (M-MAA) were characterized by Fourier transform-infrared (FT-IR) spectroscopy and a scanning electron microscope (SEM). Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references: AN-linked allyl-β-CD MIPs (M-AN) and MMA-linked allyl-β-CD MIPs (M-MMA). M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction (MISPE) micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation (RSD) ≤4.45%.
研究领域:
分析化学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-11
·
IF 4.122
Xiao Qiling,Zhou Qiongqiong,Yang Lu,Tian Zhongyuan,Wang Xiliang,Xiao Yuncai,Shi Deshi
Human carboxylesterases has been proven to be age and race-related and a sound basis of clinical medication. PLE involve in signal transduction and highly catalyze hydrolysis. Therefore, the expression level of PLE most probably exist age and breed difference and lead to significant differences of pharmacology and physiology. Four age groups of Tongcheng (TC) and Large White (LW) pigs were selected to explore PLE breed and age differences, and it was found that PLE mRNA was most abundant in liver in both breeds. In liver, PLE levels and hydrolytic activities increased with age, and PLE levels (except for 3 month) and the hydrolytic activities were higher in LW than in TC across all age groups. Abundance of PLE isoenzymes was obvious different between breeds and among age groups. The most abundant PLE isoenzyme in LW and TC pigs was PLE-A1 (all age groups) and PLE-B9 (three early age groups) or PLE-G3 (adult groups), respectively. 103 new PLE isoenzymes were found, and 55 high-frequency PLE isoenzymes were accordingly classified into seven categories (A-G). The results of this research provide a necessary basis not only for clinical medication of pigs but also for pig breeding purposes.
研究领域:
兽医学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2017
·
IF 4.208
Liyun Ma,Xiaojing Mao,Xiao Sun,Li Xu
Non-steroidal anti-inflammatory drugs (NSAIDs) are one of the most frequently used pharmaceuticals in animals. In the current study, the biotransformation of five NSAIDs by pig liver microsomes (PLMs) was studied. The pseudo-first-order kinetics mode was obtained for the metabolization of the studied NSAIDs by PLMs in vitro . The metabolites were identified by high performance liquid chromatography with a high-resolution LTQ-Orbitrap mass spectrometry. The hydroxylation of benzene was confirmed to be the dominating metabolic pathway. Finally, the toxicity of the metabolites was predicted by the Estimation Programs Interface Suite software based on quantitative structure-activity relationships. Decreased toxicity was expected for the most metabolites of the studied NSAIDs except flurbiprofen, whose main metabolite exhibited slightly more toxicity. The present study provided a preliminary foundation to understand the metabolites of some NSAIDs and their toxicity, which was of great significance in animal food safety.
研究领域:
药理学
·
毒理学
·
动物医学
✓ 已验证
DOI
高影响因子
ACS Food Science & Technology
·
2022
·
IF 0
Miao Li,Jingjie Huang,Yingxian Chen,Yuejiao Ma,Sixuan Qian,Zhixuan Sun,Jianzhong Shen,Jiancheng Li
Ractopamine (RAC) is a feed additive that regulates protein synthesis. In this study, an indirect enzyme-linked immunosorbent assay (icELISA) for the detection of RAC residues in the porcine liver was developed based on our specific immunomagnetic bead (IMB) coated with ractopamine-single chain variable fragment (RAC-scFv). The intact RAC-scFv was constructed, and its key amino acid sites were analyzed. Finally, an icELISA based on immunomagnetic beads separation techniques (IMBs) of RAC from the porcine liver was established with an IC50 of 9.15 ng/mL and a linear range from 4.8 to 17.4 ng / mL. The IC50 of the prepared RAC-scFv was 8.7 ng/mL with a linear range of 3.8 to 19.6 ng/mL. The limit of detection (LOD) in the porcine liver of RAC was 4.57 μg/kg, and the recoveries ranged from 72.65 to 89.41% (CV < 15%).
研究领域:
分析化学
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兽医学
·
食品安全
✓ 已验证
DOI
JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 6
·1681-1688
·
IF 2.479
Guanqiong Na,Xiaofei Hu,Yaning Sun,Sharon Kwee,Guangxu Xing,Yunrui Xing,Gaiping Zhang
Valnemulin (VAL) and tiamulin (TIA) are pleuromutilin antibiotics used primarily for treating bacterial infections in swine or other food animals. Furthermore, VAL and TIA are also employed as feed additives to promote animal growth. However, the illegal use of VAL and TIA could cause a series of hazards to consumers. Here, VAL was designed to be conjugated with bovine serum protein to prepare immunogen. A highly sensitive monoclonal antibody that recognized both VAL and TIA has been successfully produced. Moreover, an immunochromatographic strip assay for rapidly screening VAL and TIA in porcine liver was established with visual detection limits (cutoff values) of 50 and 25 ng/g, respectively. The IC 50 values calculated from the equation of the standard curve were 6.06 and 3.45 ng/g and the limits of detection were 0.96 and 0.29 ng/g for VAL and TIA. According to the recovery experiment results, the test strip exhibited acceptable accuracy and precision. Generally, the proposed strip provided a practical tool for the detection of VAL and TIA. Practical Application We produced a highly sensitive monoclonal antibody and developed an immunoassay strip for simultaneously monitoring TIA and VAL. Additionally it was preliminarily confirmed that the rapid detection tool was suitable for screening TIA and VAL in porcine liver.
研究领域:
兽医学
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分析化学
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Zixu Wang,Lujuan Xing,Jiaming Cai,Fidel Toldrá,Yuejing Hao,Wangang Zhang
Alcoholic liver disease (ALD) is a worldwide disease caused by excessive ethanol consumption. In current study, hepatoprotective peptides were isolated from the porcine liver while their activities and interactions with ethanol metabolizing enzymes (alcohol dehydrogenase: ADH/acetaldehyde dehydrogenase: ALDH) were analyzed. The alcalase digested porcine liver hydrolysate (PLH) was confirmed to possess ADH/ALDH activation ability in a dose-dependent manner. The peptide sequences of PLH were identified by mass spectrometry. Molecular docking and in silico analysis were federatively used for screening. NTLPHPTAP had the lowest binding energy with ADH (−0.88 kcal/mol) and low toxicity. Fluorescence quenching data showed a dynamic fluorescence quenching constant (Kq) for NTLPHPTAP interacting with ADH/ALDH (ADH: 1.57 × 10 −10 M −1 s −1 ; ALDH:1.961 × 10 −10 M −1 s −1 ). Circular dichroism (CD) spectra and infrared spectrometer (FTIR) revealed that the enzyme secondary structures became flexible and disordered after interaction with NTLPHPTAP. Meanwhile, the ADH/ALDH was speculated to bind NTLPHPTAP via amido and carbonyl groups, respectively. These results indicated that the formation of ADH/ALDH-NTLPHPTAP complexes expanded the overall structure of the original enzymes and further increased the possibility of enzyme-substrate binding. In conclusion, the study proposed a method for the extraction of porcine liver hepatoprotective peptides and suggested further explanation for the interaction between NTLPHPTAP and ADH/ALDH in vitro .
研究领域:
生物化学
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药理学
·
分子生物学
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产品关联
DOI
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2019
·
IF 1.117
Leilei Zhao,Qiong Jiang,Sitong Luo,Jie Shen,Xinjun Xu
Hepatic stimulator substance (HSS) is prepared by the freeze–thaw method on a large scale, but it is time-consuming and inefficient. It is necessary to find a better method to improve the efficiency and yield of HSS. In this study, HSS was prepared by enzymatic hydrolysis of neonatal porcine liver with trypsin, papain, dispase, and alcalase. Relatively, dispase was found to be the best enzyme, based on the results of the degree of hydrolysis and MTT assay. Box–Behnken design-response surface method was used to optimize the conditions, which were as follows: enzyme (dispase) concentration, 4164 U/g; substrate concentration, 7% (w/v); hydrolysis time, 4.3 hr; temperature, 45 °C; and pH, 6.5. The degree of hydrolysis was (54.99 ± 1.57)%. Shotgun proteomics coupled with Gene Ontology (GO) analysis based on the PANTHER classification system was used to screen proteins from neonatal porcine liver. The results profiled the proteins into biological processes, molecular functions, and cellular components, thus laying a foundation for further studies on components involved in and mechanisms of liver regeneration. Overall, the results suggest that enzymatic hydrolysis might be a promising method for industrial application.
研究领域:
生物化学
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蛋白质组学
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生物技术
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Luo Shengchang,Wang Qingqing,Li Miaoting,Xu Peiyao,Wang Yicheng,Wang Ying,Kankala Ranjith Kumar,Wang Shibin,Chen Aizheng
The decellularized extracellular matrix (dECM) has emerged as an effective medium for replicating the in vivo-like conditions of the tumor microenvironment (TME), thus enhancing the screening accuracy of chemotherapeutic agents. However, recent dECM-based tumor models have exhibited challenges such as uncontrollable morphology and diminished cell viability, hindering the precise evaluation of chemotherapeutic efficacy. Herein, we utilized a tailor-made microfluidic approach to encapsulate dECM from porcine liver in highly poly(lactic-co-glycolic acid) (PLGA) porous microspheres (dECM-PLGA PMs) to engineer a three-dimensional (3D) tumor model. These dECM-PLGA PMs-based microtumors exhibited significant promotion of hepatoma carcinoma cells (HepG2) proliferation compared to PLGA PMs alone, since the infusion of extracellular matrix (ECM) microfibers and biomolecular constituents within the PMs. Proteomic analysis of the dECM further revealed the potential effects of these bioactive fragments embedded in the PMs. Notably, dECM-PLGA PMs-based microtissues effectively replicated the drug resistance traits of tumors, showing pronounced disparities in half-maximal inhibitory concentration (IC50) values, which could correspond with certain aspects of the TME. Collectively, these dECM-PLGA PMs substantially surmounted the prevalent challenges of unregulated microstructure and suboptimal cell viability in conventional 3D tumor models. They also offer a sustainable and scalable platform for drug testing, holding promise for future pharmaceutical evaluations.
研究领域:
生物医学工程
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药学
·
癌症研究
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2024
·
IF 5.5
Ziran Zhu,Huijun Ye,Kaiwen Zhang,Gang He,Zheng Pan,Yiwen Xian,Yu Yang,Chong Zhang,Decheng Wu
Developing biocompatible injectable hydrogels with high mechanical strength and rapid strong tissue adhesion for hemostatic sealing of uncontrolled bleeding remains a prevailing challenge. Herein, we engineer an injectable and photo-cross-linkable hydrogel based on naturally derived gelatin methacrylate (GelMA) and N-hydroxysuccinimide-modified poly(γ-glutamic acid) (γPGA-NHS). The chemically dual-cross-linked hydrogel rapidly forms after UV light irradiation and covalently bonds to the underlying tissue to provide robust adhesion. We demonstrate a significantly improved hemostatic efficacy of the hydrogel using various injury models in rats compared to the commercially available fibrin glue. Notably, the hydrogel can achieve hemostasis in porcine liver and spleen incision, and femoral artery puncture models. Moreover, the hydrogel is used for sutureless repair of the liver defect in a rat model with a significantly suppressed inflammatory response, enhanced angiogenesis, and superior healing efficacy compared to fibrin glue. Together, this study offers a promising bioadhesive for treating severe bleeding and facilitating wound repair.
研究领域:
生物医学工程
·
材料科学
·
止血
·
伤口愈合
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2024
·
IF 15.8
Yutao Ma,Zhihua Li,Yucheng Luo,Yao Chen,Le Ma,Xiaoya Liu,Jingyu Xiao,Man Huang,Yingnan Li,Hongliang Jiang,Meijuan Wang,Xiaoqian Wang,Jiangtao Li,Jian Kong,Peng Shi,Hanry Yu,Xingyu Jiang,Qiongyu Guo
Transarterial chemoembolization (TACE), the mainstay treatment of unresectable primary liver cancer that primarily employs nondegradable drug-loaded embolic agents to achieve synergistic vascular embolization and locoregional chemotherapy effects, suffers from an inferior drug burst behavior lacking long-term drug release controllability that severely limits the TACE efficacy. Here we developed gelatin-based drug-eluting microembolics grafted with nanosized poly(acrylic acid) serving as a biodegradable ion-exchange platform that leverages a counterion condensation effect to achieve high-efficiency electrostatic drug loading with electropositive drugs such as doxorubicin (i.e., drug loading capacity >34 mg/mL, encapsulation efficiency >98%, and loading time <10 min) and an enzymatic surface-erosion degradation pattern (∼2 months) to offer sustained locoregional pharmacokinetics with long-lasting deep-tumor retention capability for TACE treatment. The microembolics demonstrated facile microcatheter deliverability in a healthy porcine liver embolization model, superior tumor-killing capacity in a rabbit VX2 liver cancer embolization model, and stabilized extravascular drug penetration depth (>3 mm for 3 months) in a rabbit ear embolization model. Importantly, the microembolics finally exhibited vessel remodeling-induced permanent embolization with minimal inflammation responses after complete degradation. Such a biodegradable ion-exchange drug carrier provides an effective and versatile strategy for enhancing long-term therapeutic responses of various local chemotherapy treatments.
研究领域:
药学
·
生物医学工程
·
肿瘤学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）