Scientific Reports
·
2018
·Vol. 8
·期 1
·1-11
·
IF 4.122
Xiao Qiling,Zhou Qiongqiong,Yang Lu,Tian Zhongyuan,Wang Xiliang,Xiao Yuncai,Shi Deshi
Human carboxylesterases has been proven to be age and race-related and a sound basis of clinical medication. PLE involve in signal transduction and highly catalyze hydrolysis. Therefore, the expression level of PLE most probably exist age and breed difference and lead to significant differences of pharmacology and physiology. Four age groups of Tongcheng (TC) and Large White (LW) pigs were selected to explore PLE breed and age differences, and it was found that PLE mRNA was most abundant in liver in both breeds. In liver, PLE levels and hydrolytic activities increased with age, and PLE levels (except for 3 month) and the hydrolytic activities were higher in LW than in TC across all age groups. Abundance of PLE isoenzymes was obvious different between breeds and among age groups. The most abundant PLE isoenzyme in LW and TC pigs was PLE-A1 (all age groups) and PLE-B9 (three early age groups) or PLE-G3 (adult groups), respectively. 103 new PLE isoenzymes were found, and 55 high-frequency PLE isoenzymes were accordingly classified into seven categories (A-G). The results of this research provide a necessary basis not only for clinical medication of pigs but also for pig breeding purposes.
研究领域:
兽医学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yihua Wei,Weihong Li,Yan Han,Yan Xiong,Yuanying Kuang,Jinyan Zhang
Zilpaterol hydrochloride (zilpaterol) is used in animal feed as it can increase the lean meat mass. However, consuming zilpaterol-containing animal products may damage human health. Therefore, rapid detection of zilpaterol is attracting increasing research attention. This study aimed to developed a fast, accurate, and ultrasensitive fluorescence immunoassay based on CdTe quantum dots (QDs). A CdTe QD fluorescence sensor was synthesized from thioglycolic acid using a simple hydrothermal method. The morphology and structure of the CdTe QDs were characterized using transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy. The detection limits of our method in swine urine and pork samples were 0.5 μg/L and 1.2 μg/kg, respectively. A wide linear range of 0.1–10000 μg/L (R 2 = 0.996) was achieved. Both within-run precision (CVw) and between-run precision (CV b ) were ≤ 10 %. The method was then successfully applied for the analysis of zilpaterol contents in swine urine and pork samples.
研究领域:
分析化学
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食品安全
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Simin Xu,Wenxia Zheng,You Zeng,Xingguo Tian,Xiaoyan Xu
As a local pig breed, Mi pig (MP) is highly prized by Chinese consumers for its unique flavor and exceptional quality. This study aimed to investigate the differences in volatile profile between MP and commercial crossbred pigs (CP), as well as potential factors contributing to these variations. Results showed that MP had significantly higher intramuscular fat (IMF) content (7.75 %) and unsaturated fatty acid (UFA) content (56.44 %) compared to CP. Utilizing headspace solid-phase microextraction-gas chromatography tandem mass spectrometry (HS-SPME-GC–MS) and lipidomics, 14 volatile compounds and 310 lipids were identified as key factors influencing the flavor differences between the two breeds. Specifically, UFAs produced through glycerophospholipid and glycerolipid metabolic pathways play an essential role in developing the rich MP flavor. Our findings provide valuable insights into the underlying mechanisms responsible for the distinctive flavor of MP, highlighting the role of lipid metabolism in flavor formation.
研究领域:
食品科学
·
动物科学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2021
·Vol. 105
·期 11
·4751-4759
·
IF 4.813
Chen Dongjie,Wang Di,Wang Caixia,Wei Fang,Zhao Hongyuan,Lin Xiangmei,Wu Shaoqiang
Abstract Infection with African swine fever virus (ASFV) causes an acute and highly lethal hemorrhagic disease that has been responsible for huge economic losses in China. To exactly detect the antigen of ASFV, we established a rapid, no-wash, one-step sandwich-type immunoassay based on the amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA) using two monoclonal antibodies (mAbs) M-5 and M-6 against ASFV p72. ASFV p72 in samples was captured by biotinylated mAb M-5 connected to the donor bead surface via streptavidin and “sandwiched” by mAb M-6 which was coated onto the acceptor bead. Efficacy and sensitivity trials revealed that the AlphaLISA could detect ≥0.78 ng/ml of purified p72 and with a linear range of 0.78–100 ng/ml. The AlphaLISA was specific for ASFV and did not cross-react with other common pathogenic porcine viruses. Compared with RealPCR ASFV DNA test and ASFV antigen detection kit, the sensitivity of the AlphaLISA evaluated in 60 porcine serum samples was 93% and 100%, respectively. The specificity was 100% and 91.7%, respectively. This study presents a good laboratory diagnostic tool for sensitive and efficient detection of ASFV in porcine serum. Key points • MAbs M-5 and M-6 recognized various epitopes of ASFV p72. • The established ASFV p72 AlphaLISA showed well specificity, high sensitivity, and satisfied correlation coefficient.
研究领域:
兽医学
·
分子诊断学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
·
2018
·Vol. 41
·期 5
·706-718
·
IF 1.441
L. Zhang,Y. Li,Y. Wang,A. Sajid,S. Ahmed,X. Li
The aims of this study were to establish optimal doses of doxycycline (dox) against Haemophilus parasuis on the basis of pharmacokinetic–pharmacodynamic (PK-PD) integration modeling. The infected model was established by intranasal inoculation of organism in pigs and confirmed by clinical signs, blood biochemistry, and microscopic examinations. The recommended dose (20 mg/kg b.w.) was administered in pigs through intramuscular routes for PK studies. The area under the concentration 0- to 24-hr curve (AUC 0–24 ), elimination half-life ( T ½ke ), and mean residence time (MRT) of dox in healthy and H. parasuis -infected pigs were 55.51 ± 5.72 versus 57.10 ± 4.89 μg·hr/ml, 8.28 ± 0.91 versus 9.80 ± 2.38 hr, and 8.43 ± 0.27 versus 8.79 ± 0.18 hr, respectively. The minimal inhibitory concentration (MIC) of dox against 40 H. parasuis isolates was conducted through broth microdilution method, the corresponding MIC 50 and MIC 90 were 0.25 and 1 μg/ml, respectively. The Ex vivo growth inhibition data suggested that dox exhibited a concentration-dependent killing mechanism. Based on the observed AUC 24 hr /MIC values by modeling PK-PD data in H. parasuis -infected pigs, the doses predicted to obtain bacteriostatic, bactericidal, and elimination effects for H. parasuis over 24 hr were 5.25, 8.55, and 10.37 mg/kg for the 50% target attainment rate (TAR), and 7.26, 13.82, and 18.17 mg/kg for 90% TAR, respectively. This study provided a more optimized alternative for clinical use and demonstrated that the dosage 20 mg/kg of dox by intramuscular administration could have an effective bactericidal activity against H. parasuis .
研究领域:
兽医学
·
微生物学
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药理学
✓ 已验证
DOI
高影响因子
JOURNAL OF CELLULAR PHYSIOLOGY
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2018
·Vol. 233
·期 12
·9763-9776
·
IF 3.923
Xing Xie,Yuan Gan,Maoda Pang,Guoqing Shao,Lei Zhang,Beibei Liu,Qi Xu,Haiyan Wang,Yanyan Feng,Yanfei Yu,Rong Chen,Meng Wu,Zhenzhen Zhang,Lizhong Hua,Qiyan Xiong,Maojun Liu,Zhixin Feng
Primary porcine bronchial epithelial cells (PBECs) are an ideal model to study the molecular and pathogenic mechanisms of various porcine respiratory pathogens. However, the short lifespan of primary PBECs greatly limit their application. Here, we isolated and cultured primary PBECs and established immortalized PBECs by transfecting primary PBECs with the pEGFP-hTERT recombinant plasmid containing human telomerase reverse transcriptase (hTERT). Immortalized PBECs (hTERT-PBECs) retained the morphological and functional features of primary PBECs as indicated by cytokeratin 18 expression, telomerase activity assay, proliferation assays, karyotype analysis, and quantitative reverse-transcriptase polymerase chain reaction. Compared to primary PBECs, hTERT-PBECs had higher telomerase activity, extended replicative lifespan, and displayed enhanced proliferative activity. Moreover, this cell line is not transformed in vitro and does not exhibit a malignant phenotype in vivo, suggesting that it can be safely used in further studies. Besides, hTERT-PBECs were susceptible to swine influenza virus of H3N2 subtype and porcine circovirus type 2. In conclusion, the immortalized hTERT-PBECs represent a valuable in vitro model, which can be widely used in the study of porcine respiratory pathogenic infections.
研究领域:
细胞生物学
·
病毒学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2023
·Vol. 170
·期 4
·047505
·
IF 3.9
Yao Xiong,Ruimei Wu,Lulu Xu,Ying Zhong,Yu Ge,Yangping Wen,Hang Yao,Weiqi Zhou,Shirong Ai
Extraordinary electronic performance and unique structural characteristic of black phosphorene (BP) often is used as electrode modified materials in electrochemical sensors. In this paper, a machine learning (ML) strategy for phosphorene nanozyme sensor and its the intelligent of clenbuterol (CLB) in pork and pig serum samples is prepared. The silver nanoparticles decorate BP to prevent oxidative degradation of BP surface and further hybridize with multi-walled carbon nanotubes (MWCNTs) composites containing nafion (Nf) treated with isopropanol (IP) to improve environmental stability and electrocatalytic capacity of BP. Back-propagation artificial neural network (BP-ANN) model combined with genetic algorithm (GA) is employed to optimize sensor parameters such as BP concentrations, MWCNTs concentrations and ratio of VNf:VIP, and compared with orthogonal experimental design (OED). Least square support vector machine, radial basis function and extreme learning machine are implemented to establish quantitative analysis model for CLB. The results showed that the CLB response current of BP sensor by BP-ANN-GA was improved 9.02% over OED method. Compared with the traditional linear regression, three models displayed better predictive performance, and LS-SVM was the best with the R2, RMSE and MAE and RPD of 0.9977, 0.0303, 0.0225, and 18.74, respectively. The average recoveries of CLB in pork and pig serum was 98.66% ∼ 101.67%, and its relative standard deviations was 0.19% ∼ 0.84%, indicating that electrochemical sensor using machine learning for intelligent analysis of CLB in animal-derived agro-products products was both feasible and practical.
研究领域:
分析化学
·
食品科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Jiahao Li,Yun Bai,Fang Li,Yue Zhang,Qingyun Xie,Lei Zhang,Lizhong Hua,Qiyan Xiong,Yanke Shan,Zhigao Bu,Guoqing Shao,Zhixin Feng,Dongming Zhao,Fei Liu
African swine fever (ASF) is a swine viral disease that could cause highly contagious and extremely high mortality, causing huge economic losses to the pig industry. As there is currently no vaccine and effective treatment methods. Therefore, early monitoring is one of the most important solutions to prevent and control ASF. In this study, the dual QDM recombinant virus protein 30 and 54 (P30 and P54) probes and pre-incubation in vitro were proposed for the first time as QDM based-ASFV immunosensor (QAIS) for the ultra-sensitive quantitative detection of ASFV antibodies in serum. In the range from serum dilution of 1:1000 to 1:64000, it showed a good linear relationship (R 2 = 0.9947), and its detection sensitivity was 1:64000 dilution. Compared with commercial enzyme-linked immunosorbent assay (ELISA) and colloidal gold immunochromatographic strip (CGICS), its detection sensitivity was improved by at least one order of magnitude and four orders of magnitude respectively. In addition, the whole ASFV antibody screening test can be completed in 25 min with simple operation. The performance and practicability of the established QAIS sensor have been verified by ASF-ELISA kit, and its coincidence rate was as high as 98.7% in 151 clinical samples. We firmly believe that the proposed QAIS sensor could potentially be applied to point-of-care testing (POCT) for quantitative ASFV antibody in pig farms.
研究领域:
兽医学
·
免疫学
·
诊断技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Zhaoyuan He,Xiaoge Duan,Zhi Zhao,Yingkai Chen,Chengxiang Fu,Fuxin Zhang,Jinzi Wang,Jianyuan Feng,Nanxin Lin,Hailan Chen
Porcine epidemic diarrhea virus (PEDV) and porcine rotavirus (PoRV) are the two main pathogens causing porcine diarrhea, which are characterized by high morbidity and mortality. Most of the diagnostic methods available are limited to the laboratory or fail to highlight their advantages in terms of target species, detection time, sensitivity, and stability. To meet the demand for rapid on-site diagnosis of PEDV and PoRV co-infection, a surface-enhanced Raman scattering (SERS) immunochromatographic sensor based on gold magnetic nanoparticles (MNPs) was developed. The sensor is dual-mode, detecting on the basis of color signals by the naked eye and Raman signals. After a series of optimizations, the constructed sensor could perform simultaneous qualitative and quantitative detection of PEDV and PoRV in just 18 min, with visualized (color signals observed by the naked eye) limits of detection (LOD) of 3.13 × 10 2 TCID 50 /mL and 4.69 × 10 2 copies/μL, respectively. The LOD based on Raman signal analysis was as low as 4.63 × 10 1 TCID 50 /mL and 3.30 × 10 2 copies/μL for PEDV and PoRV, respectively. In addition, the sensor exhibited excellent specificity without cross-reactivity with common pathogens. The overall compliance rate with RT-PCR was 92.1 % (35/38) for 38 clinical samples. Therefore, the sensor is characterized by high sensitivity, specificity, reproducibility, and accuracy, making it suitable for the simultaneous rapid on-site detection of PEDV and PoRV.
研究领域:
兽医学
·
诊断学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 31
·17595-17605
·
IF 2.936
Jing He,Guanghong Zhou,Yun Bai,Chao Wang,Shuran Zhu,Xinglian Xu,Chunbao Li
We investigated the effects of different pork preparation methods (cooked pork, emulsion-type sausage, dry cured pork, and stewed pork) on protein structures and in vitro digestion. Compared with raw meat, processed meats contained lower levels of free sulfhydryl groups (P < 0.05). Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed different protein profiles for pork products treated without or with 5% βME, which indicated different extents of disulfide bond formation. Emulsion-type sausage showed significantly higher α-helix content and lower β-sheet, β-turn, and random coil contents than cooked pork (P < 0.05). Correspondingly, emulsion-type sausage and dry-cured pork had the highest values of surface hydrophobicity (P < 0.05). Proteome data showed that the long salting and drying times used for dry-cured pork as well as long-term high-temperature cooking of stewed pork might alter the accessibility of digestive proteolytic enzymes to the protein cleavage sites.
研究领域:
食品科学
·
蛋白质化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENERGY & FUELS
·
2024
·
IF 5.2
Yuxiang Chen,Chao Li,Lijun Zhang,Shu Zhang,Jun Xiang,Song Hu,Yi Wang,Mortaza Gholizadeh,Xun Hu
Pig manure is a typical solid waste, which features a high content of inorganics. These inorganics might be involved in thermochemical conversion of pig manure, and this was investigated herein by pyrolysis of pig manure leached with varied concentrations of H2SO4 (0.5–6 mol L–1) at 600 °C. The removal of acid-leachable inorganics [CaCO3, Ca3(PO4)2, alkaline oxides, etc.] exposed more acidic sites derived from the oxides, like SiO2. This enhanced production of the gases at the expense of bio-oil. Furthermore, aromatization and secondary condensation reactions were also promoted, increasing not only the yield of char (from 29.6 to 39.7%, on an organic basis) but also the carbon content in char from 16.1% (blank sample) to 26.2% (leached with 6 mol L–1 H2SO4). Leaching partial inorganics rendered more sufficient contact and interaction between the organics, facilitating charring of organic matter. This further retained more carbon skeleton for generation of more micropores, which further enhanced the capability of the resulting char for adsorption of phenol. In situ infrared analysis indicated the presence of more abundant oxygen-containing functionalities and carbonaceous species after the leaching treatment, favoring the occurrence of aromatization reactions.
研究领域:
环境科学
·
能源转化
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 51
·36098-36106
·
IF 3.9
Meng Cao,Feng Xu,Li Li,Honglian Zhang,Xin Liang,Tao Xu,Qian Li
The objective of this study was to establish a novel method for the detection of lean meat powder (salbutamol sulfate and terbutaline sulfate) residues in commercially available pig liver based on diazo coupling reaction by combining thin layer chromatography (TLC) with surface-enhanced resonance Raman scattering (SERRS). TLC was used to separate samples; after determining the spots of each component, diazo coupling reaction was carried out on the spots in situ to generate azo compounds, and then the spots of azo compounds mixed with silver sol on the TLC plate were qualitatively detected by SERRS. The limit of detection (LOD) of salbutamol sulfate was as low as 0.14 μg kg−1, and that of terbutaline sulfate was as low as 0.04 μg kg−1. The influence of the sample matrix in TLC-SERRS detection of salbutamol sulfate and terbutaline sulfate was investigated by experiment on a simulated positive sample, and salbutamol sulfate and terbutaline sulfate in commercially available pig liver were detected. Compared with the reported method (HPLC-MS/MS), this method has the advantages of strong specificity, high sensitivity, rapidity and low cost. It provides a new reference method for establishing and perfecting the safety system of veterinary drug residues.
研究领域:
食品安全
·
分析化学
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）