FOOD CHEMISTRY
·
2021
·
IF 7.514
Chuanli Hou,Liangyun Liu,Jiaoyan Ren,Min Huang,Erdong Yuan
Hericium erinaceus , a traditional edible mushroom, is known as a medicine food homology to ameliorate gastrointestinal diseases. However, the relationship between the structural characteristics of Hericium erinaceus and its stomach-protecting activity remains unclear. Here, the structural properties of two polysaccharides from Hericium erinaceus, mycelium polysaccharide (HMP) and fruiting body polysaccharide (HFP) were investigated by spectral approaches. The results showed that the distribution of HMP was more uniform compared to HFP. Both HMP and HFP have triple helix structures, but the HMP conformation showed greater stability. Subsequently, the preventive effect of HMP and HFP on ethanol-induced gastric mucosal injury was also evaluated in rats and GES-1 cells, and it showed that both HMP and HFP had significant protective activity against gastric mucosal injury, but HMP showed better activity than HFP. These results suggested that conformational stability polysaccharide in Hericium erinaceus is more related to its gastric-protecting activity.
研究领域:
食品科学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Pin Gong,Shan Yue,Jie Wang,Ke Xu,Wenjuan Yang,Nan Li,Jing Wang,Yanni Zhao,Fuxin Chen,Yuxi Guo
This study investigates the effects of ultrasound synergistic pH shift modification on the structural and functional properties of Hericium erinaceus (HE) proteins. The modification resulted in significant changes in the molecular structure of HE proteins, including increased solubility (49.69 % at pH 1.5 and 61.30 % at pH 12.5), enhanced surface hydrophobicity (from 721.00 to 3377.00), and the exposure of free sulfhydryl groups, which rose from 9.3 μmol/g in the control to 19.9 μmol/g at pH 12.5. The modification also led to a reduction in particle size, improving oil-holding capacity and foaming properties, with foaming stability increasing from 10.5 % to 60.3 % at pH 12.5. Furthermore, the emulsification activity was significantly enhanced (187.5 % at pH 12.5). Rheological analysis revealed that ultrasound-modified proteins exhibited improved flow stress and gelation properties, with a shear recovery rate of 65.71 %. In 3D printing applications, the modified HE proteins demonstrated better printability, structural stability, and mechanical integrity, attributed to the enhanced molecular interaction and gelation properties. These findings suggest that ultrasound-assisted pH shift modification effectively alters the structure and functionality of HE proteins, making them suitable for use as a versatile raw material in food applications, particularly in 3D food printing.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2019
·
IF 5.839
Ming Cai,Si Chen,Qinghua Ma,Kai Yang,Peilong Sun
In this study, isolation of crude oligosaccharides from Hericium erinaceus (CHOS), and its physicochemical properties and proliferative effects on lactic acid bacteria were investigated. CHOS was obtained by an integrated membrane technology with microfiltration (MF, PVDF 0.1 μm), ultrafiltration (UF, PES 1000 Da) and nanofiltration (NF, PA 300 Da) membranes as the retentate of NF. During MF, UF and NF processes, compositions of total sugars, total reducing sugars, total proteins, total polyphenols and uronic acid changed significantly. CHOS accounts for 14.84% of total polysaccharides and its purity was 63.71%. Other compounds in CHOS, including reducing sugar, protein, polyphenol, uronic acid and others were 8.06%, 4.83%, 5.65%, 6.45% and 11.30%, respectively. CHOS has a good proliferation effect on lactobacillus , which the best concentration accounts for 25% of the carbon source.
研究领域:
食品科学
·
微生物学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Jing-Yu Liu,Xiao-Xiao Hou,Zhuo-Yu Li,Shu-Hua Shan,Ming-Chang Chang,Cui-Ping Feng,Yin Wei
The fruiting body of Hericium erinaceus has been used to treat digestive system disorder-related diseases for over 2000 years in China. A novel polysaccharide, HEFP-2b, was obtained from H. erinaceus fruiting bodies. Physical and chemical analysis showed that HEFP-2b consisted of fucose, galactose, glucose, and mannose in molar ratio of 11.81:22.82:44.28:21.09, and that its molecular weight was 3.252 × 10 4 Da. The backbone of HEFP-2b consisted of →6)-linked-α-D-Glc p -(1→ and →4)-β-D-Gal p -(1→ and →3,6) -α-D-Man p linkage, with two side-branching units of (1→ and →6)-β-D-Gal p and (1→ and →4)-α-D-Man p , terminated by Glc and Fuc. The results of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell cycle arrest experiments revealed that HEFP-2b considerably inhibited the growth of colon cancer cells (HCT-116) in vitro . The growth inhibitory effects of HEFP-2b correlated with their ability to arrest the cell cycle at the S -phase. Our results will provide valuable information for future studies on HEFP-2b as a novel health-promoting functional food ingredient that can be used for treating colon cancer.
研究领域:
药理学
·
肿瘤学
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生物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Yaowen Liu,Yue Wang,Siwei Shen,Md Alomgir Hossen,Dur E. Sameen,Saeed Ahmed,Jianwu Dai,Siyu Xiao,Suqing Li,Wen Qin
In order to increase the stability and the release time of nisin, nisin/ Tremella fuciformis polysaccharide nanoparticles (NTNs) were prepared and evaluated based on their uniform particle size dispersion (168–356 nm) and high loading rate (70–86%). The condensation reaction between 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and hydroxysuccinimide (EDC/NHS) amide was successfully used to prepare novel live particles (NTN@LP). When the mass ratio of NTN and LP was 2:1, the bacterial viability (85.6 ± 2.11%) and grafting efficiency (68.8 ± 1.96%) of the NTN@LP were both relatively high. The hemolytic activity and in vitro cytotoxicity indicated that NTN@LP is safe to use in food. The NTN@LPs were then used as natural microbial preservatives in sausages. The pH value, water activity, thiobarbituric acid-reactive substances value, and sensory evaluation of the sausages were used to assess the effect of NTN@LPs on the quality of the sausages during fermentation. NTN@LPs played an important role in reducing and decomposing biogenic amines (BAs) throughout the sausage fermentation process and displayed a strong inhibitory effect on histamine (>95%). Compared to that of the control group, the total amount of BAs in the 2:1 group was reduced by 67.90%. Tyramine content in the 2:1 NTN@LP group decreased by 78.16% compared to that in the control group, while tyramine content in the pure LP group was decreased by only 38.25%. These results indicate that NTN@LP can be potentially applied as a natural microbial preservative in the food industry.
研究领域:
食品科学
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微生物学
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生物化学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Changrong Wang,Mengfan Lin,Yibin Li,Weijing Zhuang,Zebin Guo
Steam explosion (SE) technology is an effective modification method for improving resource utilization of edible fungi processing by-products. In this study, the effect of SE-modified Tremella fuciformis ( T. fuciformis ) stem soluble dietary fiber (SDF) on the quality and digestibility of biscuits was investigated. The results showed that the addition of SE-modified T. fuciformis stem SDF (M-SDF) changed the gluten network structure and moisture distribution in the biscuits, which improved the spread ratio of the biscuits and resulted in attractive colors. Meanwhile, as starch was embedded, the starch hydrolysis rate (from 60.9 ± 0.90 % to 43.01 ± 0.78 %) and estimated glycemic index (from 84.10 ± 4.39 to 68.45 ± 3.15) of 12 % M-SDF biscuits were reduced. Furthermore, 8 % M-SDF received the highest sensory scores. These results demonstrate the potential applicability of SE-modified edible fungi processing by-product SDF as an additive in functional foods.
研究领域:
食品科学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2019
·
IF 5.839
Tao Feng,Mengzhu Shui,Zhongqiu Chen,Haining Zhuang,Wenxin Wang,Yan Yang,Jingsong Zhang,Ran Ye
The mechanism of Hericium Erinaceus β-glucan inhibiting starch digestion has been seldom studied. In this study, the mechanism of inhibited starch digestion was explored in the context of interaction between β-glucans of different molecular weights (Mw) and starch. The rates of starch digestion and glucose release decreased with increased Hericium erinaceus β-glucan ( HEBG ) Mw. HEBG reduced leached amylose but increased peak, minimum and final viscosity. In laser confocal scanning microscopy experiments, HEBG was observed to coat the starch. The mechanisms of HEBG modulating glucose release and starch digestion might be due to increase system viscosity and coat starch. We address the specific mechanisms by which starch granules interact with β-glucan molecules and thus contribute to further applications to develop starchy foods with low predicted Glycemic Index (pGI).
研究领域:
食品科学
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营养学
✓ 已验证
产品关联
DOI
高影响因子
Natural Product Communications
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2015
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IF 0.906
Caihui Wang,Wen Su,Xingli Su,Guojun Ni,Tao Liu,Yi Kong
The gastric mucosa protection effect of three natural plant extracts, Hericium erinaceus (HE), Centella asiatica (CA) and Amomum villosum (AV), were evaluated using the indomethacin damage model. Compared with a single extract, a combination of HE/CA/AV, especially with the ratios of 80:10:10, 45:45:10 and 45:10:45, showed significant synergistic effects for protection of the gastric mucosa with gastric ulcer inhibition rates of 97.8 ± 0.7%, 86.5 ± 2.8% and 86.1 ± 3.6%, respectively. Microscopic appearances of the gastric mucosa were carried out to help confirm the results.
研究领域:
药理学
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胃肠病学
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天然产物化学
✓ 已验证
DOI
高影响因子
Food & Function
·
2020
·Vol. 11
·期 7
·6128-6138
·
IF 4.171
Xiao-xiao Hou,Jing-yu Liu,Zhuo-yu Li,Ming-chang Chang,Min Guo,Cui-ping Feng,Jiang-ying Shi
The fruiting bodies of Hericium erinaceus (Bull.) Pers. are commonly used in China in the treatment of digestive system diseases. In this work, the polysaccharides from the fruiting bodies of Hericium erinaceus (HEFPs) were extracted, and their effects on human colorectal cancer cells (HCT-116 and DLD1) were investigated in vitro. Our results showed that HEFPs were mainly composed of arabinose, galactose, glucose, and mannose at a molar ratio of 8.99 : 11.15 : 1.2 : 1.97. They significantly inhibited the growth of these cells by inducing apoptosis by the modulation of Bax and Bcl-2 expression, which in turn induced the loss of mitochondrial membrane potential, leading to the activation of cleaved-caspase-9 and cleaved-caspase-3. These results suggested that HEFPs induced apoptosis via the caspase-9-depedent intrinsic mitochondrial pathway. Furthermore, HEFPs increased the level of reactive oxygen species (ROS) in HCT-116 and DLD1 cells. The addition of the antioxidant N-acetyl-L-cysteine reduced the ability of HEFPs to trigger the intrinsic mitochondrial pathway, indicating the role of ROS generation in the upstream pathway of HEFP-induced apoptosis. Therefore, the results described in this study could be of interest for further studies in finding functional foods or alternative therapeutic agents against colorectal cancer.
研究领域:
肿瘤学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Jing Liao,Huihua Huang
In this work, a low-cost and sustainable non-animal chitin from Hericium erinaceus residue was used to prepare hydrogels through a facile procedure. The chitin was firstly dissolved in NaOH/Urea aqueous solution by freeze thawing , and then the obtained chitin solution was directly regenerated to form hydrogels. The results showed that the extracted chitin could dissolve in NaOH/urea solvent system. The chitin solutions with concentrations from 1% to 4% showed no gelation behavior, while those with concentrations from 5% to 7% could form hydrogels. The obtained hydrogels showed elastic characteristic and desirable transmittance. In addition, the hydrogels with higher chitin concentration exhibited more compact gel network and improved gel strength, but decreased swelling degree and cumulative drug release. Compared with neat chitin, the thermal stability of the regenerated chitin hydrogels was also improved. The 5-Fu could be gradually released from the hydrogels, which followed Fickian diffusion model.
研究领域:
材料科学
·
生物技术
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Panling Yu,Xueyu Pan,Mingjie Chen,Jianshuai Ma,Baoting Xu,Yan Zhao
Dietary fiber (DF) is an important active polysaccharide in Hericium erinaceus . Obesity can lead to a wide range of diseases. In this work, we investigated the in vitro lipid-lowering effect of soluble dietary fiber (SDF) from H. erinaceus , aiming to provide a basis for the subsequent development of lipid-lowering products. Ultrasound-assisted enzymatic extraction (UAEE) of SDF from H. erinaceus was performed. The optimal extraction parameters determined via single-factor experiments and response surface methodology (RSM) were as follows: Lywallzyme concentration, 1.0%; complex protease concentration, 1.2%; ultrasonication time, 35 min; and ultrasonication power, 150 W. In vitro lipid-lowering experiments revealed that the adsorption amount of cholesterol micelles by H. erinaceus SDF was 11.91 mg/g. The binding amount and binding rate of sodium taurocholate were 3.73 mg/g and 42.47%, respectively, and those of sodium glycocholate were 3.43 mg/g and 39.12%, respectively. The pancreatic lipase inhibition rate reached 52.11%, and the type of inhibition was competitive. Therefore, H. erinaceus SDF has good in vitro lipid-lowering ability.
研究领域:
食品科学
·
营养学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Current Research in Food Science
·
2024
·
IF 6.2
Yufei Zhang,Yanyin Lu,Dandan Pan,Yanyan Zhang,Chen Zhang,Zexin Lin
Despite lignocellulose hindering the extraction of intracellular components, tea residue can serve as an excellent substrate for fungal fermentation owing to their lignocellulose-degrading abilities. Thus, the fermentation efficiencies of Lentinus edodes , Lentinus sajor-caju (Fr.) , Flammulina filiformis , Hericium erinaceus , Pleurotus pulmonarius , and Monascus kaoliang B6 were evaluated using tea residue as a medium. P. pulmonarius and L. sajor-caju (Fr.) exhibited the fastest growth rates, with colony radii of 33.1 and 28.5 mm, respectively. M. kaoliang B6 demonstrated substantial degradation abilities for cellulose, hemicellulose, and lignin, with decolorization radii of 12.2, 0.9, and 8.5 mm, respectively. After a 9-days liquid fermentation, M. kaoliang B6 achieved the highest conversion efficiency at 27.8%, attributed to its high cellulase (191 U∙mL −1 ) and lignin peroxidase (36.9 U∙L −1 ) activities. P. pulmonarius and L. sajor-caju (Fr.) showed lower conversion rates of 8.6% and 3.8%, despite having high hemicellulase activities (67.1 and 70.9 U∙mL −1 ). Fermentation by M. kaoliang B6 resulted in a reduction of protein and total sugar content in the tea residue by 174 and 192 mg g −1 , by which the mycelium's protein and total sugar content increased by 73 and 188 mg g −1 . Co-fermentation of these three strains had little effect on the improvement of conversion efficiency, which might owe to the antagonistic interactions among the strains. Generally, utilizing tea residue for edible fungi fermentation is a sustainable process for bio-waste treatment, enabling efficient nutrient conversion under mild conditions without adding chemicals.
研究领域:
微生物学
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生物技术
·
废物管理
·
真菌生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）