MECHANISMS OF AGEING AND DEVELOPMENT
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2024
·
IF 5.3
An-Guo Wu,Yuan-Yuan Yong,Chang-Long He,Ya-Ping Li,Xing-Yue Zhou,Lu Yu,Qi Chen,Cai Lan,Jian Liu,Chong-Lin Yu,Da-Lian Qin,Jian-Ming Wu,Xiao-Gang Zhou
Pharmacological strategies to delay aging and combat age-related diseases are increasingly promising. This study explores the anti-aging and therapeutic effects of two novel 18-norspirostane steroidal saponins from Trillium tschonoskii Maxim, namely deoxytrillenoside CA (DTCA) and epitrillenoside CA (ETCA), using Caenorhabditis elegans ( C. elegans ). Both DTCA and ETCA significantly extended the lifespan of wild-type N2 worms and improved various age-related phenotypes, including muscle health, motility, pumping rate, and lipofuscin accumulation. Furthermore, these compounds exhibited notable alleviation of pathology associated with Parkinson's disease (PD) and Huntington's disease (HD), such as the reduction of α-synuclein and poly40 aggregates, improvement in motor deficits, and mitigation of neuronal damage. Meanwhile, DTCA and ETCA improved the lifespan and healthspan of PD- and HD-like C. elegans models. Additionally, DTCA and ETCA enhanced the resilience of C. elegans against heat and oxidative stress challenges. Mechanistic studies elucidated that DTCA and ETCA induced mitophagy and promoted mitochondrial biogenesis in C. elegans , while genetic mutations or RNAi knockdown affecting mitophagy and mitochondrial biogenesis effectively eliminated their capacity to extend lifespan and reduce pathological protein aggregates. Together, these compelling findings highlight the potential of DTCA and ETCA as promising therapeutic interventions for delaying aging and preventing age-related diseases.
研究领域:
衰老
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神经退行性疾病
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药理学
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分子生物学
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遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Wang Liu,Ya-Min Qin,Jin-Yang Shi,De-Ling Wu,Chun-Yang Liu,Juan Liang,Song-Zi Xie
This study aimed to evaluate the influence of ultrasonic degradation on the physicochemical and biological characteristics of Polygonatum cyrtonema polysaccharide (PCP, 8.59 kDa). PCP was subjected to ultrasonic treatment for 8, 16, and 24 h and yielded the degraded fractions PCP-8, PCP-16, and PCP-24 (5.06, 4.13, and 3.69 kDa), respectively. Compared with the intact PCP, PCP-8, PCP-16 and PCP-24 had a reduced particle size (decrements of 28.03 %, 46.15 % and 62.54 %, respectively). Although ultrasonic degradation did not alter the primary structure of PCP, its triple helical and superficial structures were disrupted, with degraded fractions demonstrating reduced thermal stability and apparent viscosities compared with those of the intact PCP. Furthermore, the functional properties of the degraded fractions were different. PCP-16 most favourably affected GLP-1 secretion, while PCP-8 and PCP-24 exhibited the strongest antioxidant and enzyme inhibitory activities, respectively. Hence, controlled ultrasound irradiation is an appealing approach for partially degrading PCP and enhancing its bioactivity as a functional agent.
研究领域:
生物化学
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药理学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
STARCH-STARKE
·
2024
·
IF 2.6
Yanhua Tian,Aiguo Luo,Zhaoyan Yang,Ling Zhang,Xuanping Zhang
Ultrasound assisted aqueous two-phase extraction (UATPE) is used to extract polysaccharides from Ophiopogon japonicus (OJPs). Experimental factors in UATPE process are optimized by response surface methodology. The optimal extraction conditions are obtained as follows: the ethanol concentration of 34%, ammonium sulfate concentration of 23%, solid-to-liquid ratio of 1:17 g/mL, and ultrasound power of 480 W, and the yield of OJPs is 9.41% ± 0.12%, and then the crude OJPs are further purified by different column chromatography to obtain a homogenous fraction (OJPs-2-SG), and identified its structure via different methods. OJPs-2-SG with a molecular weight of 1.25 × 105 Da is comprised of mannose (Man), galactose (Gal), glucose (Glc), and rhamnose (Rha) with a molar ratio of 5.15: 26.39: 68.72:8.41. Moreover, OJPs-2-SG contained α and β glycosidic bonds, and didn't showed a triple helix structure. Furthermore, OJPs-2-SG described irregular structures, and cylindrical and conical block like features. The IC50 values of scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH), and superoxide (O2-) radicals for OJPs-2-SG achieved 3.72 mg/mL, 3.89 mg/mL, and 8.71 mg/mL, respectively. These results provide important references for the extraction and application of polysaccharides.
研究领域:
植物化学
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食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Weiguang Luo,Xiuwei Li,Chenxi Zhang,Kai Shen,Mengxue Li,Yan Zhuang,Jinhai Huo,Weiming Wang
Polygonatum cyrtonema (PC) is a traditional Chinese herb used in both food and medicine with considerable bioactivity. The nine-steam processed pieces of PC are the main form of clinical application, while research has focused on the polysaccharide of their crude form. This study evaluated the physicochemical characteristics and protective effects of polysaccharides from both crude PC (PCP) and nine-steamed PC (NPCP) against cyclophosphamide (CTX)-induced immunosuppression. The findings indicate significant differences in the physicochemical characteristics of PCP and NPCP. The nine steaming treatments reduced the polysaccharide content by 8.15 %, and significantly altered monosaccharide proportion. Both polysaccharides accelerated the recovery of spleen and thymus indexes and restored the levels of TNF-α, IL-1β, IL-6, IgA, IgM, and IgG in the serum of CTX-treated mice in a dose-dependent manner as well as improved the morphology of spleen, thymus, and colon tissues. Furthermore, PCP and NPCP promoted the production of SCFAs and the recovery of intestinal flora in CTX-induced immunosuppressed mice. NPCP exhibited a better protective effect against CTX-induced immunosuppression. The results of this study confirm the importance of the nine-steaming method for PC and provide a basis for the further development of NPCP as a functional food to alleviate CTX-induced immunosuppression.
研究领域:
药理学
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免疫学
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中医药学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
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2021
·Vol. 11
·期 60
·37952-37965
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IF 3.361
Jin-Bo Bai,Ji-Chun Ge,Wang-Juan Zhang,Wang Liu,Jian-Ping Luo,Feng-Qing Xu,De-Ling Wu,Song-Zi Xie
Polygonatum species, including P. cyrtonema, P. kingianum, and P. sibiricum, are edible plants with medicinal purposes, which have long been consumed as food due to their high nutritional value. In this study, polysaccharides from P. cyrtonema (PCP), P. kingianum (PKP) and P. sibiricum (PSP) were obtained, and their physicochemical properties and in vitro biological activities were investigated. Our results demonstrated that PCP, PKP, and PSP consist of major fructose and minor glucose, galacturonic acid, and galactose in different molar ratios with the molecular weights of 8.5 × 103 Da, 8.7 × 103 Da, and 1.0 × 104 Da, respectively. The three polysaccharides had triple-helical structures with β-D-Fruf, α-D-Glcp, α-D-Galp sugar residues, and an O-acetyl group, and displayed peak-shaped structures in different sizes. They also exhibited thermal, shear-thinning behavior and viscoelastic properties, and PCP presented the highest viscoelasticity. Moreover, they exerted strong free radical-scavenging abilities, and significant reducing capacity. PCP was the strongest, followed by PSP and then PKP. They significantly promoted the polarization of the M1 macrophage, with the effect of PCP ranking first. All three had similar effects on GLP-1 secretion. It is, therefore, necessary to identify the various roles of these three Polygonatum polysaccharides as functional agents based on their bioactivities and physicochemical properties.
研究领域:
药理学
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生物化学
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食品科学
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植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Jia-Rui Liu,Bo-Xue Chen,Jia-Qi Huang,Xue Li,Tian-Yi Cui,Bin Lv,Zhi-Fei Fu,Xin Zhao,Wen-Zhi Yang,Xiu-Mei Gao
Polygonatum odoratum (Yu-Zhu) can be utilized to treat the digestive and respiratory illness . Previous studies have revealed that the underlying therapeutic mechanism of P. odoratum polysaccharides (POPs) is associated with remodeling the gut microbiota . However, POPs in terms of the chemical composition and fermentation activities have been understudied. Here we developed the three-level fingerprinting approaches to characterize the structures of POPs and probed into the beneficial effects on promoting the growth and fermentation of Lactobacillus johnsonii . POPs were prepared by water decoction followed by alcohol sedimentation, while trifluoroacetic acid under different conditions to prepare the hydrolyzed oligosaccharides and monosaccharides . POPs exhibited three main molecular distribution of 601–620 kDa, 4.12–6.09 kDa, and 3.57–6.02 kDa. Hydrolyzed oligosaccharides with degree of polymerization (DP) 2–13 got primarily characterized by analyzing the rich fragmentation information obtained by hydrophilic interaction chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (HILIC/IM-QTOF-MS). Amongst them, the DP5 oligosaccharide was characterized as 1,6,6-kestopentaose. The molecular ratio of Fru: Ara : Glc: Gal : Xyl was 87.72: 0.30: 11.56: 0.19: 0.23. In vitro fermentation demonstrated that 4.5 mg/mL of POPs could significantly promote the growth of L. johnsonii . Co-cultivated with 4.5 mg/mL of POPs, L. johnsonii exhibited stronger antimicrobial activity against Klebsiella pneumoniae . The concentrations of short-chain fatty acids in the POPs-lactobacilli fermented products, including acetic acid, isobutyric acid , and isovaleric acid , were increased. Conclusively, POPs represent the promising prebiotic candidate to facilitate lactobacilli , which is associated with exerting the health benefits.
研究领域:
药理学
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微生物学
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分析化学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Meixia Zheng,Hailan Su,Rongfeng Xiao,Jianfu Chen,Hong Chen,Kok Bing Tan,Yujing Zhu
A novel film composed of Polygonatum cyrtonema extracts (PCE), xanthan gum (XG), flaxseed gum (FG) and carboxymethyl cellulose (CMC) was prepared (XFCP). Addition of PCE has decreased the light transmittance, while enhanced the UV blocking performance, antioxidant activity, tensile strength and elongation at break of XFCP due to polysaccharides, polyphenols, and flavonoid in PCE. Structural analyses by FTIR and XRD indicated the hydrogen-bonding interaction between PCE, XG , FG and CMC . It was found that compared with the control sample, XFCP2.5% with the lowest WVTR was able to prolong the shelf life of mango. The overall quality of mango was also improved in terms of lower decay rate, weight loss rate, total soluble solid, and polyphenol oxidase, higher titratable acidity, V c , and superoxide dismutase than control mango upon 8 days of storage. This effectively expanded the application of PCE into food packaging in addition to merely as Chinese traditional medicine herbs.
研究领域:
食品科学
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）