FOOD SCIENCE AND BIOTECHNOLOGY
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2025
·
IF 3.1
Nho Soo Bin,Do Sang-Hun,Oh Seongjin,Park Yong-Cheol,Kim Sun-Ki
This study aimed to develop a non-genetically modified organism (non-GMO) Saccharomyces cerevisiae strain with enhanced glutathione production using UV-induced random mutagenesis. Among 30 wild-type strains, S. cerevisiae KACC 48331 was selected for its high glutathione production. A #14 mutant with significantly higher glutathione content was isolated via acrolein resistance screening. Optimal batch fermentation was established at 20 °C and pH 4.5 using a cost-effective medium of molasses and corn steep liquor (CSL). In fed-batch fermentation, the #14 mutant achieved 1.98 g/L of glutathione using molasses and CSL as carbon and nitrogen sources, comparable to yields from expensive complex media containing peptone and yeast extract. Notably, over 99% of the glutathione produced was in the reduced, biologically active form. These results demonstrate the feasibility of using non-GMO yeast and industrial by-products for sustainable and economical glutathione production.
研究领域:
生物技术
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微生物发酵
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工业微生物学
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代谢工程
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应用微生物学
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DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2025
·
IF 4
Yilian Li,Jing Wu,Pengfei Wu,Chen Wang,Ruichao Huang,Jianan Zhang
The livestock industry has undergone rapid expansion in recent years, resulting in an increased demand for feed protein. The production of plant and animal proteins cannot sustainably meet the expanding demand for protein, and the development of single cell protein (SCP) as a feed protein is a promising avenue. Molasses is a cheap and nutritious by-product of sugar production from sugarbeet and sugarcane, and is one of the common substrates available to microorganisms. In this study, R. glutinis As 2.703 was used to produce SCP by fermentation with inexpensive carbon source molasses and nitrogen source corn steep liquor instead of glucose and yeast extract, respectively. The results revealed that when the concentration of molasses in the substrate was 61 g/L, the biomass concentration reached 21.0 g/L, and the protein, lipid, carotenoid contents in dry cell were 30.4 %, 7.8 %, and 31.7 μg/g, respectively. SuperPro Designer was used to simulate the process and carry out the economic analysis for producing SCP from 100,000 tons of molasses annually. A plant of this size could produce 32,908 tons/year of SCP at cost of 988.2 USD/ton with payback period of 6.3 years. Sensitivity analysis showed that the selling price of SCP has the greatest impact on the economics of industrial production of SCP, and that by increasing its protein and carotenoid content, its value can be increased, and further increasing its selling price is the key to achieving industrialisation. This study provides new ideas to cope with the future protein feed crisis.
研究领域:
生物技术
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微生物学
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农业工程
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食品科学
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经济分析
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产品关联
DOI
高影响因子
Fermentation-Basel
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2025
·Vol. 11
·期 3
·119
·
IF 3.3
Liang Wu,Yong Xu
Bacillus subtilis(B. subtilis) has booming application prospects in the feed industry as a value-added probiotic. Considering the intermediate and end saccharides of glucan and xylan digestion during lignocellulose biorefinery processing, the various digested saccharides were detected to disclose the bio-uptake and fermenting characteristics by solo or co-fermentation, especially with respect to five monosaccharides, cellobiose, and functional food additive—xylo-oligosaccharides (XOS).B. subtiliscan directly ferment XOS without xylose, achieving a cell yield of 0.71 g/g, while it hardly utilizes cellobiose except glucose co-fermentation. With a 5 g/L glucose addition and co-fermentation, bacterial cell yield was improved to 0.70 g/g. In total,B. subtilishad a 0.56–0.90 g/g cell yield from corncob-digested saccharides and the highest saccharide-uptaking rate of 98%. This study provides critical insights and experimental data to advance lignocellulosic biorefinery technologies, particularly for probiotics production and simplification of enzymatic hydrolysis, as well as the potential application in high value-added XOS biopurification.
研究领域:
生物工程
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微生物学
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产品关联
DOI
高影响因子
Biotechnology for Biofuels and Bioproducts
·
2024
·Vol. 17
·期 1
·1-12
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IF 3.3
Zhang Bin,Gou Kexin,Xu Kexin,Li Zhimin,Guo Xiaoyan,Wu Xiaoyu
Background β-Arbutin, a hydroquinone glucoside found in pears, bearberry leaves, and various plants, exhibits antioxidant, anti-inflammatory, antimicrobial, and anticancer effects. β-Arbutin has wide applications in the pharmaceutical and cosmetic industries. However, the limited availability of high-performance strains limits the biobased production of β-arbutin. Results This study established the β-arbutin biosynthetic pathway in C. glutamicum ATCC13032 by introducing codon-optimized ubiC , MNX1 , and AS . Additionally, the production titer of β-arbutin was increased by further inactivation of csm and trpE to impede the competitive metabolic pathway. Further modification of the upstream metabolic pathway and supplementation of UDP-glucose resulted in the final engineered strain, C. glutamicum AR11, which achieved a β-arbutin production titer of 7.94 g/L in the optimized fermentation medium. Conclusions This study represents the first successful instance of de novo β-arbutin production in C. glutamicum , offering a chassis cell for β-arbutin biosynthesis.
研究领域:
生物技术
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代谢工程
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药学
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化妆品科学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Zhenqiang Zhao,Jiajia You,Xuanping Shi,Mengmeng Cai,Rongshuai Zhu,Fengyu Yang,Meijuan Xu,Minglong Shao,Rongzhen Zhang,Youxi Zhao,Zhiming Rao
The rapid development of high-productivity strains is fundamental for bio-manufacture in industry. Here, Multi-module metabolic engineering was implemented to reprogram Escherichia coli , enabling it to rapidly transitioning from zero-producer to hyperproducer of L-threonine. Firstly, the synthesis pathway of L-threonine was rationally divided into five modules, and the rapid production of L-threonine was achieved by optimizing the expression of genes in each module. Subsequently, the capture and fixation of CO 2 was enhanced to improve L-threonine yield. Dynamically balancing cell growth and yield by quorum-sensing system resulted in the accumulation of L-threonine up to 34.24 g/L. Ultimately, the THR36-L19 strain accumulated 120.1 g/L L-threonine with 0.425 g/g glucose in a 5 L bioreactor. This is the highest yield for de novo producing L-threonine reported to date and without the use of exogenous inducers and antibiotics in the fermentation process. It also provided an effective technological guidence for the zero-to-overproduction of other chemicals.
研究领域:
代谢工程
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生物技术
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微生物学
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生化工程
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2015
·Vol. 177
·期 6
·1229-1240
·
IF 1.735
Yu Xin-Jun,Yu Zhi-Qiang,Liu Ying-Liang,Sun Jie,Zheng Jian-Yong,Wang Zhao
High-fructose corn syrup (HFCS) is an agro-source product and has been the most commonly used substitute for sugar as sweetener in food industry due to its low price and high solution property. In this study, the F55 HFCS, rich in fructose and glucose, was first tested for biomass and docosahexaenoic acid productions as a mixed carbon source by a newly isolated Aurantiochytrium sp.YLH70. After the compositions of the HFCS media were optimized, the results showed that the HFCS with additions of metal ion and vitamin at low concentrations was suitable for biomass and docosahexaenoic acid productions and the metal ion and sea salt had the most significant effects on biomass production. During the 5-l fed-batch fermentation, total HFCS containing 180 g l −1 reducing sugar was consumed and yields of biomass, lipid, and DHA could reach 78.5, 51, and 20.1 g l −1 , respectively, at 114 h. Meanwhile, the daily productivity and the reducing sugar conversion yield for docosahexaenoic acid were up to 4.23 g l −1 day −1 and 0.11 g g −1 . The fatty acid profile of Aurantiochytrium sp.YLH70 showed that 46.4 % of total fatty acid was docosahexaenoic acid, suggesting that Aurantiochytrium sp.YLH70 was a promising DHA producer.
研究领域:
微生物学
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生物技术
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海洋生物技术
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DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2019
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IF 4.259
Kang Yajing,Wang Yingying,Hou Bingbing,Wang Ruida,Ye Jiang,Zhu Xiaoyu,Wu Haizhen,Zhang Huizhan
Lincomycin is one of the most important antibiotics in clinical practice. To further understand the regulatory mechanism on lincomycin biosynthesis, we investigated a pleiotropic transcriptional regulator AdpAlin in the lincomycin producer Streptomyces lincolnensis NRRL 2936. Deletion of adpAlin (which generated ΔadpAlin) interrupted lincomycin biosynthesis and impaired the morphological differentiation. We also found that putative AdpA binding sites were unusually scattered in the promoters of all the 8 putative operons in the lincomycin biosynthetic gene cluster (BGC). In ΔadpAlin, transcript levels of structural genes in 8 putative operons were decreased with varying degrees, and electrophoretic mobility shift assays (EMSAs) confirmed that AdpAlin activated the overall putative operons via directly binding to their promoter regions. Thus, we speculated that the entire lincomycin biosynthesis is under the control of AdpAlin. Besides, AdpAlin participated in lincomycin biosynthesis by binding to the promoter of lmbU which encoded a cluster sited regulator (CSR) LmbU of lincomycin biosynthesis. Results of qRT-PCR and catechol dioxygenase activity assay showed that AdpAlin activated the transcription of lmbU. In addition, AdpAlin activated the transcription of the bldA by binding to its promoter, suggesting that AdpAlin indirectly participated in lincomycin biosynthesis and morphological differentiation. Uncommon but understandable, AdpAlin auto-activated its own transcription via binding to its own promoter region. In conclusion, we provided a molecular mechanism around the effect of AdpAlin on lincomycin biosynthesis in S. lincolnensis, and revealed a cascade regulation of lincomycin biosynthesis by AdpAlin, LmbU, and BldA.
研究领域:
微生物学
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分子生物学
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抗生素研究
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产品关联
DOI
PubMed
高影响因子
Synthetic and Systems Biotechnology
·
2020
·
IF 0
Ruida Wang,Fanjing Kong,Haizhen Wu,Bingbing Hou,Yajing Kang,Yuan Cao,Shiwei Duan,Jiang Ye,Huizhan Zhang
The lincosamide family antibiotic lincomycin is a widely used antibacterial pharmaceutical generated by Streptomyces lincolnensis , and the high-yield strain B48 produces 2.5 g/L lincomycin, approximately 30-fold as the wild-type strain NRRL 2936. Here, the genome of S. lincolnensis B48 was completely sequenced, revealing a ~10.0 Mb single chromosome with 71.03% G + C content. Based on the genomic information, lincomycin-related primary metabolism network was constructed and the secondary metabolic potential was analyzed. In order to dissect the overproduction mechanism, a comparative genomic analysis with NRRL 2936 was performed. Three large deletions (LDI-III), one large inverted duplication (LID), one long inversion and 80 small variations (including 50 single nucleotide variations, 13 insertions and 17 deletions) were found in B48 genome. Then several crucial mutants contributing to higher production phenotype were validated. Deleting of a MarR-type regulator-encoding gene slinc377 from LDI, and the whole 24.7 kb LDII in NRRL 2936 enhanced lincomycin titer by 244% and 284%, respectively. Besides, lincomycin production of NRRL 2936 was increased to 7.7-fold when a 71 kb supercluster BGC33 from LDIII was eliminated. As for the duplication region, overexpression of the cluster situated genes lmbB2 and lmbU , as well as two novel transcriptional regulator-encoding genes ( slinc191 and slinc348 ) elevated lincomycin titer by 77%, 75%, 114% and 702%, respectively. Furthermore, three negative correlation genes ( slinc6156 , slinc4481 and slinc6011 ) on lincomycin biosynthesis, participating in regulation were found out. And surprisingly, inactivation of RNase J-encoding gene slinc6156 and TPR (tetratricopeptide repeat) domain-containing protein-encoding gene slinc4481 achieved lincomycin titer equivalent to 83% and 68% of B48, respectively, to 22.4 and 18.4-fold compared to NRRL 2936. Therefore, the comparative genomics approach combined with confirmatory experiments identified that large fragment deletion, long sequence duplication, along with several mutations of genes, especially regulator genes, are crucial for lincomycin overproduction.
研究领域:
微生物学
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基因组学
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药学
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分子生物学
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产品关联
DOI
PubMed
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Xutong Ma,Xinlu Liu,Yong Xu
The probiotic of Bacillus subtilis ( B.subtilis ) is widely used in animal husbandry as a promising alternative to antibiotics, and a high value-added bioproduct from abundant and low-cost agricultural straws. For efficient fermentation of xylose-rich corncob slurry to B. subtilis cells, the step-by-step fermentation process (SSFP) and one-step fermentation process (OSFP) were proposed and compared by the combined technology of sulfuric acid pretreatment and enzymatic hydrolysis with cellulase . Xylose and glucose were fermented separately and sequentially in the SSFP route, while they were co-fermented in OSFP route. Here, B. subtilis performed an efficient bioconversion and almost fermented simultaneously glucose, xylose and arabinose in corncob slurry. We could obtain 326.8 g of B. subtilis in SSFP and 323.7 g probiotic cells in OSFP from 1000 g of corncob, respectively. In general, the OSFP route was preferable by virtue of a straightforward process and simple operation.
研究领域:
微生物学
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生物工程
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产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
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2022
·
IF 9.224
Ze-Wang Guo,Zi-Fu Ni,Min-Hua Zong,Wen-Yong Lou
Consolidated bioprocessing (CBP) has emerged as a promising strategy for green synthesis of 2,3-butanediol (BDO) from Jerusalem artichoke tubers (JAT). Here, modular metabolic engineering of Bacillus licheniformis MW3 was implemented, in which the overall BDO synthesis process was divided into four modules (inulin hydrolysis, sporulation elimination, carbon flux redistribution, and enantiopure meso-BDO synthesis). First, endo-inulinase was introduced to boost inulin utilization. Second, sporulation was blocked by spoIIE knockout, thus reducing the leakage risk of the genetically engineered strain from the fermentation plant. Third, more carbon flux was shunted into BDO production via exchanging the alsS start codon, blocking acetoin catabolism, and competing pathways. Finally, enantiopure meso-BDO was synthesized by gdh knockout. Encouragingly, the final engineered strain, MWO-8, produced meso-BDO with 99.3% purity at a titer, productivity, and yield of 82.51 g/L, 1.38 g/L/h, and 0.439 g/g, respectively. To our knowledge, this is the highest titer of BDO from JAT by CBP so far. Collectively, this work provides a highly promising alternative approach for sustainable and cost-effective production of BDO.
研究领域:
生物技术
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代谢工程
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生化工程
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微生物学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Jian Han,Feng Han,Jiaqing Wang,Yang Wei,Boris N. Kuznetsov,Yong Xu
Oxygen supply is one of the decisive factors of obligate aerobic microbial fermentation. Gluconobacter oxydans as a typical aerobic bacterium has excellent whole-cell catalysis and bio-oxidation ability to sugars and alcohols. By the dual function of oxygenation and alkali production of sodium percarbonate (SPC), the dissolved oxygen supply and pH stabilization was experimentally investigated and applied for the first time in G. oxydans fermentation. SPC performed a significant assisting effort to bio-oxidation performance by the in-situ oxygen supply and neutralization for whole-cell catalysis and bio-oxidation of lignocellulosic xylose , glucose, and glycerol. Compared with common aeration fermentation (and even the sealed pure-oxygen supplied mode), the bio-production of the three platform compounds xylonic acid, gluconic acid , and 1, 3-dihydroxyacetone was improved significantly by 45.7%, 28.4%, and 39.1%, respectively. In addition, the direct bioproduction of xylonic acid from the crude corncob sulfate hydrolysate was also improved by 17.3%. Within 4 h, 37.7 g/L of xylonic acid was produced in the fermentation tank, with a productivity of 9.4 g/L/h. The in-situ SPC assistance provides a simple and practical approach to intensify the bio-oxidation performance of G. oxydans for the whole-cell catalysis of high-value-added platform compounds from lignocellulosic feedstocks .
研究领域:
微生物学
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生物工程
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发酵工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）