SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Yue Wang,Yunlei Zhou,Lv Xu,Zhenwei Han,Huanshun Yin,Shiyun Ai
Zeatin is a kind of typical cytokinin, which plays important roles in regulating the proliferation and differentiation of plant cells, and widely distributed in various plant tissues. Herein, a simple and sensitive photoelectrochemical method was developed for zeatin detection using graphite-like carbon nitride (g-C 3 N 4 ) as photoactive material, graphene quantum dots as photoactivity improvement reagent, gold nanoparticles as immobilization substrate for DNA probe, DNA biotin labeled aptamer as zeatin recognition and streptavidin capture element, and streptavidin as PEC response inhibitor factor. In the presence of zeatin, DNA aptamer can bind with zeatin to form conjugate, which make aptamer releasing from electrode surface and decrease the capture amount of streptavidin, causing the increase of the PEC response. Under optimal conditions, the biosensor showed wide linear range from 0.1 to 100 nM and low detection limit of 0.031 nM (3σ). The developed method also presented good selectivity, even discriminating zeatin analogues. In addition, the proposed detection strategy can be further applied to detect zeatin in complex biological matrix, revealing great potential in practical applicability. This work might provide an excellent platform for zeatin or other phytohormone detection using aptamer as recognition reagent.
研究领域:
植物科学
·
分析化学
·
生物化学
·
纳米技术
✓ 已验证
DOI
高影响因子
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
·
2018
·Vol. 65
·期 3
·372-383
·
IF 0.816
Zhang Y.,Yong Y. B.,Wang Q.,Lu Y. M.
Lily is regarded as one kind of the most economically important cut flowers in China. In this paper, lily cultivar ‘Aladdin’ ( Lilium longiflorum × Lilium asiatic ) was chosen as experimental material for its distinct high propagation coefficient in nature and three main conclusions were achieved. Firstly, our observation results revealed that the noticeable propagation coefficient of ‘Aladdin’ depended on strong capacity to form many underground stem axillary bulbils positioned on underground node of plant after 70 days of cultivation and having 1 cm flower buds. Secondly, in terms of physiological changes, zeatin riboside (ZR) relative concentration, soluble sugar and starch content peak when and where such bulbils are forming. Finally, two KNOX family Lilium genes were sequenced completely and one BELL family gene was sequenced partly; besides, there might be an interaction between KNOX-2 and BELL protein to accomplish their functions in bulbils organogenesis according to qRT-PCR results. As far as we know, it is the first time to study the formation mechanism of lily underground stem axillary bulbils. We illustrated physiological and molecular profiles, as well as offered a new prospect to lily molecular breeding in the future.
研究领域:
植物学
·
分子生物学
·
植物生理学
·
遗传学
✓ 已验证
DOI
高影响因子
Agronomy-Basel
·
2024
·Vol. 14
·期 10
·2450
·
IF 3.3
Ke Li,Yunji Xu,Dalu Gu,Xiaodong Yin,Yanyan Jia,Tinggang Wen,Weiqing Jiang,Yang Che,Qisheng Li,Zhangrong Wen,Xiaofeng Du,Wenfei Yang
This paper aims to clarify the effects of different exogenous phytohormones on the physiological traits of rice (Oryza sativaL.) at the early stage of irrigation under low-temperature stress. In this study, two types of rice varieties with different temperature sensitivities screened out previously, namely, a cold-tolerant variety (Nan Jing 9108) and a low-temperature-sensitive variety (Hui Liang You 898), were used in pots to simulate the process of low-temperature stress in rice at the early stage of grouting (6–9 days after anthesis) with artificial low-temperature treatments. The experimental treatments were 450 mg L−1Methyl jasmine acid (MJ), 46 mg L−1Melatonin (MT), 69 mg L−1Salicylate (SA), 40 mg L−1Erythromycin (GA3), 25 mg L−1Zeatin (Z), 145 mg L−1Spermidine (SPD), and 5 mg L−1Abscisic acid (ABA) sprayed on rice before low-temperature stress, while low-temperature treatment without spraying (DK) and conventional planting without spraying (CK) were added as the control. The results showed that compared with the room temperature control (CK, sprayed with deionized water), the low-temperature control (DK, low-temperature treatment, and sprayed with deionized water) all significantly reduced the rice grain yield. Different exogenous hormones sprayed before low-temperature stress could increase rice yield, among which, Z and SPD spraying treatments had a better effect on the yield of Hui Liang You 898, while different exogenous hormone treatments increased the yield of Nan Jing 9108 in an average manner. The Z and SPD treatments increased the yield of Hui Liang You 898 by 24.87% and 26.16% and that of Nan Jing 9108 by 15.87% and 17.80%, respectively. This was mainly attributed to the significant increase in thousand-grain weight and fruiting rate, while there was no significant difference in the number of spikes and number of grains. The different exogenous hormone treatments were able to delay leaf senescence, enhance the photosynthetic production capacity of plants by increasing leaf chlorophyll content, and thus increase the accumulation of photosynthetic assimilation products and population growth rate after flowering. Among them, both Z and SPD treatments resulted in a population growth rate of more than 30% from spike flushing to maturity, which led to a higher dry matter accumulation of the plant at maturity. In addition, in the dry matter distribution of the plant at maturity, the seeds occupied a higher accumulation amount and proportion compared with the respective DK; the SPD treatment resulted in the maximum distribution rate of seeds at maturity of Hui Liang You 898, with an increase of 8.27%, and the Z treatment resulted in the maximum distribution rate of seeds at maturity of Nan Jing 9108, with an increase of 7.34%. At the same time, the Z treatment significantly increased the activities of adenosine diphosphate glucose phosphorylated enzyme (AGP) and starch branching enzyme (SBE) in the grains of both varieties, which resulted in the accumulation of more starch and ultimately increased the rice grain yield. The results verified that different exogenous phytohormones could be used to regulate the insufficiency of grouting caused by low-temperature stress during the grouting and fruiting stages of rice and enriched their agronomic and physiological traits in response at the same time.
研究领域:
农学
·
植物生理学
·
逆境生物学
✓ 已验证
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Xinyi Zhang,Jiakun Zhou,Zemin Li,Yimin Qin,Ruitao Yu,Huaiwei Zhang,Yuhong Zheng,Jiangwei Zhu,Demeng Zhang,Li Fu
Seaweed fertilizer refers to fertilizer created by extracting the active ingredients from raw seaweed that is then applied to plants as a nutrient source to promote growth and increase yield. A quick method of analysing seaweed fertilizer quality has practical value. In this work, we established a simple electrochemical method for analysing commercial seaweed fertilizer by comparing the contributions of each electro-active ingredient. The electrochemical profiles of 3-hydroxyphenylacetic acid, gibberellin, lycine, abscisic acid, indole-3-acetic acid and zeatin were first recorded in a database. Then, the electrochemical profiles of four commercial seaweed fertilizers were recorded and examined to analyse the contribution of each ingredient.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
·
2023
·Vol. 148
·期 6
·276-282
·
IF 1.9
Ziying Jiang,Tongrui Liu,Qi Zhang,Lili Dong,Xinyi Deng
The branch number of plants is an important agronomic trait that directly influences the ornamental characters and production costs of ornamental plants. Shoot branching has always been a hot topic for Petunia hybrida. During our research, we isolated the homologous gene of narrow-leaf 1 (NAL1), denoted as PhNAL1. The expression level of PhNAL1 was higher in leaves and axils than in roots, stems, and flowers. Pertinent to shoot apex removal and 6-benzyladenine treatments, both interventions demonstrated a suppressive effect on the expression of PhNAL1. Through subcellular localization analysis, we found that PhNAL1 predominantly localized in the nucleus. By using RNA interference targeting PhNAL1, we induced a noticeable increase in branch number while concurrently reducing plant height of petunia. These findings demonstrate that PhNAL1 is involved in regulating branch development within petunia. This study provides genetic resources for the subsequent cultivation of new cultivars of petunia endowed with distinct branching characteristics.
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2018
·Vol. 143
·期 21
·5185-5190
·
IF 3.864
Yunlei Zhou,Huanshun Yin,Yue Wang,Chengji Sui,Minghui Wang,Shiyun Ai
A simple and sensitive electrochemical aptasensor was constructed for zeatin detection, where MoS2 nanosheets were used as the immobilization matrix for gold nanoparticles (AuNPs), and AuNPs were employed as the immobilization matrix to probe DNA. After the aptamer DNA and assist DNA hybridized with probe DNA, Y-type DNA can be formed with two biotins at the terminals of aptamer DNA. Then, avidin modified alkaline phosphatase (Avidin-ALP) can be further modified on the electrode surface through the biotin and avidin interaction. Under the catalytic effect of ALP, p-nitrophenylphosphate disodium (PNPP) can be hydrolyzed to produce p-nitrophenol (PNP). However, in the presence of zeatin, the formed Y-type DNA can be destroyed due to the formation of the zeatin–aptamer conjugate, which further reduces the amount of PNP and leads to the decrease of the oxidation signal of PNP. Under the optimum conditions, the change of the oxidation peak current of PNP was inversely proportional to the logarithm value of zeatin concentration in the range of 50 pM–50 nM. The detection limit was calculated to be 16.6 pM. This electrochemical method also showed good detection selectivity and stability. The potential applicability of this method was proved by detecting zeatin in real samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）