Foods
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2022
·Vol. 11
·期 22
·3695
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IF 5.561
Jiamin Dang,Shuangkui Du,Liying Wang
The objective of this study was to investigate the soluble epoxide hydrolase (sEH) inhibitory properties of corn gluten peptides. In total, 400 dipeptides and 8000 tripeptides were first virtually screened by molecular docking and 30 potential sEH inhibitory peptides were selected. Among them, WEY, WWY, WYW, YFW, and YFY showed the highest sEH inhibitory activities with IC50values of 55.41 ± 1.55, 68.80 ± 7.72, 70.66 ± 9.90, 96.00 ± 7.5, and 94.06 ± 12.86 μM, respectively. These five peptides all behaved as mixed-type inhibitors and were predicted to form hydrogen bond interactions mainly with Asp333, a key residue located in the catalytic active site of sEH. Moreover, it was found that the corn gluten hydrolysates of Alcalase, Flavourzyme, pepsin and pancreatin all exhibited high sEH inhibitory activities, with IC50values of 1.07 ± 0.08, 1.19 ± 0.24, and 1.46 ± 0.31 mg/mL, respectively. In addition, the sEH inhibitory peptides WYW, YFW, and YFY were successfully identified from the corn gluten hydrolysates by Alcalase using nano-LC-MS/MS. This study demonstrated the sEH inhibitory capacity of peptides for the first time and corn gluten might be a promising food protein source for discovering novel natural sEH inhibitory peptides.Keywords:corn gluten;peptide;soluble epoxide hydrolase;inhibitor;molecular docking
研究领域:
生物化学
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食品科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Cuiling Shang,Yansong Li,Qi Zhang,Song Tang,Xiaoqian Tang,Honglin Ren,Pan Hu,Shiying Lu,Peiwu Li,Yu Zhou
Zearalenone (ZEN), as one of the most important foods and feeds pollutant mycotoxins , is threatening human and animal health. Here, we have developed a dual-signal immunoassay triggered by alkaline phosphatase (ALP) for detecting ZEN in cornmeal. The immobilized ZEN-bovine serum albumin and free ZEN in the sample were competitively bound to an anti-ZEN monoclonal antibody (McAb). Then, the ALP labeled goat anti-mouse IgG (ALP-IgG) combined with the McAb. ALP catalyzed ascorbic acid 2-phosphate to produce L -ascorbic acid (AA). AA converted potassium ferricyanide (K 3 [Fe(CN) 6 ]) to potassium ferrocyanide (K 4 [Fe(CN) 6 ]), which reacted with ferric ion (III) to promote the formation of Prussian blue nanoparticles (PB NPs). Consequently, the solution appeared multicolor changes. Meanwhile, PB NPs had a maximum absorption peak at 700 nm. It could be monitored by an UV–vis spectrometer. As an electron transfer medium, K 3 [Fe(CN) 6 ] was consumed gradually along with PB NPs formation. Therefore, electrochemical detection was also used for detecting ZEN. Under the optimum conditions, the logarithm of ZEN concentration showed a good linear relationship with the absorbance from 0.2 to 0.8 ng/mL (R 2 = 0.987) and the differential pulse voltammetry peak current from 0.125 to 0.5 ng/mL (R 2 = 0.993). The limits of detection of colorimetric and electrochemical methods were 0.04 and 0.08 ng/mL, respectively. The recovery rates of ZEN from cornmeal samples were in the range of 80–120%, and relative standard deviations were lower than 10%. The results demonstrated that the dual-signal immunoassay has a high sensitivity for ZEN detection in corn samples.
研究领域:
食品科学
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分析化学
·
毒理学
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Kai Wang,Han Yan,Baoshan He,Lingling Xie,Renli Liu,Min Wei,Huali Jin,Wenjie Ren,Zhiguang Suo,Yiwei Xu
Vomitoxin (DON) residues in grains are of great concern to public health. Herein, a label-free aptasensor was constructed to detect DON distributed in grains. Cerium-based metal-organic framework composite gold nanoparticles ( [email protected] ) were used as substrate materials to facilitate electron transfer and provided more binding sites for DNA. The separation of DON-aptamer (Apt) complex and cDNA was achieved by magnetic separation technique based on magnetic beads (MBs), ensuring the specificity of the aptasensor. Exonuclease III (Exo III)-assisted cDNA cycling process strategy would be triggered when cDNA was separated and introduced to the sensing interface for further signal amplification. Under optimal conditions, the constructed aptasensor presented a wide detection range from 1 × 10 −8 mg·mL −1 to 5 × 10 −4 mg·mL −1 for DON, and the detection limit was 1.79 × 10 −9 mg·mL −1 , including a satisfactory recovery in cornmeal sample spiked with DON. The results showed that the proposed aptasensor had high reliability and promising application potential in detecting DON.
研究领域:
分析化学
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食品科学
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生物传感器
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毒理学
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2024
·Vol. 14
·期 7
·347
·
IF 4.9
Qi Sun,Yuting Zhou,Miaomiao Ma,Fuyan Zhang,Shuang Li,Zhuoer Chen,Yu Fang,Tao Le,Fuguo Xing
This study aimed to develop a novel fluorescent aptasensor for the quantitative detection of zearalenone (ZEN), addressing the limitations of conventional detection techniques in terms of speed, sensitivity, and ease of use. Nitrogen-doped carbon dots (N-CDs) were synthesized via the hydrothermal method, resulting in spherical particles with a diameter of 3.25 nm. These N-CDs demonstrated high water solubility and emitted a bright blue light at 440 nm when excited at 355 nm. The fluorescence of N-CDs was quenched by dispersed gold nanoparticles (AuNPs) through the inner filter effect, while aggregated AuNPs induced by NaCl did not affect the fluorescence of N-CDs. The aptamer could protect AuNPs from NaCl-induced aggregation, but the presence of ZEN weakened this protective effect. Based on this principle, optimal conditions for ZEN detection included 57 mM NaCl, 12.5 nM aptamer concentration, incubation of AuNPs with NaCl for 15 min in Tris-EDTA(TE) buffer, and incubation of aptamer with ZEN and NaCl for 30 min. Under these optimized conditions, the “signal-on” fluorescent aptasensor for ZEN detection showed a linear range of 0.25 to 200 ng/mL with a low detection limit of 0.0875 ng/mL. Furthermore, the developed aptasensor exhibited excellent specificity and could rapidly detect ZEN in corn flour samples or corn oil, achieving satisfactory recovery rates ranging from 84.7% to 108.6%. Therefore, this study presents an economical, convenient, sensitive, and rapid method for accurately quantifying ZEN in cereal products.
研究领域:
分析化学
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食品科学
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纳米技术
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毒理学
✓ 已验证
DOI
PubMed
高影响因子
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