ENVIRONMENT INTERNATIONAL
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2025
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IF 9.7
Qiu E. Yang,Zhenyan Lin,Dehao Gan,Minchun Li,Xuedan Liu,Shungui Zhou,Timothy R. Walsh
Antimicrobial resistance (AMR) and environmental degradation are existential global public health threats. Linking microplastics (MPs) and AMR is particularly concerning as MPs pollution would have significant ramifications on controlling of AMR; however, the effects of MPs on the spread and genetic mechanisms of AMR bacteria remain unclear. Herein, we performed Simonsen end-point conjugation to investigate the impact of four commonly used MPs on transfer of clinically relevant plasmids. The transfer breadth of a representative pA/C_MCR-8 plasmid across bacterial communities was confirmed by the cell sorting and 16S rRNA gene amplicon sequencing. Our study shows that exposure to four commonly found MPs promotes the conjugation rates of four clinically relevant AMR plasmids by up to 200-fold, when compared to the non-exposed group and that the transfer rates are MP concentrations demonstrate a positive correlation with higher transfer rates. Furthermore, we show that MPs induce the expression of plasmid-borne conjugal genes and SOS-linked genes such as recA , lexA , dinB and dinD . High-throughput sequencing of the broad transmission of plasmid pA/C_MCR-8, shows distribution over two main phyla, Pseudomonadota (50.0 %-95.0 %) and Bacillota (0.4 %-2.0 %). These findings definitively link two global health emergencies − AMR and environmental degradation via MPs, and to tackle global AMR, we must also now consider plastic utilisation and waste management.
研究领域:
微生物学
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环境科学
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公共健康
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2014
·Vol. 20
·期 16
·4829-4835
·
IF 5.696
Wenjing Qi,Min Xu,Lei Pang,Zhongyuan Liu,Wei Zhang,Saadat Majeed,Guobao Xu
Graphical An explosive situation : Methods for the electrochemiluminescence (ECL)-based detection of 2,4,6-trinitrotoluene (TNT) through the formation of a TNT–amine complex and through ECL resonance energy transfer have been developed (see figure). The determination of TNT concentration with 3-aminopropyltriethoxysilane (APTES)-modified [Ru(phen) 3 ] 2+ (phen=1,10-phenanthroline)-doped silica nanoparticles (RuSiNPs) down to 1 n M is achieved within 1 min. 2,4,6-Trinitrotoluene (TNT) is a widely used nitroaromatic explosive with significant detrimental effects on the environment and human health. Its detection is of great importance. In this study, both electrochemiluminescence (ECL)-based detection of TNT through the formation of a TNT–amine complex and the detection of TNT through electrochemiluminescence resonance energy transfer (ECRET) are developed for the first time. 3-Aminopropyltriethoxysilane (APTES)-modified [Ru(phen) 3 ] 2+ (phen=1,10-phenanthroline)-doped silica nanoparticles (RuSiNPs) with uniform sizes of (73±3) nm were synthesized. TNT can interact with APTES-modified RuSiNPs through charge transfer from electron-rich amines in the RuSiNPs to the electron-deficient aromatic ring of TNT to form a red TNT–amine complex. The absorption spectrum of this complex overlaps with the ECL spectrum of the APTES-modified RuSiNPs/triethylamine system. As a result, ECL signals of the APTES-modified RuSiNPs/triethylamine system are turned off in the presence of TNT owing to resonance energy transfer from electrochemically excited RuSiNPs to the TNT–amine complex. This ECRET method has been successfully applied for the sensitive determination of TNT with a linear range from 1×10 −9 to 1×10 −6 M with a fast response time within 1 min. The limit of detection is 0.3 n M . The method exhibits good selectivity towards 2,4-dinitrotoluene, p -nitrotoluene, nitrobenzene, phenol, p -quinone, 8-hydroxyquinoline, p -phenylenediamine, K 3 [Fe(CN) 6 ], Fe 3+ , NO 3 − , NO 2 − , Cr 3+ , Fe 2+ , Pb 2+ , SO 3 2− , formaldehyde, oxalate, proline, and glycine.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2025
·
IF 9
Jie Cheng,Yuxin Qiu,Jiahui Chen,Yuqi Gu,Jingwen Wang,Guzhong Chen,Zhiwen Qi,Zhen Song
The inherent merits of natural deep eutectic solvents (NADESs) have endowed them as a class of promising solvents in many applications. In this study, amino acid-based DESs are applied as absorbent for CO 2 capture. A systematic method combining solid–liquid equilibria (SLE) prediction, EHS-impacts (environmental, health, and safety) evaluation of the second components, and experimental validation is employed to rationally identify promising CO 2 absorbents. The melting point and fusion enthalpy of pure amino acid components are predicted by a recently proposed machine learning model, while COSMO-RS is used to predict the SLE of all possible combinations. Five key EHS-related properties of the second components are evaluated via the VEGA platform. From above, eight promising binary systems based on L-lysine and L-proline are screened from the initial pool of 2,500 combinations. The SLE, density, and viscosity are experimentally determined and the CO 2 absorption experiments are finally performed, identifying L-proline: nonanoic acid (1:4) as the most promising eutectic absorbent.
研究领域:
化学工程
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环境科学
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材料科学
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产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
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2022
·
IF 9.224
Jie Cheng,Hao Qin,Hongye Cheng,Zhen Song,Zhiwen Qi,Kai Sundmacher
As traditional methods for retrieving natural vitamin E suffer from harsh operating conditions and poor process performance, seeking more energy-efficient and sustainable alternative approaches is highly desirable. In this work, deep eutectic solvents (DESs) as a class of emerging media are particularly proposed for the extraction of vitamin E (modeled by α-tocopherol) from deodorized distillates under mild operating conditions. To select DESs suitable for this task, a rational screening method is presented which covers the thermodynamic evaluation of individual components and component combinations, the assessment of environment, health, and safety (EHS) impacts of components, and the eutectic behavior estimation of component combinations. Based on experimentally reported DESs from the literature, individual components as well as random combinations of potential hydrogen bond acceptors (HBAs) and hydrogen bond donors (HBDs) are successively prescreened by COSMO-RS predicted thermodynamic properties. Five EHS-related properties of HBDs are assessed by VEGA. The formation of eutectics and the corresponding operating window of different HBA–HBD combinations are estimated by COSMO-RS solid–liquid equilibria prediction. Experiments are finally performed with the top-ranked DESs, which well validates the reliability of the screening method and identifies tetrabutylphosphonium chloride–ethanolamine (2:1) as a suitable DES for the α-tocopherol extraction.
研究领域:
化学工程
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绿色化学
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溶剂科学
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制药技术
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产品关联
DOI
高影响因子
ACS ES&T Water
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2022
·
IF 0
Penghui Du,Wen Liu,Zuomin Rao,Junjian Wang
Peracetic acid (PAA), an alternative disinfectant of chlorine, has drawn increasing attention in the application of wastewater treatment. However, little is known about the influence of water matrices on PAA-induced organic micropollutant (OMP) degradation. Here, we found that the coexisting bromide ions (Br–) in water can trigger the oxidation of OMP during PAA treatment but probably result in higher ecotoxicity. Br– can efficiently decompose PAA with a species-specific rate constant (kPAAH/Br–) of 0.198 ± 0.003 M–1·s–1. The thus generated HOBr led to a significant abatement (31.8–81.3%) of OMPs (17α-ethinylestradiol, sulfamethoxazole, naproxen, and phenol) after a 1 h reaction at pH 7.1. The coexisting H2O2 component in the PAA solution can competitively consume HOBr and inhibit OMP transformation. The OMP degradation in the PAA/Br– process was highly pH-dependent and preferred acidic conditions. Furthermore, a comprehensive model was established to simulate the reaction kinetics of the OMP degradation by the PAA/Br– process with good accuracy. High-performance/electrospray ionization-triple quadrupole mass spectrometry results indicated the generation of various brominated products, with higher model-predicted toxicity than their parent compounds. This work significantly advances the understanding of the role of Br– in OMP oxidation by PAA and alerts the possible environmental health risks.
研究领域:
环境科学
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水处理
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化学工程
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毒理学
✓ 已验证
DOI
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2025
·
IF 6.1
Leisheng Wang,Yifei Fu,Shiwei Xu,Enhong Chen,Shuo Zhang,Dongjie Yang,Youzhao He,Yuanlong Gu,Yong Mao,Fenglai Yuan,Hao Hu
6PPD-quinone (6PPDQ), a derivative of the antioxidant 6PPD used in the rubber industry, has been implicated in environmental and public health risks. To explore its impact on metabolic and cognitive functions, we orally administered 6PPDQ to mice and assessed its effects. Our results show that 6PPDQ induces a dose-dependent increase in body weight, elevated fasting blood glucose, insulin resistance, and other hallmarks of metabolic syndrome. Furthermore, cognitive tests revealed significant impairments in learning and memory in 6PPDQ-treated mice. Transcriptomic and Western blot analyses suggest that 6PPDQ disrupts insulin signaling through inhibition of the PI3K-AKT pathway, leading to metabolic and cognitive dysfunction. Notably, treatment with the PI3K-AKT agonist SC79 partially alleviated these effects. These findings highlight the health risks of 6PPDQ exposure and suggest potential avenues for preventive and therapeutic strategies.
研究领域:
环境毒理学
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神经科学
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代谢性疾病
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信号转导
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药理学
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公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Zhigao Zhao,Xiaowen Dou,Jiaoyang Luo,Meiqi Jin,Jiaan Qin,Changjian Wang,Shihai Yang,Meihua Yang
As a highly toxic and widely used herbicide, atrazine poses a serious threat to food safety as well as overall environmental and human health. Due to complex matrix interference and the difficulty of signal enrichment, there is an urgent need for a convenient, fast, and ultrasensitive method that detects trace atrazine without concern for matrix effects. Here, we provide the first account of a sensitive and rapid suspension probe based on magnetic microspheres used to detect atrazine in herbs. The self-made magnetic beads featured -COOH groups and were used as the carrier to construct immunofluorescent probes. These probes then conjugated with the atrazine antigen through an activated ester method, ultimately binding to the antibody. Homogeneous detection was ensured using flow cytometry and the microflow optical channel along with allophycocyanin-conjugated goat-anti-mouse secondary antibody (APC-IgG-SecAb) as the fluorescent signal. The magnetic suspension probe allowed for high target enrichment and the inherent two-dimensional selective detection of flow cytometry effectively avoided any matrix interference. This method had good linearity across 1.69−23.19 ng mL −1 . The IC 50 and LOD values were 4.81 ng mL −1 and 0.95 ng mL −1 , respectively; the sensitivity was increased three-fold relative to ELISA. After complete optimization, 2 -N- morpholinoeth-anesulfonic acid was used as the coupling solution and maintained good mono-dispersity, stability, and reactivity for the labelled microspheres during the process. The entire experiment was simple, and effectively used reagents; moreover, both the labor required and detection time were greatly reduced. Critically, the strategy presented here greatly reduced interference from complex matrices, and saved preparation for matrix-matched solutions when different herbs were screened. Overall, this strategy was sensitive, rapid, eco-friendly, and labor-saving; collectively, these attributes make it well-suited for on-site screening of atrazine contamination and will allow for increased food safety.
研究领域:
分析化学
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环境科学
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食品科学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Shuli Liu,Jintao Zhou,Xin Yuan,Jun Xiong,Min-Hua Zong,Xiaoling Wu,Wen-Yong Lou
Pesticide residues have raised considerable concern about environmental health and food safety. Despite a great advance in enzymatic sensors for pesticide detection, the intrinsic fragility of native enzyme and possible fake results due to single mode signal have hindered its wide application. Here, a novel dual-mode sensor is reported for organophosphorus pesticide detection by using metal–organic framework (MOF) nanozyme NH 2 -CuBDC as sensing element. The intrinsic peroxidase-mimicking activity and fluorescence property of NH 2 -CuBDC enable both colorimetric and fluorescent detection of chlorpyrifos. Compared with previously reported chlorpyrifos sensors, our sensor exhibits outstanding sensitivity, and the limits of detection (LOD, S/N = 3) in colorimetric and fluorescent modes are 1.57 ng/mL and 2.33 ng/mL, respectively. No obvious interferences from other substances were measured and chlorpyrifos analysis in real samples presented good reliability, showing practical potential. This work is anticipated to provide new insights to develop multifunctional nanozymes and integrated multi-mode sensing platforms.
研究领域:
分析化学
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环境科学
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材料科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Shuang Liu,Miao Yu,Xincen Xie,Yiran Ru,Shaoguo Ru
Carbofuran , a carbamate pesticide , is widely used in developing countries to manage insect pests. Studies have found that carbofuran posed potential risks for the neurotransmitter systems of non-target species, we speculated that these disruptive effects on the neurotransmitter systems could trigger anxiety-like behaviors. In this study, female zebrafish were exposed to environmental levels (5, 50, and 500 μg/L) of carbofuran for 48 h to evaluate the effects of carbofuran on anxiety-like behaviors. Results showed that zebrafish exhibited more anxiety-like behaviors which proved by the observed higher bottom trend and more erratic movements in the novel tank after carbofuran treatment. In order to elucidate the underlying molecular mechanisms of carbofuran-induced anxiety-promoting effects, we measured the levels of neurotransmitters, precursors, and major metabolites, along with the level of gene expression and the enzyme activities involved in neurotransmitter synthesis and metabolism. The results demonstrated that acute carbofuran exposure stimulated the mRNA expression and enzyme activity of tyrosine hydroxylase, which sequentially induced the increased levels of dopamine and norepinephrine . Tyrosine hydroxylase inhibitor relieved the anxiety-related changes induced by carbofuran, confirming the overactive tyrosine hydroxylase-mediated accumulation of dopamine and norepinephrine in the brain was one of the main reasons for carbofuran-induced anxiety-like behaviors in the female zebrafish. Overall, our study indicated the environmental health risks of carbamate pesticide in inducing neurobehavioral disorders and provided novel insights into the investigation of the relevant underlying mechanisms.
研究领域:
环境毒理学
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神经科学
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水生毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICOLOGY
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2022
·
IF 4.571
Jielin Tang,Jiayi Li,Qinyu Zhou,Guzailinuer Kuerban,Jinyan Qin,Hongdan Zhang,Rongli Sun,Lihong Yin,Yuepu Pu,Juan Zhang
As a new type of flame retardant , Organic Phosphate Flame Retardant has been widely used worldwide. The purpose of our research is to determine the neurotoxicity of Tris (1,3-dichloroisopropyl) phosphate (TDCPP) to Caenorhabditis elegans and its mechanism. L1 larvae wild-type C. elegans were exposed to different concentrations of TDCPP, and the effects on motor behavior (head thrashes, body bends, pumping times, chemotaxis index), ROS levels, and p38MAPK signaling pathway-related gene expression levels were measured. Three transgenic nematode strains, BZ555, DA1240, and EG1285, were also used to study the effects of TDCPP on nematode dopamine neurons, glutamate neurons, and GABA neurons. The results showed that TDCPP can inhibit the head thrashes and body bends of the nematode, reduce dopamine production, increase the level of ROS in the body, and affect the expression of genes related to the p38MAPK signaling pathway. We next employed ROS production and motor behavior as toxicity assessment endpoints to determine the involvement of p38 MAPK signaling in the regulation of response to TDCPP. The results showed that the nematodes with low expression of pmk-1 were less sensitive to the TDCPP. It was suggested that TDCPP had neurotoxicity and regulated neurotoxicity to C. elegans by activating the p38-MAPK signaling pathway. The research in this article provides important information for revealing the environmental health risks of organophosphorus flame retardants and their toxic mechanism of action.
研究领域:
毒理学
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环境健康
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分子生物学
·
神经科学
✓ 已验证
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2019
·
IF 3.775
Hua Chen,Chunlei Zhu,Feng Chen,Jingjing Xu,Xiuting Jiang,Zeyu Wu,Xiaowei Ding,Gao-Chao Fan,Yizhong Shen,Yingwang Ye
Fluoroquinolone antibiotics (FQs), a new class of pollutants that seriously threaten human health through environmental and food residues, have aroused wide public concern. However, little attention has been paid to the potential toxicity of FQs' metal complex. Here, we firstly explore the proof-of-concept study of FQs' metal complex to bind bovine serum albumin (BSA) using systematical spectroscopic approaches. In detail, we have found that the complex of Al 3+ with gatifloxacin (Al(III)-GFLX complex) can effectively bind to BSA via electrostatic interaction in PBS buffer (pH = 7.4, 1×), resulting in the formation of Al(III)-GFLX-BSA complex. The negative value of Δ G shows that the binding of Al(III)-GFLX complex to BSA is a spontaneous process. Circular dichroism spectra verify that Al(III)-GFLX complex effectively triggers the conformation changes of BSA's secondary structure. It has been proved that the interaction of small molecule with serum albumin has a significant effect on their in vivo biological effects such as absorption, distribution, metabolism, and excretion, and etc. Therefore, the results of this paper may offer a valuable theoretical basis for establishing safety standards of FQs' metal complex to ensure food and environmental health .
研究领域:
环境科学
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毒理学
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药物化学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Yue Wang,Xiufang Liang,Nicholas Andrikopoulos,Huayuan Tang,Fei He,Xiang Yin,Yuhuan Li,Feng Ding,Guotao Peng,Monika Mortimer,Pu Chun Ke
Understanding the environmental health and safety of nanomaterials (NanoEHS) is essential for the sustained development of nanotechnology. Although extensive research over the past two decades has elucidated the phenomena, mechanisms, and implications of nanomaterials in cellular and organismal models, the active remediation of the adverse biological and environmental effects of nanomaterials remains largely unexplored. Inspired by recent developments in functional amyloids for biomedical and environmental engineering, this work shows their new utility as metallothionein mimics in the strategically important area of NanoEHS. Specifically, metal ions released from CuO and ZnO nanoparticles are sequestered through cysteine coordination and electrostatic interactions with beta-lactoglobulin (bLg) amyloid, as revealed by inductively coupled plasma mass spectrometry and molecular dynamics simulations. The toxicity of the metal oxide nanoparticles is subsequently mitigated by functional amyloids, as validated by cell viability and apoptosis assays in vitro and murine survival and biomarker assays in vivo. As bLg amyloid fibrils can be readily produced from whey in large quantities at a low cost, the study offers a crucial strategy for remediating the biological and environmental footprints of transition metal oxide nanomaterials.
研究领域:
纳米技术
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环境科学
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生物医学工程
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毒理学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）