SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Mengyao Wang,Honglin Yang,Yue Wu,Zhifeng Fu
A dual sites affinity protocol was developed for fluorescent analysis of Staphylococcus aureus ( S. aureus ) by employing daptomycin and immunoglobulin G (IgG) as the recognition elements . Pig IgG immobilized on microplate was employed as the first recognition element to capture S. aureus owing to the fact that the Fc segment of mammal IgG can selectively bind with protein A on the surface of the target bacteria. Meanwhile, fluorescein isothiocyanate-conjugated daptomycin was employed as the second recognition element as well as the signal tracer for the target bacteria utilizing the binding capability of daptomycin to Gram-positive bacteria. S. aureus can be analyzed within a concentration range of 5.0 × 10 3 –5.0 × 10 8 CFU mL −1 with a detection limit of 3.6 × 10 3 CFU mL −1 . The analytical process can be accomplished within 1.5 h by using a pre-coated microplate. The dual sites affinity protocol can exclude the interference led by Gram-negative bacteria and other common Gram-positive bacteria. We have successfully applied it to analyze S. aureus in spiked lake water and physiological saline injection samples, and the recovery values ranged from 88.0% to 120.0%. The results demonstrate its application potential for environmental sanitation and drug safety control.
研究领域:
微生物学
·
分析化学
·
环境科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Ran Wang,Xinyu Li,Guangjiang Yu,Liqun Ren,Jinxia Liu,Tifeng Jiao
In recent years, respiratory infectious diseases have caused severe damage to human life and health as well as social development. New types of filtration and antibacterial materials have attracted widespread attention. In this work, chitosan quaternary ammonium salt (HACC) was used as antibacterial agents to prepare silk fibroin (SF)-based composite film materials with good antibacterial and filtration properties by electrospinning technology. The prepared composite film has good cytocompatibility and is free of irritation and allergenicity. In addition, the mask constructed with SF-based electrospun film as the filter layer material has an inhibition rate of more than 90 % against Escherichia coli and Staphylococcus epidermidis . Furthermore, the obtained electrospun film shows a good antibacterial effect on Staphylococcus epidermidis and Escherichia coli . This work provides new ideas for the preparation of new materials and their applications in mask filtration and antibacterial fields.
研究领域:
材料科学
·
生物医学工程
·
环境卫生
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
You Long,Yuting Wang,Gan Zhang,Bei Gan,Qiang Yu,Jianhua Xie,Xinyi Zang,Yi Chen
Enniatin and Alternaria mycotoxins have presented adverse effects to food safety and human health, and an accurate, simultaneous analytical method for these mycotoxins was promising. This study reported the synthesis of a magnetic covalent organic framework (NH 2 -Fe 3 O 4 @TpBD-COF) for efficient extraction of seven mycotoxins from wheat, coupled to UPLC-MS/MS for simultaneous quantification. The magnetic solid phase extraction (MSPE) parameters, including adsorbent dose (10 mg), vortex duration (5 min), and extraction/elution solvents (ACN/MeOH, 6/4, v /v), were systematically optimized. Method validation revealed ultra-low limits of quantification (LOQs:0.03 to 0.5 μg kg −1 ), matrix effects (ME<8.49 %), recovery rates(81.3 %–109 %), and precision (RSD < 9.70). The adsorbent maintained satisfactory performance through 8 reuse cycles. Density functional theory (DFT) calculation indicated exergonic adsorption, primarily driven by hydrogen bonding, van der Waals forces, and hydrophobic interactions. The integrated method offers a sensitive and accurate approach for emerging mycotoxin analysis in wheat, potentially mitigating associated health risks.
研究领域:
食品安全
·
分析化学
·
真菌毒素学
·
材料科学
·
环境卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL MONITORING AND ASSESSMENT
·
2024
·Vol. 196
·期 12
·1152
·
IF 2.9
Alamgir Aamir,Fatima Noor
In the present study, piped water samples from Gulshan-e-Iqbal Town, Karachi, were evaluated for their Health Risk Assessment (HRA) and Water Quality Index (WQI). For this, different physicochemical and microbiological parameters were analyzed, and the results were evaluated according to the guidelines established by the WHO (2011) and the National Standards for Drinking Water Quality (NSDWQ) (2008). With the exception of sulphate, all physicochemical parameters were well within the guideline values. The mean concentrations of the metals in the samples were in the following order: Ca 2+ > Mg 2+ > Zn > Fe > Pb > Ni > Cr > As. More than 70% of the samples tested for Total Coliform Count (TCC), Total Faecal Coliform Count (TFC), Total Feacal Streptococci (TFS), and Total Aerobic Count (TAC) were feacal contaminated. E. coli was also isolated in almost 84.61% of the tested piped water samples. Results from the WQI revealed that 95.6% of samples had good physico-chemical characteristics, and 26% of the piped water samples had good microbiological quality. The WQI readings of all the samples for metals showed that they were unfit for human consumption. The mean Chronic Daily Intake (CDI) and Hazard Quotient (HQ) values, respectively, were in the following order: Zn > Fe > Pb > Ni > Cr > As, and Pb > As > Ni > Cr > Zn > Fe. All HQ values for As and Pb were greater than 1, and 50% of piped water samples had an HQ value for Ni that was greater than 1, indicating that people may suffer serious health issues as a result. All of the piped water samples except the area of block 13 D (S-5), had HQ values of less than 1 in relation to iron, but Zn HQ values of less than 1 indicated only mild health problems. Discharge of untreated sewage and cross-contamination are all potential sources of contamination that could result in diseases that are harmful to the public’s health. Water monitoring and management projects should be implemented in order to improve pipeline infrastructure and reduce sewage leakages.
研究领域:
环境卫生
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水质管理
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公共卫生
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环境科学
·
毒理学
✓ 已验证
DOI
高影响因子
Environment & Health
·
2023
·
IF 0
Wei Wei,Bingwei Yang,Xiangyu Zhu,Xuting Liu,Erqun Song,Yang Song
When nanoparticles (NPs) enter a physiological environment, they tend to adsorb proteins to form a so-called corona. A comprehensive understanding of the effect of protein corona on NPs’ toxicity is required. Our previous study indicated that silica nanoparticles (SiO2 NPs) exposure with different routes resulted in distinct brain damage; however, an exact molecular mechanism of protein corona on the regulation of SiO2 NPs-induced damages needs further investigation. SiO2 NPs exposure via intravenous injection may encounter a protein-rich bio-matrix, which drives the adsorption of serum protein on their surface to form a stable SiO2 NPs@serum complex. On the contrary, SiO2 NPs exposure via intranasal instillation remained their original feature, due to a protein infertile environment of cerebrospinal fluid. Apparently, surface adsorption of proteinaceous substances altered inherent toxic behavior of SiO2 NPs. In addition, glycogen synthase kinase 3 beta (GSK3β) phosphorylate was found at different residues, which play an essential role in orchestrating apoptosis and autophagy threshold. Route-dependent corona formation determined GSK3β phosphorylation status and ultimately the toxic behavior of SiO2 NPs. This work presented the evidence of bio-corona on the regulation of SiO2 NPs-induced toxicity, which can be used to guide risk assessment of environmental NPs.
研究领域:
纳米毒理学
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神经毒理学
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环境卫生
·
生物化学
·
分子生物学
✓ 已验证
DOI
Journal of Hazardous Materials Advances
·
2025
·
IF 7.7
Yanjun Liu,Zefei Yang,Jingyao Chen,Huiyuan Yang,Yujia He,Zhengju Lv,Junbo Wang,Jianbing Wang
Landfills are significant sources of fugitive benzene release, posing a threat to the health of occupational populations. In this study, long-term monitoring of both external and internal benzene occupational exposure was conducted at a municipal solid waste (MSW) landfill site. The annual average concentration of benzene exposure in the landfill was 0.78 ± 1.08 μg/m³, with a notable increase in autumn (1.40 ± 5.29 μg/m³). The internal biomarker level (urinary t, t-muconic acid) significantly increased post-shift for the occupational population (6.65 ± 50.75 mg/g cr) compared to pre-shift (5.32 ± 31.62 mg/g cr, p < 0.05), exceeding the American Conference of Government Industrial Hygienists (ACGIH) limit by 13 times (0.5 mg/g cr). Machine learning models, particularly the Support Vector Regression (SVR) algorithm, outperformed traditional methods (e.g., Michaelis-Menten) in predicting internal exposure (R² = 0.989, root mean square error = 0.085). Using the SVR model, the predicted internal benzene level under the occupational exposure limit (1.7 mg/m³) was 1.65 mg/g cr, exceeding the ACGIH limit by three-fold. These findings provide a novel framework for benzene exposure risk assessment and inform targeted landfill management strategies.
研究领域:
环境卫生
·
毒理学
·
机器学习
✓ 已验证
产品关联
DOI
高影响因子
TOXICOLOGY
·
2025
·
IF 4.6
Ziyan Liu,Jingyu Wang,Wei Zhang,Huiwen Kang,Danyang Huang,Ziyan Wang,Guangyu Jiang,Ai Gao
The continuous accumulation of senescent hematopoietic stem progenitors (HSPCs) contributes to hematopoietic damage. Benzene is a confirmed human carcinogen, and its damage to HSPCs is a key event in benzene poisoning. However, whether the environmental dose of benzene is involved in HSPC damage by inducing cellular senescence has not been reported. Here, male C57BL/6 J mice were exposed to benzene vapour for 12 weeks (0, 10, 50 ppm), and mouse hematopoietic progenitor cell line FDC-P1 was exposed to benzene metabolite 1,4-BQ for 24 h (0, 5, 10 μM). In vivo and in vitro models combined with single-cell RNA sequencing have shown that benzene and its metabolites caused senescence in whole bone marrow and hematopoietic progenitor cells. Proteomics showed that benzene exposure significantly up-regulated interferon regulatory factor 4 (Irf4) in the bone marrow. Irf4 inhibition alleviated cellular senescence and hematopoietic damage, suggesting that Irf4 is a key molecule in benzene-induced hematopoietic cell senescence. Mechanistically, benzene caused a decrease in branched-chain amino acids (BCAAs) in whole bone marrow, hematopoietic progenitor cells, and plasma, and an increase in the BCAA catabolic enzyme Bcat1, mitochondrial ROS, and Bckdh activity. Irf4 inhibition down-regulated Bcat1, alleviated mitochondrial oxidative stress-dependent Bckdh abnormality, and up-regulated BCAAs. BCAA supplementation effectively alleviated benzene-induced cellular senescence and hematopoietic damage. In conclusion, the study identified that Irf4 triggered benzene-induced hematopoietic progenitor cell senescence and hematopoietic damage by mitochondrial injury-induced excessive BCAA catabolism. This study provides new ideas on the molecular mechanism of benzene-induced hematopoietic damage from the perspective of metabolism and senescence.
研究领域:
毒理学
·
血液学
·
分子生物学
·
环境卫生
·
代谢
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Lulin Hu,Weiyan Yu,Yi Yang,Jingcheng Hao,Lu Xu
As a class of very common and important industrial liquid materials, metalworking fluids (MWFs) are easy to be vaporized into the air and cause pulmonary toxic effects. The inhaled MWF aerosols should first interact with the pulmonary surfactant (PS) film that plays an essential role in maintaining the normal respiratory mechanics and pulmonary immunology in a human body. Here, to probe any potential adverse impacts of airborne MWFs on the biophysical and physiological functions of PS film that may help achieve a deep and comprehensive understanding of the pulmonary toxicology of MWFs, a systematic study on the interaction between an animal-derived natural PS ( i.e. , Calsurf) film and the aerosols of water-based MWFs and their different constituents is conducted using constrained drop surfactometry (CDS) capable of closely simulating normal tidal breathing and lung-related physiological conditions in vitro . It was found that the airborne MWFs can induce strong PS inhibitions once their accumulated amount in the environment attains 0.2 mg/cm 3 . And their inhibitory effects are demonstrated to mainly originate from the surfactant additives such as polyethylene glycol monooleate (PEGMO) that can desorb PS film from the air/water interface via competitive interfacial adsorption, although it has normally been regarded as an eco-friendly commercial reagent.
研究领域:
工业毒理学
·
肺生物学
·
生物物理学
·
环境卫生
·
材料科学
·
职业健康
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2025
·
IF 8
Yuxin Lin,Luyao Wang,Sining Meng,Jianjia Liu,Xinyu Li,Wenjuan Wang,Miaoyang Hu,Xi Wang,Weixia Duan,Shangcheng Xu,Jun Wang,Rong Gao
Bisphenol F (BPF), one of the major substitutes for bisphenol A (BPA), is increasingly used in pharmaceuticals, food packaging, and chemical manufacturing. Emerging evidence suggests its potential neuropsychiatric risks. However, the underlying mechanisms remain poorly characterized. Thus, the present study was performed to investigate the roles of the kynurenine (KYN) pathway in BPF induced anxiety- and depression-like behaviors, especially the keys roles of the toxic metabolite 3-hydroxykynurenine (3−HK). The mice were administrated with BPF (10, 40, 160 μg/kg/day) for 30 consecutive days, and the behaviors as well as the changes of the KYN pathway were examined. Noteworthily, even low-dose of BPF exposure (40 μg/kg/day) elicited pronounced anxiety- and depression-like behaviors, molecularly shown as the synaptic marker proteins (PSD95, SYN) and dendritic spine proteins (Drebrin) loss in the prefrontal cortex and hippocampus, along with impaired synaptic connection. The UHPLC-MS/MS metabolomic profiling revealed the aberrant metabolism of the KYN pathway, as shown by the elevated neurotoxic metabolite 3-HK and decreased neuroprotective metabolite kynurenic acid (KynA). Mechanistic analyses demonstrated that the 3-HK rate-limiting enzyme KMO was upregulated while the KynA rate-limiting enzyme KAT2 was downregulated in mouse brain. Notably, pharmalogical inhibition of KMO (GSK180) or blockage kynurenine generation with the TDO2 inhibitor (680C91) substantially ameliorated the BPF induced anxiety- and depression-like behaviors and synaptic impairments. Collectively, the present study unravels a previously unrecognized the BPF induced anxiety- and depression-like behaviors in mice via KMO and 3-HK increase. Therefore, targeting the KMO may provide novel interventive strategy for BPF induced neurobehavioral disorders.
研究领域:
神经科学
·
毒理学
·
药理学
·
分子生物学
·
行为科学
·
代谢组学
·
环境卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yuan Yang,Xiaoli Wu,Qiuyi Yan,Hongling Zhou,Yurong Deng,Chengbin Zheng
Furans, recognized as carcinogenic contaminants generated during thermal food processing, pose significant food safety concerns, necessitating sensitive and cost-effective detection methods. Traditional GC-FID/MS methods are often constrained by high costs and complex instrumentation. Herein, a point discharge molecular emission spectrometer (PD-MES) was developed as a highly sensitive GC detector, specifically by monitoring CN molecular emission at 388.3 nm for the analysis of furans in food samples. Furans were extracted using headspace solid-phase microextraction (HS-SPME) prior to detection. Compared to conventional carbon atomic emission at 193.0 nm, the CN-based method demonstrated up to a 211.42-fold improvement in sensitivity and achieved detection limits of 0.02–0.08 ng/g with excellent precision (RSD < 4.9 %). Practical validation across ten diverse food matrices yielded satisfactory recoveries (83.4 %–114.5 %). This system offers a robust, highly sensitive, reliable, and economical alternative for the routine monitoring of furan compounds in foods, particularly in resource-limited settings.
研究领域:
食品安全
·
分析化学
·
光谱学
·
环境卫生
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2025
·
IF 11.3
Siyi Wang,Min Nian,Yu Ma,Xiaojia Chen,Xiaotong Ji,Changzhi Shi,Xing Chen,Mingliang Fang
Thyroid disorders are among the most prevalent endocrine conditions worldwide, exhibiting a rising incidence and disproportionately affecting women. In this study, we hypothesized that cosmetics may contain previously unidentified thyroid-disrupting chemicals. To evaluate this possibility, we compiled a comprehensive data set of cosmetic ingredients and developed a random forest regression-based machine learning model to predict their potential to disrupt thyroid hormone receptor β (TRβ), a critical regulator of thyroid function. From the top 40 compounds ranked by the model, 12 frequently used cosmetic ingredients were selected for experimental validation. Of these, six demonstrated measurable binding affinity toward TRβ. Notably, Vat Blue 6 (VB6), a colorant utilized in cosmetic formulations, exhibited structural characteristics potentially mimicking thyroid hormones and displayed potent TRβ binding with an affinity (Kd) as low as 0.7 μM. Subsequent in vitro assays and in vivo experiments in mice confirmed VB6’s thyroid-disrupting effects, evidenced by dose-dependent reductions in serum thyroid hormone concentrations and morphological alterations of thyroid tissue. This study highlights the efficacy of machine learning approaches in rapidly screening large chemical inventories to identify potential thyroid disruptors and underscores the critical need for further toxicological assessment of cosmetic ingredients, particularly considering their frequent and prolonged exposure among female populations.
研究领域:
毒理学
·
内分泌学
·
机器学习
·
药理学
·
计算生物学
·
环境卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Shuofeng Li,Qianqian Wang,Lin Hao,Zhi Li,Chun Wang,Qiuhua Wu
A sensitive method for monitoring fluoroquinolones (FQs) is urgently needed to protect public and environmental health. Herein, a novel porous organic cage-based hyper crosslinked polymer with amino functional groups (named as Cage-HCP1-NH 2 ) was prepared through a facile synthetic method. Due to the unique host-guest interaction, F-π, π-π, F O, and hydrogen bond interactions, the Cage-HCP1-NH 2 exhibited high adsorption performance for FQs with the adsorption capacity up to 408.3 mg g −1 . Then, Cage-HCP1-NH 2 based solid phase extraction was combined with high performance liquid chromatography to sensitively quantify trace amounts of FQs in chicken and pork samples. In the investigated FQs concentration range, the proposed method had excellent linearity (R 2 > 0.9960), high precision (relative standard deviation, RSD < 10 %) and low detection limit (0.30–0.6 ng g −1 ). This study not only presents a viable method for sensitive detection of FQ, but also offers an innovative strategy for the creation of novel cage-based materials and promotes their application in more fields.
研究领域:
分析化学
·
高分子科学
·
食品安全
·
环境卫生
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）