SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2015
·
IF 2.353
Shuai Zhou,Ze-Quan Zhou,Xuan-Xuan Zhao,Yu-Hao Xiao,Gang Xi,Jin-Ting Liu,Bao-Xiang Zhao
We have developed a novel fluorescent chemosensor ( DAM ) based on dansyl and morpholine units for the detection of mercury ion with excellent selectivity and sensitivity. In the presence of Hg 2+ in a mixture solution of HEPES buffer (pH 7.5, 20 mM) and MeCN (2/8, v/v) at room temperature, the fluorescence of DAM was almost completely quenched from green to colorless with fast response time. Moreover, DAM also showed its excellent anti-interference capability even in the presence of large amount of interfering ions. It is worth noting that DAM could be used to detect Hg 2+ specifically in the Yellow River samples, which significantly implied the potential applications of DAM in the complicated environment samples.
研究领域:
分析化学
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 18
·3797
·
IF 4.6
Bin Lin,Fen Wang,Hongliang Wang,Xinsheng Huang,Xueqin Liu,Xuechun Wang,Chihua Wang,Yan Xing,Chunqing Dai,Yi Zheng
Theophylline represents a significant public health challenge due to its dual acute and chronic toxicity resulting from therapeutic, environmental, and dietary exposures. Effective monitoring across the bio-environmental–food triad requires analytical methods that are highly sensitive, universally applicable, and capable of overcoming complex matrix interferences. This study introduces a flat membrane-based liquid-phase microextraction (LPME) technique combined with LC–MS/MS for the determination of theophylline in diverse matrices. The method eliminates the need for specialized adsorbents or equipment, offering a simple and cost-effective solution for high-throughput sample clean-up. Under optimized conditions, the method demonstrated exceptional sensitivity (LOD: 0.2 ng mL−1) and a wide linear range (0.01–10 μg mL−1). It was successfully applied to plasma, urine, hospital sewage, and green tea, providing accurate (recoveries of 86.7–111.3%) and reproducible (RSD < 10%) results across all matrices. This unified and robust approach effectively addresses matrix interferences and provides a reliable tool for the monitoring and risk assessment of theophylline across multiple domains.
研究领域:
药物代谢
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分析化学
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环境毒理学
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临床药理学
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食品安全
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生物监测
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色谱质谱技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Jihua Wei,Zichun Song,Jiuying Cui,Yuanxun Gong,Qianli Tang,Kai Zhang,Xinlei Song,Xianjiu Liao
In this study, we introduce an electrochemiluminescence (ECL) sensing platform based on the "Entropy-driven triggered T7 amplification-CRISPR/Cas13a system" (EDT-Cas). This platform combines a programmable entropy-driven cycling strategy, T7 RNA polymerase, and the CRISPR/Cas13a system to amplify the determination of the SARS-CoV-2 RdRp gene. The Ti 3 C 2 T x -compliant ECL signaling molecule offers unique benefits when used with the ECL sensing platform to increase the assay sensitivity and the electrode surface modifiability. To obtain the T7 promoter, the SARS-CoV-2 RdRp gene may first initiate an entropy-driven cyclic amplification response. Then, after recognizing the T7 promoter sequence on the newly created dsDNA, T7 RNA polymerase starts transcription, resulting in the production of many single-stranded RNAs (ssRNAs), which in turn trigger the action of CRISPR/Cas13a. Finally, Cas13a/crRNA identifies the transcribed ssRNA. When it cleaves the ssRNA, many DNA reporter probes carrying -U-U- are cleaved on the electrode surface, increasing the ECL signal and allowing for the rapid and highly sensitive detection of SARS-CoV-2. With a detection limit of 7.39 aM, our method enables us to locate the SARS-CoV-2 RdRp gene in clinical samples. The detection method also demonstrates excellent repeatability and stability. The SARS-CoV-2 RdRp gene was discovered using the "Entropy-driven triggered T7 amplification-CRISPR/Cas13a system" (EDT-Cas). The developed ECL test had excellent recoveries in pharyngeal swabs and environmental samples. It is anticipated to offer an early clinical diagnosis of SARS-CoV-2 and further control the spread of the pandemic.
研究领域:
生物技术
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分子生物学
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病毒学
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诊断技术
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Production Processing and Nutrition
·
2024
·Vol. 6
·期 1
·1-10
·
IF 4
Liu Hao,Chen Jianan,Dong Meng,Pang Jie,Qin Lei,Huang Xuhui
The aim of this research was to determine the residues of dioxins and dioxin-like polychlorinated biphenyls in cultured eel and find out the source of pollution and the distribution pattern of eels. One hundred samples of eel, 4 fodder samples, and 12 environmental samples (water, plants, and soil) were collected from 4 cities and counties in Jiangxi Province, China. The contents of 17 kinds of dioxins (PCDD/Fs) and 12 kinds of dioxin-like polychlorinated biphenyls (dl-PCBs) were determined by high-resolution gas chromatography-tandem mass spectrometry, and the exposure risk was evaluated by using risk index. The total toxicity equivalence quantity (TEQ) of dioxin and dioxin-like polychlorinated biphenyls in eel and fodder samples collected in the study area were 0.65 ± 0.31 pg/g and 0.10 ± 0.02 pg/g, respectively. Dl-PCBs were the main dioxin pollution in eel and fodder samples. 2,3',4,4',5-pentachlorodiphenyl and 2,3,3',4,4'-pentachlorobiphenyl were the main contributing monomers. The environmental samples were mainly polluted by polychlorinated dibenzo-p-dioxins (PCDDs), with the main contributing monomer being Octachlorodibenzodioxins (OCDD), while 3,3',4,4',5-pentachlorobiphenyl and 2,3,4,7,8-pentachlorodibenzofuran were the main toxic compounds in eel and fodder. The dioxin pollution of eels cultured in Jiangxi Province was mainly from fodder polluted via dl-PCBs. The meat segment VI (tail) exhibited a strong enrichment effect of polychlorinated benzofurans (PCDFs). It should be the key part for dioxins and dioxin-like polychlorinated biphenyls assessment. Further, the results were helpful to improve the edible safety of eel products and the efficiency of the risk assessment of dioxins and dioxin-like polychlorinated biphenyls in fish. Graphical
研究领域:
环境科学
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毒理学
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食品安全
·
分析化学
·
水产养殖
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）