In this study, thermoresponsive and mussel-inspired polypeptides were synthesized using ring-opening polymerization of α-amino acid derivatives of N-carboxyanhydride (NCA). The tissue adhesive properties of these polypeptides were evaluated using in vitro adhesive strength tests on porcine skin and bone. The results indicated that the species of the functional polypeptide side groups and the adhesive temperature have a significant influence on the adhesion strength. The maximum of the lap-shear adhesion strength on porcine skin was 101.2 kPa, and the maximum of tensile adhesion strength on bone was 603 kPa. The in vivo antibleeding activity and tissue adhesive ability were also evaluated using a rat model. These polypeptides exhibited superior hemostatic properties and healing effects in the skin incision and osteotomy gap, and the skin incision healing and osteotomy gap remodeling were completed in all rats after 2–9 weeks. These polypeptides are expected to be good candidates for surgical tissue adhesives, tissue engineering materials, and antibleeding materials, etc.
Photoinduced reversible-deactivation radical polymerization (RDRP) has attracted great interest in virtue to their unique inherent advantages, such as low activation energy, environmentally benign reaction conditions and precision synthesis of designable functional polymeric materials. In this work, a facile and efficient strategy for preparing block copolymers was developed by the combination of near-infrared (NIR) light-controlled in-situ bromine-iodine transformation reversible-deactivation radical polymerization (BIT-RDRP) and purple LED light induced ring-opening polymerization (ROP). Herein, the macroinitiator was first synthesized via NIR light-controlled BIT-RDRP by using a bifunctional 2-hydroxyethyl 2-bromo-2-phenylacetate (EBP-OH) as the initiator at room temperature. Subsequently the block copolymers with narrow dispersity (low up to 1.06) were prepared by combination of purple LED light induced ROP through “two step” or “one pot” process. Importantly, the “one pot” strategy can greatly simplify the operation process and improve the synthesis efficiency, which will facilitate the batch preparation of block copolymers.
危险品化学品经营许可证（带存储）