RSC Advances
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2017
·Vol. 7
·期 63
·40020-40027
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IF 3.108
Rongrong Chen,Yakun Li,Liang Tang,Haocheng Yang,Zetong Lu,Jun Wang,Lianhe Liu,Kazunobu Takahashi
Marine fouling organisms have caused inconvenience to humans for a long time owing to their high vitality and great destructiveness. Self-polishing antifouling coatings are considered to be among the most effective antifouling technologies. In this study, zinc-based acrylate copolymers (ZnPs) were designed and synthesized using a bifunctional zinc acrylate monomer (ZnM) as a new self-polishing monomer, and three acrylate monomers (namely, methyl methacrylate, ethyl acrylate and 2-methoxyethyl acrylate) were used as comonomers. ZnPs that contained the new ZnM were characterized by Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy and gel permeation chromatography. Different antifouling coatings were prepared using the previously mentioned ZnPs as the matrix material, and their erosion properties were investigated using a lab rotor test. A field test of the prepared coatings at various geographical locations showed their excellent antifouling performance as they inhibited the settlement of barnacles in both the South China Sea for 9 months and in the Yellow Sea for at least 15 months. The results of this study highlight that the biocidal ZnP-based coatings are highly promising candidates for marine antifouling applications.
研究领域:
材料科学
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海洋生物学
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化学工程
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2021
·
IF 6.558
Haochen Qi,Xiaofan Huang,Jayne Wu,Jian Zhang,Fei Wang,Hao Qu,Lei Zheng
Copper ion (Cu 2+ ) is an important cofactor for many enzymes in human body. Either excessive or deficient Cu 2+ in the body may cause serious dysfunctions and diseases. So sensitive determination of Cu 2+ in environmental samples is of more significance for evaluation and control of Cu 2+ intake. Based on a low-cost gold-plated coplanar electrode array, a disposable aptasensor is developed with an ultra-sensitive indicator of interfacial capacitance. Modified with a specially isolated DNA aptamer for Cu 2+ , this sensor achieves a high selectivity of 1207: 1 against non-target ions. To realize real-time response, alternating-current electrothermal effect is integrated into the capacitance measuring process to efficiently enrich the trace Cu 2+ . This sensor reaches a limit of detection of 2.97 fM, with a linear range from 5.0 fM to 50 pM. The response time is only 15 s, which can meet the real-time detection requirement. On-site test of practical samples is also realized using the disposable sensor combined with a handheld inductance/capacitance/resistance meter. This sensor with its portable test system provides a cost-efficient solution for on-site, real-time and sensitive detection of Cu 2+ , showing great application value in environment monitoring.
研究领域:
环境科学
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分析化学
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生物传感器
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2024
·
IF 8
Jiali Qiao,Jingyu Jia,Weipan Peng,Minghui Lu,Yaru Li,Shuli Man,Shengying Ye,Long Ma
Early detection and control of foodborne diseases are crucial for ensuring food safety and consumer health. Therefore, there is a need for faster and more sensitive detection methods. We developed a rapid, portable and visible lateral flow biosensor (LFB) for foodborne pathogenic bacteria detection powered by a Pyrococcus furiosus Argonaute nuclease ( Pf Ago), named as Pf Ago-LFB. LAMP (Loop-mediated isothermal amplification) was used to amplify the target nucleic acid . By using Pf Ago as the “lighting up” technology, we can overcome the limitations of false-positive results and lack of effective endpoint monitoring in LAMP, greatly enhancing its applicability. It turned out that Pf Ago-LFB was able to provide yes-or-no (positive or negative) results with a limit of detection (LOD) as low as 1 CFU/mL for S. typhimurium and the detection range was between 10 0 to 10 8 CFU/mL. It was able to secure a sample-to-answer test for contaminated food samples in less than 45 min and demonstrated satisfactory selectivity. In summary, we designed a Pf Ago-LFB that rendered a novel, rapid, accurate, easy-to-deploy, on-site and yes or no detection of S. typhimurium , which could be generalized for building a “one-for-all” biosensing platform.
研究领域:
食品安全
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生物技术
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分子生物学
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病原体检测
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DOI
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Guangchun Song,Junjie Zhang,Huixian Huang,Xin Wang,Xiaoyun He,Yunbo Luo,Jin-cheng Li,Kunlun Huang,Nan Cheng
Rapid detection of pesticide residues based on enzyme mimics has recently attracted much interest. However, most nanozymes have low activity. Herein, a “single-atom Ce-N-C nanozyme” (SACe-N-C nanozyme) was rationally devised and verified to mimic peroxidase (POD-like) with superior activity. Based on its high POD-like activities and cascaded catalytic reactions with acetylcholinesterase (AChE), we constructed a bioactive paper for the detection of pesticide residues, which offered a portable approach to monitor fruits and vegetables within 30 min. More importantly, a 3D printed platform was integrated on the basis of SACe-N-C bioactive paper to achieve on-site portable testing of omethoate, methamidophos, carbofuran, and carbosulfan, showing limits of detection (LODs) of 55.83, 71.51, 81.81, and 74.98 ng/mL, respectively. The recovery rates were 84.09–104.68%. This study provided new insight into the design of novel single-atom nanozymes for cascaded catalytic detection and other rapid detection applications with high efficiency and low cost.
研究领域:
分析化学
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环境科学
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纳米技术
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材料科学
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食品安全
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2021
·Vol. 413
·期 12
·3299-3313
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IF 4.157
Wang Milan,Song Zhihua,Jiang Youwei,Zhang Xiaolin,Wang Lin,Zhao Hongyu,Cui Yutong,Gu Furong,Wang Yunhua,Zheng Guoxia
Here, we present the rational design of a pinwheel-shaped three-dimensional microfluidic paper-based analytical device (3D-μPAD) for specific, sensitive and multiplexed detection of heavy metals in coastal waters. A more homogeneous permeation of fluids along the chip than common design, even under unskilled performance, has been achieved by the elaborate chip design of the hydrostatic balancing inlet port and uniformly stressed reversible sealing. With the combination of ion imprinted polymer grafted CdTe quantum-dots and fluid accumulation pad, 4 metals (Cu 2+ , Cd 2+ , Pb 2+ , and Hg 2+ ) in 1 analysis and 25-fold enrichment for each metal can be simultaneously performed within 20 min, with detection limits of 0.007–0.015 μg/L. It has the ability to selectively recognize these 4 metals in mixtures and immunizing to interferences from components found in coastal waters, which provided results that were in agreement with values gained from atomic absorption. The inexpensive and portable nature as well as the highly sensitive and flexible performance of the new developed 3D-μPAD could make it attractive as an on-site testing approach for marine environmental monitoring. Graphical abstract
研究领域:
分析化学
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环境科学
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微流控技术
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材料科学
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DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 12
·2901-2907
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IF 3.864
Wenyue Xie,Lingli Lei,Meiling Tian,Zeying Zhang,Yingshuai Liu
A high-resolution colorimetric immunoassay platform has been developed based on enzyme-catalyzed multicolor generation and smartphone-assisted signal readout. The multi-color generation is accomplished in this system through the urease-catalyzed urea hydrolysis-induced color change of the pH indicator phenol red, from yellow to orange to red over pH 6.6 to 8.0. The color change is easily tailored by controlling the urease activity via the inhibitor silver ion (Ag+), the amount of which is in turn adjusted by alkaline phosphatase (ALP)-catalyzed ascorbic acid (AA) production. An ALP-linked colorimetric immunoassay is readily realized based on the above urease catalyzed multicolor generation system. Under optimal conditions, a limit of detection (LOD) of 1.73 ng mL−1 and a dynamic range from 0 to 18 ng mL−1 are achieved with rabbit IgG as a model analyte. A colored picture for each test is directly taken using a smartphone and then quantitatively analyzed with the free software ImageJ, eliminating the use of expensive and desktop equipment. The dose-dependent multicolor display is easier to distinguish with the naked eye for qualitative or semiquantitative detection over the traditional one-color system. The developed immunoassay scheme provides a promising platform for on-site testing or applications in resource-poor areas.
研究领域:
分析化学
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生物传感
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即时检测
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移动健康
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2020
·
IF 10.652
Guangming Lu,Shu Tian,Jingyu Li,Yongjian Xu,Shuan Liu,Jibin Pu
Antifouling coatings are crucial to protect marine facilities and aquacultures from the damage of microorganisms for long-term serves. However, it is still a challenge to fabricate coating with excellent antifouling performance and superior mechanical stability. Here, we report a bio-based amphiphilic hydrogel coating with excellent antifouling and mechanical properties. The coating was achieved by the in-situ formation of hydrophobic and hydrophilic interpenetrating polymer network (IPN). The hydrophobic part from a synthesized silicon-containing epoxy resin contributes to superior mechanical properties including high tensile strength and excellent adhesion of 5B. While the hydrophilic hydrogel part from the cross-linking of a hydrophilic polymer with AgNPs gives excellent antifouling properties, resist to proteins, bacteria, algae, and other marine organisms. The antifouling performance of the coating was evaluated by the attachment of proteins (BSA-FITC), bacteria ( Escherichia coli and Bacillus subtilis ), and Algae ( Navicula torguatum and Phaeodactylum tricornutum ). The results show that the bio-based amphiphilic hydrogel coating (e.g. SA-1-5) endows surface with excellent resistance to the fouling of proteins, bacteria and microorganisms. The overall antifouling and mechanical properties of the amphiphilic coating was also evaluated by field test in East China Sea from June 3rd 2020 to July 17th 2020 that the bio-based amphiphilic hydrogel coating was intact and almost no marine organisms attached. This work provides a new strategy for the fabrication of bio-based and high-performance antifouling coatings.
研究领域:
材料科学
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海洋生物学
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化学工程
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生物技术
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DOI
高影响因子
VIBRATIONAL SPECTROSCOPY
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2021
·
IF 2.507
Lei Zhang,Yingshan Chen,Qin Zhu,Wenjin Ji,Suqing Zhao
Bisphenol A (BPA) is a typical endocrine-disrupting chemical (EDC), which has been banned for food packing and baby bottle material due to its potential endocrine harm to human body. In this paper, an easy and rapid immunochromatographic assay (ICA) combined with the technology of surface-enhanced Raman spectroscopy (SERS) was fabricated to improve the sensitivity and rapidity for quantitative determination of BPA. After 10 min of a quick chromatographic process, the specific Raman signal on T line of the test strip was measured. Compared with the traditional ICA, the SERS-ICA realized quantitative test and showed the limit of detection (LOD) was 0.1 ng/mL, getting approximately 300-fold lower. The method displayed high specificity and precision with RSD of 4.1%, and the test strip indicated favorable stability for two weeks without loss of activity. For river, tap and commercial drinking water sample, the SERS-ICA results were confirmed by HPLC, and the recoveries of spiked BPA were from 90.80-117.80%. It was shown that the proposed method takes advantages both of SERS and ICA, exhibiting high sensitivity, precision, stability, quantification and rapidity for field test, expecting a widely useful method for various small molecules in food safety, medical diagnosis and environmental samples.
研究领域:
分析化学
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环境科学
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食品安全
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医学诊断
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）