Zixian He,Zhiwei Liu,Haihuan Xie,Pengjie Luo,Xiangmei Li
Herein, an ultrasensitive lateral flow immunoassay (LFIA), based on metal-organic framework-decorated polydopamine (PCN-224@PDA) was first established to detect multiple sulfonylureas (SUs) in functional foods. The PCN-224@PDA was synthesized using the one-pot hydrothermal method and covalently coupled with SUs antibodies, and the coupling rate was up to 91.8%. The detection limits of the developed PCN-224@PDA-LFIA for multiple SUs in functional teas and capsules were 0.22–3.72 μg/kg and 0.40–3.71 μg/kg, and quantification limits were 0.75–8.19 μg/kg and 1.03–9.08 μg/kg, respectively. The analytical sensitivity was 128-fold higher than that of similar methods reported so far. The recovery rates ranged from 83.8 to 119.0%, with coefficients of variation of 7.6–14.4%. The parallel analysis of 20 real samples by LC-MS/MS confirmed the reliability of the proposed method. Therefore, our work offers novel, ultrasensitive, and rapid technical support for on-site monitoring of SUs in functional foods.Keywords:sulfonylureas;metal-organic frameworks;polydopamine;lateral flow immunoassay;functional foods
Haihuan Xie,Yingying Li,Jin Wang,Yi Lei,Anastasios Koidis,Xiangmei Li,Xing Shen,Zhenlin Xu,Hongtao Lei
Sulfonylureas (SUs) are a series of anti-diabetic drugs widely used for type 2 diabetes mellitus for clinic treat. However, it is often illegally adulterated in multi-herbal tea to improve the claimed anti-diabetic activity in recent years. In this study, a novel hapten was rationally designed, and a broad-specific monoclonal antibody (anti-SUs mAb) recognizing nine SUs was developed. This mAb was used to develop a colloidal gold lateral flow immunochromatographic assay (CG-LFIA). The anti-SUs mAb demonstrated half inhibitory concentration (IC 50 ) ranged from 0.15 ng/mL to 3.25 ng/ mL for nine SUs by ELISA. The cut-off value of developed CG-LFIA for nine SUs was from 3 to 100 ng/ mL for the spiked samples. LC-MS/MS confirmed the reliability of the new CG-LFIA. The results indicated that the proposed CG-LFIA could be an ideal method in on-site screening surveillance assay for SUs illegally adulterated in multi-herbal tea products.
The pivotal role of plasmid-mediated conjugative transfer in the spread of antibiotic resistance genes (ARGs) is well established, and environmental chemicals exert considerable selective pressure on this process. Oral antidiabetic drugs (OADs) are frequently detected in aquatic environments at concentrations reaching the mg/L range. However, their influence on the conjugative transfer of plasmid remains largely unexplored. Here, we investigated the effects of eight widely used OADs on the transfer of the RP4 plasmid between Escherichia coli strains isolated from representative wastewater sources. The results showed that all tested OADs significantly enhance plasmid transfer ( P < 0.05), albeit with up to a four - fold variation in change. Notably, this phenomenon is independent of OAD degradation profiles, but strongly associated with bacterial biochemical responses ( P < 0.05) such as reactive oxygen species (ROS) production, cell membrane permeability, and ATP synthesis. Mechanistic analyses further identify OAD electrophilicity as a key chemical determinant, modulating both physiological processes (membrane permeability and ATP production) and molecular components—genes ( ybbA, mlaA , and ompF ) and their corresponding proteins (YbbA, MlaA, and OmpF)—involved in conjugative transfer. Furthermore, results demonstrate that OADs consistently increase ARG abundance and diversity in activated sludge systems, an effect strongly correlated with their electrophilicity ( P < 0.05). Collectively, our findings reveal, for the first time, that OAD electrophilicity is a critical driver of ARG dissemination in wastewater treatment systems, thereby providing molecular-level insights and a theoretical foundation for controlling ARG spread.
危险品化学品经营许可证（带存储）