CROP PROTECTION
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2021
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IF 2.571
Feng Zheng,Tiantian Li,Hanhong Xu,Pengtong Hu,Ruifei Wang,Zhixiang Zhang,Jinliang Jia
Using plant essential oil instead of chemical pesticides to control melon fly ( Bactrocera cucurbitae (Coquillett)) is still a great challenge in agricultural practices, which must effectively slow down the volatility rate of plant essential oil. In this study, citral as a natural plant essential oil was blended with environmentally friendly castor oil based waterborne polyurethane (PU). The interaction, chemical resistance, adhesion property and sustained release behavior of the composites with different citral content were clarified, and the efficacies against the melon fly under field conditions were explored. The results indicated that the composites prepared exhibited excellent adhesion behavior on bitter gourds ( Momordica charantia L.) even under rainfall washing, meanwhile the chemical resistance of composites could be tailored via controlling citral content. Low citral content composite films could be washed off by alcohol without worrying about the safety of that on crops. The composite prepared exhibited long-lasting repellent behavior against melon fly. The composite can be prepared via a simple technology and low cost, which has great value for plant essential oil applied in agriculture and can provide a new approach for melon fly control.
研究领域:
农业科学
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昆虫学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Insects
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2025
·Vol. 16
·期 7
·716
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IF 2.9
Lei Li,Dongyin Han,Jing Zhao,Haiyan Qiu,Fangping Zhang,Zhengpei Ye,Yueguan Fu
Simple SummaryThe melon fly (Zeugodacus cucurbitae) is a pest that prefers to bore into and feed on cucurbitaceous crops and that pupates in the soil. The parasitoidSpalangia endiuscan burrow into the soil to attack melon fly pupae, but it only kills about 33% of them. Since insecticides are essential for controlling melon fly populations, we assessed how five commonly used insecticides affect the toxicity, survival, reproduction, choice-making behaviour, and parasitism ability ofS. endius. Our goal was to determine how to combine insecticides withS. endiusto more effectively control melon fly pupae. Through a series of laboratory and semi-field trials, we confirmed that abamectin is suitable for combined use withS. endius. However, we must be careful about the concentration and timing of abamectin application. The best approach is to spray abamectin on the soil surface before the melon flies pupate and then release the parasitoids.Spalangia endiusWalker, a pupal parasitoid of the alien invasive pestZeugodacus cucurbitae(Coquillett), causes 33% host mortality. This study assessed whether combiningS. endiuswith insecticides (abamectin, thiamethoxam, nitenpyram, emamectin benzoate, or beta-cypermethrin)—all effective againstZ. cucurbitae—could enhance control efficacy. Among these, abamectin was the least toxic to adultS. endius. Surface contact treatments with 12 and 15 mg a.i./kg of abamectin did not significantly increaseS. endiusmortality. However, mixing 12 mg a.i./kg of abamectin into a honey solution to encourage ingestion decreased the survival, parasitism, and fecundity ofS. endius. In olfactometer assays,S. endiusadults avoided abamectin-treated host pupae, though prior exposure to abamectin mitigated this avoidance. The timing of abamectin soil application relative to host pupation andS. endiusrelease affects host mortality. The most effective timing is spraying abamectin before host pupation (to exposeZ. cucurbitaelarvae) and then releasingS. endius. Field trials confirmed that combining abamectin (12 mg a.i./kg) withS. endiusincreased host mortality more than either treatment alone. In conclusion, abamectin (12 mg a.i./kg) is a suitable insecticide for combination withS. endiusto controlZ. cucurbitae.The application sequence should be spraying abamectin before hosts pupate and, only after that, releasing the parasitoid.
研究领域:
昆虫学
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生物防治
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害虫管理
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杀虫剂
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寄生性天敌
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2024
·
IF 3.8
Yun Wang,Hui-Qian Xu,Hong-Liang Han,Dong Chen,Hongbo Jiang,Guy Smagghe,Jin-Jun Wang,Dong Wei
BACKGROUND The melon fly, Zeugodacus cucurbitae (Coquillett), is an invasive Tephritidae pest with robust fertility. The male accessory glands (MAGs) form a vital organ that ensures insect reproductive efficiency. Most of the secreted proteins by MAGs exhibit a male bias expression. Takeout, one of these proteins, is abundantly present in the MAGs of many insects. RESULTS In this study, we identified 32 takeout genes in Z. cucurbitae . The phylogenetic analysis and multiple sequence alignment results showed that Zctakeout1 is the most related homolog to the MAGs-specific takeout in Tephritidae. The real-time quantitative PCR results showed that Zctakeout1 was exclusively expressed in the male adult stage, and its expression level gradually increased with the increase in age and then remained stable at the sexually matured stage. The distribution among tissues demonstrated the specific expression of Zctakeout1 in the MAGs, and fluorescence immunohistochemical results confirmed the presence of Zctakeout1 in close proximity to binuclear cells of the mesoderm epidermal MAGs. In continuation, CRISPR/Cas9-mediated genome editing was employed, resulting in successfully generating a homozygous strain with an +8 bp insertion. The mating experiments with the Zctakeout1 −/− males resulted in significant reductions in both the mating rate and egg production of females. CONCLUSION These findings prove that the MAGs-specific Zctakeout1 is essential in regulating fecundity in female Z. cucurbitae fruit flies. Our data suggests its utilization in future essential insect-specific gene-directed sterility insect technique (SIT) by the genetic manipulation to keep these important Tephritidae populations under control. © 2024 Society of Chemical Industry.
研究领域:
昆虫学
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遗传学
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分子生物学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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