MOLECULES
·
2019
·Vol. 24
·期 8
·1474
·
IF 3.06
Kai Zhou,Lorenzo Siroli,Francesca Patrignani,Yuanming Sun,Rosalba Lanciotti,Zhenlin Xu
The aim of this work was to clarify the formation of ethyl carbamate (EC) and its influence factors throughout the production process of Cantonese soy sauce. The results showed that EC was not detected in thekoji-making and earlymoromifermentation stages, but started to be generated when pH of themoromidecreased to about 4.9—at the same time, the levels of ethanol, urea and citrulline increased significantly. Most EC was formed during raw soy sauce hot extraction (40.6%) and sterilization (42.9%) stages. The EC content exhibited the highest correlation with ethanol throughout the whole production process (R = 0.97). The simulation soy sauce produced in laboratory led the same conclusion—moreover, the contents of EC, ethanol and citrulline were higher in soy sauce fermented at 30 °C than in soy sauce fermented at 15 °C. Extraction of raw soy sauce by squeezing contributed little to EC formation. Further research showed that citrulline and ethanol led to significant increases in EC levels in raw soy sauce upon heating. These results indicate that ethanol and citrulline are two critical precursors of EC and that EC is mainly formed during the heat treatment stage of soy sauce.Keywords:ethyl carbamate;soy sauce;precursor;ethanol;accumulation stage
研究领域:
食品科学
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生物化学
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PubMed
高影响因子
AMINO ACIDS
·
2025
·Vol. 57
·期 1
·1-15
·
IF 2.4
Wang Jiteng,Zhang Jingyi,Li Xinyu,Xu Hanying Y.,Yang Yeshun,Zhang Jiankun,Feng Wenping,Chen Qiang,Dong Fen,Han Tao
The main objective of this experiment was to study the metabolism of arginine in juvenile largemouth bass ( Micropterus salmoides ). A total of 300 healthy fish (average weight of 25 ± 0.5 g) were randomly assigned to ten groups. Experimental fish were orally administered or intraperitoneally injected with 0.9% sodium chloride, arginine, arginine-aspartate, citrulline, and glutamate solutions, respectively. They were euthanized at 10, 30, 60, 120, and 240 min after oral administration or intraperitoneal injection, and various tissue samples were subsequently collected for analysis. The results revealed that serum ornithine and citrulline concentrations of largemouth bass were significantly increased by oral administration of arginine or arginine-aspartate ( P < 0.05). Intraperitoneal injection of arginine or arginine-aspartate solution significantly elevated the concentrations of ornithine and citrulline in the serum, liver, kidney, and muscles ( P < 0.05). The concentrations of citrulline, ornithine, and arginine in serum and muscle increased significantly at 4 h after intraperitoneal injection of glutamate ( P < 0.05). Intraperitoneal injection of citrulline significantly increased the concentrations of ornithine and arginine in the serum and muscles ( P < 0.05). The research findings demonstrate that both free and small peptide forms of arginine were rapidly degraded to ornithine due to the high arginase activity in various tissues of largemouth bass. Additionally, the pathway of synthesizing citrulline from glutamate and then arginine from citrulline may exist in largemouth bass, but the exact location of this synthesis process may differ from that found in mammals.
研究领域:
水产养殖学
·
生物化学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
·
2022
·
IF 2.731
Huimin Li,Yapan Shi,Yudong Gao
A new voltammetric sensor, simultaneous electropolymerization of L-Citrulline (L-Cit) and β-cyclodextrin (β-CD) film modified glassy carbon electrode (GCE) was built for the first time. It was used to construct this sensor for quantification of these important pesticides and sensitive determination of metribuzin. The proposed metribuzin sensor displays excellent performance with wide linear range (3.0 × 10−8 ~ 1.0 × 10−6 mol L−1), ultralow detection limit of 10 nM (S/N = 3), and favourable specificity. Also, the sensor was applied for determination of metribuzin in the real samples, the satisfying results are achieved. The results imply that the proposed sensor can be potentially used for the environmental pollution science and environmental analytical chemistry.
研究领域:
环境科学
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分析化学
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高影响因子
Animals
·
2023
·Vol. 13
·期 2
·217
·
IF 3
Guodong Zhao,Xi Zhao,Jiachen Bai,Airixiati Dilixiati,Yukun Song,Aerman Haire,Shangshang Zhao,Aikebaier Aihemaiti,Xiangwei Fu,Abulizi Wusiman
Simple SummaryOur previous study found that the addition ofL-citrulline (L-Cit) to feed improved ram semen density as well as vitality and sperm mitochondrial membrane potential. In this experiment, after feeding 12 g/d ofL-Cit to rams for 70 consecutive days, the contents of amino acids, sugars, and pyruvate in seminal plasma, in addition to the changes in metabolites in plasma, were analyzed by metabolomics, and the differentially expressed genes in the hypothalamus and testes were further studied through a transcriptome analysis. The results showed thatL-Cit could increase the contents of pyruvate and most amino acids in seminal plasma, and that the differentially expressed genes in the hypothalamus and testes were significantly enriched in protein-metabolism-related pathways. This shows thatL-Cit can improve the contents of amino acids in rams’ semen by promoting the protein metabolism of the hypothalamus–testes axis, thus improving semen quality.AbstractThis study examined the effects ofL-Cit supplementation on ram semen quality through metabolomics and transcriptomics. A total of 16 rams were randomly categorized into two groups. The control group was fed a basic diet, whereas the experimental group received feed supplemented with 12 g/d ofL-Cit. Semen and blood were collected from the rams on days 0 and 72 to measure sugar, pyruvate, amino acid, and nontargeted metabolite contents. Additionally, hypothalamic and testicular tissues were collected for a transcriptomic analysis. We found 27 differential metabolites between the control and experimental groups, of which 21 were downregulated (p< 0.05) and 6 were upregulated (p< 0.05). Compared with the control group, xylose and pyruvate contents in seminal plasma increased by 43.86% and 162.71%, respectively (p< 0.01). Additionally, the levels of 11 amino acids showed a significant increase in seminal plasma (p< 0.01). Furthermore, 961 and 715 differentially expressed genes were detected in the hypothalamic and testicular tissues, respectively. The pathways of significant enrichment in the hypothalamus and testes were protein digestion, absorption, glycolysis/gluconeogenesis, and amino as well as nucleotide sugar metabolisms. In the present study,L-Cit improved protein synthesis and blood metabolism, consequently increasing the contents of most amino acids in ram seminal plasma. Specifically, the hypothalamus controlled the expression of glycolysis/gluconeogenesis-related genes in the testes through its metabolites released into the serum, thereby providing energy for sperm production, which led to a decrease in the sugar content of seminal plasma.Keywords:metabolome;transcriptome;L-Cit;semen quality regulation;rams
研究领域:
动物生理学
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生殖生物学
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PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 8
·086508
·
IF 3.721
Suxia Wang,Min Zhou,Yongxi Zhu,Bo Peng,Jia Yang,Yongjun Ma
In this study, a novel poly(L-citrulline)/lanthanide-containing cyano-bridged heterometallic coordination polymer modified glassy carbon electrode, poly(L-Cit)/(Ho(III)-Fe(III)-WO42− CyHMCP/GCE, was constructed as a sensing platform for the electrochemical detection of 3-nitropropionic acid (3-NPA) using the two-step electrosynthesis method. The surface morphology of materials coated on the chemically modified electrode was characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Based on the excellent synergistic electrocatalytic performance for electrochemical reduction of 3-NPA, the voltammetric detection of 3-NPA in real sugarcane juice samples was achieved with a facile sample pretreatment process and less matrix interference. Furthermore, by means of water-phase infrared spectra technique, the electrocatalytic reduction pathway of 3-NPA was preliminarily studied. Under the optimized detection conditions, a good linear relationship was obtained between the reduction peak current of 3-NPA and testing concentration in the range of 40.0–4.00 × 103 μM, with a regression equation of Ip (μA) = −3.126 + 0.08804C3-NPA (μM), R2 = 0.9997. The lower detection limit (LOD) of 3-NPA was estimated to be 16.0 μM (S/N = 3). Therefore, the method had been employed to analyze raw sugarcane samples, with the average recovery of 96.8%–104%, and the actual concentration level of free 3-NPA in the raw sugarcane juices was revealed.
研究领域:
分析化学
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食品科学
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电化学
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DOI
高影响因子
Frontiers in Microbiology
·
2021
·
IF 5.64
Zhou Kai,Zhang Xiao,Li Bingyong,Shen Chaoqun,Sun Yuan-Ming,Yang Jianyuan,Xu Zhen-Lin
Citrulline is one of the major precursors of ethyl carbamate in soy sauce, and the accumulation of citrulline is attributed to the metabolism of arginine by bacteria with the arginine deiminase (ADI) pathway. However, key strains and factors affecting citrulline accumulation are not yet clear. In this study, two key strains of Pediococcus acidilactici and Weissella confusa were isolated from soy sauce moromi, and the regularity of citrulline formation was studied. Results showed that the conversion rates from arginine to citrulline (A/C rate) and the citrulline accumulation ability of W. confusa and P. acidilactici significantly increased in the presence of different concentrations of NaCl, indicating that salt stress was the main factor for citrulline accumulation. The inconsistent expression of arc genes by salt stress was the reason for citrulline accumulation for P. acidilactici, but for W. confusa, it may be due to the influence of arginine/citrulline on the transportation system: the intracellular citrulline could neither transport to extracellular space nor convert into ornithine. Environmental factors greatly influenced citrulline accumulation of the two key bacteria; A/C rate and citrulline formation in both strains decreased at low temperature (15°C) under high salt stress, but opposite effects were observed for the two key strains under anaerobic light condition. Moreover, quercetin and gallic acid significantly decreased the A/C rate and citrulline accumulation ability of the two key strains. The optimal quercetin and gallic acid as suggested by simulation experiment were 100 and 10 mg/l, respectively, and the lowest A/C rate of 28.4% and citrulline level of 1326.7 mg/l were achieved in the simulation system. This study explored the main factors for citrulline formation by the two key strains and proposed a targeted way to control citrulline in soy sauce.
研究领域:
食品科学
·
微生物学
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PubMed
高影响因子
TALANTA
·
2015
·
IF 3.545
Wenjing Wang,Lu Wang,Lina Zou,Gaiping Li,Baoxian Ye
A novel voltammetric sensor was constructed by simple dripping single-walled carbon nanotubes (SWCNTs) on to the glass carbon electrode (GCE) firstly and electro-polymerizing l -Citrulline film subsequently. The resulting poly( l -Citrulline)/SWCNTs/GCE showed a significant voltammetric response to picroside II due to the synergistic effect of SWCNTs and poly( l -Citrulline) film. The first electroanalytical method of picroside II was proposed with detection linear range from 8.0×10 −8 to 5.0×10 −6 mol L −1 and a detection limit of 3×10 −8 mol L −1 .The high sensitivity, selectivity and long-term stability made the sensor suitable for the determination of picroside II. Moreover, based on the systematically investigation and some kinetics parameters calculated in the experimentation, the reaction mechanism of picroside II at the poly( l -Citrulline)/SWCNTs modified GCE was obtained reliably. Lastly, the proposed sensor was used for the determination of picroside II in real sample with satisfactory results. This work promoted the potential applications of amino acid materials and SWCNTs in electro-chemical sensors.
研究领域:
分析化学
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生物医学工程
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PubMed
高影响因子
Nature Aging
·
2025
·
IF 19.4
Liu Liu,Wang Qianyue,Chen Meng,Zhou Haifeng,Li Xu,Yuan Ziqi,Hu Yong,Wang Chaoqun,Zhang Xiaohui,Hu Sheng,Wu Xiaoting,Li Meixin,Li Juanjuan,Chen Aijun,Yan Wei
Lung aging is a multifactorial series of molecular alterations that leads to gradual deterioration of lung function and increased vulnerability to cancer. Tumor communicates with host organs partially through extracellular vesicles; however, the mechanistic drivers and consequences of lung aging in the context of cancer remain unclear. Here we identify cancer cell-secreted dimethylarginine dimethylaminohydrolase-1 (DDAH1) protein induces citrulline accumulation and promotes lung fibrosis and aging. Mechanistically, our single-cell sequencing and genetic knockout mice evidence that citrulline availability elevation inhibits peptidyl arginine deiminase 4-mediated transforming growth factor-β1 (TGF-β1) citrullination, thereby inducing the TGF-β1/Smad3 signaling pathway in lung fibroblasts. Notably, vacuolar protein sorting assists the packaging of DDAH1 into the late endosomes. The administration of DDAH1 inhibitor reduces fibrosis and alleviates lung aging. Conclusively, our findings reveal tumor-derived DDAH1 protein contributes to citrulline accumulation to promote lung aging, shedding light on the treatment and diagnosis of tumors by inhibiting senescent lung fibroblasts.
研究领域:
肿瘤学
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呼吸系统生物学
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分子生物学
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细胞衰老
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信号转导
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转化医学
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肿瘤微环境
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PubMed
高影响因子
Metabolites
·
2022
·Vol. 12
·期 7
·610
·
IF 5.581
Lin-Lin Cao,Yi Han,Lin Pei,Zhi-Hong Yue,Bo-Yu Liu,Jing-Wen Cui,Mei Jia,Hui Wang
Type 2 diabetes mellitus (T2DM) has been identified as an independent risk factor for hepatocellular cancer (HCC). However, there are no ideal biomarkers for the surveillance and early detection of HCC in the T2DM population at present. In this study, we aimed to explore novel metabolite biomarkers for T2DM-positive [T2DM(+)] HCC by metabolomic analysis. At first, many serum metabolites were found dysregulated in T2DM(+) HCC patients in untargeted metabolomic analyses. Targeted metabolite analyses confirmed that serum benzoic acid and citrulline were increased, and creatine was decreased in T2DM(+) HCC compared to the T2DM group. A metabolite classifier including benzoic acid, creatine, and citrulline was identified as a novel biomarker for the diagnosis of T2DM(+) HCC, with an area under the ROC curve (AUC) of 0.93 for discriminating T2DM(+) HCC patients from T2DM patients. In addition, the metabolite classifier detected small-size (AUC = 0.94), early-stage (AUC = 0.94), and AFP-negative (AUC = 0.96) tumors with high sensitivity and specificity. The combination of this metabolite classifier and AFP might be useful in the surveillance and early detection of HCC in the T2DM population. In conclusion, this study establishes a novel diagnostic tool for T2DM(+) HCC.Keywords:hepatocellular cancer;type 2 diabetes mellitus;metabolomics;metabolite classifier;benzoic acid;creatine;citrulline
研究领域:
肿瘤学
·
糖尿病学
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代谢组学
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生物标志物研究
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 193
·期 1
·28
·
IF 5.3
Li Yixian,Yang Xiaohui,Cheng Shanshan,Mu Rongrong,Li Huiqiang,Yu Yang
Autoantibodies play a critical role in predicting diseases and monitoring therapeutic outcomes. Limitations of current autoantibody detection techniques include prolonged detection times and operational complexity. This study aims to develop a bidirectional microfluidic platform for the detection of autoantibodies. A “double inlet” and “waste outlet” structure was developed on the bidirectional lateral-flow microfluidic chip (BiLFMC). Using anti-citrullinated peptide antibodies (ACPA) as a model, this study validated the feasibility and performance advantages of the BiLFMC in the diagnosis of autoimmune disease. The BiLFMC was optimized by selecting the optimal distances between the sample inlet and waste outlet, as well as the ideal diameter for the waste outlet. The most suitable absorbent material was chosen for the waste absorbent pad. The detection and anti-interference performance of the unidirectional mode, unidirectional sequential mode, and bidirectional mode were compared, demonstrating that the bidirectional microfluidic chip significantly enhanced anti-interference capabilities. For ACPA detection, a serum sample required only a 1:100 dilution and 10 μL volume, with results available in 8 min. The coefficient of variation (CV) for repeatability ranged from 7.04% to 13.55%, while the CV for intermediate precision ranged from 11.06% to 14.49%. The limit of quantitation was 3.951 RU/mL, and the upper limit of the linear range was 500 RU/mL. When compared to ELISA results, the consistency coefficient was 0.8302. Furthermore, the BiLFMC was able to accurately distinguish samples exceeding the ELISA upper limit (> 196 RU/mL). A bidirectional microfluidic chip has been developed, incorporating indirect detection methods to enhance the anti-interference capability of the microfluidic platform. Graphical abstract
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高影响因子
Journal of Genetics and Genomics
·
2024
·
IF 6.6
Xiaoya Zhang,Abhisek Bhattacharya,Chunxiang Pu,Yan Dai,Jia Liu,Lang Rao,Chaoguang Tian
Histone citrullination, an important post-translational modification mediated by peptidyl arginine deiminases, is essential for many physiological processes and epigenetic regulation. However, the causal relationship between histone citrullination and specific gene regulation remains unresolved. In this study, we develop a programmable epigenetic editor by fusing the peptidyl arginine deiminase PPAD from Porphyromonas gingivalis with dCas9. With the assistance of gRNA, PPAD-dCas9 can recruit peptidyl arginine deiminases to specific genomic loci, enabling direct manipulation of the epigenetic landscape and regulation of gene expression. Our citrullination editor allows for site-specific manipulation of histone H3R2,8,17 and 26 at target human gene loci, resulting in the activation or suppression of different genes in a locus-specific manner. Moreover, the epigenetic effects of the citrullination editor are specific and sustained. This epigenetic editor offers an accurate and efficient tool for exploring gene regulation of histone citrullination.
研究领域:
表观遗传学
·
分子生物学
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基因组学
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生物技术
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PubMed
高影响因子
Nutrients
·
2022
·Vol. 14
·期 21
·4549
·
IF 6.706
Tongtong Ba,Dai Zhao,Yiqin Chen,Cuiping Zeng,Cheng Zhang,Sai Niu,Hanchuan Dai
L-citrulline (L-cit) is a key intermediate in the urea cycle and is known to possess antioxidant and anti-inflammation characteristics. However, the role of L-cit in ameliorating oxidative damage and immune dysfunction against iron overload in the thymus remains unclear. This study explored the underlying mechanism of the antioxidant and anti-inflammation qualities of L-cit on iron overload induced in the thymus. We reported that L-cit administration could robustly alleviate thymus histological damage and reduce iron deposition, as evidenced by the elevation of the CD8+T lymphocyte number and antioxidative capacity. Moreover, the NF-κB pathway, NCOA4-mediated ferritinophagy, and ferroptosis were attenuated. We further demonstrated that L-cit supplementation significantly elevated the mTEC1 cells’ viability and reversed LDH activity, iron levels, and lipid peroxidation caused by FAC. Importantly, NCOA4 knockdown could reduce the intracellular cytoplasmic ROS, which probably relied on the Nfr2 activation. The results subsequently indicated that NCOA4-mediated ferritinophagy was required for ferroptosis by showing that NCOA4 knockdown reduced ferroptosis and lipid ROS, accompanied with mitochondrial membrane potential elevation. Intriguingly, L-cit treatment significantly inhibited the NF-κB pathway, which might depend on restraining ferritinophagy-mediated ferroptosis. Overall, this study indicated that L-cit might target ferritinophagy-mediated ferroptosis to exert antioxidant and anti-inflammation capacities, which could be a therapeutic strategy against iron overload-induced thymus oxidative damage and immune dysfunction.Keywords:L-citrulline;thymus;iron overload;ferritinophagy;ferroptosis
研究领域:
免疫学
·
药理学
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细胞生物学
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生物化学
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PubMed
高影响因子
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