INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Xunming Zhang,Jiayuan Fang,Qinchuan Lyu,Tong Su,Xingyu Xiao,Libo Zhang,Shuo Zheng,Yi Li,Linlin Hao,Huayi Lu
Effective co-expression of mussel adhesion protein fp-151 and tyrosinase of Verrucomicrobium spinosum (tyrVS) in E. coli . • The introduction of copper ion in the induced expression process effectively improved the adhesion performance of fp-151 • The new strategy provides a valuable reference for the development of biological adhesive based on recombinant mussel adhesion protein. Mussel adhesion proteins are widely used in biomedical fields due to their wet adhesion properties. However, tyrosine residues have been modified with limited efficiency in the development of recombinant mussel adhesins. In this study, the recombinant fp-151 and tyrosinase of Verrucomicrobium spinosum (tyrVS) were successfully co-expressed in E. coli for the first time. Moreover, we systematically explored whether the increased activity of tyrVS caused by the introduction of copper ions during the induction process could effectively improve the adhesion property of fp-151, and the addition of copper ions was applied to the expression of fp-151. The results indicate that the introduction of copper ions effectively increased the activity of tyrVS to 39.33 U/mg protein without affecting the expression of fp-151. Through NBT/glycine staining, surface coating investigations, and batch adhesion strength analyses, this strategy significantly improved the DOPA level in fp-151, resulting in enhanced adhesion performance, with batch adhesion strength increasing by 1.5 times. Furthermore, the sample demonstrated the ability to adhere to HaCaT cells without causing additional cytotoxicity. Therefore, this strategy may provide a crucial foundation for the development of recombinant mussel adhesive proteins.
✓ 已验证
产品关联
DOI
高影响因子
Vaccines
·
2026
·Vol. 14
·期 3
·276
·
IF 3.5
Bin Wang,Kehui Wang,Zhiyao Xu,Weihua Liu,Xianhuang Li,Linhao Li,Renhui Zhou,Xingyue Du,Jin Jin,Yaqing Xu,Rihui Qin,Xiong Liu,Dayang Zou,Wei Liu
Background:The global spread of monkeypox virus (MPXV) highlights an urgent need for thermostable and easily administrable vaccines. Current orthopoxvirus vaccines are limited by cold-chain dependence and inconvenient injection-based delivery.Objectives:This study aimed to develop a dissolvable microneedle (DMN) vaccine against monkeypox based on a replication-deficient orthopoxvirus platform, through systematic formulation screening, stabilization mechanism exploration, and rigorous in vivo immunogenicity evaluation.Methods:A film-based approach was adopted for efficient, high-throughput formulation screening and thermostability assessment. NTV was mixed with excipients and dried into solid films. Stability was monitored via RT-qPCR after storage at 4 °C to 40 °C. The lead formulation was physically characterized, then used to fabricate MVA-BN-loaded DMN patches, which were further evaluated for in vivo immunogenicity via immunization inBALB/cmice.Results:The optimal formulation F2 (containing dextran, L-threonine, and BSA/HSA) showed a potency loss of only ~1 log10after 2 months at 25 °C, and <1 log10loss after 1 week at 37 °C. SEM revealed a porous virus-entrapment morphology, and FTIR indicated enhanced hydrogen bonding between the virus and the dextran matrix. The formulation was successfully manufactured into DMNs that dissolved within 5 min. In mice, these DMNs elicited robust MPXV-specific IgG and neutralizing antibody responses, with immunogenicity comparable to that induced by conventional intramuscular injection.Conclusions:This study successfully established a thermostable formulation and dissolvable microneedle delivery platform for replication-deficient orthopoxvirus vaccines against monkeypox. The optimized DMN vaccine induced robust MPXV-specific immune responses in mice with immunogenicity comparable to intramuscular injection, addressing the core limitations of current vaccines and providing a promising solution for monkeypox prevention.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL GEOLOGY
·
2019
·
IF 3.618
Ying Wang,Xiu Yuan,Xiaomin Li,Fangbai Li,Tongxu Liu
Cytochrome c ( c -Cyts), as a key protein involved in extracellular electron transfer , plays an important role in the microbial Fe(II)-oxidizing process. In this study, due to their similar structure with the outer membrane protein of microorganisms , the equine c -Cyts was employed as a model protein to examine the impact of different organic ligands on the redox kinetics between Fe(II) and c -Cyts using the rapid stopped-flow spectrometer. Our results suggest that the presence of organic ligands will significantly accelerate the reaction between c -Cyts and Fe(II), with reaction rates obtained in the following order: EDTA > citric acid (Cit) > NTA > malonic acid (Mal) > glycine amino acid (Gly) > acetate acid (Ace) > control. It was also found from the kinetic modeling results that the reduction rate of c -Cyts by Fe(II)-ligands was closely correlated to the complex constant between Fe(II) and each ligand, and the reduction rate constants of c -Cyts by Fe(II)L species (L represents “ligands”). Further analysis on the ligand structure indicates that the reaction rate constants are highly correlated with the complex constants of Fe(II)-ligand and the reduction rate constants of c -Cyts by Fe(II)L species ( k 3 ) for Group I (Gly, NTA, and EDTA) and Group II (Cit, Mal, and Ace). The results of midpoint potentials of all Fe(II)-ligands obtained through cyclic voltammetry (CV) experiments were ranked as Ace < Gly < NTA < control < Mal < EDTA < Cit, indicating that the redox potentials of Fe(II) complexes in the same group with similar structures were positively correlated with Fe(II)-ligand complex constants. The reorganization energy change of the electron-transfer reaction resulting from the different Fe(II)-ligand structures may contribute significantly more than does the Gibbs free energy (Δ G ) to the reaction kinetics of Fe(II)-ligand and c -Cyts.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Yao Hui,Rukuan Liu,Yiming Gong,Jingwen Lan,Youhui Chen,Lijun Wu,Airong Xu
Conductive hydrogel represents an important development direction in modern/future electronic industry. However, successfully achieving high performance hydrogels which are capable of performing a real-world task still remain extremely challenging because most conventional approaches of constructing hydrogels are difficult to make hydrogels achieve the integration of multiple performances like robust mechanical properties, excellent conductivity and stable electrochemical performance. Here, we develop a simple and universal two-layer hydrogen bond networks (TLHBNs) strategy by which seven types of PVA based hydrogels with simultaneously robust mechanical properties (stress, strain, toughness, elasticity, fatigue resistance, notch insensitivity, anti-damage), excellent conductivity (7.2− 12.2 S m −1 , 11.7− 19.8 S m −1 , 10.5− 21.6 S m −1 ), reversible/stable network structure and constantly sensitive signal output capability are achieved via sophisticatedly layer-by-layer introducing TLHBNs to PVA hydrogel. Benefiting from the extraordinary performances, one hydrogel is so strong that it can load as heavy as 80 kg of a boy volunteer. Meanwhile, the hydrogels, assembled into flexible sensors, satisfactorily fulfills practical tasks (human joint motions and bionic skin) upon handling real-world application. This strategy, enabling extraordinary performances while using a generic and facile designing concept, opens up a new insight into the design of high-performance PVA hydrogel and beyond.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Xusheng Cheng,Tao Xuan,Haoran Hu,Jianchi Wang,Jiantao Zai,Liwei Wang
Alkaline zinc-based flow batteries suffer from poor zinc reversibility due to dendrite growth and parasitic reactions, which significantly shorten their cycling lifespan. A key challenge lies in the rational design of ligands to eliminate concentration polarization caused by mismatched diffusion and interfacial reaction rates, thereby inducing and regulating uniform zinc deposition. In this study, we propose a thermodynamic descriptor-guided ligand screening strategy, using the metal–ligand stability constant (log K ) as a quantitative criterion to simultaneously optimize deposition morphology and interfacial ion kinetics. Guided by this principle, nitrilotriacetic acid (NTA, log K = 11.98) is identified as a robust chelating agent under strongly alkaline conditions (pH > 14). Its moderate coordination strength enables the disruption of the native Zn 2+ –H 2 O network, effectively suppressing hydrogen evolution while maintaining near-theoretical Zn 2+ desolvation kinetics. In situ microscopy and electrochemical analyses reveal that NTA directs preferential Zn(002) growth, yielding dendrite-free deposition at ultrahigh current densities (80 mA cm −2 ) and high areal capacities (40 mAh cm −2 ). Furthermore, NTA facilitates efficient Zn 2+ diffusion (5.77 × 10 −7 cm 2 /s), outperforming strong chelators such as ethylenediaminetetraacetic acid. As a result, Zn//Zn symmetric cells exhibit stable cycling over 400 h, while NTA-enabled Zn Fe flow batteries achieve 700 cycles with 99 % coulombic efficiency and minimal capacity decay. This work establishes log K as a practical screening descriptor for multi-objective electrolyte optimization and provides a scalable pathway for the development of high-performance alkaline zinc flow batteries.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2024
·
IF 9.3
Airong Xu,Tiantian Sun,Rukuan Liu,Liuzemu Li,Yiming Gong,Zhihong Xiao
Versatile PVA/SG (polyvinyl alcohol/sodium glycinate) hydrogel sensors have been successfully developed using SG and PVA as cost-efficient and biocompatible starting materials. The mechanical properties significantly increase with increasing F–T (freezing–thawing) times and SG content in the PVA/SG hydrogel. The strengthened mechanical properties mainly result from the abundant hydrogen bond interactions of PVA with SG and the enhanced hydrogen bond crosslinking density endowed by F–T cycles and increased SG content. Moreover, the PVA/SG hydrogel shows excellent electrical conductivity (4.85 S m−1), realizing the excellent combination of high mechanical properties and electrical conductivity. Interestingly, as well as wonderful cytocompatibility, the hydrogel displays excellent swelling resistance and water tolerance, and the mechanical properties remain almost unchanged after immersion in water for 6 months. Most importantly, the hydrogel has high strain sensitivity with a gauge factor of 1.76, stable signal repetition monitoring, very good elasticity, fatigue resistance and recovery, and thus can be assembled into flexible sensors to monitor bending changes in human Knuckles, wrists and knees, and also be used to simulate manual control of a mobile phone screen for writing text. This study provides valuable information for the design and construction of wearable hydrogel sensors with high performance for practical applications.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）