FOOD CHEMISTRY
·
2024
·
IF 8.5
Chuang Yang,Yufei Zhang,Yuanzhi Xu,Yibin Zhou,Shiyi Li,Mingming Zheng
An applicable and highly efficient methodology for the preparation of medium- and long-chain triglycerides (MLCTs) via the enzymatic transesterification of coconut oil with long-chain fatty acid triglycerides, named camellia oil, olive oil, linseed oil, algal oil, and rapeseed oil, respectively, has been proposed. The novel system achieved equilibrium in 5 min, and the MLCT yield ranged from 78.7 to 83.8 %. This is the fastest record reported to date. The catalytic efficiency of the novel biocatalyst, CSL@HMS-C 8 , was 140 g/g•h, 1.43 times higher compared to the control (free lipase), and it retained 62.5 % of its relative activity after 15 cycles. A UPLC-MS provided an accurate structural analysis of the MLCTs, and molecular docking analysis was performed to understand the binding interactions between the protein and molecules. The method of preparing MLCT enriched with functional fatty acids in this paper is efficient, broadly applicable, economical, and promising for industrial applications.
研究领域:
生物化学
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食品科学
·
工业生物技术
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Arbab Ali,Mengjie Wang,Haoxiang Guo,Chao Jiang,Ze Wang,Xin Dai,Yunfeng Zong,Yuan Liu
Exposure of nanoparticles (NPs) to biological fluids inevitably results in rapid surface coating by biomolecules, mainly proteins, forming what is commonly termed the protein corona (PC). The PC confers a transformed biological identity to NPs that governs their interaction with physiological systems and influences the pharmacological profile. However, the role of blood metabolites in regulating PC and subsequent nanobio interaction remains undefined. In this study, we examine the impact of elevated triglyceride (TG) levels, clinically termed hypertriglyceridemia, on PC modulation and its related physiological response and pharmacokinetics. We observe that the elevated TG levels differentially regulate PC formation by promoting the adsorption affinity of specific proteins to NPs. The TG-rich PC induces a robust immune response in macrophages by stimulating the release of pro-inflammatory cytokines compared to normal-PC. In vivo experiments reveal that relative to normal mice, NPs in hypertriglyceridemic (HTG) mice show more accumulation and sequestration in the liver and spleen. Furthermore, PC formed in HTG mouse serum shows higher uptake in macrophages and hepatocytes than PC formed in normal mouse serum. Finally, the proteomic analysis reveals distinct disparities in protein adsorption patterns: Lipoproteins are more dominant in HTG-PC, whereas immunoglobulins and complement proteins are more prevalent with normal-PC. Overall, this work offers important insights into the predictive role of disease-related metabolites on the modulating PC and nanobio interaction, aiding in the rational design of nanomedicine.
研究领域:
纳米医学
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生物材料学
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分子生物学
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免疫学
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药理学
·
代谢疾病
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转化医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CLINICAL BIOCHEMISTRY
·
2020
·
IF 2.573
Tianhua Zhang,Xiaoyu Tang,Ling Mao,Jin Chen,Jie Kuang,Xin Guo,Danyan Xu,Daoquan Peng,Bilian Yu
Background The mechanism for an abnormal pattern of triglyceride (TG) metabolism in response to a meal still needs further investigation. Extensive pieces of evidence have shown that apolipoprotein CIII (apoCIII) is a critical modulator of plasma TG metabolism mostly by inhibiting the hydrolysis of TG. Little is known about the role of apoCIII contained in high density lipoprotein (HDL) in plasma TG metabolism after a meal. Methods Fasting and 4-hour postprandial peripheral venous blood were collected in 91 subjects selected from our hospital. Serum lipid parameters, apoCIII levels and HDL subcomponents were tested by standard laboratory procedures, ELISA, and nuclear magnetic resonance (NMR), respectively. The t -test, and Non-parametric tests were performed to examine differences between groups, Pearson’s correlation and multiple regression analysis were used to assess the correlations between apoCIII (HDL-associated or nonHDL-associated) and postprandial TG. Results There was a significant increase in TG after a meal compared to fasting status [155.40(96.70–251.07) mg/dl .vs. 118.53(83.38–173.29)mg/dl, p < 0.001]. However, the total apoCIII levels were unchanged before (11.56(7.89–16.22) mg/dl) and after a meal (11.66(7.75–16.02)mg/dl, p = 0.124), while a significant increase in HDL-associated apoCIII (HDL-apoCIII) was observed from fasting (5.25(3.92–7.83)mg/dl) to post-meal (6.46(4.57–8.76)mg/dl, p = 0.001). Unlike nonHDL-apoCIII, HDL-apoCIII was positively correlated with both fasting and postprandial plasma TG in subjects with baseline plasma TG > 118.53 mg/dl (R = 0.503, p < 0.001 for fasting, R = 0.584, p < 0.001 for postprandial). Besides, in the subjects who had an abnormal TG response to a meal, which was defined as postprandial plasma TG increase of>30% compared to baseline TG levels, postprandial HDL-apoCIII was also increased significantly [5.37(3.52–7.02)mg/dl .vs. 6.64(4.61–8.86)mg/dl, p = 0.001]. The enrichment of apoCIII in HDL led to changes of TG, cholesterol, free cholesterol, phospholipid and apoAII contents in HDL particles defined by NMR. Conclusion Enrichment of apoCIII in HDL particles potentially plays an independent role in postprandial hypertriglyceridemia.
研究领域:
心脏病学
·
脂质代谢
·
临床生物化学
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Xiaoqing Wang,Weiyi Zhang,Junhua Shao,Xinglian Xu,Xue Zhao
The vegetable oils with varied chain length are commonly incorporated as protein-stabilized emulsion droplets to replace fats in the emulsified meat products. In this paper, we explored the effects of oil chain length (OCL) on the interfacial behaviour of myofibrillar protein (MP) and emulsion stability. The long chain triglyceride (LCT, soybean oil in this research) and medium chain triglyceride (MCT), which possessed similar polarity, were mixed in different volume ratios as model oils. The results suggested that the longer chain oil could impose a stronger hydrophobic effect on the protein, which promoted the structural unfolding and intermolecular interaction of interfacial MP. This contributed to the formation of a stiffer interfacial film (about 26.2 mN/m), which provided sufficient protection against coalescence. While on the other hand, the MP diffused faster towards the shorter chain interface (0.58 ± 0.1 mN m −1 s −0.5 ), leading to a fast decrease of interfacial tension and a larger magnitude of interfacial pressure (about 10.3 mN/m) herein. Therefore, with the extension of storage time, the shorter chain oil emulsion possessed smaller droplets (D 3,2 = 7.3 ± 0.4 μm; D 4,3 = 26.3 ± 1.3 μm) and creaming rate (14.5 ± 0.2%), which was overall more stable against macroscopic phase separation.
研究领域:
食品科学
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生物化学
·
界面化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Qingqing Cao,Jiaxin Chen,Qian Zhang,Xiaoxue Li,Yuqin Feng,Jiajun Guo,Mei Guo,Guishan Liu,Jingyuan Cai,Yunlong Zhao
The warmed-over flavor (WOF) can significantly impair the flavor quality of prepared foods, leading to reduced consumer acceptance. This study investigates the molecular composition of WOF and the alterations in lipid molecules during the heating-cooling-reheating process of bowl-steamed Tan lamb, employing a comprehensive analytical approach. By integrating GC–MS and GC-IMS analyses, 11 WOF-related compounds were identified, including furan, 2-pentyl-, and 2-pentanone. UPLC-MS/MS analysis revealed that phospholipids and triglycerides, particularly TG 49:4; O and PG 34:2, play a critical role in the formation and retention of WOF. These lipids exhibited a negative correlation with WOF, indicating that lipid degradation is the primary cause of WOF formation. The results of this study provide a theoretical foundation for the targeted inhibition of WOF in prepared foods.
研究领域:
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHARMACEUTICAL BIOLOGY
·
2017
·
IF 1.916
Xin Yang,Qian Wang,Zeng-run Pang,Meng-ran Pan,Wen Zhang
Context: Flavonoid-enriched extract from Hippophae rhamnoides L. (Elaeagnaceae) seed (FSH) has shown beneficial effects in anti-hypertension and lowering cholesterol level. However, evidence for its efficacy in treating obesity is limited.Objective: We sought to determine if FSH can reduce body weight and regulate lipid metabolism disorder in high fat diet (HFD)-induced obese mouse model, and to investigate potential molecular targets involved.Materials and methods: C57BL/6 mice were fed with HFD for 8 weeks to induce obesity. The modeled mice were divided into four groups and treated with vehicle, rosiglitazone (2 mg/kg), low (100 mg/kg) and high (300 mg/kg) dose of FSH, respectively. Normal control was also used. The treatments were administered orally for 9 weeks. We measured the effect of FSH on regulating body weight, various liver and serum parameters, and molecular targets that are key to lipid metabolism.Results: FSH administration at 100 and 300 mg/kg significantly reduced body weight gain by 33.06 and 43.51%, respectively. Additionally, triglyceride concentration in serum and liver were decreased by 15.67 and 49.56%, individually, after FSH (300 mg/kg) treatment. Upon FSH (100 and 300 mg/kg) treatment, PPARα mRNA expression was upregulated in liver (1.24- and 1.42-fold) and in adipose tissue (1.66- and 1.72-fold). Furthermore, FSH downregulated PPARγ protein level both in liver and adipose tissue. Moreover, FSH inhibited macrophage infiltration into adipose tissues, and downregulated TNFα mRNA expression in adipose tissue (38.01–47.70%).Conclusion: This effect was mediated via regulation of PPARγ and PPARα gene expression, and suppression of adipose tissue inflammation.
研究领域:
药理学
·
营养学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Xiaosan Wang,Cong Jiang,Wendi Xu,Zhicheng Miu,Qingzhe Jin,Xingguo Wang
Structured triacylglycerols (TAGs), rich in 1,3-dioleoyl-2-palmitoylglycerol (OPO) and 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL), were prepared by enzymatic acidolysis of fractionated palm stearin with free fatty acids . The effects of enzyme type, substrate molar ratio, enzyme load, temperature, and reaction time on the yields of OPO and OPL, palmitic acid (PA) content at the sn- 2 position of TAG, and the percentage of PA at the sn- 2 position relative to total PA content were investigated. Under optimal reaction conditions (palm stearin/oleic acid/linoleic acid molar ratio of 1:8:4, lipase NS 40086 loading of 8%, 60 °C, 4 h), the combined yields of OPO and OPL was 69.26%, PA content at the sn- 2 position reached 87.75%, and the relative percentage of PA at the sn- 2 position was up to 71.07%. The synthesized TAGs have potential to be used in infant formulas.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yiming Jiang,Shiqi Zhang,Linfan Pan,Juncai Leng,Tingyi Zhou,Mingxuan Liu,Li Li,Wei Zhao
Fat and its hydrolysis products, fatty acids, are indispensable nutritional components; however, prolonged excessive fat consumption, particularly in western diets, contributes to the onset of obesity and multiple metabolic disorders . In this study, we propose a daily-ingestible hydrogel (denoted as β C-MA hydrogel) composed of natural β -glucan and sodium carboxymethylcellulose crosslinked by malic acid at 120 °C. This hydrogel exhibits rapid swelling performance, up to 24-fold within 1 min and 176-fold after 1 h in deionized water . It also lengthens gastric retention and increases endogenous satiety signal levels, potentially controlling appetite and reducing food intake. Furthermore, β C-MA hydrogels that enter the small intestine can effectively inhibit fat hydrolysis and decrease triglyceride synthesis and transport. Specifically, the hydrogels inhibit the release of free fatty acids (FFAs) by approximately 50 % during digestion, influence the translocation of triglycerides and FFAs across the intestinal epithelium, and reduce the serum triglyceride levels by 22.2 %. These findings suggest that β C-MA hydrogels could serve as a noninvasive gastrointestinal device for weight control, with the advantage of reducing food intake and restoring lipid metabolism homeostasis.
研究领域:
营养学
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生物医学工程
·
代谢研究
✓ 已验证
DOI
PubMed
高影响因子
Food Science & Nutrition
·
2018
·Vol. 6
·期 8
·2020-2027
·
IF 1.521
Hong Fu,Mengqi Li,Ruimin Ni,Yangming Martin Lo
Two different fish oil preparations, namely triglycerides and ethyl esters containing, respectively, 30.02% and 74.38% of omega-3 fatty acids, were employed as the substrates for transesterification. Catalyzed by immobilized lipase using imidazolium-based ionic liquid systems, the total content of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the resulting triglyceride reached 63.60% when 4% hydrophobic ionic liquid was used, which was 11.74% higher than that of the triglyceride produced in a solvent-free reaction system. The activation energy of the product (triglyceride-type fish oil) was 173.64 KJ mol −1 , which was not significantly different from that of the commercial ethyl ester-type fish oil, so were the other thermal oxidative kinetic parameters. The kinetic parameters depicting the thermal and oxidative stability of the fish oil product provide the basis for industrial processing, storage, and applications.
研究领域:
生物化学
·
可持续生产
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Xin-Yun Wang,Jing Xie,Xin-Jun Chen
This study aimed to investigate the lipid oxidation and distribution in Bigeye tuna stored at 0 °C and 4 °C for 6 days. Tuna were evaluated by determining the peroxide value (POV), acid value (AV), anisidine value (AnV), polyene index, fluorescence ratio (FR), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI) content, and major glycerophospholipid molecular species. The value of lipid oxidation indexes (POV, AV, AnV, FR, PC, PE and PI) increased as the storage time increased. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) results indicated that the major types of lipids included diacylglycerol (DAG), monoacylglycerol (MAG), phospholipid (PL), and triacylglycerol (TAG). At least 136 PC and 64 PE molecular species were identified in Bigeye tuna. The results of the confocal laser scanning microscope analysis indicated the distribution of TAG and PL particles. In addition, principal component analysis showed that the contents of PI and TAG were positively correlated with PC, polyene index and lipid content but negatively correlated with PI, POV, FR, AOV, AnV, MAG, and DAG, which might be explained by distinguishing the lipid parameters affecting lipid oxidation. Therefore, this study may provide a novel method to evaluate lipid changes and contribute to the balanced nutritional value of aquatic foods during cold storage.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Weibo Zhang,Chong Chen,Yixuan Li,Fengyu Guo,Weiqian Liu,Siyuan Liu,Yanan sun,Xifan Wang,Yuemin Shen,Pengjie Wang
Stir-fried pepper tallow is widely used in cooking due to its special flavor, particularly in hot-pot dishes. However, the composition and source of the key aroma compounds in stir-fried pepper tallow are poorly understood, resulting in uneven quality. Here, the key aroma compounds were screened using flavor dilution factors (FD) and odor activity values (OAVs). A total of 41 odorants compounds were identified. Of these, 20 compounds with FD ≥ 8 were aroma-active compounds. Furthermore, among these 20 compounds, 15 with OAVs ≥ 1were the key aroma-active compounds and most of these (13 out of 15 odorants) were produced from pepper. Glycosides in pepper are the precursors of the most of these key aroma compounds. It may be possible to improve the flavor quality of stir-fried pepper tallow by hydrolyzing glycosides. These findings should help to establish a standard to assess and improve the quality of stir-fried pepper tallow.
研究领域:
食品科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 7
·1654
·
IF 3.098
Xiaozhi Qiao,Yunlan Li,Jiaqi Mai,Xiaoqing Ji,Qingshan Li
Dibutyltin dilaurate (DBTD) has multiple applications in daily life. However, DBTD is easily deposited in the liver and affects liver functions. This study was designed to explore the effects of DBTD on triglyceride metabolism in human normal hepatocyte HL7702 cells. Our results showed that the intracellular fat contents were dose-dependently decreased by DBTD. The expression of lipolysis genes and proteins were elevated while the lipogenesis genes and proteins were diminished by DBTD. The phosphorylation levels of ribosomal S6 kinase 1 were reduced by both rapamycin and DBTD, indicating that the mTOR pathway was suppressed possibly. The decreased sterol regulatory element-binding protein 1C (SREBP1C) transcription levels, as well as the increased peroxisome proliferator-activated receptor alpha (PPARα) transcription levels, caused by rapamycin and DBTD corresponded to the inactive mTOR pathway. In conclusion, it was possible that DBTD reduced the intracellular triglyceride through depressing the mTOR pathway and affecting its downstream transcription factors.Keywords:dibutyltin dilaurate (DBTD);triglycerides metabolism;mTOR pathway;SREBP1C;PPARα
研究领域:
毒理学
·
细胞生物学
·
代谢研究
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）