JOURNAL OF BIOLOGICAL CHEMISTRY
·
2020
·Vol. 295
·期 19
·6425-6446
·
IF 4.238
Chengmeng Jin,Xiaobing Zhu,Hao Wu,Yuqi Wang,Xun Hu
Phosphoglycerate kinase 1 (PGK1) plays important roles in glycolysis, yet its forward reaction kinetics are unknown, and its role especially in regulating cancer cell glycolysis is unclear. Here, we developed an enzyme assay to measure the kinetic parameters of the PGK1-catalyzed forward reaction. The Km values for 1,3-bisphosphoglyceric acid (1,3-BPG, the forward reaction substrate) were 4.36 μm (yeast PGK1) and 6.86 μm (human PKG1). The Km values for 3-phosphoglycerate (3-PG, the reverse reaction substrate and a serine precursor) were 146 μm (yeast PGK1) and 186 μm (human PGK1). The Vmax of the forward reaction was about 3.5- and 5.8-fold higher than that of the reverse reaction for the human and yeast enzymes, respectively. Consistently, the intracellular steady-state concentrations of 3-PG were between 180 and 550 μm in cancer cells, providing a basis for glycolysis to shuttle 3-PG to the serine synthesis pathway. Using siRNA-mediated PGK1-specific knockdown in five cancer cell lines derived from different tissues, along with titration of PGK1 in a cell-free glycolysis system, we found that the perturbation of PGK1 had no effect or only marginal effects on the glucose consumption and lactate generation. The PGK1 knockdown increased the concentrations of fructose 1,6-bisphosphate, dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, and 1,3-BPG in nearly equal proportions, controlled by the kinetic and thermodynamic states of glycolysis. We conclude that perturbation of PGK1 in cancer cells insignificantly affects the conversion of glucose to lactate in glycolysis.
研究领域:
生物化学
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癌症生物学
·
细胞代谢
·
酶学
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产品关联
DOI
PubMed
高影响因子
Science Bulletin
·
2025
·
IF 21.1
Han-Yue Yang,Bin Sun,Xue Xiao,Xuan Ai,Rui-Bin Jiang,Pei Chen,Yu Chen
Glycerol, a byproduct of biodiesel production, can be efficiently converted into valuable chemicals through electrocatalytic reaction. In this study, platinum (Pt) nanocrystals decorated on bismuth oxide (Bi 2 O 3 ) nanosheets (PtBi DONS) with an optimal Pt:Bi ratio of 1:1 were successfully synthesized using a galvanic replacement method. Pt 1 Bi 1 DONS exhibit remarkable electrocatalytic performance for glycerol electrooxidation, achieving an excellent catalytic activity (current of 0.82 A mg Pt –1 at 0.67 V vs. reversible hydrogen electrode (RHE)) and an exceptional selectivity of 96.6% for C 3 products, particularly glyceric acid. The superior performance stems from two key factors: (1) electron transfer from Bi 2 O 3 to Pt creates electron-rich Pt sites that suppress C–C bond cleavage and (2) Bi 2 O 3 facilitates favorable glycerol adsorption configurations through multiple hydroxyl group interactions. Mechanistic studies using operando spectroscopy and electrochemical impedance spectroscopy revealed that the synergistic effect between Pt and Bi 2 O 3 promotes rapid charge transfer and stable intermediate formation. Moreover, PtBi DONS showed excellent versatility in oxidizing other polyols compared with monoalcohols.
研究领域:
化学工程
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science Bulletin
·
2019
·
IF 6.277
Junbo Zhang,Xiaolin Li,Ming Xu,Yusen Yang,Yinwen Li,Ning Liu,Xiaoyu Meng,Lifang Chen,Shuxian Shi,Min Wei
Glycerol (GLY) aerobic oxidation in an aqueous solution is one of the most prospective pathways in biomass transformation, where the supported catalysts based on noble metals (mainly Au, Pd, Pt) are most commonly employed. Herein, Pt nanoparticles supported on rehydrated Mg x Al 1 -hydrotalcite (denoted as re-Mg x Al 1 -LDH-Pt) were prepared via impregnation-reduction method followed by an in situ rehydration process, which showed high activity and selectivity towards GLY oxidation to produce glyceric acid (GLYA) at room temperature. The metal-support interfacial structure and catalyst basicity were modulated by changing the Mg/Al molar ratio of the hydrotalcite precursor, and the optimal performance was achieved on re-Mg 6 Al 1 -LDH-Pt with a GLY conversion of 87.6% and a GLYA yield of 58.6%, which exceeded the traditional activated carbon and oxide supports. A combinative study on structural characterizations (XANES, CO-FTIR spectra, and benzoic acid titration) proves that a higher Mg/Al molar ratio promotes the formation of positively charged Pt δ + species at metal-support interface, which accelerates bond cleavage of α-C–H and improves catalytic activity. Moreover, a higher Mg/Al molar ratio provides a stronger basicity of support that contributes to the oxidation of terminal-hydroxyl and thus enhances the selectivity of GLYA. This catalyst with tunable metal-support interaction shows prospective applications toward transformation of biomass-based polyols.
研究领域:
化学工程
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催化科学
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生物质转化
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DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2019
·Vol. 21
·期 16
·4434-4442
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IF 9.405
Teng Li,Shengqin Liu,Bing Wang,Jingen Long,Jun Jiang,Ping Jin,Yao Fu,Haizhu Yu,Weiran Yang
Glycerol, generated in abundance as the by-product in the process of biodiesel production and saponification, has seen attempts to convert it into value-added chemicals. However, due to the low selectivity of hydrogenolysis of the secondary hydroxyl group, valuable 1,3-substituted chemicals are difficult to obtain from glycerol by chemocatalysis. In this work, glyceric acid (GA), a renewable biomass from glycerol, was quantitatively converted to 3-iodopropionic acid (3-IPA) at 373 K in 3 h by hydroiodic acid mediated hydrogenation. As the reductant in this process, HI is oxidized to I2 and then regenerated in situ by metal catalysts and H2. The reaction pathway was proposed by intermediate identification and verified by a kinetics study and computational method. The catalytic system was shown to be stable and can be reused several times without loss in activity. As a 1,3-substituted chemical, 3-IPA is not only a potential monomer to form poly-3-hydroxypropionic acid, but also a good platform molecule to produce useful chemicals, e.g. 3-hydroxypropionic acid (3-HPA) and acrylic acid (AA), due to its highly reactive nature.
研究领域:
化学工程
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可再生资源化学
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有机合成
✓ 已验证
产品关联
DOI
高影响因子
AICHE JOURNAL
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2022
·Vol. 68
·期 12
·e17852
·
IF 4.167
Yang Zhao,Xinyi Zhang,Jiarui Yang,Mingyu Gao,Pengfei Yang,Qian Wang,Dianqing Li,Junting Feng
In this work, a commercial P25 (TiO 2 ) with phase junctions between (101) and (110) faces supported Pt catalyst was prepared for glycerol selective oxidation, giving a decent 76.8% glyceric acid (GLYA) yield, and good stability. The kinetic experiments calculated apparent activation energy, turnover frequency (TOF), and reaction rates, demonstrating Pt/P25 could facilitate glyceraldehyde (GLYAD) formation and inhibit GLYA over-oxidation. Series of structural techniques confirmed the 101/110 junctions in P25 contributed electronic effect on Pt through metal-support interface, which improved the oxygen activation ability and optimized the GLYAD/GLYA adsorption modes. Furthermore, progressive scale-up reactions were tested and the mass transfer limitations under different conditions were thoroughly studied. The obtained mass transfer factors suggested the gas–liquid and liquid–solid mass transfer were severely limited. Finally, through optimizing the conditions, Pt/P25 catalyst achieved 75.8% GLYA yield at 300 ml-scale reaction, realizing equivalent magnification with the 5 ml-scale experiment.
研究领域:
催化化学
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材料科学
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反应动力学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROSCOPY LETTERS
·
2014
·
IF 0.718
Rui-Yong Wang,Jing Wu,Lv-Jing Wang,Rui Wang,Huan-Jing Dou
Water-soluble and stable CdTe quantum dots were synthesized in aqueous solution with thioglycolic acid as the stabilizer. A spectrofluorometric method for the determination of iron (II) has been developed based on quenching of the fluorescence of CdTe quantum dots by iron (II) in aqueous solutions. It can perform an accurate and simple determination of iron (II) concentration in water samples. Under optimum conditions, the quenched fluorescence intensity increased linearly with the concentration of iron (II) ranging from 5.0 × 10−8 to 4.0 × 10−6 mol/L with a correlation coefficient R = 0.9969. The limit of detection for iron (II) was found to be 1.2 × 10−8 mol/L. As an application, the proposed method was successfully applied to the analysis of iron (II) in water samples, and the results were satisfactory.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
GREEN CHEMISTRY
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2022
·Vol. 24
·期 19
·7644-7651
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IF 11.034
Linfeng Zhang,Changpo Ma,Chenyu Wang,Guangyu Sun,Yongqi Shu,Wenya Zou,Tao Yang,Weiran Yang
Glyceric acid (GA), a glycerol derivative, can be used as a versatile platform molecule to produce value-added chemicals. Succinic acid (SA) is a very important bio-based molecule that acts as a fine chemical and is used in biodegradable polymer synthesis. It has been shown earlier that GA can be effectively converted to the platform chemical 3-iodopropionic acid (3-IPA) with a RhCl3/HI catalyst system. In this work, we first explored the production of SA from 3-IPA by carbonylation. With RhCl3 as the catalyst and acetic acid (AA) and water as the solvent, as high as 78% yield of SA can be achieved. Considering the similarity of the catalysts used in two steps, we then further probed the one-step synthesis of SA directly from GA. Under optimized reaction conditions, 71% yield of SA can be obtained directly from GA by reductive carbonylation with RhCl3 and I2 as the catalyst under H2 and CO atmospheres. This is the first report of the direct conversion of GA to SA, which provides a novel method for glycerol valorization.
研究领域:
化学工程
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催化
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生物化工
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
An-Ni Zheng,Zhao-Yun Wang,Jia-Qiang Yang,Lei Jin,Fang-Zu Yang,Dong-Ping Zhan
Advanced Ag nanoparticles (Ag NPs) were prepared by wet chemical oxidation–reduction method, using mainly the tannic acid as reducing agent and carboxymethylcellulose sodium as stabilizer . The prepared Ag NPs uniformly disperse and are stable for more than one month without agglomeration. The studies of transmission electron microscopy (TEM) and ultraviolet–visible (UV–vis) absorption spectroscopy indicate that the Ag NPs are in homogeneous sphere with only 4.4 nm average size and narrow particle size distribution . Electrochemical measurements reveal that the Ag NPs behave excellent catalytic activity for electroless copper plating using glyoxylic acid as reducing agent. In situ fourier transform infrared ( in situ FTIR) spectroscopic analysis combined with density functional theory (DFT) calculation illustrate that the molecular oxidation of glyoxylic acid catalyzed by Ag NPs is as the following routes: glyoxylic acid molecule first is adsorbed on Ag atoms with carboxyl oxygen terminal, then hydrolyzed to diol anionic intermediate, and last oxidized to oxalic acid . Time-resolved in situ FTIR spectroscopy further reveals the real-time reactions of electroless copper plating as follows: glyoxylic acid is continuously oxidized to oxalic acid and releases electrons at the active catalyzing spots of Ag NPs, and Cu(II) coordination ions are in situ reduced by the electrons. Based on the excellent catalytic activity, the advanced Ag NPs can replace the expensive Pd colloids catalyst and successfully apply in through-holes metallization of printed circuit board (PCB) by electroless copper plating.
研究领域:
材料科学
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电化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Mengzhao Jiao,Zhengjun Li,Xianglai Li,Zijie Zhang,Qipeng Yuan,Frank Vriesekoop,Hao Liang,Juewen Liu
Self-inactivation of oxidases due to by-product hydrogen peroxide (H 2 O 2 ) is a main obstacle to applying these enzymes in chemical production. Herein, we report an efficient cascade strategy of co-immobilizing a natural oxidase and catalase-mimicking coordination nanoparticles. Fe 3+ and adenosine monophosphate (AMP) formed coordination nanoparticles with comparable performance as natural catalase at ambient conditions for degrading H 2 O 2 , but the former still maintained about 90% or 75% catalytic activity after pH 2.0 or 90 °C treatment, respectively. Upon polymerization with a few acrylic monomers, glycolic acid oxidase (GyAO) was co-immobilized with Fe 3+ /AMP within a nanogel. Benefiting from the confinement and protection effect of the nanogel, the catalytic activity increased by 5-fold at pH 4, and 10-fold at 80 °C, and the catalytic activity of the nanogel still retained more than 65% after recycling 20 times. More importantly, the tolerance of the nanogel to glycolic acid enhanced 100 times compared with the free GyAO enzyme. This study has expanded the application of nanozymes to industrial catalysis, where harsh conditions are expected.
研究领域:
生物化学
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工业酶学
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纳米材料科学
✓ 已验证
DOI
高影响因子
ChemCatChem
·
2022
·Vol. 14
·期 16
·e202200509
·
IF 5.497
Jiefei Li,Zhenyu Li,Zexuan Zheng,Xueqiong Zhang,Hao Zhang,Hang Wei,Haibin Chu
Graphical Biomass conversion : Electro-oxidation of glycerol over the Pt/CNT catalyst suffers from the poor activity, low glyceric acid (GLYA) selectivity, and high cost of the noble metal. The incorporation of CeO 2 into Pt/CNT achieves much enhanced yield of GLYA with even half dosage of Pt. Electrocatalytic oxidation of glycerol is a highly efficient way for upgrading glycerol toward fine chemicals. Currently, electro-oxidation of glycerol into the high value-added C3 products remains a big challenge because of the poor catalytic activity, low selectivity, and high cost of the noble metal catalysts, especially in the alkaline medium. Here, we report that the Pt−CeO 2 /CNT (CNT=carbon nanotubes) catalyst remarkably promotes the conversion (86.5 % vs. 37.6 %) and recycling stability for glycerol electro-oxidation reaction, as compared with Pt/CNT. However, the improved catalytic activity of Pt−CeO 2 /CNT is accompanied by the further oxidation of C3 products including glyceric acid (GLYA). By tuning the product distribution by the ratio of CeO 2 and CNT, applied potential, and the Pt loading amount, the high selectivities of C3 products (95 %) and GLYA (59 %) are achieved together with the high glycerol conversion (86 %), leading to the impressive yields of C3 products (81 %) and GLYA (50 %). The unique contributions of CeO 2 includes not only the enhanced catalytic activity and recycling stability, but also the suppressed further oxidation of GLYA. Moreover, higher yield of GLYA is reached over Pt−CeO 2 /CNT even with half loading of Pt, as compared with Pt/CNT.
研究领域:
化学工程
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催化材料科学
✓ 已验证
产品关联
DOI
高影响因子
npj Precision Oncology
·
2025
·Vol. 9
·期 1
·1-13
·
IF 8
Wang Zexuan,Guo Yaoyu,Hu Kefei,He Tingjiang,Qin Tong,Zhang Ludan,Xu Fang,Xu Yuanzhi,Cheng Mingjiao,Zhang Jintao,Zhao Qianwei
Phosphoglycerate mutase 2 (PGAM2) is a crucial glycolytic enzyme. Recently, we have found that both the protein and acetylation levels of PGAM2 are down-regulated in hepatocellular carcinoma (HCC) tissues. However, the functional significance of PGAM2 in HCC progression remains poorly characterized. In this study, we demonstrated that PGAM2 functioned as a tumor suppressor in HCC progression, and knockdown of PGAM2 promoted proliferation of HCC cells and tumor growth both in vitro and in vivo. Moreover, we identified lysine 100 (K100) in PGAM2 as the predominant deacetylation site of sirtuin-2 (SIRT2), and that deacetylation of K100 destabilized PGAM2 by promoting its ubiquitination and degradation. Importantly, we discovered that PGAM2 suppressed aerobic glycolysis through an enzymatic activity-independent mechanism in HCC cells. Mechanistic investigations revealed that PGAM2 knockdown upregulated lactate dehydrogenase A (LDHA) expression via activation of the signal transducer and activator of transcription 3 (STAT3). Furthermore, we found that knockdown of PGAM2 sensitized HCC cells to sorafenib treatment. In conclusion, these findings elucidate the tumor-suppressive role of PGAM2 in HCC progression and its post-translational regulation through SIRT2-mediated deacetylation, which provide novel biomarkers and therapeutic targets for HCC treatment.
研究领域:
肿瘤学
·
分子生物学
·
代谢研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2021
·
IF 5.221
Cheng Haijun,Wei Jiangli,Liang Min,Dai Suyi,Liu Xiongmin,Ma Li,Wang Hongyun,Lai Fang
Abstract: The synthesis costs of macrolide musks are higher than those of other commercial musks. To make this process less expensive, eggshell waste was calcined at a low temperature to obtain a catalyst for the cyclopentadecanolide production via reactive distillation using a glycerol entrainer. X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy analyses of the original and recovered catalysts revealed that the main catalytic ingredient was calcium glycerolate (CaG) and not calcium diglyceroxide (CaDG). The basic strengths of CaG and CaDG obtained by Hammett indicators were 7.2 < H_≤ 15.0 and 9.8 < H_≤15.0, while the corresponding base amounts were 1.9 and 7.3 mmol/ g, respectively. Because CaG was soluble in glycerine, the catalyst was efficiently reused. The reaction product containing over 95.0% cyclopentadecanolide with a yield of 49.8% was obtained at a temperature of 190°C and catalyst amount of 12 wt% after 7 h of reaction. Thus, eggshell waste may be directly placed into the reaction mixture after calcination at 600°C to synthesise a large amount of cyclopentadecanolide within a relatively short time. The results of this work indicate that eggshell waste can serve as a potential eco-friendly and affordable catalyst source for the production of macrolide musks.
研究领域:
化学工程
·
环境科学
·
有机合成
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）