Fermentation-Basel
·
2023
·Vol. 9
·期 9
·789
·
IF 3.7
William Rodrigues Alves,Thiago Alessandre da Silva,Arion Zandoná Filho,Luiz Pereira Ramos
This work aimed at producing lactic acid (LA) from sugarcane bagasse after steam explosion at 195 °C for 7.5 and 15 min. Enzymatic hydrolysis was carried out with Cellic CTec3 and Cellic HTec3 (Novozymes), whereas fermentation was performed withBacillus coagulansDSM2314. Water washing of pretreated solids before enzymatic hydrolysis improved both hydrolysis and fermentation yields. The presence of xylo-oligosaccharides (XOS) in substrate hydrolysates reduced hydrolysis efficiency, but their effect on fermentation was negligible. The presence of fermentation inhibitors in C5 streams was circumvented by adsorption on activated carbon powder with no detectable sugar losses. High carbohydrates-to-LA conversions (Yp/s) of 0.88 g·g−1were obtained from enzymatic hydrolysates of water-washed steam-exploded materials that were produced at 195 °C, in 7.5 min, and the use of centrifuged-but-never-washed pretreated solids decreased Yp/sby 16%. However, when the detoxified C5 stream was added at a 10% ratio, Yp/swas raised to 0.93 g·g−1for an LA productivity of 2.55 g·L−1·h−1. Doubling the pretreatment time caused a decrease in Yp/sto 0.78 g·g−1, but LA productivity was the highest (3.20 g·L−1·h−1). For pretreatment at 195 °C for 7.5 min, the elimination of water washing seemed feasible, but the use of longer pretreatment times made it mandatory to eliminate fermentation inhibitors.Keywords:sugarcane bagasse;steam explosion;enzymatic hydrolysis;fermentation;lactic acid
研究领域:
生物技术
·
化学工程
✓ 已验证
DOI
高影响因子
Polymers
·
2018
·Vol. 10
·期 3
·282
·
IF 2.935
Qiang Zhang,Xueqin Zhang,Ziyan Zhu,Aiping Zhang,Chunhui Zhang,Xiaoying Wang,Chuanfu Liu
Esterification is a versatile way to produce the derivatives of lignocellulose with developed properties. However, the traditional heterogeneous esterification of lignocellulose suffered from the drawbacks of low efficiency, additional reaction medium and heating. In the present study, an efficient method was developed to produce the functionalized sugarcane bagasse (SCB) by ball milling without any additional solvents and heating. The effects of pulverization time, rotation speed, the kind of linear chain anhydrides, the ratio of anhydrides to SCB, with or without pyridine catalyst and the dosage of catalyst were investigated on weight percent gain (WPG) of SCB esters. The results indicated that the high efficiency of this mechanocatalystic esterification was probably due to the destroyed crystalline structure and the promoted penetration of the esterifying reagent onto SCB bulk caused by ball milling. The maximumWPGof SCB acetate, propionate and butyrate reached 33.3%, 33.6% and 32.4%, respectively. The physicochemical structure of the esterified SCB was characterized with Fourier transform infrared spectra (FT-IR), solid state cross-polarized magic angle spinning13C nuclear magnetic resonance (CP/MAS13C-NMR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The direct evidence of the esterification occurrence was provided with FT-IR and solid-state CP/MAS13C-NMR. The thermal stability of SCB increased upon the mechanocatalytic esterification. The results implied that the relatively homogeneous modification was achieved with this semi-homogeneous esterification method by ball milling.Keywords:sugarcane bagasse;esterification;ball milling;bio-based materials
研究领域:
生物质化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2025
·
IF 5.2
Zi-Quan Wang,Ya-Peng Du,Xiao-Ping Zheng,Yu Chai,Yu-Cang Zhang,Yan-Zhen Zheng
The direct conversion of raw lignocellulosic waste into specialty chemicals such as levulinic acid (LA) has the potential to significantly enhance biorefinery profitability. Here, we demonstrated that incorporating γ-valerolactone (GVL) as a co-solvent during the SO 3 H-functionalized ionic liquid-catalyzed conversion of bagasse in water markedly enhanced reaction rates and catalytic activity, achieving a LA yield as high as 76.1 % with a 1:1 vol ratio of GVL to water. This catalytic system also effectively converted other lignocellulosic waste to LA. Reaction constants and activation energies for the bagasse dehydration process to LA were obtained. Comprehensive characterization via Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) elucidated significant changes in functional groups, surface morphology, and crystallinity index of solid residues over extended reaction times. Notably, hemicellulose and cellulose were converted within 4 h, with lignin remaining in the solid residues and forming by-products after prolonged reaction times. This efficient one-pot conversion method showed promise as a sustainable approach to transform agricultural waste into LA, offering new opportunities for eco-friendly resource utilization.
研究领域:
生物化学
·
可再生能源
✓ 已验证
产品关联
DOI
高影响因子
BIORESOURCE TECHNOLOGY
·
2011
·
IF 4.365
Xian-wei Sui,Zhi Wang,Bing Liao,Ying Zhang,Qing-xiang Guo
Fast pyrolysis of bagasse pretreated by sulfuric acid was conducted in a fixed bed reactor to prepare levoglucosenone (LGO), a very important anhydrosugar for organic synthesis. The liquid yield and LGO yield were studied at temperatures from 240 to 350 °C and sulfuric acid loadings from 0.92 to 7.10 wt.%. An optimal LGO yield of 7.58 wt.% was obtained at 270 °C with a sulfuric acid pretreatment concentration of 0.05 M (corresponding to 4.28 wt.% sulfuric acid loading). For comparison, microcrystalline cellulose pretreated by 0.05 M sulfuric acid solution was pyrolyzed at temperature from 270 °C to 320 °C, and bagasse loaded with 3–5 wt.% phosphoric acid was pyrolyzed at temperature from 270 °C to 350 °C. The highest yield of LGO from bagasse was 30% higher than that from microcrystalline cellulose, and treatment with sulfuric acid allowed a 21% higher yield than treatment with phosphoric acid.
研究领域:
生物化学工程
·
可再生资源利用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2022
·
IF 8.91
Jiequan Liu,Ruyi Zhou,Junxia Yu,Li Guo,Xiaodi Li,Chunqiao Xiao,Haobo Hou,Ruan Chi,Guoqing Feng
Potentially toxic elements including lead (Pb), manganese (Mn), and copper (Cu) released from copper tailings would cause severe long-term environmental risks and potential threats to human health. To prevent these negative effects caused by the release of the metals, a novel magnetic carboxyl groups modified bagasse with high adsorption affinity and strong magnetism was synthesized through an in-situ precipitation method and used to simultaneously remove Pb, Mn, and Cu from the eluate of copper tailings. Results showed that release of Pb, Mn, and Cu from the copper tailings was pH, time, and particle size dependent, and maximum concentrations of them released in the eluate was 1.7, 1.9, and 4.1 mg L −1 under weak acid conditions. Batch adsorption experiment showed that the as-synthesized magnetic modified bagasse could selectively absorb Pb, Mn, and Cu from a complex solution with adsorption capacity of 137.3, 13.1, and 90.0 mg g −1 , respectively. X-ray photoelectron spectroscopy (XPS) and energy dispersive spectroscopy-mapping (EDS-mapping) demonstrated that Pb, Mn, and Cu interacted with the magnetic modified biosorbent mainly through coordination and ion exchange. Column experiments showed that higher than 99.5% of the released Pb, Mn, and Cu could be simultaneously removed by the magnetic modified bagasse, and the maximum concentrations of them released in the eluate of the copper tailings were all decreased to lower than 0.01 mg L −1 , which reached the discharge standards. After recycled by a magnet, the magnetic modified bagasse could be collected easily and used repeatedly. Because of the high efficiency and easy recovery, the used method had great practical application value in removal of potentially toxic elements released from metallic tailings.
研究领域:
环境科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sugar Tech
·
2021
·Vol. 24
·期 3
·832-840
·
IF 1.591
Yang Yujiao,Zheng Rui,Zhang Pingling,Wen Jing,Luo Yue,Zhao Zhengang,You Lijun,Ho Chi-Tang
Sugarcane is one of the most important crops in the world and rich in polyphenols. In this study, quercetin, apigenin, luteolin, tricin and p -coumaric acid, the major polyphenols in sugarcane, were used to evaluate the antiproliferative activities toward MCF-7 human breast cancer cells independently and in combination. These five polyphenols all showed inhibitory effects against MCF-7 cells within different dose ranges. The combinations of luteolin and three polyphenols (quercetin, apigenin, p -coumaric acid) were studied. p -Coumaric acid together with luteolin exhibited synergistic effects at all concentrations tested: the CI (Combination Index) values at 50%, 75% and 90% inhibition of MCF-7 cell growth were 0.68 ± 0.06, 0.70 ± 0.08 and 0.83 ± 0.09, respectively. Although the two-way combination of luteolin and apigenin showed moderate antagonistic effect (CI, 1.26 ± 0.24) at EC 50 , the CI value dropped as the inhibition rate increased, showing a synergistic effect at the inhibition rate above 70%. The combination of luteolin and quercetin indicated antagonistic effect at the inhibition rate above 50% (Fa 0.5, CI = 1.10 ± 0.05). p -Coumaric acid with quercetin exhibited antagonistic actions (Fa 0.5, CI = 1.62 ± 0.22) at all concentrations tested. In addition, the EC 50 values of luteolin and quercetin against MCF-7 cells decreased by 1.91 and 2.62-fold after combining with 20 µM tricin. The results provide evidence of synergistic relation among polyphenols present in sugarcane in inhibition of cancer cell growth and of their potential clinical application.
研究领域:
肿瘤学
·
植物化学
·
药理学
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2014
·Vol. 4
·期 1
·1-8
·
IF 5.078
Zhou Dinggang,Guo Jinlong,Xu Liping,Gao Shiwu,Lin Qingliang,Wu Qibin,Wu Luguang,Que Youxiong
To meet the demand for detection of foreign genes in genetically modified (GM) sugarcane necessary for regulation of gene technology, an efficient method with high specificity and rapidity was developed for the cry1Ac gene, based on loop-mediated isothermal amplification (LAMP). A set of four primers was designed using the sequence of cry1Ac along with optimized reaction conditions: 5.25 mM of Mg 2+ , 4:1 ratio of inner primer to outer primer, 2.0 U of Bst DNA polymerase in a reaction volume of 25.0 μL. Three post-LAMP detection methods (precipitation, calcein (0.60 mM) with Mn 2+ (0.05 mM) complex and SYBR Green I visualization), were shown to be effective. The sensitivity of the LAMP method was tenfold higher than that of conventional PCR when using templates of the recombinant cry1Ac plasmid or genomic DNA from cry1Ac transgenic sugarcane plants. More importantly, this system allowed detection of the foreign gene on-site when screening GM sugarcane without complex and expensive instruments, using the naked eye. This method can not only provide technological support for detection of cry1Ac , but can also further facilitate the use of this detection technique for other transgenes in GM sugarcane.
研究领域:
分子生物学
·
植物生物技术
·
基因工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2017
·
IF 5.651
Hongdan Zhang,Shuaishuai Zhang,Hongyou Yuan,Gaojin Lyu,Jun Xie
An organosolv pretreatment system consisting of 60% ethanol and 0.025 mol·L −1 FeCl 3 under various temperatures was developed in this study. During the pretreatment, the highest xylose yield was 11.4 g/100 g raw material, representing 49.8% of xylose in sugarcane bagasse. Structural features of raw material and pretreated substrates were characterized to better understand how hemicellulose removal and delignification affected subsequent enzymatic hydrolysis. The 160 °C pretreated solid presented a remarkable glucose yield of 93.8% for 72 h. Furthermore, the influence of different additives on the enzymatic hydrolysis of pretreated solid was investigated. The results indicated that the addition of Tween 80 shortened hydrolysis time to 6 h and allowed a 50% reduction of enzyme loading to achieve the same level of glucose yield. This work suggested that FeCl 3 -catalyzed organosolv pretreatment could improve the enzymatic hydrolysis significantly and reduce the hydrolysis time and enzyme dosage with the addition of Tween 80.
研究领域:
生物技术
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 19
·3134
·
IF 5.561
Huahua Qiao,Liwei Chen,Jinsong Yang,Wenbo Zhi,Rong Chen,Tianyu Lu,Haisheng Tan,Zhanwu Sheng
Fermentation technology is of great significance for food preservation; through fermentation, while extending the shelf life of food, it can also improve the sensory quality of food and increase its nutritional value. Compared with natural fermentation, the use of specific microorganisms for fermentation can be used to determine the direction of fermentation. Therefore, in order to better explore the effect of bacterial community structure on the quality of sugarcane juice naturally fermented and inoculated with different lactic acid bacteria in the fermentation process, the most suitable method for sugarcane juice fermentation was found, which provided a theoretical basis for the safe production of fermented sugarcane juice. In this experiment, the sugarcane juice was treated differently and divided into four groups: natural fermentation, fermentation withLactobacillusHNK10, fermentation withLactococcusHNK21, and HNK10 + HNK21 compound fermentation. The changes in bacterial community structure of different treatments of sugarcane juice during fermentation were analyzed by high-throughput sequencing technology, and the quality change of different treatments of sugarcane juice during fermentation was analyzed by high-performance liquid chromatography, and the change in its bacteriostatic ability was explored. The results showed that after the sugarcane juice treated withLactobacillusHNK10 was fermented at 37 °C for 48 h, the final fermented sugarcane juice had a large abundance of lactic acid bacteria and high-quality and strong antibacterial activity. Conclusions: changes in the bacterial community structure during the fermentation of sugarcane juice affect the formation of organic acids and the change of bacteriostatic ability and directly determine the quality and shelf life of fermented sugarcane juice.Keywords:sugarcane juice;fermentation;high-throughput sequencing;high-performance liquid chromatography
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·Vol. 49
·期 9
·3783-3794
·
IF 2.5
Yan He,Tong Liu,Yuehua Deng
In this study, sugarcane bagasse biochar (SBBC) and its MgFe2O4 modified composites with different MgFe2O4/SBBC mass ratios were fabricated to remove norfloxacin (NOR) from aqueous solutions. The results showed that MgFe2O4@SBBC (1 : 2) prepared via impregnation coprecipitation exhibited excellent adsorption performance and environmental friendliness. The increased surface area, improved pore structure, abundant surface functional groups, and decreased pHpzc endowed MgFe2O4@SBBC (1 : 2) with a higher adsorption efficiency (16.9 mg g−1, 84.3%) than that of SBBC (9.76 mg g−1, 48.8%). The pseudo-second-order model better described the kinetic process, indicating that chemisorption mainly occurred on both biochars. Isotherm fitting results indicated that NOR was adsorbed on SBBC primarily by multilayer adsorption, whereas monolayer adsorption was observed for MgFe2O4@SBBC (1 : 2). The adsorption of NOR onto both biochars was spontaneous, endothermic, and highly pH-dependent. Combined with BET, FTIR and XPS results, NOR adsorption by SBBC was controlled by pore filling, hydrogen bonds, and π–π electron donor acceptor interactions. In addition to these mechanisms, electrostatic attraction and surface complexation jointly strengthened the adsorption capacity of MgFe2O4@SBBC (1 : 2).
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Phytopathology Research
·
2024
·Vol. 6
·期 1
·1-15
·
IF 3.2
Li Xiufang,Liu Zongling,Wu Haoming,Yu Zhuoxin,Meng Jiaorong,Zhao Haiyun,Deng Xingli,Su Yizu,Chen Baoshan,Li Ru
Sugarcane ( Saccharum spp.) is a major sucrose and bioenergy crop in the world. The fungal pathogen Sporisorium scitamineum causes sugarcane smut, a devastating disease that destroys stalks and reduces sugar content in sugarcane. This disease can be controlled most effectively by applying smut-resistant sugarcane varieties. Previous studies have shown that Dirigent (DIR ) genes are involved in the synthesis of the lignin precursor pinoresinol, which plays a crucial role in plant resistance to biotic stresses. However, the immune response of the DIR homologs in sugarcane ( ScDIR ) has not been reported yet. In this study, we found that the lignin content of smut-resistant sugarcane varieties (ZZ1, ZZ6, and ZZ9) was significantly higher than that of smut-susceptible varieties (GT42, ROC22, and FN41), and the lignin content of sugarcane increased after smut infection. The smut-resistant and smut-susceptible clones derived from the same genetic population (ROC25 × YZ89-7) showed similar patterns. Quantitative real-time PCR assays revealed that among the 64 DIR genes in sugarcane, ScDIR5 , ScDIR7 , ScDIR11 , and ScDIR40 showed elevated expression after S. scitamineum infection. In vitro coupling reactions showed that the four corresponding ScDIR proteins could mediate the coupling of coniferyl alcohol and its conversion into the lignin precursor pinoresinol. Overexpression of the four ScDIR genes in Nicotiana benthamiana enhanced disease resistance to the fungal pathogens Sclerotium rolfsii , Rhizoctonia solani , and Botrytis cinerea . Moreover, transgenic sugarcane overexpressing these ScDIR genes showed enhanced resistance to smut disease. Taken together, our findings provide evidence that sugarcane ScDIR genes can improve the resistance of plants to fungal pathogens and highlight their potentials in sugarcane breeding for disease resistance.
研究领域:
植物病理学
·
分子生物学
·
遗传学
·
农业科学
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Manman Xu,Anqi Wang,Ying Xiang,Ahsan Ejaz,Junfeng Niu
Developing the unique structure of biomass as a template is beneficial for promoting the commercial application of template-based hierarchical porous carbon materials. Herein, self-template bagasse-based porous carbons with high capacitance performance are successfully synthesized though low-temperature carbonization to fix the original vascular bundle structure of the bagasse and high-temperature activated carbonization to obtain hierarchical porous structures in the tube wall. As electrode materials for supercapacitors, the as-fabricated self-template porous carbons display a high specific surface of 1351.7 m 2 g −1 , abundant oxygen functional groups of 17.33%, an ultrahigh specific capacitance of 418.5 F g −1 at 0.5 A g −1 and a good rate performance of 63.1% at a current density ranging from 0.5 to 10 A g −1 , which is mainly attributed to both natural and synthetic hierarchical porous structures in the obtained samples. Moreover, the self-template bagasse-based porous carbon devices also possess a remarkable energy density of 14.5 Wh kg −1 at 125 W kg −1 and good cycling stability of up to 98.9% after 10,000 cycles at 2 A g −1 . The design strategy described in this work offers a method to further simplify the template synthesis steps, while improving the performance, of biomass-based hierarchical porous carbons. Therefore, these materials can be developed as high capacitance electrodes for energy storage applications.
研究领域:
材料科学
·
能源存储
·
生物质利用
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）