GLYCOCONJUGATE JOURNAL
·
2016
·Vol. 33
·期 2
·159-168
·
IF 1.828
Liu Fang F.,Kulinich Anna,Du Ya M.,Liu Li,Voglmeir Josef
Two putative α-mannosidase genes isolated from the rather unexplored soil bacterium Solitalea canadensis were cloned and biochemically characterised. Both recombinant enzymes were highly selective in releasing α-linked mannose but no other sugars. The α-mannosidases were designated Sca2/3Man2693 and Sca6Man4191, and showed the following biochemical properties: the temperature optimum for both enzymes was 37 °C, and their pH optima lay at 5.0 and 5.5, respectively. The activity of Sca2/3Man2693 was found to be dependent on Ca 2+ ions, whereas Cu 2+ and Zn 2+ ions almost completely inhibited both α-mannosidases. Specificity screens with various substrates revealed that Sca2/3Man2693 could release both α1-2- and α1-3-linked mannose, whereas Sca6Man4191 only released α1-6-linked mannose. The combined enzymatic action of both recombinant α-mannosidases allowed the sequential degradation of high-mannose-type N-glycans. The facile expression and purification procedures in combination with strict substrate specificities make α-mannosidases from S. canadensis promising candidates for bioanalytical applications.
研究领域:
微生物学
·
酶学
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生物化学
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DOI
PubMed
高影响因子
MOLECULES
·
2017
·Vol. 22
·期 10
·1598
·
IF 2.861
Jing Chen,Yuchao Chen,Yi Cheng,Youheng Gao
Mannose-diester lauric diacid-cholesterol (Man-DLD-Chol), as a liposomal target ligand, was synthesized by lipase catalyzed in a non-aqueous medium. Its chemical structure was confirmed by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. Glycyrrhetinic acid (GA) liposomes containing Man-DLD-Chol (Man-DLD-Chol-GA-Lp) were prepared by the film-dispersion method. We evaluated the characterizations of liposomes, drug-release in vitro, the hemolytic test, cellular uptake, pharmacokinetics, and the tissue distributions. The cellular uptake in vitro suggested that the uptake of Man-DLD-Chol-modified liposomes was significantly higher than that of unmodified liposomes in HepG2 cells. Pharmacokinetic parameters indicated that Man-DLD-Chol-GA-Lp was eliminated more rapidly than GA-Lp. In tissue distributions, the targeting efficiency (Te) of Man-DLD-Chol-GA-Lp on liver was 54.67%, relative targeting efficiency (RTe) was 3.39, relative uptake rate (Re) was 4.78, and peak concentration ratio (Ce) was 3.46. All these results supported the hypothesis that Man-DLD-Chol would be an efficient liposomal carrier, and demonstrated that Man-DLD-Chol-GA-Lp has potential as a drug delivery for liver-targeting therapy.Keywords:lipase-catalytic acylation;mannose-diester lauric diacid-cholesterol;glycyrrhetinic acid liposomes;mannose receptor;liver-targeting
研究领域:
药剂学
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生物化学
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DOI
PubMed
高影响因子
BIOMASS & BIOENERGY
·
2025
·
IF 5.8
Jingliang Xu,Shiwei Luo,Yaxian Qiao,Chunyu Li,Yuduo Zheng,Mohammad Asraful Alam,Shen Zhang
Mannose exhibits specific biological functions and can be acted as a precursor for many drugs. In this study, we used glucose as substrate for preparing mannose by thermos-chemical synthesis. Carbon nitride (C3N4) supported molybdenum and aluminum was prepared to selectively convert glucose to mannose and fructose in water. Results show that the yield and selectivity of mannose from glucose are 31.47 % and 85.00 %, respectively, at 130 °C for 5 min by C3N4 supported molybdenum (Mo-C3N4). The catalytic efficiency of Mo-C3N4 shown to be high. The yields of mannose and fructose are 18.72 % and 41.34 %, correspondingly and the Al-Mo-C3N4 exhibits a total selectivity of 91.91 % for mannose and fructose. C3N4 provided Brønsted bases (N-containing groups) facilitate isomerization of glucose to fructose. The Mo-C3N4 characterizations reveal that the 2D nanosheet structure embeds Mo species (MoO2, MoO3 and MoC2) at a size of 5–10 μm. The characterizations of Al-Mo-C3N4 proved the hierarchically porous structure and the homogeneous distributions of Al species (Al 2 O 3 ) and Mo species (Mo 4+ and Mo 6+ ) throughout the C3N4 skeletons. The work reveals a green and low-cost catalyst based on C3N4 support for highly selectivity catalyzing the conversion of glucose to mannose and fructose in aqueous.
研究领域:
化学
·
生物学
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DOI
高影响因子
LANGMUIR
·
2018
·
IF 3.789
Mei Liu,Jiao Li,Baoxin Li
Polyethylenimine (PEI) has antimicrobial activity against Gram-positive (Staphylococcus aureus, S. aureus) and Gram-negative (Escherichia coli, E. coli), bacteria but is highly cytotoxic, and the selective antimicrobial activity against S. aureus is obviously better than that against E. coli. To reduce the cytotoxicity and improve the antibacterial activity against E. coli, we modified PEI with d-mannose through nucleophilic addition between primary amine and aldehyde groups to get mannose-modified polyethylenimine copolymer particles (Man-PEI CPs). The use of mannose may provide good targeting ability toward E. coli pili. The antibacterial activity of Man-PEI CPs was investigated. Man-PEI CPs shows specific and very strong killing capability against E. coli at a concentration of 10 μg/mL, which is the highest antimicrobial efficiency compared to that of unmodified PEI (220 μg/mL). The antibacterial mechanism demonstrated that the enhancement in antibacterial activity is due to specific recognition of the mannose and destroying the cell wall of the bacteria by PEIs. Importantly, the Man-PEI CPs show less cytotoxicity and excellent biocompatibility. The results indicate that Man-PEI CPs have great potential as novel antimicrobial materials to prevent bacterial infections and provide specific applications for killing E. coli.
研究领域:
材料科学
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微生物学
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生物医学工程
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DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2025
·
IF 4.1
Luo Shiwei,Alam Mohammad Asraful,Gao Die,Li Yuexin,Zhao Lei,Xu MinQiang,Xu Jingliang,Zhang Shen
Mannose has demonstrated high value and versatility in various fields, leading to a growing interest in the catalytic epimerization of glucose to mannose using solid catalysts. Herein, zeolite-supported molybdenum (Mo) catalysts were prepared by impregnation-calcination (Mo/Z) and hydrothermal-calcination methods (Mo/Z-HT), respectively. HR-STEM characterization showed the diameter of Mo active sites (MoO 2 ) in Mo/Z and Mo/Z-HT are 57.83 ± 18.05 and 8.37 ± 1.62 nm, respectively. The hydrothermal-calcination process decreased the spatial scale of Mo active sites. The specific surface area of Mo/Z-HT (363.75 cm 3 /g) is higher than that of Mo/Z (200.46 cm 3 /g). The hydrothermal process promotes the formation of hierarchical structure of ZSM. Mo can be embedded in the hierarchical structure during the hydrothermal process, which improved the stability of catalysts significantly. Catalytic performance shown that Mo/Z-HT achieves a mannose yield of 32.81% with a selectivity of 94.20% in water at 130 °C for 30 min. Mo/Z achieved a mannose yield of 33.13% with a selectivity of 92.50% in ethanol-aqueous at 80 °C after 40 min. The recycling experiment showed that Mo/Z-HT exhibits high stability at the sixth cycle with a 30.76% yield and a selectivity of 94.55%. The different reaction temperatures may be attributed to ethyl glucoside was synthesized in ethanol media, which is more easily selectively epimerized in mild conditions. The protecting groups (-OC 2 H 5 ) at C1 position may decrease the activation energy of epimerization and realize kinetic control of site selectivity.
研究领域:
化学工程
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生物化学
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DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 19
·6654
·
IF 4.927
Wang Zhang,Xinjie Liu,Xun Sun,Rongchun Han,Nianjun Yu,Juan Liang,An Zhou
It is generally believed that freshDendrobium officinale(FDO) has more significant pharmacological activity than driedDendrobium officinale(DDO); however, the difference has not been clearly shown. Our study compared their antioxidant properties both in vitro and in vivo, and the molecular weight arrangement and monosaccharide composition of the freshDendrobium officinalepolysaccharides (FDOPs) and the driedDendrobium officinalepolysaccharides (DDOPs) were analyzed by HPLC-GPC and GC-MS. The results showed that the FDO and its polysaccharides had more significant effects on scavenging DPPH, ABTS, and hydroxyl radicals than the DDO. In addition, both the FDO and DDO significantly reduced lipid peroxidation levels and increased the SOD, T-AOC, CAT, and GSH levels in mice with acute liver damage caused by CCl4, while the FDO and its polysaccharides were more effective. Histopathological analysis further verified the protective effect of the Dendrobium polysaccharides on CCl4-induced liver injury. The determination of the polysaccharides revealed that the polysaccharide and mannose contents of the FDO were significantly higher than their dried counterparts, and the homogeneous arrangement of the polysaccharides in the FDO was degraded into three polysaccharide fragments of different molecular weights in the DDO. Overall, our data identified differences in the antioxidant activities of the FDO and DDO, as well as the reasons for these differences.Keywords:Dendrobium officinale;antioxidant activity;polysaccharide;mannose;monosaccharide composition
研究领域:
药理学
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植物学
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分析化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Yirui Wang,Shaowei Han,Ruofei Li,Baosong Cui,Xianjie Ma,Xinzhu Qi,Qi Hou,Mingbao Lin,Jinye Bai,Shuai Li
Two water-soluble polysaccharides , BSP-1 and BSP-2, were extracted and purified from the tuber of Bletilla striata . The molecular weights of BSP-1 and BSP-2 were 83.54 kDa and 12.60 kDa, respectively. The composition and proportion of BSP-1 and BSP-2 were mannose and glucose in molar ratios of 4.0:1.0 and 3.0:1.0 respectively. The two polysaccharides had a linear backbone, consisting mainly of repeating β‑1,4‑linked d ‑mannosyl residues and β‑1,4‑linked d ‑glucosyl residues. BSP-1 exhibited immunomodulatory effects by increasing the thymus and spleen indices of immunocompromised model mice.
研究领域:
植物化学
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免疫学
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DOI
PubMed
高影响因子
ANALYST
·
2023
·Vol. 148
·期 4
·912-918
·
IF 4.2
Xinai Zhang,Zhenzhong Wang,Xu Li,Wei Xiao,Xiaobo Zou,Qilin Huang,Lili Zhou
The development of effective methods for tracking cancer cells is of significant importance in the early diagnosis and treatment of tumor diseases. Compared with the developed techniques, the electrochemical assay has shown considerable potential for monitoring glycan expression on the cell surface using nondestructive means. However, the application expansion of the electrochemical strategy is strongly impeded owing to its dependence on electroactive species. In this study, a competitive electrochemical strategy was reported for monitoring cancer cells based on mannose (a typical glycan) as a clinical biomarker. Herein, functionalized carbon nanotubes were used to load the thiomannosyl dimer, and thionine-interlinking signal probes were designed for competitive recognition. After effective competition between cancer cells and the anchored mannose, a decreased current was obtained as the cell concentration increased. Under optimal conditions, the proposed biosensor exhibited attractive performance for cancer cell analysis with a detection limit as low as 20 cells per mL for QGY-7701 and 35 cells per mL for QGY-7703, facilitating great promise for the sensitive detection of cancer cells and thus showing potential applications in cancer diagnosis.
研究领域:
生物医学工程
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肿瘤学
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分析化学
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产品关联
DOI
PubMed
高影响因子
CELL RESEARCH
·
2023
·
IF 44.1
Ai Yuan-li,Wang Wei-jia,Liu Fan-jian,Fang Wei,Chen Hang-zi,Wu Liu-zheng,Hong Xuehui,Zhu Yuekun,Zhang Ci-xiong,Liu Long-yu,Hong Wen-bin,Zhou Bo,Chen Qi-tao,Wu Qiao
Pyroptosis is a type of regulated cell death executed by gasdermin family members. However, how gasdermin-mediated pyroptosis is negatively regulated remains unclear. Here, we demonstrate that mannose, a hexose, inhibits GSDME-mediated pyroptosis by activating AMP-activated protein kinase (AMPK). Mechanistically, mannose metabolism in the hexosamine biosynthetic pathway increases levels of the metabolite N -acetylglucosamine-6-phosphate (GlcNAc-6P), which binds AMPK to facilitate AMPK phosphorylation by LKB1. Activated AMPK then phosphorylates GSDME at Thr6, which leads to blockade of caspase-3-induced GSDME cleavage, thereby repressing pyroptosis. The regulatory role of AMPK-mediated GSDME phosphorylation was further confirmed in AMPK knockout and GSDME T6E or GSDME T6A knock-in mice. In mouse primary cancer models, mannose administration suppressed pyroptosis in small intestine and kidney to alleviate cisplatin- or oxaliplatin-induced tissue toxicity without impairing antitumor effects. The protective effect of mannose was also verified in a small group of patients with gastrointestinal cancer who received normal chemotherapy. Our study reveals a novel mechanism whereby mannose antagonizes GSDME-mediated pyroptosis through GlcNAc-6P-mediated activation of AMPK, and suggests the utility of mannose supplementation in alleviating chemotherapy-induced side effects in clinic applications.
研究领域:
癌症生物学
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细胞生物学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2024
·Vol. 26
·期 2
·1-13
·
IF 2.1
Ye Haiyang,Zhang Li,Qiu Xiaonan,Qiu Jianghong,Xu Wenqing,Xu Xiao,Yu Huijun,Zou Shengqiang,Du Fengyi,Zheng Guojun,Wu Yunchao
Photothermal therapy (PTT) can kill the tumor in situ, but fail to trigger strong antitumor immune response against its recurrence and metastasis. Herein, mannose-grafting Fe-doped carbon dots (M/Fe-CDs) were prepared via hydrothermal carbonization and chemical modification. The prepared M/Fe-CDs had characteristics of excellent optical qualities, good water solubility, and outstanding biocompatibility. Compared with pure CDs, the doping of Fe atom endowed the M/Fe-CDs with a high photothermal conversion efficiency (from 12.1 up to 30.1%). Under 808-nm NIR irradiation, the M/Fe-CDs could lead to local hyperthermia to kill tumor cells. Importantly, the M/Fe-CDs were capable of loading CpG-ODNs via electrostatic absorption and delivering them into the cytoplasm of dendritic cells (DCs). Finally, the CpG-ODNs-loading M/Fe-CDs could significantly increase the CD80/86 expression on the DCs surface. These results demonstrated that the M/Fe-CDs could be used as a versatile nanoplatform to combine the PTT and immune activation for tumor treatment.
研究领域:
生物医学工程
·
肿瘤免疫学
·
纳米医学
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DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2012
·
IF 3.456
Wantong Song,Chunsheng Xiao,Liguo Cui,Zhaohui Tang,Xiuli Zhuang,Xuesi Chen
Construction of high density glycosylated surfaces is important in the investigation of interactions between pathogens and surface carbohydrates. In this work, we provided a flexible method for glycosyl surface fabrication by combination of surface-initiated atom transfer radical polymerization (SI-ATRP) and copper-catalyzed azide-alkyne 1,3-dipolar cycloaddition (CuAAC) reaction. Through this strategy, we got a very high surface glycosyl density of about 4 nmol/cm 2 with the surface “click” efficiency of nearly 50%. Then the carbohydrate decorated surfaces were used to mimic cell surfaces and specific recognition of mannose with Escherichia coli was observed. We believe the methodology provided here can be used as a facile way for construction of a wide range of functional biosurfaces.
研究领域:
生物化学
·
材料科学
·
微生物学
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DOI
PubMed
高影响因子
BIOMATERIALS
·
2015
·
IF 8.557
Kaixuan Ren,Chaoliang He,Chunsheng Xiao,Gao Li,Xuesi Chen
Glycopolypeptides are an emerging class of bioinspired polymers that mimic naturally occurring glycopeptides or glycoproteins , and therefore are expected to exhibit great potential for biomedical applications . In this study, a glycopolypeptide was synthesized by conjugation of poly(γ-propargyl- l -glutamate) (PPLG) with azido-modified mannose and 3-(4-hydroxyphenyl) propanamide (HPPA), via click chemistry. Injectable hydrogels based on the glycopolypeptide were developed through enzymatic crosslinking reaction in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ). The physicochemical properties of the hydrogels, such as gelation time, storage modulus , swelling and degradation time , could be controlled by varying the concentrations of HRP and H 2 O 2 . The glycopolypetide copolymer as well as the extracts of the glycopolypetide hydrogels displayed good cytocompatibility in vitro . After subcutaneous injection into rats, the glycopolypeptide hydrogels were rapidly formed in situ , and exhibited acceptable biocompatibility accompanying the degradation of the hydrogels in vivo . The rabbit chondrocytes inside the glycopolypeptide hydrogels showed spherical morphology with high viability during the incubation period of 3 weeks in vitro , and exhibited a higher proliferation rate than within the hydrogel counterparts of PPLG grafted with 2-(2-(2-methoxyethoxy)ethoxy)ethane (MEO 3 ) and HPPA. Biochemical analysis demonstrated that the production of glycosaminoglycans (GAG) and type II collagen were significantly enhanced after incubation for 2 and 3 weeks in vitro . Moreover, the chondrocyte-containing glycopolypeptide hydrogels in subcutaneous model of nude mice maintained chondrocyte phenotype and produced the cartilaginous specific matrix. These results indicated that the biomimetic glycopolypeptide-based hydrogels hold potential as three-dimensional scaffolds for cartilage tissue engineering .
研究领域:
生物材料学
·
再生医学
·
组织工程
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DOI
PubMed
高影响因子
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