BMC PLANT BIOLOGY
·
2017
·Vol. 17
·期 1
·1-16
·
IF 3.964
Huang Yutao,Lin Cheng,He Fei,Li Zhan,Guan Yajing,Hu Qijuan,Hu Jin
Background The low seed vigor and poor field emergence are main factors that restricting the extension of sweet corn in China. Spermidine (Spd) plays an important role in plant growth and development, but little is known about the effect of Spd on sweet corn seed germination. Therefore the effect of exogenous Spd on seed germination and physiological and biochemical changes during seed imbibition of Xiantian No.5 were investigated in this study.Results Spd soaking treatment not only improved seed germination percentage but also significantly enhanced seed vigor which was indicated by higher germination index, vigor index, shoot heights and dry weights of shoot and root compared with the control; while exogenous CHA, the biosynthesis inhibitor of Spd, significantly inhibited seed germination and declined seed vigor. Spd application significantly increased endogenous Spd, gibberellins and ethylene contents and simultaneously reduced ABA concentration in embryos during seed imbibition. In addition, the effects of exogenous Spd on H2O2 and MDA productions were also analyzed. Enhanced H2O2 concentration was observed in Spd-treated seed embryo, while no significant difference of MDA level in seed embryo was observed between Spd treatment and control. However, the lower H2O2 and significantly higher MDA contents than control were detected in CHA-treated seed embryos.Conclusion sThe results suggested that Spd contributing to fast seed germination and high seed vigor of sweet corn might be closely related with the metabolism of hormones including gibberellins, ABA and ethylene, and with the increase of H2O2 in the radical produced partly from Spd oxidation. In addition, Spd might play an important role in cell membrane integrity maintaining.
研究领域:
植物生理学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2015
·Vol. 24
·期 3
·867-873
·
IF 0.653
Niu Liying,Li Dajing,Liu Chunquan,Liu Fuguo
Lipoxygenases (LOX) in milk-stage sweet corn and waxy corn were extracted using a phosphate buffer (pH 7.0) and enzyme activities were determined using linoleic acid as a substrate. Michaelis constant ( K m) values, decimal reduction times (D value), temperature sensitivity parameters (Z value), and activation energies ( E a) were calculated. Enzymes from both corn types followed first-order inactivation kinetics within 0–25 min and 50–70°C. However, enzymes exhibited different pH profiles and affinities toward linoleic acid. Km values (4.34 and 1.40 mM for sweet corn and waxy corn, respectively), heat stability values, and Ea values (116.81 and 246.82 kJ/mol) were different. Waxy corn LOX was more heat stable below 65–C with a higher D value, but was more temperature sensitive with a lower Z value. The different characteristics suggested the presence of different isoenzymes and necessitated the use of different parameters for blanching.
研究领域:
食品科学
·
植物生理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PLANT BIOLOGY
·
2021
·Vol. 64
·期 6
·487-499
·
IF 2.434
Cai Shuyu,Wang Guofu,Xu Hua,Liu Jianxin,Luo Jie,Shen Yi
Chilling stress is a major abiotic constraint that affects sweet corn seedling establishment. Spermidine (Spd) is known to participate in the cold stress response in plants, nonetheless, the molecular mechanisms are largely unknown. The present study reported that exogenous Spd not only stimulated seedling growth but also enhanced photosynthetic attributes under chilling stress. Further analysis revealed that Spd application resulted in depressed abscisic acid (ABA) content and affected the expression of ABA-responsive genes. The augmented antioxidant enzymes activities, coupled with declined reactive oxygen species (ROS) content, were observed in response to Spd treatment under chilling stress. Moreover, Spd up-regulated the transcription levels of some Ca 2+ transport-related genes. We propose that Spd contributes to cold tolerance in sweet corn seedlings via the regulation of genes involved in the ROS, ABA pathways and Ca 2+ transport.
研究领域:
植物生理学
·
逆境生物学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
ACS Food Science & Technology
·
2022
·
IF 0
Wenxia Zhang,Baojie Zhu,Holly Childs,Monica Whent,Liangli Yu,Pamela R. Pehrsson,Jing Zhao,Xianli Wu,Shaoping Li
Sweet corn is an important source of carbohydrates in the human diet. In this study, the effects of the two most commonly used cooking methods (boiling and steaming) on the contents of individual sugars, sugar alcohols, oligosaccharides, total starch, resistant starch (RS), phytoglycogen, water-soluble carbohydrates (WSC), and dietary fibers (DF) were investigated using yellow and bicolor sweet corn samples. Four simple sugars, sucrose, glucose, fructose, and maltose, were identified. Boiling and steaming did not alter the concentrations of sucrose but resulted in the decrease of other three minor sugars. Sorbitol and mannitol were identified as the primary sugar alcohols. Sorbitol decreased in cooked samples, while mannitol remained unchanged regardless of the cooking method. Oligosaccharides and phytoglycogen were not detected in either variety. Boiling and steaming did not alter the content of WSC, total starch, or higher-molecular-weight DF but led to a significant reduction of RS and total DF. This study provided understanding on how cooking may affect the health benefits and quality of sweet corn.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
DRYING TECHNOLOGY
·
2023
·
IF 3.3
Mengru Li,Bo Wang,Yuchuan Wang,Jiguang Liu,Min Zhang
The homogeneity of dried products produced via pilot-scale pulse-spouted bed microwave (915 MHz) freeze-drying (pilot-scale PSBMFD) was investigated using sweet corn, potato, banana and hawthorn as experimental materials. The quality and energy cost of the dried products were also investigated. Regarding the moisture content, color difference and shrinkage ratio, pilot-scale PSBMFD achieved high drying uniformity (⩾90%, d.b.). Compared with the conventional freeze-drying (CFD) method used in industries, pilot-scale PSBMFD effectively increased the nutrient retention (d.b.) in dried samples. It increased the retention of flavonoids in sweet corn, potato cube, banana slice and hawthorn by approximately 11.68%, 15.82%, 5.76%, and 15.94% and the total phenolic content by 11.85%, 23.58%, 28.34%, and 24.17%, respectively. Further, pilot-scale PSBMFD resulted in a larger honeycomb porous structure, greater hardness and brittleness as well as shrinkage and lower apparent density than CFD. Regarding the energy cost, pilot-scale PSBMFD can save more energy than CFD (⩾39%, d.b.). This pilot-scale experimental study provides a theoretical basis and technical support for the commercial application of the PSBMFD technology.
研究领域:
食品科学与工程
·
农产品加工
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Xin Wang,Jingyang Wang,Yu Luo,Weiye Xiu,Shiyou Yu,Mengyuan Yang,Kechi Zhou,Yongqiang Ma
Fluorescein isothiocyanate (FITC) was used for the fluorescent labeling of sweet corn cob polysaccharide (SCP), followed by identification using fluorescence spectroscopy, UV–Visible spectroscopy, infrared spectroscopy, and NMR analysis. The results confirmed the successful attachment of FITC to SCP with a substitution degree of 1.28%. This established fluorescent sweet corn cob polysaccharide (FSCP) was then subjected to an in vivo quantitative analysis, in which mice were administered with FSCP, fluorescent sweet corn cob polysaccharide nanoemulsion (FSCP-NE), and fluorescent sweet corn cob polysaccharide nano emulsion microcapsule (FSCP-NE-ME) via gavage followed by investigating the in vivo plasma pharmacokinetics and tissue distribution. The results revealed that FSCP-NE could improve the utilization efficiency of SCP, and the FSCP-NE microcapsules exhibited good controlled-release performance, which significantly improved the utilization efficiency of SCP. The absorption and metabolism of SCP in vivo were explored next, which provided the reference for future research and development on SCP for application in the fields of nutraceuticals and pharmaceuticals.
研究领域:
药理学
·
纳米技术
·
生物技术
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2017
·Vol. 18
·期 6
·1246
·
IF 3.226
Nan Xiang,Xinbo Guo,Fengyuan Liu,Quan Li,Jianguang Hu,Charles Stephen Brennan
Sweet corn is one of the most widely planted crops in China. Sprouting of grains is a new processes to increase the nutritional value of grain products. The present study explores the effects of light on the nutritional quality of sweet corn sprouts. Gene expression of phenolic biosynthesis, phytochemical profiles and antioxidant activity were studied. Two treatments (light and dark) were selected and the morphological structure of sweet corn sprouts, as well as their biochemical composition were investigated to determine the effects of light on the regulation of genes responsible for nutritional compounds. Transcription analyses for three key-encoding genes in the biosynthesis of the precursors of phenolic were studied. Results revealed a negative regulation in the expression ofZmPALwith total phenolic content (TPC) in the light group. TPC and total flavonoid content (TFC) increased during germination and this was correlated with an increase in antioxidant activity (r= 0.95 and 1.0). The findings illustrate that the nutritional value of sweet corn for the consumer can be improved through germination to the euphylla stage.Keywords:germination;sweet corn;phenolic biosynthesis;antioxidant activity
研究领域:
植物科学
·
营养学
·
分子生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）