JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2018
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IF 2.196
Behnaz Asadzadeh,Jialun Zhong,Weidong Yan
Solid–liquid equilibrium data of rebaudioside A (a natural sweetener) in nine pure solvents (methanol, ethanol, 1-propanol, 2-propanol, acetonitrile, 2-methoxyethanol, 2-ethoxyethanol, 2-buthoxyethanol, and ethylene glycol) and two binary mixed solvents (methanol + ethanol and 2-methoxyethanol + ethanol) were been measured by high performance liquid chromatography at temperatures ranging from (288.15 to 328.15) K and atmospheric pressure. From the experimental data, it was found that the solubility increased with the rising temperature in all studied solvents. The obtained values for the solubility of rebaudioside A in pure and mixed binary solvents were correlated with modified Apelblat and nonrandom two-liquid models. The results showed that these models can accurately correlate the solubility data obtained in this work. Furthermore, the crystal habits of rebaudioside A in selected solvents were studied.
研究领域:
食品科学
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物理化学
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DOI
高影响因子
Antioxidants
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2021
·Vol. 10
·期 2
·262
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IF 6.313
Pan Li,Zehua Wang,Sin Man Lam,Guanghou Shui
Non-nutritive sweeteners are widely used in food and medicines to reduce energy content without compromising flavor. Herein, we report that Rebaudioside A (Reb A), a natural, non-nutritive sweetener, can extend both the lifespan and healthspan ofC. elegans. The beneficial effects of Reb A were principally mediated via reducing the level of cellular reactive oxygen species (ROS) in response to oxidative stress and attenuating neutral lipid accumulation with aging. Transcriptomics analysis presented maximum differential expression of genes along the target of rapamycin (TOR) signaling pathway, which was further confirmed by quantitative real-time PCR (qPCR); while lipidomics uncovered concomitant reductions in the levels of phosphatidic acids (PAs), phosphatidylinositols (PIs) and lysophosphatidylcholines (LPCs) in worms treated with Reb A. Our results suggest that Reb A attenuates aging by acting as effective cellular antioxidants and also in lowering the ectopic accumulation of neutral lipids.Keywords:C. elegans;non-nutritive sweetener;rebaudioside A;aging;oxidative stress resistance;TOR;lipidomics
研究领域:
衰老
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分子生物学
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遗传学
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生物化学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2024
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IF 3.7
Zhenguang Liu,Zexiang Ding,Yifeng Cao,Baojian Liu,Qiwei Yang,Zhiguo Zhang,Qilong Ren,Zongbi Bao
The equilibrium solubility of Rebaudioside A (Reb A) Form II in binary mixtures of methanol/ethanol and ethyl acetate was quantitatively determined within the temperature range of 283.15 to 328.15 K at ambient pressure. The experimental findings indicate a positive correlation between the solubility of Reb A (Form II) and both the temperature and the methanol/ethanol content in the solvent system. To describe the solubility data, six distinct models were employed: the modified Apelblat equation, the λh model, the CNIBS/R-K model, the van't Hoff-Jouyban-Acree (VJA) model, the Apelblat-Jouyban-Acree (AJA) model, and the Non-Random Two-Liquid (NRTL) model. The CNIBS/R-K model exhibited the most precise fit for solubility in methanol+ethyl acetate mixtures, reflected by an Average Relative Deviation (ARD) of 0.0011 and a Root Mean Square Deviation (RMSD) of 0.0000012. Conversely, for ethanol+ethyl acetate mixtures, the modified Apelblat equation provided a superior correlation (ARD=0.0014, RMSD=0.0000004). Furthermore, thermodynamic parameters associated with the dissolution of Reb A (Form II), including enthalpy, entropy, and the Gibbs energy change, were inferred from the data. The findings underscore that the dissolution process is predominantly endothermic across the solvent systems examined. Notably, the entropy changes appear to have a significant influence on the Gibbs free energy associated with the dissolution of Reb A (Form II), suggesting that entropic factors may play a pivotal role in the studied systems.
研究领域:
化学工程
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物理化学
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热力学
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DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
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2018
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IF 3.04
Yuxin Wang,Linyao Li,Yawen Wang,Xiaokang Zhu,Mingdong Jiang,Erqun Song,Yang Song
A large population of drug candidates have failed “from bench to bed” due to unwanted toxicities. We intend to develop an alternative approach for drug discovery, that is, to seek candidates from “safe” compounds. Rebaudioside A (Reb-A) is an approved commercial sweetener from Stevia rebaudiana Bertoni. We found that Reb-A protects against carbon tetrachloride (CCl 4 )-induced oxidative injury in human liver hepatocellular carcinoma (HepG2) cells. Reb-A showed antioxidant activity on reducing cellular reactive oxygen species and malondialdehyde levels while increasing glutathione levels and superoxide dismutase and catalase activities. Reb-A treatment induced nuclear factor erythroid-derived 2-like 2 (Nrf2) activation and antioxidant response element activity, as well as the expression of heme oxygenase-1 (HO-1) and NAD(P)H quinone oxidoreductase 1 (NQO1). Further mechanistic studies indicated that c-Jun N-terminal kinase (JNK), extracellular signal-regulated protein kinase (ERK), mitogen-active protein kinase (MAPK) and protein kinase C epsilon (PKCε) signaling was upregulated. Thus, the present in vitro study conclusively demonstrated that Reb-A is an activator of Nrf2 and is a potential candidate hepatoprotective agent. More importantly, the present study illustrated that seeking drug candidates from “safe” compounds is a promising strategy.
研究领域:
药理学
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毒理学
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细胞生物学
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分子生物学
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药物发现
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）