JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
·
2015
·
IF 2.128
Abubakr Musa,Ming Miao,Mohammed A.A. Gasmalla,Tao Zhang,Ahmed Eibaid,Waleed Aboshora,Bo Jiang
In this study, effect of shaking velocity on mono-glycosyl-stevioside production by Leuconostoc citreum SK24.002 alternansucrase acceptor reaction was investigated, with four different level of shaking velocity (75, 100, 125 and 150 rpm) up to 24 h at 25 °C, using sucrose as donor and stevioside as acceptor. The results revealed that mono-glycosyl-stevioside yield significantly increased with increase in the reaction shaking velocity and reached maximum yield of 3.78 ± .02 mg/mL at 150 rpm shaking velocity after 6 h of reaction. And an increase in the reaction shaking velocity up to 150 rpm reduced the final reaction time and increased the productivity of mono-glycosyl-stevioside product such that the high quantity of mono-glycosyl-stevioside product was produced in only 6 h rather than 24 h. The mono-glycosyl-stevioside product was completely separated using macroporous resin AB-8 flowed by semi-preparative HPLC. Macroporous resin AB-8 showed high selectivity and capacity toward mono-glycosyl-stevioside. The structure of mono-glycosyl-stevioside was characterized, as 13-{[α- d -glucopyranosyl-(1→6)-β- d -glucopyranosyl-(1→2)-β- d -glucopyranosyl]oxy}kaur-16-en-19-oic acid β- d -glucopyranosyl eater, a corroding to extensive 1D and 2D NMR (1H and 13C, COSY, HSQC, HMBC) and mass spectral data.
研究领域:
生物化学
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食品科学
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产品关联
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2019
·Vol. 13
·期 2
·1159-1165
·
IF 1.415
Musa Abubakr,Jiang Bo,Ma Haile,Gasmalla Mohammed A. A.,Abdalhai Mandour H.,Al-alfarga Ammar
In this work, alternansucrase enzyme from Leuconostoc citreum SK24.002 was used to produce di-glycosyl-stevioside through acceptor reaction. This was detected by high-performance liquid chromatography (HPLC) and was isolated using AB-8 macroporous resin and semi-preparative HPLC. With the help of mass spectroscopy, 1D NMR (H 1 and C 13 ) and 2D NMR (COSY, HMQC, HMBC) the di-glycosyl-stevioside structure was identified to be 13-{[α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-β-D glucopyranosyl-(1→2)-β-D-glucopyranosyl]oxy} kaur-16-en-19-oic acid β-D glucopyranosyl eater. In order to increase the modified stevioside (di-glycosyl-stevioside) product and reduce production time, the impact of velocity of shaking on the di-glycosyl-stevioside production was also evaluated, using 75–150 rpm for 24 h at 25 °C. The results showed that the di-glycosyl-stevioside production increased significantly (p < 0.05) from 0.35 ± 0.0.01 mg/mL for 75 rpm to 1.0667 ± 0.0152 mg/mL for 150 rpm at 6 h during the glycosylation reaction.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 2
·216
·
IF 4.2
Samra Basharat,Lixin Zhai,Fuyao Jiang,Tanzila Asjad,Adil Khan,Xiangru Liao
The purpose of this research is to identify and characterize lactic acid bacteria (LAB) species in bee bread produced by honey bees (Apis Cerana) in the east mountain area of Suzhou, China. We isolated three strains,Apilactobacillus kunkeei(S1),Lactiplantibacillus plantarum(S2), andLacticaseibacillus pentosus(S3), with S2 producing the highest amount of lactic acid. Phylogenetic analysis indicated that these isolates, along with the type strain, formed a distinct sub-cluster within the LAB group. The strains exhibited non-hemolytic activity, lacked functional virulence factors, demonstrated high acid and bile tolerance, strong adhesion to intestinal cells, and antimicrobial activity against pathogens, collectively indicating their safety and high probiotic potential for therapeutic applications. Our studies demonstrated that S2 and S3 grew well in the presence of stevia leaf powder and steviosides, while S1 showed reduced growth and inhibitory effects. Importantly, the stevia-fermented strains exhibited strong probiotic potential along with significant antidiabetic, antioxidant, and antifungal properties in vitro. These findings highlight their potential applications in the food, feed, and pharmaceutical industries. Future research should focus on in vivo experiments to validate these results and evaluate compatibility among the strains before their application in functional foods.
研究领域:
微生物学
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食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 16
·3416
·
IF 4.6
Zidan Liu,Linyu Luo,Zhiqiang Ding,Weihao Long,Tolbert Osire,Qiong Li,Qianfeng Chen,Mengfei Long
Stevioside, a natural high-intensity sweetener, is widely employed across the food, pharmaceutical, and daily chemical industries due to its intense sweetness and health benefits. However, traditional extraction and purification processes for steviol glycosides fromStevia rebaudianaare plagued by low efficiency, high energy consumption, substantial environmental impact, and inconsistent product quality. This study systematically optimized the extraction, decolorization, decontamination, and desalting processes to overcome these challenges. The extraction method was refined using 20% ethanol as the solvent, an optimal temperature of 50 °C, and a 1:10 material-to-liquid ratio, increasing the steviol glycoside yield from 32.0% to 49.1%. Decolorization employing a combination of resins D940 and T5 achieved decolorization rates of 89–92% with minimized steviol glycoside loss, surpassing the non-selective adsorption limitations of activated carbon. For decontamination, calcium hydroxide (Ca(OH)2) outperformed diatomaceous earth, attaining a 98% protein removal rate while maintaining steviol glycoside loss below 20%. The desalting resin LXP-016 demonstrated superior performance at 40 °C, enhancing the ability of ionic impurity removal. These optimizations collectively improve the efficiency, sustainability, and quality of steviol glycoside production, offering a promising framework for industrial-scale applications.
研究领域:
植物生物技术
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食品科学
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药学
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化学工程
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Zhuqing Dai,Jiangfeng Song,Ye Chen,Lei Feng,Yayuan Xu,Dajing Li,Caie Wu,Zhongyuan Zhang,Jun Liu
This study aims to develop novel lutein nanoparticles encapsulized by stevioside (LUT-STE, 165 ± 2 nm average particles size) and systematically evaluate its bioavailability. Multiple spectroscopy and NMR analyses showed lutein and stevioside could interact through hydrogen bonds, C H π interaction and van der Waals forces. Molecular docking simulation showed lutein was well distributed in the hydrophobic cavity of stevioside. Analyzed by Caco-2 cellular models, the transported amount of LUT-STE was 2.39 times that of lutein in 120 min with a P app (B → A)/ P app (A → B) value of 0.63 ± 0.04. Nystatin and dynasore significantly reduced the cellular uptake of LUT-STE by 41.3% and 57.7%, respectively. Compared with free lutein, LUT-STE increased the C max in mice plasma by 5.01-fold and promoted the accumulation in multiple organs. LUT-STE promoted the protein expressions of CD36, NPC1L1 and PPARγ in both cell and animal models. In conclusion, stevioside entrapment significantly promote the bioavailability of lutein through multiple transmembrane pathways.
研究领域:
药学
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营养学
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分子生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
Canadian Journal of Chemistry
·
2020
·Vol. 98
·期 11
·
IF 1.171
Guanyu Wang,Qiaoge Fu,Ruili Guo,Zhong Wei
The boronic acid functionalization metal-organic frameworks (MOFs), as unique boronate affinity adsorbents, have desired specific molecular affinity for the separation and enrichment of cis-diol-compounds. Herein, the boronic acid functionalized Zn-based MOF adsorbent (MOF-BA) was synthesized through a simple one-step microwave method and used for the recognition and isolation of steviol glycosides (SGs). This MOF-BA exhibits the same spherical structure and isostructure with the parent framework composed only of the primitive ligand as verified by SEM and XRD characterization. It was confirmed that changing the ratio of ligands could achieve the adjustability of the boron content in the framework. At the same time, the MOF-BA-1.0 showed a suitable pore size (4.69 nm), and the presence of boric acid functional groups showed favorable selectivity for stevioside (STV). The static adsorption results showed that adsorption performances of rebaudioside A (RA) and STV from crude sugar solution (5.0 mg mL−1, pH 8) on MOF-BA-1.0 were investigated at 303 K for 15 h. The adsorption capacities for STV and RA were 42.93 mg g−1 and 22.96 mg g−1, respectively, and the adsorption selectivity (αSTV/RA) reached 4.35. The adsorption isotherm and kinetic data of MOF-BA-1.0 for RA and STV obeyed the Langmuir isotherm model and pseudo second order kinetic model, respectively. The study demonstrated that MOF-BA-1.0 adsorbent could be used as a potential adsorbent to purify the active ingredients of stevia and obtain a high concentration of RA products.
研究领域:
化学
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材料科学
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药学
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分析化学
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2017
·Vol. 409
·期 28
·6537-6547
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IF 3.431
Yang Yang,Zhong Qisheng,Mo Canlong,Zhang Hao,Zhou Ting,Tan Wen
Accurate and reliable quantification of endogenous lipid mediators in complex biological samples is a daunting challenge. In this study, a robust and direct endogenous quantitative method using background subtracting calibration curves by liquid chromatography-tandem mass spectrometry was first developed for the determination of endogenous lipid mediators in ischemic stroke rats. Absolute quantification without surrogate matrix could be achieved by using background subtracting calibration curves, which were corrected and verified from standard curves constructed on original matrix. The recoveries of this method were in the range of 50.3–98.3%, the precision with the relative standard deviation was less than 13.8%, and the accuracy with the relative error was within ± 15.0%. In addition, background subtracting calibration curves were further verified by validation factors ranging from 90.3 to 110.9%. This validated method has been successfully applied to the analysis of seven endogenous inflammation-related lipid mediators in the brain tissues of ischemic stroke rats. The results indicated that prostaglandins as inflammatory factors and some lipid mediators with neuroprotective effects increased apparently ( p < 0.05) in the stroke groups compared with the normal rats. Besides, the two drugs (isosteviol sodium and edaravone) could significantly reduce ( p < 0.05) the levels of prostaglandin E 2 and prostaglandin F 2α of stroke rats to inhibit inflammation. Based on the results, it is strongly believed that this approach can be readily generalized as a new reference for the quantification of endogenous compounds in the complex biological samples.
The analysis procedure of determining endogenous inflammation-related lipid mediators using BSCC by LC-MS/MS
研究领域:
分析化学
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神经科学
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药理学
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产品关联
DOI
PubMed
高影响因子
Science Advances
·
2023
·Vol. 9
·期 18
·
IF 13.6
Yunxiang Bai,Beibei Liu,Jiachen Li,Minghui Li,Zheng Yao,Liangliang Dong,Dewei Rao,Peng Zhang,Xingzhong Cao,Luis Francisco Villalobos,Chunfang Zhang,Quan-Fu An,Menachem Elimelech
The successful implementation of thin-film composite membranes (TFCM) for challenging solute-solute separations in the pharmaceutical industry requires a fine control over the microstructure (size, distribution, and connectivity of the free-volume elements) and thickness of the selective layer. For example, desalinating antibiotic streams requires highly interconnected free-volume elements of the right size to block antibiotics but allow the passage of salt ions and water. Here, we introduce stevioside, a plant-derived contorted glycoside, as a promising aqueous phase monomer for optimizing the microstructure of TFCM made via interfacial polymerization. The low diffusion rate and moderate reactivity of stevioside, together with its nonplanar and distorted conformation, produced thin selective layers with an ideal microporosity for antibiotic desalination. For example, an optimized 18-nm membrane exhibited an unprecedented combination of high water permeance (81.2 liter m−2 hour−1 bar−1), antibiotic desalination efficiency (NaCl/tetracycline separation factor of 11.4), antifouling performance, and chlorine resistance.
研究领域:
材料科学
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制药工程
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环境科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Shuwei Luo,Yan Zhang,Jiangfeng Song,Ying Li,Caie Wu,Chenchen Zhang
This study investigated the solubilizing capacity of glycosylated stevioside/hydroxypropyl-methylcellulose (stevia-G-HPMC) complexes with varying mass ratios on lutein . The impact on the steady-state flux and permeability coefficient of intracellular lutein was also explored through the construction of a Caco-2 cellular transport model. The results indicated that the equilibrium solubility of lutein linearly increased with an increase in stevia-G amount. The stability constants of the ternary system surpassed those of the binary system. Molecular dynamics simulation revealed a tight and stable structure in lutein supersaturated complexes. Meanwhile, lutein-stevia-G-HPMC complexes demonstrated superior cumulative penetrations, with the peak P app (AP → BL) value being (3.24 ± 0.89) × 10 −5 cm·s −1 . There was a slight decrease in P app (BL → AP), which improved the forward transport of lutein. Highly soluble lutein in aqueous environments saturated the extracellular transport proteins on the AP side of cell membranes, thereby maintaining the high permeability transport. Notably, the permeability trend of lutein in Caco-2 cells negatively correlated with the equilibrium solubility and matched the single exponential growth model. When the mass ratio of lutein, stevia-G and HPMC was 1:21:5, the solubility-permeability trade-off of lutein was effectively maintained.
研究领域:
药学
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药物递送系统
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生物药剂学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 3
·2027-2035
·
IF 3.9
Zhibin Dong,Fengjun Ma,Xiaocen Wei,Linlin Zhang,Yongling Ding,Lei Shi,Chen Chen,Yuxia Ma,Yuning Ma
Herbal hydrogels as a new class of sustainable functional materials have attracted extensive attention. However, the development of herbal hydrogels is significantly hindered due to their poor hydrogel performances and the lack of universal preparation methods. In this study, four herbal hydrogels composed of phytochemical polyphenols and stevioside compounds are prepared through a facile heating-cooling process, where multiple hydrogen bonding interactions between two monomers provide the main driving force for gelation. These herbal hydrogels exhibit thermo-sensitivity and good reversibility (25–90 °C), robust adhesion behaviours on hydrophilic and hydrophobic surfaces (maximum adhesion strength of 591.7 kPa), and outstanding antibacterial properties (100% bacteriostatic ratio). Profiting from these intriguing characteristics, they are demonstrated to show great potential as natural antibacterial coatings by depositing thin hydrogel layers onto diverse substrates. More importantly, the hydrogel coatings could be easily recycled by thermal regelation and reused at least 5 times. This work proposes a simple and universal strategy for preparing functional hydrogels based on binary herbal small molecules, which also sheds light on the development of reusable hydrogel coatings.
研究领域:
材料科学
·
药学
·
生物技术
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Yutong Jiang,Xin Wen,Jiahui Guo,Hongyu Li,Zhanmei Jiang,Yun Chen
This study investigated how tea saponin (Ts), gypenoside (Gyp), mogroside V(Mog), glycyrrhizic acid (GA) and stevioside (STE) affected the heat-induced aggregates of α-lactalbumin (Ala), focusing on inhibition mechanisms of disulfide cross-linking and their impact on structural and physicochemical properties. SDS-PAGE and size exclusion chromatography (SEC) confirmed that saponins probably bound Ala monomers via hydrophobic forces and hydrogen bonds, inhibiting the formation of disulfide bonds in heat-induced Ala (H-Ala) and effectively decreasing polymer generation, with the inhibitory effect in the order of Gyp, GA, Ts, STE, Mog. LC-MS/MS analysis revealed that Ala (6), Ala (91), Ala (111) and Ala (120) were the most active sites for the disulfide crosslinking reaction in H-Ala. Specifically, the number of intramolecular disulfide cross-linked peptides of heat-induced Ala-Mog, Ala-STE, Ala-GA, Ala-Ts and Ala-Gyp complexes significantly decreased by 5, 6, 7, 8 and 10, respectively, compared with H-Ala. Notably, the heat-induced Ala-Gyp complex exhibited the highest absolute zeta potential, smallest particle size, lowest hydrophobicity, which correlated with its superior inhibition of aggregation. The study highlighted the potential of saponins as natural additives for the stabilization of heat-sensitive proteins during the thermal processing, offering new insights for food industry applications.
研究领域:
食品科学
·
分子生物学
·
生物物理化学
·
蛋白质工程
·
天然产物化学
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产品关联
DOI
高影响因子
Food Analytical Methods
·
2015
·Vol. 8
·期 10
·2588-2594
·
IF 1.956
Jiang Ting-Fu,Chong Lei,Yue Mei-E,Wang Yuan-Hong,Lv Zhi-Hua
In this study, the separation and determination of carbohydrates in food samples were developed by capillary electrophoresis using dynamically coating the capillary with indirect UV detection. The polycationic polymers of hexadimethrine bromide (HDB) were used as electroosmotic flow (EOF) modifier to reverse the EOF. A highly alkaline pH condition was used in order to charge every carbohydrate negatively. Several important parameters influencing buffer concentration, pH, and HDB concentration were optimized. The optimal background electrolyte composed of 20 mmol L −1 sorbic acid, 0.005 % ( w / v ) HDB, and 40 mmol L −1 NaOH at pH 12.2. Under optimal conditions, 32 carbohydrates including mono-, di-, oligosaccharides, and sugar alcohols were separated in less than 12 min. The proposed method provided a good linearity, low limits of detection, and good repeatability of the carbohydrates. This method was then utilized to analyze carbohydrates in food samples. The results indicated that the developed method is an excellent alternative for the carbohydrate routine analysis in the food field.
研究领域:
分析化学
·
食品科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）