JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
·
2015
·
IF 2.128
Abubakr Musa,Ming Miao,Mohammed A.A. Gasmalla,Tao Zhang,Ahmed Eibaid,Waleed Aboshora,Bo Jiang
In this study, effect of shaking velocity on mono-glycosyl-stevioside production by Leuconostoc citreum SK24.002 alternansucrase acceptor reaction was investigated, with four different level of shaking velocity (75, 100, 125 and 150 rpm) up to 24 h at 25 °C, using sucrose as donor and stevioside as acceptor. The results revealed that mono-glycosyl-stevioside yield significantly increased with increase in the reaction shaking velocity and reached maximum yield of 3.78 ± .02 mg/mL at 150 rpm shaking velocity after 6 h of reaction. And an increase in the reaction shaking velocity up to 150 rpm reduced the final reaction time and increased the productivity of mono-glycosyl-stevioside product such that the high quantity of mono-glycosyl-stevioside product was produced in only 6 h rather than 24 h. The mono-glycosyl-stevioside product was completely separated using macroporous resin AB-8 flowed by semi-preparative HPLC. Macroporous resin AB-8 showed high selectivity and capacity toward mono-glycosyl-stevioside. The structure of mono-glycosyl-stevioside was characterized, as 13-{[α- d -glucopyranosyl-(1→6)-β- d -glucopyranosyl-(1→2)-β- d -glucopyranosyl]oxy}kaur-16-en-19-oic acid β- d -glucopyranosyl eater, a corroding to extensive 1D and 2D NMR (1H and 13C, COSY, HSQC, HMBC) and mass spectral data.
研究领域:
生物化学
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食品科学
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产品关联
DOI
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 2
·216
·
IF 4.2
Samra Basharat,Lixin Zhai,Fuyao Jiang,Tanzila Asjad,Adil Khan,Xiangru Liao
The purpose of this research is to identify and characterize lactic acid bacteria (LAB) species in bee bread produced by honey bees (Apis Cerana) in the east mountain area of Suzhou, China. We isolated three strains,Apilactobacillus kunkeei(S1),Lactiplantibacillus plantarum(S2), andLacticaseibacillus pentosus(S3), with S2 producing the highest amount of lactic acid. Phylogenetic analysis indicated that these isolates, along with the type strain, formed a distinct sub-cluster within the LAB group. The strains exhibited non-hemolytic activity, lacked functional virulence factors, demonstrated high acid and bile tolerance, strong adhesion to intestinal cells, and antimicrobial activity against pathogens, collectively indicating their safety and high probiotic potential for therapeutic applications. Our studies demonstrated that S2 and S3 grew well in the presence of stevia leaf powder and steviosides, while S1 showed reduced growth and inhibitory effects. Importantly, the stevia-fermented strains exhibited strong probiotic potential along with significant antidiabetic, antioxidant, and antifungal properties in vitro. These findings highlight their potential applications in the food, feed, and pharmaceutical industries. Future research should focus on in vivo experiments to validate these results and evaluate compatibility among the strains before their application in functional foods.
研究领域:
微生物学
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食品科学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Canadian Journal of Chemistry
·
2020
·Vol. 98
·期 11
·
IF 1.171
Guanyu Wang,Qiaoge Fu,Ruili Guo,Zhong Wei
The boronic acid functionalization metal-organic frameworks (MOFs), as unique boronate affinity adsorbents, have desired specific molecular affinity for the separation and enrichment of cis-diol-compounds. Herein, the boronic acid functionalized Zn-based MOF adsorbent (MOF-BA) was synthesized through a simple one-step microwave method and used for the recognition and isolation of steviol glycosides (SGs). This MOF-BA exhibits the same spherical structure and isostructure with the parent framework composed only of the primitive ligand as verified by SEM and XRD characterization. It was confirmed that changing the ratio of ligands could achieve the adjustability of the boron content in the framework. At the same time, the MOF-BA-1.0 showed a suitable pore size (4.69 nm), and the presence of boric acid functional groups showed favorable selectivity for stevioside (STV). The static adsorption results showed that adsorption performances of rebaudioside A (RA) and STV from crude sugar solution (5.0 mg mL−1, pH 8) on MOF-BA-1.0 were investigated at 303 K for 15 h. The adsorption capacities for STV and RA were 42.93 mg g−1 and 22.96 mg g−1, respectively, and the adsorption selectivity (αSTV/RA) reached 4.35. The adsorption isotherm and kinetic data of MOF-BA-1.0 for RA and STV obeyed the Langmuir isotherm model and pseudo second order kinetic model, respectively. The study demonstrated that MOF-BA-1.0 adsorbent could be used as a potential adsorbent to purify the active ingredients of stevia and obtain a high concentration of RA products.
研究领域:
化学
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材料科学
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药学
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分析化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
·
2018
·
IF 2.317
Jun-Ming Chen,Yong-Mei Xia,Yan-Dong Zhang,Tong-Tong Zhang,Qing-Rui Peng,Yun Fang
Steviol glycosides, a natural sweetener, may perform bioactivities via steviol, their main metabolite in human digestion. The metabolising kinetics, i.e. glucuronidation kinetics and interaction between steviol glycosides or their metabolites and metabolising enzyme, are important for understanding the bioactivity and cytotoxicity. The present study investigated kinetics of steviol glucuronidation in human liver microsome and a recombinant human UDP-glucuronosyltransferases isomer, UGT2B7, along with molecular docking to analyse interaction between UGT2B7 and steviol or glucose. The active pocket of UGT2B7 is consisted of Arg352, Leu347, Lys343, Phe339, Tyr354, Lys355 and Leu353. The influence of stevioside, rebaudioside A, glucose and some chemotherapy reagents on the glucuronidation was also studied. The predicted hepatic clearence suggested that steviol could be classified as high-clearence drug. The steviol glycosides did not affect the glucuronidation of steviol notably.
研究领域:
药理学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2018
·
IF 2.196
Behnaz Asadzadeh,Jialun Zhong,Weidong Yan
Solid–liquid equilibrium data of rebaudioside A (a natural sweetener) in nine pure solvents (methanol, ethanol, 1-propanol, 2-propanol, acetonitrile, 2-methoxyethanol, 2-ethoxyethanol, 2-buthoxyethanol, and ethylene glycol) and two binary mixed solvents (methanol + ethanol and 2-methoxyethanol + ethanol) were been measured by high performance liquid chromatography at temperatures ranging from (288.15 to 328.15) K and atmospheric pressure. From the experimental data, it was found that the solubility increased with the rising temperature in all studied solvents. The obtained values for the solubility of rebaudioside A in pure and mixed binary solvents were correlated with modified Apelblat and nonrandom two-liquid models. The results showed that these models can accurately correlate the solubility data obtained in this work. Furthermore, the crystal habits of rebaudioside A in selected solvents were studied.
研究领域:
食品科学
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物理化学
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产品关联
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2019
·Vol. 13
·期 2
·1159-1165
·
IF 1.415
Musa Abubakr,Jiang Bo,Ma Haile,Gasmalla Mohammed A. A.,Abdalhai Mandour H.,Al-alfarga Ammar
In this work, alternansucrase enzyme from Leuconostoc citreum SK24.002 was used to produce di-glycosyl-stevioside through acceptor reaction. This was detected by high-performance liquid chromatography (HPLC) and was isolated using AB-8 macroporous resin and semi-preparative HPLC. With the help of mass spectroscopy, 1D NMR (H 1 and C 13 ) and 2D NMR (COSY, HMQC, HMBC) the di-glycosyl-stevioside structure was identified to be 13-{[α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-β-D glucopyranosyl-(1→2)-β-D-glucopyranosyl]oxy} kaur-16-en-19-oic acid β-D glucopyranosyl eater. In order to increase the modified stevioside (di-glycosyl-stevioside) product and reduce production time, the impact of velocity of shaking on the di-glycosyl-stevioside production was also evaluated, using 75–150 rpm for 24 h at 25 °C. The results showed that the di-glycosyl-stevioside production increased significantly (p < 0.05) from 0.35 ± 0.0.01 mg/mL for 75 rpm to 1.0667 ± 0.0152 mg/mL for 150 rpm at 6 h during the glycosylation reaction.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
Oncology Letters
·
2018
·Vol. 15
·期 4
·5250-5256
·
IF 1.664
Jun‑Ming Chen,Jue Zhang,Yong‑Mei Xia,Xiao‑Xia Wang,Jian Li
Steviol is the colonic metabolite of the natural sweetener steviol glycosides. It does not diffuse to the blood and the half maximal inhibitory concentration of steviol is longer compared with that of current chemotherapy agents, including 5‑fluorouracil and doxorubicin. The present study demonstrated that steviol inhibits the proliferation of the human osteosarcoma U2OS cell line in a dose‑ and time‑dependent manner, and that the inhibition rate is comparative with that of doxorubicin and 5‑fluorouracil. The mechanism of this anticancer activity is also investigated. The results indicated that steviol inhibits U2OS cells through inducing G1 phase cell cycle arrest, downregulating the ability of colony formation via a mitochondrial apoptotic pathway, which was indicated by an increase of the Bax/Bcl‑2 ratio and activation of cyclin‑dependent kinase inhibitor 1, tumor protein 53 and cyclin‑dependent kinase; whereas a Survivin and Caspase 3‑independent mechanism was involved. Considering that steviol appears minimally in the plasma during metabolism, and possesses a median lethal dose of 100-fold greater compared with that of 5‑fluorouracil, it may become a potential chemotherapy agent.
研究领域:
肿瘤学
·
药理学
·
代谢
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR MODELING
·
2021
·Vol. 27
·期 9
·1-12
·
IF 1.81
Zhu Yajie,Ding Yan,Tian Dongxu,Li Yan,Zhuang Linwu,Wang Yinpeng,Xiao Wei,Zhu Jingbo
In this paper, a novel molecularly imprinted polymer (MIP) for specific adsorption of steviol glycosides was designed, and the imprinting mechanism of self-assembly system between template and monomers was clearly explored. Firstly, steviol (STE) was chosen as dummy template, and the density functional theory (DFT) at B3LYP/6–31 + G (d, p) level was used to select monomers, imprinting molar ratios, solvents, and cross-linking agents. The selectivity to five steviol glycosides was also calculated. Importantly, reduced density gradient (RDG) theory combined with atom in molecules (AIM) and infrared spectrum (IR) was applied to investigate the bonding situation and the nature of noncovalent interaction in self-assembly system. The theoretical designed results showed that the template which interacts with acrylic acid (AA) has the minimum binding energy, and the complex with the molar ratio of 1 : 4 has the most stable structure. Toluene (TL) and ethylene glycol dimethacrylate (EGDMA) were chosen as the optimal solvent and cross-linking agent, respectively. Five hydrogen bonds formed in the self-assembly system are the key forces at the adsorption sites of MIPs through the RDG and AIM analyses. The MIPs were synthesized by theoretical predictions, and the results showed that the maximum adsorption capacity towards dulcoside A is 26.17 mg/g. This work provided a theoretical direction and experimental validation for deeper researches of the MIPs for steviol glycosides. In addition, the method of RDG theory coupled with AIM and IR also could be used to analyze other imprinting formation mechanisms systematically.
研究领域:
食品科学
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材料科学
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理论化学
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DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 16
·3416
·
IF 4.6
Zidan Liu,Linyu Luo,Zhiqiang Ding,Weihao Long,Tolbert Osire,Qiong Li,Qianfeng Chen,Mengfei Long
Stevioside, a natural high-intensity sweetener, is widely employed across the food, pharmaceutical, and daily chemical industries due to its intense sweetness and health benefits. However, traditional extraction and purification processes for steviol glycosides fromStevia rebaudianaare plagued by low efficiency, high energy consumption, substantial environmental impact, and inconsistent product quality. This study systematically optimized the extraction, decolorization, decontamination, and desalting processes to overcome these challenges. The extraction method was refined using 20% ethanol as the solvent, an optimal temperature of 50 °C, and a 1:10 material-to-liquid ratio, increasing the steviol glycoside yield from 32.0% to 49.1%. Decolorization employing a combination of resins D940 and T5 achieved decolorization rates of 89–92% with minimized steviol glycoside loss, surpassing the non-selective adsorption limitations of activated carbon. For decontamination, calcium hydroxide (Ca(OH)2) outperformed diatomaceous earth, attaining a 98% protein removal rate while maintaining steviol glycoside loss below 20%. The desalting resin LXP-016 demonstrated superior performance at 40 °C, enhancing the ability of ionic impurity removal. These optimizations collectively improve the efficiency, sustainability, and quality of steviol glycoside production, offering a promising framework for industrial-scale applications.
研究领域:
植物生物技术
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食品科学
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药学
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化学工程
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Zhuqing Dai,Jiangfeng Song,Ye Chen,Lei Feng,Yayuan Xu,Dajing Li,Caie Wu,Zhongyuan Zhang,Jun Liu
This study aims to develop novel lutein nanoparticles encapsulized by stevioside (LUT-STE, 165 ± 2 nm average particles size) and systematically evaluate its bioavailability. Multiple spectroscopy and NMR analyses showed lutein and stevioside could interact through hydrogen bonds, C H π interaction and van der Waals forces. Molecular docking simulation showed lutein was well distributed in the hydrophobic cavity of stevioside. Analyzed by Caco-2 cellular models, the transported amount of LUT-STE was 2.39 times that of lutein in 120 min with a P app (B → A)/ P app (A → B) value of 0.63 ± 0.04. Nystatin and dynasore significantly reduced the cellular uptake of LUT-STE by 41.3% and 57.7%, respectively. Compared with free lutein, LUT-STE increased the C max in mice plasma by 5.01-fold and promoted the accumulation in multiple organs. LUT-STE promoted the protein expressions of CD36, NPC1L1 and PPARγ in both cell and animal models. In conclusion, stevioside entrapment significantly promote the bioavailability of lutein through multiple transmembrane pathways.
研究领域:
药学
·
营养学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR CHEMISTRY AND PHYSICS
·
2021
·Vol. 222
·期 17
·2100145
·
IF 2.527
Qiaoge Fu,Nana Chen,Guanyu Wang,Ruili Guo
The poly (ethylene glycol dimethacrylate- co -vinylphenylboronic acid) P(EGDMA- co -VPBA) adsorbents are prepared via reversible addition-fragmentation chain transfer (RAFT) precipitation polymerization with 4-vinylphenylboronic acid (VPBA) as the functional monomer, and used for selective adsorption of steviol glycosides (SGs). The boron content and pore structure of the P(EGDMA- co -VPBA) adsorbent are adjusted by changing the polymerization conditions. The adsorption results show that the pore structure of the P(EGDMA- co -VPBA) affects the adsorption capacity to SGs, the presence of boric acid functional groups shows favorable adsorption selectivity for stevioside (STV), and the adsorption selectivity is proportional to the content of boric acid in P(EGDMA- co -VPBA) adsorbents. The adsorption performance of P(EGDMA- co -VPBA) toward SGs is the best when the ratio of monomer to cross-linker is 1:50 and the solvent is acetonitrile. In the SG solution (6.0 mg mL −1 , C STV / C RA = 1:1), the adsorption capacities and adsorption selectivity ( α STV/RA ) of P(EGDMA- co -VPBA) to SGs are 113.20 mg g –1 and 4.99, respectively. The adsorption kinetics and isotherm data obey pseudo-second-order kinetic model and Langmuir isotherm model, respectively. The boric acid copolymers with VPBA as the monomer prepared by the one-step method can become a promising material for efficient separation and purification of SGs.
研究领域:
化学工程
·
食品科学
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Fan Wang,Jinling Zhan,Rongrong Ma,Yaoqi Tian
Bioactive compounds are frequently incorporated into polysaccharides (e.g., starch) to form active biodegradable films for food packaging, but some of them are water insoluble (e.g., curcumin , CUR) that will make the films have undesirable performance. Herein, CUR was successfully solubilized into the aqueous starch film solution by steviol glycoside (STE, a natural sweetener)-based solid dispersion. The mechanisms of solubilization and film formation were explored through molecular dynamic simulation and various characterization methods. The results showed that the amorphous state of CUR combined with micellar encapsulation of STE achieved the solubilization of CUR. STE and starch chains cooperated to form the film via hydrogen bonding , while CUR was uniformly and densely distributed within the film in the form of needle-like microcrystals. The as-prepared film exhibited high flexibility, great moisture barrier, and excellent UV barrier (UV transmittance : ∼0 %). Compared with the film containing CUR alone, the as-prepared film possessed higher release efficiency, antibacterial activity , and pH response sensitivity due to the assistance of STE. Hence, the introduction of STE-based solid dispersion can simultaneously improve the biological and physical properties of starch films, which provides a green, nontoxic, and facile strategy for the perfect integration of hydrophobic bioactive compounds and polysaccharide-based films.
研究领域:
食品科学
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）