INDUSTRIAL CROPS AND PRODUCTS
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2021
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IF 5.645
Yijuan Chen,Jiaxuan Luo,Nan Zhang,Wenjuan Yu,Jiexian Jiang,Guanghui Dai
Plant essential oils are the powerful candidates to develop natural insect management agents. Their chemical compounds along with their biological activity are often affected by many factors. However, the relationships between the composition and bioactivity of the compounds are not well understood. In this study, eight plant essential oils were screened, and the main compounds from the most effective oil showing insecticidal properties against third-instar larvae of the beet armyworm ( Spodoptera exigua Hübner) were evaluated for comparative and synergistic interactions by topical application. The results indicated that Salvia hispanica L. essential oil showed significant insecticidal activity against S. exigua compared with the other seven essential oils. Fifteen major compounds were identified from S. hispanica via gas chromatography-mass spectrometry, with the most abundant compound (α-thujone) possessing the highest insecticidal activity. Several combinations of the four major compounds (α-thujone, (+)-camphor, 1,8-cineole, and α-caryophyllene) from S. hispanica exhibited synergistic insecticidal activities. This study demonstrates the potential of S. hispanica essential oil and α-thujone as effective botanical insecticides in the control of beet armyworm and provides a better understanding of essential oil compound-pest interactions.
研究领域:
植物学
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昆虫学
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农业科学
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产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2022
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IF 4.966
Fen Li,Xueyan Gong,Linlin Yuan,Xuelian Pan,Haifeng Jin,Rongcai Lu,Shaoying Wu
Liriomyza trifolii , which has been recently prevalent in China, harms more than 300 plant species, especially cowpea in Hainan. This pest also affects the quality and production of vegetables in winter. Indoxacarb is the first commercial oxadiazine pesticide , which is a new efficient insecticide used to control pests of Diptera, including L. trifolii . The unique mechanism of indoxacarb is that indenyl is transformed into N-demethoxycarbonyl metabolite (DCJW) in insects and acts on inactivated sodium channel; DCJW could then destroy the conduction of nerve impulses , which leads to movement disorders, feeding stoppage, paralysis, and eventually the death of pests. The field population of L. trifolii developed resistance by 769 times higher than the sensitive population in Sanya, Hainan. Results revealed the existence of a mutation (i.e., V1848I) in the sixth transmembrane segment of Domain IV of the sodium channel in the field population. The homozygous resistant genotype frequency for the V1848I mutation was 10–15% among the three field-collected populations. This paper reports for the first time the presence of the kdr mutation V1848I in resistant populations of L. trifolii to indoxacarb. The present study will contribute to the understanding of the evolution of indoxacarb resistance and contribute to the development of resistance management practices for winter vegetables in Hainan.
研究领域:
昆虫学
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农药科学
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农业科学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Huanhuan Dong,Yafei Qu,Ertao Li,Jing Luo,Ibrahim Osman,Zhimin Wang,Hongnian Li,Kebin Li,Jiao Yin
The beet webworm, Loxostege sticticalis, whose oviposition behavior significantly affects population quantity and causes crop losses, has poorly understood olfactory mechanisms governing this behavior. Odorant receptors (ORs) play a critical role in the insect olfactory system. Here, three ORs (LstiOR1, LstiOR2, and LstiOR3) were cloned, with all highly expressed in female antennae. The two-electrode voltage clamp recording revealed that only LstiOR3 displayed broad tuning to nine host plant volatiles, especially linalool. Electroantennogram recordings confirmed that the nine ligands induced electrophysiological responses, with particularly strong responses observed for linalool in female antennae. Subsequently, dual-fragment RNA interference (RNAi) effectively silenced LstiOR3, significantly reducing the electrophysiological and behavioral responses of L. sticticalis to linalool. Additionally, molecular docking showed that LstiOR3 binds strongly to linalool through hydrogen bonds at VAL-199 and GLY-203. In summary, our studies elucidate the specific biological function of LstiOR3 via linalool detection in oviposition site selection.
研究领域:
昆虫学
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分子生物学
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神经生物学
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农业科学
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化学生态学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2018
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IF 3.44
Xuegui Wang,Xing Xiang,Huiling Yu,Shuhua Liu,Yong Yin,Peng Cui,Yaqiong Wu,Jing Yang,Chunxian Jiang,Qunfang Yang
The beet armyworm Spodoptera exigua , a major pest affecting numerous cultivated crops in China, has developed a serious resistance to many traditional chemical insecticides . The resistance levels of the field-collected populations from different districts in Sichuan Province, China, to nine insecticides were detected with a diet-incorporation bioassay. Compared to the Lab-ZN strain, five (in 2014) and three (in 2016) field populations displayed either high or extremely high levels of resistance to beta-cypermethrin. All the field populations collected in 2014 were susceptible to emamectin benzoate, hexaflumuron, methoxyfenozide, chlorantraniliprole, cyantraniliprole and indoxacarb but exhibited low or moderate levels of resistance to abamectin . The resistances of field populations collected in 2016 were significantly higher than two years earlier, especial for chlorantraniliprole and cyantraniliprole with RRs rising from 173.4- to 582.6-fold and 175.3- to 287.6-fold, respectively, even though the field populations had retained moderate or low levels of resistance to chlorpyrifos and hexaflumuron. The synergism experiment revealed that the resistance of the LS16 population to beta-cypermethrin may be mainly related to cytochrome P450 monooxygenases (P450s), which was responsible for the highest increase ratio of 37.97-fold, for piperonyl butoxide , rather than either carboxylesterase (CarE) or glutathione S-transferase (GST). The cytochrome P450 ethoxycoumarin O -deethylase activity of the LS16 population was also the strongest among the treatments ( P < 0.05). Non-denaturing polyacrylamide gel electrophoresis (native PAGE) indicated that enhanced E11, E13 and E15-E16 bands in the LS16 population likely contribute to the development of resistance to beta-cypermethrin.
研究领域:
昆虫学
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害虫管理
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生物化学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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